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The map label for this gene is gyrB [H]

Identifier: 114797774

GI number: 114797774

Start: 547859

End: 550303

Strand: Reverse

Name: gyrB [H]

Synonym: HNE_0552

Alternate gene names: 114797774

Gene position: 550303-547859 (Counterclockwise)

Preceding gene: 114799571

Following gene: 114800397

Centisome position: 14.85

GC content: 63.35

Gene sequence:

>2445_bases
ATGGCCGAAGACATCAATCCCGAAGCCCCCGAAGAGGGCGGCGAAGGCGAGTATGGCGCGGGCTCCATCAAGGTCCTCAA
GGGCCTTGATGCCGTGCGCAAGCGTCCCGGCATGTACATCGGCGACACCGATGACGGCTCCGGCCTCCACCACATGATCT
ACGAGGTCGTCGACAACTCCATCGACGAAGCCCTCGCCGGCCATGCCGACCATGTGACGGTCACCCTCTTCCCCGATGGC
TCGGCCGAAGTCACCGACAATGGCCGCGGCATCCCTGTCGGCCTCCACCCCTCCGAAGGCGTCTCGGCGGCCGAGGTCAT
CATGACCCAGCTGCACGCCGGCGGTAAGTTCGACCAGAACTCCTACAAGGTCTCCGGCGGCCTTCACGGCGTCGGCGTCT
CGGTCGTCAACGCCCTGTCTGACTGGCTGGAGCTGACCATCCACCGCGAAGGCAAGGAACATTTCGTCCGCTTCGTCGAG
GGTGGCCGCGTCGAGGCTCCGCTCGTCACGCGCGGACCTTCCCCGAAGCGCGAAAACGGCAAGGCCTATACCGGCACCGC
CCTGCGCTTCCTCGCCTCCACGTCAACGTTCACGATGACCGACTATGACCGCAAGACGCTCGAGCATCGCCTGCGCGAGC
TCGCCTTCCTCAACAGCGGTGTAAAGATCATCTTCCGGGATGAGCGCGAGGCTGAGCCCTACGAAATCATCCTGGAATAT
GAAGGCGGCGTGAAAGCCTTCGTCCAGCATATCGACCGGCAGAAATCCTCCCTCATCGGAGAGCCCATCTATGTCATCGG
CGAGAAAGACGGCATCACCGTCGAAGCCGCGCTGGAATGGACCGACACCTATCACGAATCCGTCCTCTGCTTCACCAACA
ACATCCCCCAGCGCGATGGCGGCACCCACCTTGCCGGCTTCCGCGGCGCGCTCACGCGCATCATCAACAAATACGCCAAC
GAAACCGGCGCCGCGAAAAAATCGAAAGTCGAGATTTCCGGCGATGACGCCCGCGAAGGACTGACCTGCGTGCTCTCGGT
CAAGGTGCCCGACCCGAAGTTCAGCTCCCAGACGAAAGACAAGCTCGTCTCCTCCGAGGTGCGCCCGGTTGTCGAAAGCC
TGATCAACGAGAAGCTGGGCGAGTGGTTCGAAGAGAACCCCAAGAAAGCCCAGATCATCATGGAGAAGGTCGTCGAGGCT
GCTGCCGCCCGCGAAGCCGCCCGCAAGGCGCGCGAGCTGACACGCCGCAAATCGGCGCTCGACATCACCTCCCTTCCGGG
GAAGCTCGCGGACTGCCAGGAAAAAGACCCGGCGAAATCCGAAATCTTCATCGTCGAGGGTGACTCGGCCGGTGGCTCTG
CCAAACAGGGCCGCAGCCGGGACAACCAGGCCATCCTCCCCCTGCGCGGCAAGATCCTCAACGTTGAGCGCGCGCGCTTT
GACAAGATGCTCGGCTCCGATCAGGTCGGTACACTGATCATGGCGCTCGGCGCCGGCATTGGCCGGGATGAGTTCAACAT
TGCCAAGCTGCGCTATCACAAGATCATCATCATGACAGATGCTGACGTTGACGGCGCCCACATCCGCACCCTGCTGCTCA
CCTTCTTCTATCGTCAGATGCCGGAGATTATCGAAAACGGTTATCTCTACATCGCCCAGCCCCCGCTCTACAAAGTCACG
CGCGGCCGGTCTGAGCGCTATGTGAAGGACGACGCAGAGCTGGAATCCTACCTCATCGAGGAAGGCACCACCGGGGAAAC
CCTGATCCTCGCCGATGGCACCCAGATTGCCGGGGCGGACCTGCGCGAGCAGGTCCATCAGGCGGCGCAATTCCGCGCCA
GCCTGCGCCGCCTCGCCCTGCGCGCGCCGGCTGACCTGATCGAGCACGCCGCCCTCACCGGCGCGATGAAGCCCGGCGCC
GGACAGGACGAGGCCGACGCGACCGCCGCCCGCCTCAACCGCCTCGCCGAAGAAGGCGAAGACACCTGGGTCGGCCGCTT
CGTGGAAGGCGCGCTTGTGCTTCAGCGCACCGTGCGCGGCGTGGATGAAAATGCCGTCCTGCCCCCGGCCCTGATGGCCA
GTCAGGACGCCATCCGCCTCTCCGATCGGGCCGTCGGCCTGCAAGAGCTTTATGCGGAGCAATCGATCCTGCGCCATGAA
AAAGGCGGCGAAATTTCCGTCAACGGACCGTCCACACTGCTCGGCGCGGTACTGGAATCAGGCCGCAAAGGCCTGAAAAT
CCAGCGCTACAAGGGTCTGGGCGAAATGAACGCCAGCCAGCTCTGGGAAACCACCCTCGACGCCAACGCCCGCACGCTCC
TGCAGGTCAAGGTCGAGCACGCCGACGAAGCCGACGAACTCTTCACCAAACTGATGGGCGACGTGGTCGAACCCCGCCGG
GAGTTCATCGAAGAGTTTGCGCTGGAAGCCGAAGTCGACGTCTAG

Upstream 100 bases:

>100_bases
TTGCGGCACTGTAAAATGATTGCCGTTCCGGTTCCCAGCGGAGCGTAAATCCCCTATATTTAAGGGGAATTTCGAAGAAT
CATCGAGAAAGCAGTTCCCC

Downstream 100 bases:

>100_bases
CCCTTCCCCGTGTCATCCCGGACGGCCAAAGGCCGATCCGGGATCTTCTCGCAATCTTGCAAACGCAACCATCCCCGCCC
TTGCGCGTTAAATCCTCCGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MAEDINPEAPEEGGEGEYGAGSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMIYEVVDNSIDEALAGHADHVTVTLFPDG
SAEVTDNGRGIPVGLHPSEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELTIHREGKEHFVRFVE
GGRVEAPLVTRGPSPKRENGKAYTGTALRFLASTSTFTMTDYDRKTLEHRLRELAFLNSGVKIIFRDEREAEPYEIILEY
EGGVKAFVQHIDRQKSSLIGEPIYVIGEKDGITVEAALEWTDTYHESVLCFTNNIPQRDGGTHLAGFRGALTRIINKYAN
ETGAAKKSKVEISGDDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLINEKLGEWFEENPKKAQIIMEKVVEA
AAAREAARKARELTRRKSALDITSLPGKLADCQEKDPAKSEIFIVEGDSAGGSAKQGRSRDNQAILPLRGKILNVERARF
DKMLGSDQVGTLIMALGAGIGRDEFNIAKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPEIIENGYLYIAQPPLYKVT
RGRSERYVKDDAELESYLIEEGTTGETLILADGTQIAGADLREQVHQAAQFRASLRRLALRAPADLIEHAALTGAMKPGA
GQDEADATAARLNRLAEEGEDTWVGRFVEGALVLQRTVRGVDENAVLPPALMASQDAIRLSDRAVGLQELYAEQSILRHE
KGGEISVNGPSTLLGAVLESGRKGLKIQRYKGLGEMNASQLWETTLDANARTLLQVKVEHADEADELFTKLMGDVVEPRR
EFIEEFALEAEVDV

Sequences:

