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The map label for this gene is rlmN

Identifier: 114797352

GI number: 114797352

Start: 3482686

End: 3483849

Strand: Direct

Name: rlmN

Synonym: HNE_3331

Alternate gene names: 114797352

Gene position: 3482686-3483849 (Clockwise)

Preceding gene: 114799447

Following gene: 114797851

Centisome position: 94.0

GC content: 61.43

Gene sequence:

>1164_bases
ATGACTGTGACCCTGAACCTCAGCCGCCCGAATAGTGCTTTGCCAGGCAAGAAACGCCTGACGGGGCTTTCGGTTCCCGC
GCTCAAGGCAGAAATGGAAGCTTTGGGCCTGGAGCCGAAGGCCGCGAACATGCGCGCCCGCCAGATCCGGCGCTGGATTC
ATCATTTCGGGACCACCGATTTTGCCGCGATGACCGACATCGCCAAGGATTTACGCGCCCAACTCGCCGAGAAATTCGAG
GTCGAGCGCCCCGAAATCGCTGACCATCAGGTCAGCCGGGATGGCACCCAAAAATGGCTGACCCGCTATGGACCCGGCAT
CGAGGGGGAAAGCGTCTATATTCCCGATGTCGGCAAAGCCGGCGCGCTGTGCGTCTCCAGCCAGGTGGGCTGCACCCTCA
ACTGTACCTTCTGCCATACCGGCACGCAGGCGCTTGTCCGCAACCTCACCGCTGCGGAAATTGTCCAGCAGGTCATCATT
GCCCGCGACGCGCTGTCAGAATGGCCAAGCTCCATCGAGGAGCGCCGCCTGACCAACATCGTCTTCATGGGTATGGGTGA
GCCGCTCTATAATCTGGATAATGTCGCCGAGGCGATCGATACGATCTCCGATGGTGATGGCATGGCGATCGGCCGGCGCC
GGATTACCGTCTCGACGGCGGGCGTTGCGCCCAAGATCCCGGAACTGGGCGAACGTACCGGCGCGATGCTCGCCATCTCC
CTGCACGCCACCAATAACGACCTGCGCAACGAACTCGTCCCGCTCAATCGCAAGTACGACATCCAGACGCTGTTCGACGC
CATCCGGGCGTATCCCGGCCTTGGCAATGCGAAGCGGGTGACCTTCGAATATGTCATGCTCAAGGGCATCAATGATACGC
TGGCCGAAGCGCGCGACCTCGTGAAGCTGATGAAAGGCGTGCCCTCCAAGATCAACCTCATTCCGTTCAATCCCTGGCCC
GGCTCGCCCTATGAATGCTCGGACTGGGAAACGATTGAGGAATTTGCCGAAGTGCTGAACCGGGCTGGCTATGCCTCTCC
GATCCGCACGCCGCGCGGCCGCGATATTCTGGCCGCCTGTGGCCAGCTGCGTTCGGAAAGCGTCAAGGTGCGCGCCAGCG
AGCTGCGCAAGCAGCAGGCCTCAGCCGAAGGCGCGGAAAGCTAA

Upstream 100 bases:

>100_bases
TCCCCCCTAAAATGAAGGGATTTTCGAGTTCTGGCGCGTTAAACTCTGTCCATGTTCCCGCCAATTTATTTGTCAGGCGG
CTCGTGCTATAGGCCCGCAC

Downstream 100 bases:

>100_bases
CCTTCCCGCATCAACGTTTCTTGTGAACCAGCCGGTCCCAGATCGATCCGGGGATGTTGGAATAATCATCCGTCCACGCC
CGGCCACCATCGCTCTCCAG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTVTLNLSRPNSALPGKKRLTGLSVPALKAEMEALGLEPKAANMRARQIRRWIHHFGTTDFAAMTDIAKDLRAQLAEKFE
VERPEIADHQVSRDGTQKWLTRYGPGIEGESVYIPDVGKAGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVII
ARDALSEWPSSIEERRLTNIVFMGMGEPLYNLDNVAEAIDTISDGDGMAIGRRRITVSTAGVAPKIPELGERTGAMLAIS
LHATNNDLRNELVPLNRKYDIQTLFDAIRAYPGLGNAKRVTFEYVMLKGINDTLAEARDLVKLMKGVPSKINLIPFNPWP
GSPYECSDWETIEEFAEVLNRAGYASPIRTPRGRDILAACGQLRSESVKVRASELRKQQASAEGAES

Sequences:

>Translated_387_residues
MTVTLNLSRPNSALPGKKRLTGLSVPALKAEMEALGLEPKAANMRARQIRRWIHHFGTTDFAAMTDIAKDLRAQLAEKFE
VERPEIADHQVSRDGTQKWLTRYGPGIEGESVYIPDVGKAGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVII
ARDALSEWPSSIEERRLTNIVFMGMGEPLYNLDNVAEAIDTISDGDGMAIGRRRITVSTAGVAPKIPELGERTGAMLAIS
LHATNNDLRNELVPLNRKYDIQTLFDAIRAYPGLGNAKRVTFEYVMLKGINDTLAEARDLVKLMKGVPSKINLIPFNPWP
GSPYECSDWETIEEFAEVLNRAGYASPIRTPRGRDILAACGQLRSESVKVRASELRKQQASAEGAES
>Mature_386_residues
TVTLNLSRPNSALPGKKRLTGLSVPALKAEMEALGLEPKAANMRARQIRRWIHHFGTTDFAAMTDIAKDLRAQLAEKFEV
ERPEIADHQVSRDGTQKWLTRYGPGIEGESVYIPDVGKAGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVIIA
RDALSEWPSSIEERRLTNIVFMGMGEPLYNLDNVAEAIDTISDGDGMAIGRRRITVSTAGVAPKIPELGERTGAMLAISL
HATNNDLRNELVPLNRKYDIQTLFDAIRAYPGLGNAKRVTFEYVMLKGINDTLAEARDLVKLMKGVPSKINLIPFNPWPG
SPYECSDWETIEEFAEVLNRAGYASPIRTPRGRDILAACGQLRSESVKVRASELRKQQASAEGAES

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=365, Percent_Identity=43.5616438356164, Blast_Score=294, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_HYPNA (Q0BWY9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762004.1
- ProteinModelPortal:   Q0BWY9
- STRING:   Q0BWY9
- GeneID:   4287206
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3331
- NMPDR:   fig|228405.5.peg.3190
- TIGR:   HNE_3331
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q0BWY9
- ProtClustDB:   CLSK2780964
- BioCyc:   HNEP81032:HNE_3331-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42461; Mature: 42330

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTLNLSRPNSALPGKKRLTGLSVPALKAEMEALGLEPKAANMRARQIRRWIHHFGTTD
CEEEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCH
FAAMTDIAKDLRAQLAEKFEVERPEIADHQVSRDGTQKWLTRYGPGIEGESVYIPDVGKA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCC
GALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVIIARDALSEWPSSIEERRLTNI
CCEEEECCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VFMGMGEPLYNLDNVAEAIDTISDGDGMAIGRRRITVSTAGVAPKIPELGERTGAMLAIS
EEEECCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCCHHHCCCCCEEEEE
LHATNNDLRNELVPLNRKYDIQTLFDAIRAYPGLGNAKRVTFEYVMLKGINDTLAEARDL
EECCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHEECCCCHHHHHHHHH
VKLMKGVPSKINLIPFNPWPGSPYECSDWETIEEFAEVLNRAGYASPIRTPRGRDILAAC
HHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
GQLRSESVKVRASELRKQQASAEGAES
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVTLNLSRPNSALPGKKRLTGLSVPALKAEMEALGLEPKAANMRARQIRRWIHHFGTTD
EEEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCH
FAAMTDIAKDLRAQLAEKFEVERPEIADHQVSRDGTQKWLTRYGPGIEGESVYIPDVGKA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCC
GALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVIIARDALSEWPSSIEERRLTNI
CCEEEECCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VFMGMGEPLYNLDNVAEAIDTISDGDGMAIGRRRITVSTAGVAPKIPELGERTGAMLAIS
EEEECCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCCHHHCCCCCEEEEE
LHATNNDLRNELVPLNRKYDIQTLFDAIRAYPGLGNAKRVTFEYVMLKGINDTLAEARDL
EECCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHEECCCCHHHHHHHHH
VKLMKGVPSKINLIPFNPWPGSPYECSDWETIEEFAEVLNRAGYASPIRTPRGRDILAAC
HHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
GQLRSESVKVRASELRKQQASAEGAES
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA