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The map label for this gene is fadA [H]

Identifier: 114797516

GI number: 114797516

Start: 675735

End: 676940

Strand: Direct

Name: fadA [H]

Synonym: HNE_0671

Alternate gene names: 114797516

Gene position: 675735-676940 (Clockwise)

Preceding gene: 114798681

Following gene: 114797137

Centisome position: 18.24

GC content: 63.52

Gene sequence:

>1206_bases
ATGCCCGAAGCCTATATCTACGACGCCGTGCGCACGCCCCGCGGCAAAGGCAAATCCTCCGGCGCGCTGCATGAAATCAC
CGCGCTCAGCCTCGGCACGCAGGTGCTGCAGGCCATCCGTGACCGGAACAATCTCGACACCTCCAAGGTCGATGACGTGG
TCTTCGGCTGCGTCTCGCCCGTCGGAGAGCAGGGCGGCGACATCGCGCGTATCGCCGTGCTGAACGCCGACTATGCTGAA
ACCACAGCCGGGGTTCAGGTCGACCGCTTCTGCGCGTCCGGCCTGGAGGCCTGCAATATGGCCGCCTCGAAAGTCATGAC
CGGCGAGGCCGACATGGCCATCGGCGGCGGCGTAGAATCGATGAGCCGCGTGCCGATGGGCGCAGCGGGCGGGGCCTGGT
CTGCTGATCCGCAGATCGCGCTCAAATCCTACTTTACGCCCCAGGGTATCGGCGCAGACACCATCGCCACCAAATACGGC
TTCAGCCGCGATGATGTGGACGCCTTCGCCGTCGAGAGCCAGCGCCGCGCCGCGCAGGCCTGGAAGGAAGGCCGCTTCAA
GAACTCGGTTGTTCCCGTCCGCGACCAGATGGGCGGCATCCGCCTCGCGCATGACGAATTCATGCGCCCGGATACCACCA
TGCAGAGCCTTGCCGCGCTGGACCCGTCCTTCGCGGGCATGGGCGCGATGGGCTTTGATGAAGTCATCAAGCAGCGTTAT
CCCGAGCTTGAGAAGATCAACCACGTCCACCACGCTGGCAACTCCTCGGGCATCGTGGACGGCGCGTCTGCCGTTCTCTT
CGGCTCGAAGGAAATGGGCGAGGCAATGGGCCTCAAACCCCGCGCCCGCGTCCGCGCCATGGCCTCCATCGGCTCGGAGC
CCGGCATCATGCTGACCGGCCCGACCTATGTGACCCAGAAGGTGCTCAAGAAAGCCGGCATGAATGTCGGCGACATCGAC
ATCTACGAACTCAACGAAGCGTTTGCTTCGGTCGTCATGCTGATGATGAAGATGCTCGAAATCCCGCACGAGAAAATGAA
CGTGAATGGCGGCGCCATCGCCATGGGTCACCCCCTCGGCGCAACCGGTGGCATGATCCTTGGTACGGTCCTGGATGAGC
TTGAGCGCTCCGACAAGGAAACCGGCCTCATTACGCTTTGTGTTGGCGCCGGCATGGGCACCGCCACGATCATCGAGCGC
ATCTAG

Upstream 100 bases:

>100_bases
CCGCGGCAAGGAAGCCGCGCGGGCCACCGCCTCTCTCACGCTCAATCCGGCCAAAGCGCCGGCCAAAACACCCAAATCCT
GACCTCCAAAGGAGCCACCC

Downstream 100 bases:

>100_bases
TCACGACACGGAATATTGAGAACACGATCATGAAACTCGAAACATTCAAATTCGACATCGATGCCGATGGCATCGCCCAC
GCTATCTTCGACGTGCCTGG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MPEAYIYDAVRTPRGKGKSSGALHEITALSLGTQVLQAIRDRNNLDTSKVDDVVFGCVSPVGEQGGDIARIAVLNADYAE
TTAGVQVDRFCASGLEACNMAASKVMTGEADMAIGGGVESMSRVPMGAAGGAWSADPQIALKSYFTPQGIGADTIATKYG
FSRDDVDAFAVESQRRAAQAWKEGRFKNSVVPVRDQMGGIRLAHDEFMRPDTTMQSLAALDPSFAGMGAMGFDEVIKQRY
PELEKINHVHHAGNSSGIVDGASAVLFGSKEMGEAMGLKPRARVRAMASIGSEPGIMLTGPTYVTQKVLKKAGMNVGDID
IYELNEAFASVVMLMMKMLEIPHEKMNVNGGAIAMGHPLGATGGMILGTVLDELERSDKETGLITLCVGAGMGTATIIER
I

Sequences:

>Translated_401_residues
MPEAYIYDAVRTPRGKGKSSGALHEITALSLGTQVLQAIRDRNNLDTSKVDDVVFGCVSPVGEQGGDIARIAVLNADYAE
TTAGVQVDRFCASGLEACNMAASKVMTGEADMAIGGGVESMSRVPMGAAGGAWSADPQIALKSYFTPQGIGADTIATKYG
FSRDDVDAFAVESQRRAAQAWKEGRFKNSVVPVRDQMGGIRLAHDEFMRPDTTMQSLAALDPSFAGMGAMGFDEVIKQRY
PELEKINHVHHAGNSSGIVDGASAVLFGSKEMGEAMGLKPRARVRAMASIGSEPGIMLTGPTYVTQKVLKKAGMNVGDID
IYELNEAFASVVMLMMKMLEIPHEKMNVNGGAIAMGHPLGATGGMILGTVLDELERSDKETGLITLCVGAGMGTATIIER
I
>Mature_400_residues
PEAYIYDAVRTPRGKGKSSGALHEITALSLGTQVLQAIRDRNNLDTSKVDDVVFGCVSPVGEQGGDIARIAVLNADYAET
TAGVQVDRFCASGLEACNMAASKVMTGEADMAIGGGVESMSRVPMGAAGGAWSADPQIALKSYFTPQGIGADTIATKYGF
SRDDVDAFAVESQRRAAQAWKEGRFKNSVVPVRDQMGGIRLAHDEFMRPDTTMQSLAALDPSFAGMGAMGFDEVIKQRYP
ELEKINHVHHAGNSSGIVDGASAVLFGSKEMGEAMGLKPRARVRAMASIGSEPGIMLTGPTYVTQKVLKKAGMNVGDIDI
YELNEAFASVVMLMMKMLEIPHEKMNVNGGAIAMGHPLGATGGMILGTVLDELERSDKETGLITLCVGAGMGTATIIERI

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=389, Percent_Identity=36.5038560411311, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=34.0852130325814, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI167614485, Length=409, Percent_Identity=30.0733496332518, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI4557237, Length=411, Percent_Identity=31.6301703163017, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI4504327, Length=441, Percent_Identity=28.7981859410431, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI194353979, Length=124, Percent_Identity=39.5161290322581, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI48994986, Length=408, Percent_Identity=38.4803921568627, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1787663, Length=424, Percent_Identity=37.9716981132075, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082165, Length=416, Percent_Identity=36.0576923076923, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1788554, Length=416, Percent_Identity=37.0192307692308, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788683, Length=430, Percent_Identity=27.2093023255814, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI133906874, Length=416, Percent_Identity=34.375, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17535921, Length=415, Percent_Identity=30.1204819277108, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25147385, Length=418, Percent_Identity=28.9473684210526, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17551802, Length=446, Percent_Identity=26.6816143497758, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=26.980198019802, Blast_Score=111, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322031, Length=358, Percent_Identity=34.3575418994413, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6325229, Length=412, Percent_Identity=30.5825242718447, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24655093, Length=416, Percent_Identity=34.375, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17648125, Length=413, Percent_Identity=34.1404358353511, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24640423, Length=411, Percent_Identity=29.9270072992701, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI17137578, Length=453, Percent_Identity=29.1390728476821, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42359; Mature: 42228

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAYIYDAVRTPRGKGKSSGALHEITALSLGTQVLQAIRDRNNLDTSKVDDVVFGCVSP
CCCCEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VGEQGGDIARIAVLNADYAETTAGVQVDRFCASGLEACNMAASKVMTGEADMAIGGGVES
HCCCCCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
MSRVPMGAAGGAWSADPQIALKSYFTPQGIGADTIATKYGFSRDDVDAFAVESQRRAAQA
HHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
WKEGRFKNSVVPVRDQMGGIRLAHDEFMRPDTTMQSLAALDPSFAGMGAMGFDEVIKQRY
HHHCCCCCCCCCHHHCCCCEEECHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
PELEKINHVHHAGNSSGIVDGASAVLFGSKEMGEAMGLKPRARVRAMASIGSEPGIMLTG
CHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEC
PTYVTQKVLKKAGMNVGDIDIYELNEAFASVVMLMMKMLEIPHEKMNVNGGAIAMGHPLG
CHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCC
ATGGMILGTVLDELERSDKETGLITLCVGAGMGTATIIERI
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
PEAYIYDAVRTPRGKGKSSGALHEITALSLGTQVLQAIRDRNNLDTSKVDDVVFGCVSP
CCCEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VGEQGGDIARIAVLNADYAETTAGVQVDRFCASGLEACNMAASKVMTGEADMAIGGGVES
HCCCCCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
MSRVPMGAAGGAWSADPQIALKSYFTPQGIGADTIATKYGFSRDDVDAFAVESQRRAAQA
HHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
WKEGRFKNSVVPVRDQMGGIRLAHDEFMRPDTTMQSLAALDPSFAGMGAMGFDEVIKQRY
HHHCCCCCCCCCHHHCCCCEEECHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
PELEKINHVHHAGNSSGIVDGASAVLFGSKEMGEAMGLKPRARVRAMASIGSEPGIMLTG
CHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEC
PTYVTQKVLKKAGMNVGDIDIYELNEAFASVVMLMMKMLEIPHEKMNVNGGAIAMGHPLG
CHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCC
ATGGMILGTVLDELERSDKETGLITLCVGAGMGTATIIERI
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]