>Translated_814_residues
MAEDINPEAPEEGGEGEYGAGSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMIYEVVDNSIDEALAGHADHVTVTLFPDG
SAEVTDNGRGIPVGLHPSEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELTIHREGKEHFVRFVE
GGRVEAPLVTRGPSPKRENGKAYTGTALRFLASTSTFTMTDYDRKTLEHRLRELAFLNSGVKIIFRDEREAEPYEIILEY
EGGVKAFVQHIDRQKSSLIGEPIYVIGEKDGITVEAALEWTDTYHESVLCFTNNIPQRDGGTHLAGFRGALTRIINKYAN
ETGAAKKSKVEISGDDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLINEKLGEWFEENPKKAQIIMEKVVEA
AAAREAARKARELTRRKSALDITSLPGKLADCQEKDPAKSEIFIVEGDSAGGSAKQGRSRDNQAILPLRGKILNVERARF
DKMLGSDQVGTLIMALGAGIGRDEFNIAKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPEIIENGYLYIAQPPLYKVT
RGRSERYVKDDAELESYLIEEGTTGETLILADGTQIAGADLREQVHQAAQFRASLRRLALRAPADLIEHAALTGAMKPGA
GQDEADATAARLNRLAEEGEDTWVGRFVEGALVLQRTVRGVDENAVLPPALMASQDAIRLSDRAVGLQELYAEQSILRHE
KGGEISVNGPSTLLGAVLESGRKGLKIQRYKGLGEMNASQLWETTLDANARTLLQVKVEHADEADELFTKLMGDVVEPRR
EFIEEFALEAEVDV
>Mature_813_residues
AEDINPEAPEEGGEGEYGAGSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMIYEVVDNSIDEALAGHADHVTVTLFPDGS
AEVTDNGRGIPVGLHPSEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELTIHREGKEHFVRFVEG
GRVEAPLVTRGPSPKRENGKAYTGTALRFLASTSTFTMTDYDRKTLEHRLRELAFLNSGVKIIFRDEREAEPYEIILEYE
GGVKAFVQHIDRQKSSLIGEPIYVIGEKDGITVEAALEWTDTYHESVLCFTNNIPQRDGGTHLAGFRGALTRIINKYANE
TGAAKKSKVEISGDDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLINEKLGEWFEENPKKAQIIMEKVVEAA
AAREAARKARELTRRKSALDITSLPGKLADCQEKDPAKSEIFIVEGDSAGGSAKQGRSRDNQAILPLRGKILNVERARFD
KMLGSDQVGTLIMALGAGIGRDEFNIAKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPEIIENGYLYIAQPPLYKVTR
GRSERYVKDDAELESYLIEEGTTGETLILADGTQIAGADLREQVHQAAQFRASLRRLALRAPADLIEHAALTGAMKPGAG
QDEADATAARLNRLAEEGEDTWVGRFVEGALVLQRTVRGVDENAVLPPALMASQDAIRLSDRAVGLQELYAEQSILRHEK
GGEISVNGPSTLLGAVLESGRKGLKIQRYKGLGEMNASQLWETTLDANARTLLQVKVEHADEADELFTKLMGDVVEPRRE
FIEEFALEAEVDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=554, Percent_Identity=24.5487364620939, Blast_Score=139, Evalue=2e-32,
Organism=Homo sapiens, GI19913406, Length=572, Percent_Identity=24.6503496503496, Blast_Score=137, Evalue=6e-32,
Organism=Escherichia coli, GI48994957, Length=807, Percent_Identity=56.0099132589839, Blast_Score=827, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=567, Percent_Identity=39.5061728395062, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535065, Length=585, Percent_Identity=23.4188034188034, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=291, Percent_Identity=24.3986254295533, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI212645657, Length=141, Percent_Identity=31.9148936170213, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=585, Percent_Identity=24.1025641025641, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136538, Length=548, Percent_Identity=25.7299270072993, Blast_Score=137, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89119; Mature: 88988

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDINPEAPEEGGEGEYGAGSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMIYEVVDNS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHH
IDEALAGHADHVTVTLFPDGSAEVTDNGRGIPVGLHPSEGVSAAEVIMTQLHAGGKFDQN
HHHHHCCCCCEEEEEEECCCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
SYKVSGGLHGVGVSVVNALSDWLELTIHREGKEHFVRFVEGGRVEAPLVTRGPSPKRENG
CEEECCCCCHHHHHHHHHHHHHHHEEHCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCCC
KAYTGTALRFLASTSTFTMTDYDRKTLEHRLRELAFLNSGVKIIFRDEREAEPYEIILEY
CEEEHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEE
EGGVKAFVQHIDRQKSSLIGEPIYVIGEKDGITVEAALEWTDTYHESVLCFTNNIPQRDG
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECHHHHHHCEEEEEECCCCCCCC
GTHLAGFRGALTRIINKYANETGAAKKSKVEISGDDAREGLTCVLSVKVPDPKFSSQTKD
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCHHHH
KLVSSEVRPVVESLINEKLGEWFEENPKKAQIIMEKVVEAAAAREAARKARELTRRKSAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DITSLPGKLADCQEKDPAKSEIFIVEGDSAGGSAKQGRSRDNQAILPLRGKILNVERARF
CHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEECHHHHHH
DKMLGSDQVGTLIMALGAGIGRDEFNIAKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQM
HHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHH
PEIIENGYLYIAQPPLYKVTRGRSERYVKDDAELESYLIEEGTTGETLILADGTQIAGAD
HHHHHCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHH
LREQVHQAAQFRASLRRLALRAPADLIEHAALTGAMKPGAGQDEADATAARLNRLAEEGE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
DTWVGRFVEGALVLQRTVRGVDENAVLPPALMASQDAIRLSDRAVGLQELYAEQSILRHE
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHC
KGGEISVNGPSTLLGAVLESGRKGLKIQRYKGLGEMNASQLWETTLDANARTLLQVKVEH
CCCEEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEECC
ADEADELFTKLMGDVVEPRREFIEEFALEAEVDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEDINPEAPEEGGEGEYGAGSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMIYEVVDNS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHH
IDEALAGHADHVTVTLFPDGSAEVTDNGRGIPVGLHPSEGVSAAEVIMTQLHAGGKFDQN
HHHHHCCCCCEEEEEEECCCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
SYKVSGGLHGVGVSVVNALSDWLELTIHREGKEHFVRFVEGGRVEAPLVTRGPSPKRENG
CEEECCCCCHHHHHHHHHHHHHHHEEHCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCCC
KAYTGTALRFLASTSTFTMTDYDRKTLEHRLRELAFLNSGVKIIFRDEREAEPYEIILEY
CEEEHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEE
EGGVKAFVQHIDRQKSSLIGEPIYVIGEKDGITVEAALEWTDTYHESVLCFTNNIPQRDG
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECHHHHHHCEEEEEECCCCCCCC
GTHLAGFRGALTRIINKYANETGAAKKSKVEISGDDAREGLTCVLSVKVPDPKFSSQTKD
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCHHHH
KLVSSEVRPVVESLINEKLGEWFEENPKKAQIIMEKVVEAAAAREAARKARELTRRKSAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DITSLPGKLADCQEKDPAKSEIFIVEGDSAGGSAKQGRSRDNQAILPLRGKILNVERARF
CHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEECHHHHHH
DKMLGSDQVGTLIMALGAGIGRDEFNIAKLRYHKIIIMTDADVDGAHIRTLLLTFFYRQM
HHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHH
PEIIENGYLYIAQPPLYKVTRGRSERYVKDDAELESYLIEEGTTGETLILADGTQIAGAD
HHHHHCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHH
LREQVHQAAQFRASLRRLALRAPADLIEHAALTGAMKPGAGQDEADATAARLNRLAEEGE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
DTWVGRFVEGALVLQRTVRGVDENAVLPPALMASQDAIRLSDRAVGLQELYAEQSILRHE
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHC
KGGEISVNGPSTLLGAVLESGRKGLKIQRYKGLGEMNASQLWETTLDANARTLLQVKVEH
CCCEEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEECC
ADEADELFTKLMGDVVEPRREFIEEFALEAEVDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]