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The map label for this gene is nudF [H]

Identifier: 114332327

GI number: 114332327

Start: 2487012

End: 2487647

Strand: Reverse

Name: nudF [H]

Synonym: Neut_2370

Alternate gene names: 114332327

Gene position: 2487647-2487012 (Counterclockwise)

Preceding gene: 114332328

Following gene: 114332326

Centisome position: 93.48

GC content: 47.17

Gene sequence:

>636_bases
TTGCTGGTAATAAACTACTCAAACATCCATAAAATGGATTTCGAAATACTGGAAAAAACCATCTGCTTTCAGGGTTTTTT
CCGTCTAGAGCAGTACCGCCTGCGTCACCGTAAATTCGACGGAGAGTGGGGGCGCCCGATCACACGTGAATTATTTGAGC
GGGGACATGCAGCAGCAGTATTGCCTTACGATCCGCACACTGATGAAATTTTACTGATTGAACAGTTTCGTGCAGGCGCC
ATGTCTGCACCAGATGGTCCTTGGTTGCTGGAAATTGTGGCGGGCATAATTGAAGCTGATGAAGCGCCAGAACAGGTGGT
TGCAAGAGAATCCATCGAGGAAGCGGATTGTCAGATCAGTAGCCTGATCCCGCTGTATGATTATCTGGTCAGCCCCGGTG
GTACGACTGAGCGAATTGTATTGTTCTGTGGTCGGGTTGATATGAAAACGATTGATGCGGGCAGTATTCATGGCAATGGC
GAAGAAGATGAGGATATCAAGGTGCATGTCATGTCTTTTCATGATGCGTTGGAACTATTGAATACCGGTCAGATTAATTC
TGCCAGTGCTATTATTGCATTACAGTGGCTTGCTTTGAACCGCGACACTGTTCAGCAGCACTGGCTGTCAAGATAG

Upstream 100 bases:

>100_bases
TTATCTGCCGTGTCAGGACTTGATAGCCAGAACACGTCCGTTAAGTACCTGAATGAAATCTTGATGCTATCTCTGCCAGC
AATTGGGAATAAATCACTGA

Downstream 100 bases:

>100_bases
GAGTGCACGCATGTGTGCCAGTAAATCTACAGGATTTTACCAGGCACAATATAGTTTGCTGCTGGCTATTTTTCTATGGG
CTGGCATGCTGTTTGCACAA

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MLVINYSNIHKMDFEILEKTICFQGFFRLEQYRLRHRKFDGEWGRPITRELFERGHAAAVLPYDPHTDEILLIEQFRAGA
MSAPDGPWLLEIVAGIIEADEAPEQVVARESIEEADCQISSLIPLYDYLVSPGGTTERIVLFCGRVDMKTIDAGSIHGNG
EEDEDIKVHVMSFHDALELLNTGQINSASAIIALQWLALNRDTVQQHWLSR

Sequences:

>Translated_211_residues
MLVINYSNIHKMDFEILEKTICFQGFFRLEQYRLRHRKFDGEWGRPITRELFERGHAAAVLPYDPHTDEILLIEQFRAGA
MSAPDGPWLLEIVAGIIEADEAPEQVVARESIEEADCQISSLIPLYDYLVSPGGTTERIVLFCGRVDMKTIDAGSIHGNG
EEDEDIKVHVMSFHDALELLNTGQINSASAIIALQWLALNRDTVQQHWLSR
>Mature_211_residues
MLVINYSNIHKMDFEILEKTICFQGFFRLEQYRLRHRKFDGEWGRPITRELFERGHAAAVLPYDPHTDEILLIEQFRAGA
MSAPDGPWLLEIVAGIIEADEAPEQVVARESIEEADCQISSLIPLYDYLVSPGGTTERIVLFCGRVDMKTIDAGSIHGNG
EEDEDIKVHVMSFHDALELLNTGQINSASAIIALQWLALNRDTVQQHWLSR

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=198, Percent_Identity=46.4646464646465, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1788810, Length=184, Percent_Identity=32.0652173913043, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23950; Mature: 23950

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVINYSNIHKMDFEILEKTICFQGFFRLEQYRLRHRKFDGEWGRPITRELFERGHAAAV
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEE
LPYDPHTDEILLIEQFRAGAMSAPDGPWLLEIVAGIIEADEAPEQVVARESIEEADCQIS
ECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHH
SLIPLYDYLVSPGGTTERIVLFCGRVDMKTIDAGSIHGNGEEDEDIKVHVMSFHDALELL
HHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHH
NTGQINSASAIIALQWLALNRDTVQQHWLSR
HCCCCCCCHHEEEEHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MLVINYSNIHKMDFEILEKTICFQGFFRLEQYRLRHRKFDGEWGRPITRELFERGHAAAV
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEE
LPYDPHTDEILLIEQFRAGAMSAPDGPWLLEIVAGIIEADEAPEQVVARESIEEADCQIS
ECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHH
SLIPLYDYLVSPGGTTERIVLFCGRVDMKTIDAGSIHGNGEEDEDIKVHVMSFHDALELL
HHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHH
NTGQINSASAIIALQWLALNRDTVQQHWLSR
HCCCCCCCHHEEEEHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]