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The map label for this gene is mltB [H]

Identifier: 114332428

GI number: 114332428

Start: 2604805

End: 2605878

Strand: Reverse

Name: mltB [H]

Synonym: Neut_2477

Alternate gene names: 114332428

Gene position: 2605878-2604805 (Counterclockwise)

Preceding gene: 114332431

Following gene: 114332421

Centisome position: 97.93

GC content: 44.04

Gene sequence:

>1074_bases
ATGAACTACGATTTCTGCTCAACTCGTCCGGCATTCCTGAACTTCTTGGTTAAAAATCTATTCAGGCTGATAAATTCCAG
TTATTTTCCGGATCGCCTGCTTTTATTTATGCCAATAATCCTGATTGTTTATTCTTCAGTTTGTCTAGCCGCACAACCAA
CTCCCATTTCGCGCTCAGAAGTCAGAAATTTTATAACGTATATGGTGAACCACCATCAATATGAAATAGACGAATTAGAA
AAAATATTTTCTCAGACAAATTTCCGGCCCGATATTCTCAAGCTGATCTCTACCCCGGCCTCAGCAATTACCTGGGATGA
ATATCGGAAACGATTTGTCAATATGCAGCGCATTAGAGGTGGAATTAATTTCTGGAACAAGCACGCCAGCGAATTGGAAC
AGGCAAGAAAAATCTACGGTATTCCTGAGGAAATCATCGTCGCGATTATCGGTATTGAGACTGCTTATGGCTCATCAACC
GGCACCTACCGCATCATGGATGCATTGACAACACTAGCTTTCGATTTCCCCAGAAGAGCAGACTATTTTCGTGAAGAGCT
GGAGCAGTATTTATTGCTGGCAAGGGAGCAAAAATTTGGTTTGCTGGACATCAAAGGCTCTTATGCCGGGGCAATTGGCA
TCCCTCAATTTATGCCCGGAAGCTACAGGCGCTACGCAGTTGATTTTAATCATGATGGGAAAATTGATTTATCCGGGAGT
GCTGCCGATGCGATTGGCAGTATAGGCAATTATCTGAAGGAATATGGATGGGAAGCAGGCAAGCCGATTGCTGTTCGTGC
GCGGATCAGATCAGAAAACTTTCAGAAGTTTCTGGATGCGGGCATAGAACCGTTACATTCAGTTAAAAAATTACGCGAAG
CCGGCATCATCCCACTCGATTCAATTACAGATGACACTCTGTCAGCCTTGCTGGAATTGAACAGCCAAGATAACCGCCAG
TTCTGGCTGGGATTCAGAAATTTTTATGCAATCACACGCTACAACCGCAGCACTTTTTATGCCATGTCCGTTTTTGAGCT
GGCCAGAGCAATTCGCGTTGCCAGATCTTCATAG

Upstream 100 bases:

>100_bases
AAGCTGACAACTTTCAATGAAACACCTCTCTTTACCTGATCAAAGCAAATACTTCGAACTGATTCGCAGCACTCTGAAAC
ATTTATCAGGATTTTTACAA

Downstream 100 bases:

>100_bases
CCAGCAGGTTGATAACCATCAAATAGACCCTTCGAGGCGGTGCCCAAGATGATAGTCTCACTGGCGACGATTAATCGTCC
TGTAAGCGTACCGCAAGCAC

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNYDFCSTRPAFLNFLVKNLFRLINSSYFPDRLLLFMPIILIVYSSVCLAAQPTPISRSEVRNFITYMVNHHQYEIDELE
KIFSQTNFRPDILKLISTPASAITWDEYRKRFVNMQRIRGGINFWNKHASELEQARKIYGIPEEIIVAIIGIETAYGSST
GTYRIMDALTTLAFDFPRRADYFREELEQYLLLAREQKFGLLDIKGSYAGAIGIPQFMPGSYRRYAVDFNHDGKIDLSGS
AADAIGSIGNYLKEYGWEAGKPIAVRARIRSENFQKFLDAGIEPLHSVKKLREAGIIPLDSITDDTLSALLELNSQDNRQ
FWLGFRNFYAITRYNRSTFYAMSVFELARAIRVARSS

Sequences:

>Translated_357_residues
MNYDFCSTRPAFLNFLVKNLFRLINSSYFPDRLLLFMPIILIVYSSVCLAAQPTPISRSEVRNFITYMVNHHQYEIDELE
KIFSQTNFRPDILKLISTPASAITWDEYRKRFVNMQRIRGGINFWNKHASELEQARKIYGIPEEIIVAIIGIETAYGSST
GTYRIMDALTTLAFDFPRRADYFREELEQYLLLAREQKFGLLDIKGSYAGAIGIPQFMPGSYRRYAVDFNHDGKIDLSGS
AADAIGSIGNYLKEYGWEAGKPIAVRARIRSENFQKFLDAGIEPLHSVKKLREAGIIPLDSITDDTLSALLELNSQDNRQ
FWLGFRNFYAITRYNRSTFYAMSVFELARAIRVARSS
>Mature_357_residues
MNYDFCSTRPAFLNFLVKNLFRLINSSYFPDRLLLFMPIILIVYSSVCLAAQPTPISRSEVRNFITYMVNHHQYEIDELE
KIFSQTNFRPDILKLISTPASAITWDEYRKRFVNMQRIRGGINFWNKHASELEQARKIYGIPEEIIVAIIGIETAYGSST
GTYRIMDALTTLAFDFPRRADYFREELEQYLLLAREQKFGLLDIKGSYAGAIGIPQFMPGSYRRYAVDFNHDGKIDLSGS
AADAIGSIGNYLKEYGWEAGKPIAVRARIRSENFQKFLDAGIEPLHSVKKLREAGIIPLDSITDDTLSALLELNSQDNRQ
FWLGFRNFYAITRYNRSTFYAMSVFELARAIRVARSS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=306, Percent_Identity=41.5032679738562, Blast_Score=254, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41048; Mature: 41048

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDFCSTRPAFLNFLVKNLFRLINSSYFPDRLLLFMPIILIVYSSVCLAAQPTPISRSE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VRNFITYMVNHHQYEIDELEKIFSQTNFRPDILKLISTPASAITWDEYRKRFVNMQRIRG
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
GINFWNKHASELEQARKIYGIPEEIIVAIIGIETAYGSSTGTYRIMDALTTLAFDFPRRA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHH
DYFREELEQYLLLAREQKFGLLDIKGSYAGAIGIPQFMPGSYRRYAVDFNHDGKIDLSGS
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCC
AADAIGSIGNYLKEYGWEAGKPIAVRARIRSENFQKFLDAGIEPLHSVKKLREAGIIPLD
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
SITDDTLSALLELNSQDNRQFWLGFRNFYAITRYNRSTFYAMSVFELARAIRVARSS
CCCHHHHHHHHHCCCCCCCEEEEEHHHHEEEEEECCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYDFCSTRPAFLNFLVKNLFRLINSSYFPDRLLLFMPIILIVYSSVCLAAQPTPISRSE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VRNFITYMVNHHQYEIDELEKIFSQTNFRPDILKLISTPASAITWDEYRKRFVNMQRIRG
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
GINFWNKHASELEQARKIYGIPEEIIVAIIGIETAYGSSTGTYRIMDALTTLAFDFPRRA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHH
DYFREELEQYLLLAREQKFGLLDIKGSYAGAIGIPQFMPGSYRRYAVDFNHDGKIDLSGS
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCC
AADAIGSIGNYLKEYGWEAGKPIAVRARIRSENFQKFLDAGIEPLHSVKKLREAGIIPLD
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
SITDDTLSALLELNSQDNRQFWLGFRNFYAITRYNRSTFYAMSVFELARAIRVARSS
CCCHHHHHHHHHCCCCCCCEEEEEHHHHEEEEEECCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]