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The map label for this gene is panB

Identifier: 114797775

GI number: 114797775

Start: 780833

End: 781660

Strand: Direct

Name: panB

Synonym: HNE_0771

Alternate gene names: 114797775

Gene position: 780833-781660 (Clockwise)

Preceding gene: 114797253

Following gene: 114799082

Centisome position: 21.07

GC content: 64.61

Gene sequence:

>828_bases
ATGTCAAAACAGACCCAGACGACCCGCAAGACCGTCAAGGACATCGCCGCTGCCAAAGGGGCAACGCCACTTGTCATGTT
GACGGCCTATGACGCCCCGACCGCCGCGATCCTTGATCCCCATTGTGACATCCTGCTGGTGGGCGACAGCCTCGGCATGG
TGGTCCATGGCCTGCCGTCCACGGTTGGCGTCACGATGGAAATGATGATCCTCCACGGCCAGGCCGTCATGCGCGGCGCG
TCCCAGGCGATGGTCGTGGTCGATATGCCCTTCGGCTCGTATGAAACCAACGCTGATCAGGCCTTCCTGAACGCGGTGCG
GATCATGAAGGAAACCGGCTGCCAGGCCATCAAGATCGAAAGCGGCGCCTATGCTGCCGGCCAGATCGCCCATCTCGTCG
AGCGCGGCATTCCGGTCATGGGGCATATCGGCCTGCGCCCGCAGGCAATCAACGTCGATGGCGGCTTCCGCGCCAAGGGC
CGCACCGAAGATGAACGCGACCGCGTCATCGCCGAGGCCCGCGCCGCGGCCGATGCCGGCGCCTTCTGCATCGTCATCGA
AGGCGTCGCCGAGGATCTTGCCGCCGCCATCACCGCCGAAGTCAGCTGCCCGACCATCGGGATCGGCGCCTCGGCGGCCT
GTGACGGCCAGGTTCTCGTCACCCAGGACATGCTCGGCCTGTTCGACTGGACGCCCAAATTCGTCCGCCGCTACGCCGAT
CTGCGCGAAGTGGTCGATAAAGCCGCCGCTGAATACGCTGCAGATGTGCGCGCCCGGCGCTTCCCCGGCACGGCTGAAAC
CTACAGCCTGCGAAAACAAGGGTCTTAA

Upstream 100 bases:

>100_bases
GGTTCCACCCCATCCTGACGGGCATGCCCGCGCTGCCCGCCTGAGCCCGGCCGCAAAGGCATTGCTTGCCCGCCCGGTCC
TGACCGGGTAATCGCAGGCC

Downstream 100 bases:

>100_bases
CAGCGCTGATGCGTTGCAGCCTCCGCATGGGCCGTCTAGTTTGCGCCCGAGTCCCCAACAGTCCCGCCGGAGCCCCAGCG
TGAGCGATATTTTCACTGAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSKQTQTTRKTVKDIAAAKGATPLVMLTAYDAPTAAILDPHCDILLVGDSLGMVVHGLPSTVGVTMEMMILHGQAVMRGA
SQAMVVVDMPFGSYETNADQAFLNAVRIMKETGCQAIKIESGAYAAGQIAHLVERGIPVMGHIGLRPQAINVDGGFRAKG
RTEDERDRVIAEARAAADAGAFCIVIEGVAEDLAAAITAEVSCPTIGIGASAACDGQVLVTQDMLGLFDWTPKFVRRYAD
LREVVDKAAAEYAADVRARRFPGTAETYSLRKQGS

Sequences:

>Translated_275_residues
MSKQTQTTRKTVKDIAAAKGATPLVMLTAYDAPTAAILDPHCDILLVGDSLGMVVHGLPSTVGVTMEMMILHGQAVMRGA
SQAMVVVDMPFGSYETNADQAFLNAVRIMKETGCQAIKIESGAYAAGQIAHLVERGIPVMGHIGLRPQAINVDGGFRAKG
RTEDERDRVIAEARAAADAGAFCIVIEGVAEDLAAAITAEVSCPTIGIGASAACDGQVLVTQDMLGLFDWTPKFVRRYAD
LREVVDKAAAEYAADVRARRFPGTAETYSLRKQGS
>Mature_274_residues
SKQTQTTRKTVKDIAAAKGATPLVMLTAYDAPTAAILDPHCDILLVGDSLGMVVHGLPSTVGVTMEMMILHGQAVMRGAS
QAMVVVDMPFGSYETNADQAFLNAVRIMKETGCQAIKIESGAYAAGQIAHLVERGIPVMGHIGLRPQAINVDGGFRAKGR
TEDERDRVIAEARAAADAGAFCIVIEGVAEDLAAAITAEVSCPTIGIGASAACDGQVLVTQDMLGLFDWTPKFVRRYADL
REVVDKAAAEYAADVRARRFPGTAETYSLRKQGS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=246, Percent_Identity=39.4308943089431, Blast_Score=165, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319653, Length=263, Percent_Identity=38.7832699619772, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_HYPNA (Q0C444)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759499.1
- ProteinModelPortal:   Q0C444
- SMR:   Q0C444
- STRING:   Q0C444
- GeneID:   4289619
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0771
- NMPDR:   fig|228405.5.peg.754
- TIGR:   HNE_0771
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- PhylomeDB:   Q0C444
- ProtClustDB:   PRK00311
- BioCyc:   HNEP81032:HNE_0771-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29099; Mature: 28968

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 88-88 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQTQTTRKTVKDIAAAKGATPLVMLTAYDAPTAAILDPHCDILLVGDSLGMVVHGLPS
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEECCCCCCEEECCCC
TVGVTMEMMILHGQAVMRGASQAMVVVDMPFGSYETNADQAFLNAVRIMKETGCQAIKIE
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEC
SGAYAAGQIAHLVERGIPVMGHIGLRPQAINVDGGFRAKGRTEDERDRVIAEARAAADAG
CCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCC
AFCIVIEGVAEDLAAAITAEVSCPTIGIGASAACDGQVLVTQDMLGLFDWTPKFVRRYAD
EEEEEEECHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHH
LREVVDKAAAEYAADVRARRFPGTAETYSLRKQGS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SKQTQTTRKTVKDIAAAKGATPLVMLTAYDAPTAAILDPHCDILLVGDSLGMVVHGLPS
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEECCCCCCEEECCCC
TVGVTMEMMILHGQAVMRGASQAMVVVDMPFGSYETNADQAFLNAVRIMKETGCQAIKIE
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEC
SGAYAAGQIAHLVERGIPVMGHIGLRPQAINVDGGFRAKGRTEDERDRVIAEARAAADAG
CCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCC
AFCIVIEGVAEDLAAAITAEVSCPTIGIGASAACDGQVLVTQDMLGLFDWTPKFVRRYAD
EEEEEEECHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHH
LREVVDKAAAEYAADVRARRFPGTAETYSLRKQGS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA