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The map label for this gene is dapF [H]

Identifier: 114797495

GI number: 114797495

Start: 3663413

End: 3664222

Strand: Reverse

Name: dapF [H]

Synonym: HNE_3529

Alternate gene names: 114797495

Gene position: 3664222-3663413 (Counterclockwise)

Preceding gene: 114798732

Following gene: 114799327

Centisome position: 98.9

GC content: 62.72

Gene sequence:

>810_bases
ATGAAGCTCTGGAAAATGAACGGCGCAGGCAACGCGTTTGCCATATTCGACGCGCGCTCGACGGCCTTCACGCCGACGAC
GGAGCAGATCCGCAAGATCGCTGCAGACCTGAAAGCCGATCAGGTGATTGCGATGGAGCGCGACGCCACCAAGGATGTGT
TCATGCGCATCTGGAATTCCGATGGCTCGGAAGTCTCTGCCTGTGGCAACGCCACCCGCTGCGTCGGCCTGCTGATGCTC
GATGAGACGGGCAAAGACCGCGTCACAATCCAGACAGAGGCCGACATGCTGCGCGCAACCCGCGCCGGCGAAGGGCTGAT
CACCGTGGACATGGGCTCCCCGCAGATGGGTTGGGAAGACATTCCCCTGTCAGAGAAAATGGATGTACGCGGCGTGGACG
TGAAAATCGGCCCGATAGACGCCCCCATCCTCTCCCGGCCTGCTGTGGTCTCGATGGGCAATCCCCATGCGGTGTTCTTC
GTCAAGGATGTTGCTGCCTATGACATCCCGGCCATCGGCCCGCTGGTGGAATGGCACCCGCTGTTTCCCAAGGGCACGAA
TGTCGGTTTTGCCCAGGTGATTGACCGCGAAACCATCCGTCTGCGCGTCTGGGAACGCGCCGCCGGCCTCACCAGGGCCT
GCGGGACAGGCGCCTGCGCGGCCTTGGTCTGCGCCGCCCGCGCCGGGCTCACCGGCCGCAAGGCACGGATGATCCTCGAT
GGCGGTGATCTCACCATCGATTGGCGCGAAAGCGACGATCATGTCTACATGACCGGCCCGGTAGAGCTGGAATTCGAGGC
CGAAATCTGA

Upstream 100 bases:

>100_bases
CATTCAGACGGCGGGCTGGATTGTTTCCGCCCGCGTCCCGAAATAAGGGGCAGGGGCCTTTCGCTTCCCGTGAGAGGGGA
AAGGCATTAGAGAACCGCAG

Downstream 100 bases:

>100_bases
CCCCTTCAGGGGGTTGCCGCTACCCGCGCATCGCCGCACTCTGCCAGTCTTGCTGCTGCCTTACGGAGAGACTGTCTCAT
GCGCCTGCCCCTGCTTCTGA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKLWKMNGAGNAFAIFDARSTAFTPTTEQIRKIAADLKADQVIAMERDATKDVFMRIWNSDGSEVSACGNATRCVGLLML
DETGKDRVTIQTEADMLRATRAGEGLITVDMGSPQMGWEDIPLSEKMDVRGVDVKIGPIDAPILSRPAVVSMGNPHAVFF
VKDVAAYDIPAIGPLVEWHPLFPKGTNVGFAQVIDRETIRLRVWERAAGLTRACGTGACAALVCAARAGLTGRKARMILD
GGDLTIDWRESDDHVYMTGPVELEFEAEI

Sequences:

>Translated_269_residues
MKLWKMNGAGNAFAIFDARSTAFTPTTEQIRKIAADLKADQVIAMERDATKDVFMRIWNSDGSEVSACGNATRCVGLLML
DETGKDRVTIQTEADMLRATRAGEGLITVDMGSPQMGWEDIPLSEKMDVRGVDVKIGPIDAPILSRPAVVSMGNPHAVFF
VKDVAAYDIPAIGPLVEWHPLFPKGTNVGFAQVIDRETIRLRVWERAAGLTRACGTGACAALVCAARAGLTGRKARMILD
GGDLTIDWRESDDHVYMTGPVELEFEAEI
>Mature_269_residues
MKLWKMNGAGNAFAIFDARSTAFTPTTEQIRKIAADLKADQVIAMERDATKDVFMRIWNSDGSEVSACGNATRCVGLLML
DETGKDRVTIQTEADMLRATRAGEGLITVDMGSPQMGWEDIPLSEKMDVRGVDVKIGPIDAPILSRPAVVSMGNPHAVFF
VKDVAAYDIPAIGPLVEWHPLFPKGTNVGFAQVIDRETIRLRVWERAAGLTRACGTGACAALVCAARAGLTGRKARMILD
GGDLTIDWRESDDHVYMTGPVELEFEAEI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=267, Percent_Identity=37.4531835205993, Blast_Score=168, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29228; Mature: 29228

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLWKMNGAGNAFAIFDARSTAFTPTTEQIRKIAADLKADQVIAMERDATKDVFMRIWNS
CEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHEEHEEECC
DGSEVSACGNATRCVGLLMLDETGKDRVTIQTEADMLRATRAGEGLITVDMGSPQMGWED
CCCEEECCCCCCCEEEEEEECCCCCCEEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCC
IPLSEKMDVRGVDVKIGPIDAPILSRPAVVSMGNPHAVFFVKDVAAYDIPAIGPLVEWHP
CCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEEECHHCCCCCCCCCCCCCC
LFPKGTNVGFAQVIDRETIRLRVWERAAGLTRACGTGACAALVCAARAGLTGRKARMILD
CCCCCCCCCHHHHHCCCEEEEEEEHHHCCCHHCCCCHHHHHHHHHHHCCCCCCEEEEEEE
GGDLTIDWRESDDHVYMTGPVELEFEAEI
CCCEEEEEECCCCEEEEECCEEEEEEECC
>Mature Secondary Structure
MKLWKMNGAGNAFAIFDARSTAFTPTTEQIRKIAADLKADQVIAMERDATKDVFMRIWNS
CEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHEEHEEECC
DGSEVSACGNATRCVGLLMLDETGKDRVTIQTEADMLRATRAGEGLITVDMGSPQMGWED
CCCEEECCCCCCCEEEEEEECCCCCCEEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCC
IPLSEKMDVRGVDVKIGPIDAPILSRPAVVSMGNPHAVFFVKDVAAYDIPAIGPLVEWHP
CCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEEECHHCCCCCCCCCCCCCC
LFPKGTNVGFAQVIDRETIRLRVWERAAGLTRACGTGACAALVCAARAGLTGRKARMILD
CCCCCCCCCHHHHHCCCEEEEEEEHHHCCCHHCCCCHHHHHHHHHHHCCCCCCEEEEEEE
GGDLTIDWRESDDHVYMTGPVELEFEAEI
CCCEEEEEECCCCEEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA