Click here to switch to the map view.

The map label for this gene is rmlA1 [C]

Identifier: 114332331

GI number: 114332331

Start: 2491112

End: 2491837

Strand: Reverse

Name: rmlA1 [C]

Synonym: Neut_2374

Alternate gene names: 114332331

Gene position: 2491837-2491112 (Counterclockwise)

Preceding gene: 114332332

Following gene: 114332330

Centisome position: 93.64

GC content: 48.9

Gene sequence:

>726_bases
ATGTTCAAAATCATGATCCTGGCAGCTGGCAAAGGGGAACGCATGAAGCCGCTCACTGATACCTGCCCCAAACCATTGCT
GCGGGCGGGTGGAAAAATGCTGATCGAATATCATCTGGAGAAACTGGTACAGGCGGGTTTTTCCAGCGTTGTCATTAATC
ACGCCTATCTTGGCGACATGATTGAAGCTGCACTGCAGGATGGCAAACGCTATGGCCTCCATATCCAATATTCACCGGAA
TCCATCGTACTGGAAACCGCAGGCGGAATTACCAATGCACTACCACTGCTCACCAACCCCGATAAACACCAGCCTTTTGC
AGTTATCAACGCTGATATTTTCTGCGATATGGATTTCTCCACCCTGTGGCCGATTTTGCAACAGATGCAATCAAATTCAG
AGCAAATATTGGCTCACCTGATCCTGGTAAATAACCCGCCACATCATCCAGAAGGTGATTTTTCGCTACATAACGGTTCC
GACAAACTGGTTGAATCAACTTGTCCGGATTGCCGAAAACTCACTTTCAGCGGCATCGGGGTTTATCATCCCGCCCTTTT
CAAAAATGTGCTCCCCAATCAAGCTGAAAAACTGGCTCCCCTGCTGCGCCAGGCTATTACTGCCGGAAAAGTTACCGGCT
CACATTACTTAGGTATATGGATGGACATCGGCACCCCTAAACGCCTGCAACTGCTCGATACCAAACTCAGGCAATCTGCG
CTGTGA

Upstream 100 bases:

>100_bases
GCGAACGCTATGGCGAGCTTCATCCGCTGGCCCGACTGCTGGATCAACTGGAAGAAAAAACACCTGCAACCGGTTATACT
TTCTGACCGAGAAACACCTG

Downstream 100 bases:

>100_bases
ACCTGTTTATGGTCTTCTGGGCAATATGTCAGGTGGATAAATTGCAGGGCTGGTATTGAGCAGTTGCAGTTGTATCTGGG
TGAGTATTTGTTATCAAGGG

Product: nucleotidyl transferase

Products: dTDPglucose; Diphosphate [C]

Alternate protein names: Nucleotidyltransferase Family Protein; Nucleoside-Diphosphate-Sugar Pyrophosphorylase; Mannose-1-Phosphate Guanyltransferase; Nucleotidyl Transferase Family Protein; Nucleotidyltransferase; Mannose-1-Phosphate Guanylyltransferase; Nucleotidyl Transferase Superfamily; Sugar-Phosphate Nucleotidyl Transferase; Sugar-Phosphate Nucleotide Transferase; Mannose-1-Phosphate Guanyltransferase-Related Protein; Nucleotidyltransferase Protein; Nucleotidyl Transferase Protein; Transaldolase AB; ADP-Glucose Pyrophosphorylase; Mannose-1-Phosphate Guanyltransferase Protein; Two-Component Fusion Protein; Nucleotidyl Transferase Superfamily Protein; Sugar Phosphate Nucleotidyltransferase; Sugar-Phosphate Nucleotidyltransferase; Polysaccharide Biosynthesis Protein; Glucose-1-Phosphate Nucleotidyltransferase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MFKIMILAAGKGERMKPLTDTCPKPLLRAGGKMLIEYHLEKLVQAGFSSVVINHAYLGDMIEAALQDGKRYGLHIQYSPE
SIVLETAGGITNALPLLTNPDKHQPFAVINADIFCDMDFSTLWPILQQMQSNSEQILAHLILVNNPPHHPEGDFSLHNGS
DKLVESTCPDCRKLTFSGIGVYHPALFKNVLPNQAEKLAPLLRQAITAGKVTGSHYLGIWMDIGTPKRLQLLDTKLRQSA
L

Sequences:

>Translated_241_residues
MFKIMILAAGKGERMKPLTDTCPKPLLRAGGKMLIEYHLEKLVQAGFSSVVINHAYLGDMIEAALQDGKRYGLHIQYSPE
SIVLETAGGITNALPLLTNPDKHQPFAVINADIFCDMDFSTLWPILQQMQSNSEQILAHLILVNNPPHHPEGDFSLHNGS
DKLVESTCPDCRKLTFSGIGVYHPALFKNVLPNQAEKLAPLLRQAITAGKVTGSHYLGIWMDIGTPKRLQLLDTKLRQSA
L
>Mature_241_residues
MFKIMILAAGKGERMKPLTDTCPKPLLRAGGKMLIEYHLEKLVQAGFSSVVINHAYLGDMIEAALQDGKRYGLHIQYSPE
SIVLETAGGITNALPLLTNPDKHQPFAVINADIFCDMDFSTLWPILQQMQSNSEQILAHLILVNNPPHHPEGDFSLHNGS
DKLVESTCPDCRKLTFSGIGVYHPALFKNVLPNQAEKLAPLLRQAITAGKVTGSHYLGIWMDIGTPKRLQLLDTKLRQSA
L

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11761621, Length=231, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI11761619, Length=231, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=229, Percent_Identity=25.764192139738, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=232, Percent_Identity=28.8793103448276, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355443, Length=228, Percent_Identity=26.7543859649123, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24644084, Length=228, Percent_Identity=26.7543859649123, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.24 [C]

Molecular weight: Translated: 26602; Mature: 26602

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIMILAAGKGERMKPLTDTCPKPLLRAGGKMLIEYHLEKLVQAGFSSVVINHAYLGDM
CEEEEEEECCCCCCCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IEAALQDGKRYGLHIQYSPESIVLETAGGITNALPLLTNPDKHQPFAVINADIFCDMDFS
HHHHHHCCCCCCEEEEECCCEEEEECCCCCHHCCCCCCCCCCCCCEEEEECEEEEECCHH
TLWPILQQMQSNSEQILAHLILVNNPPHHPEGDFSLHNGSDKLVESTCPDCRKLTFSGIG
HHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCEEECCCHHHHHHCCCHHHHHHHCCCC
VYHPALFKNVLPNQAEKLAPLLRQAITAGKVTGSHYLGIWMDIGTPKRLQLLDTKLRQSA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure
MFKIMILAAGKGERMKPLTDTCPKPLLRAGGKMLIEYHLEKLVQAGFSSVVINHAYLGDM
CEEEEEEECCCCCCCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IEAALQDGKRYGLHIQYSPESIVLETAGGITNALPLLTNPDKHQPFAVINADIFCDMDFS
HHHHHHCCCCCCEEEEECCCEEEEECCCCCHHCCCCCCCCCCCCCEEEEECEEEEECCHH
TLWPILQQMQSNSEQILAHLILVNNPPHHPEGDFSLHNGSDKLVESTCPDCRKLTFSGIG
HHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCEEECCCHHHHHHCCCHHHHHHHCCCC
VYHPALFKNVLPNQAEKLAPLLRQAITAGKVTGSHYLGIWMDIGTPKRLQLLDTKLRQSA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; D-Glucose 1-phosphate; H+ [C]

Specific reaction: dTTP + D-Glucose 1-phosphate + H+ --> dTDPglucose + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA