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The map label for this gene is mutY [H]

Identifier: 114332269

GI number: 114332269

Start: 2433192

End: 2434322

Strand: Reverse

Name: mutY [H]

Synonym: Neut_2309

Alternate gene names: 114332269

Gene position: 2434322-2433192 (Counterclockwise)

Preceding gene: 114332270

Following gene: 114332268

Centisome position: 91.48

GC content: 46.15

Gene sequence:

>1131_bases
ATGATTCAACAGGCAACCAGACGGCCTCGGACTTCAGCTGTTGAATCAGATTTTTTCGCTGGCAGGTTGATCCGCTGGCA
GCTTGTATACGGGCGACATTCCCTGCCATGGCAGGGAATCAGTGATCCTTATGCTATATGGATATCCGAGATAATGCTGC
AACAAACACAGGTCAGCACGGTTATTCCATATTACGAGCGTTTTATGACTGTTTTTCCAAATATTGCACCACTTGCCAGC
GCGCCGGTTGAAGAAGTGCTCACGCTATGGAGCGGTTTGGGTTATTACTCACGCGGACGCAATTTGCACCGGACAGCACG
TATGATAATGGAGCAGTATGGTGGCGCTTTTCCGCAGGATATCGCTACACTTCAACGATTGCCAGGGATAGGCCGTTCTA
CCGCAGCGGCCATTGCTGCTTTTGCATTTGGTAAGCGTTGCACGATTCTTGACGGAAACGTCAAGCGTATTTTGATCCGT
TACTTCGGTGTTAACGGGCACCCAGGTGAAAGAATGATTGAAGAACAACTGTGGCAATTGGCGGAAGGTCTGTTGCCTGT
TGAAGAAGATCATAAAACTATAGCATCCTATACCCAGGCACTAATGGATCTTGGAGCGCTGGTCTGTGTGCGTACCCAAC
CACGTTGCGAACATTGTCCGTTACAGGCTGATTGCTACGCATATCAGAATGATCTGACGGCCGTTTTGCCTGCTCCCAAG
CCCAGGAAAACGCCTCCTGTCAAAGAAATTGTACATTTAATCCTCCTGAATCAAGGCCGTATTTTGCTAAAGAAGCGACC
AGCTTCCGGTATTTGGGGTGGATTGTGGTGTTTCCCGGAAACTTCTATTGATGAGGACAGTGTTGATTACTGTAAAAAGA
ATTTACATCTACAGGTGTTAAAATTTGCTGTGCTTCCTCATTTGCAACATACGTTCACACATTTTAAATTGATTATTCAG
CCGCAACTCCTGCAATCAATAATGTATCTGCCCGAATGTGAAGAAAATAGCGAGGAAAATGACTTTCTTTGGATGACAAT
TGAGGAAGCTTTGCAACGGGCAATCCCCGCTCCTGTCAGAAAATTGTTGTTAATGACCCAACCCTTACTCCAATCTTGTG
TTCATGAGTAA

Upstream 100 bases:

>100_bases
GCTTATGTTTGTCTTCATTATCCTGCAGGCTCTTCTGTTGGGAAAATACATGGAGAAAAATGATAACCAGAAAATATAAT
TATGATGTACCGTTAAATTT

Downstream 100 bases:

>100_bases
CGTAACTTCCCAGCACTGGAAACAGCAGCAGCGTACTCATCTGCGCGAATTACGCCTGCAGATCCCAGCAGCACAGCGCG
TTCTCTGGAGTGATGCAATT

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIQQATRRPRTSAVESDFFAGRLIRWQLVYGRHSLPWQGISDPYAIWISEIMLQQTQVSTVIPYYERFMTVFPNIAPLAS
APVEEVLTLWSGLGYYSRGRNLHRTARMIMEQYGGAFPQDIATLQRLPGIGRSTAAAIAAFAFGKRCTILDGNVKRILIR
YFGVNGHPGERMIEEQLWQLAEGLLPVEEDHKTIASYTQALMDLGALVCVRTQPRCEHCPLQADCYAYQNDLTAVLPAPK
PRKTPPVKEIVHLILLNQGRILLKKRPASGIWGGLWCFPETSIDEDSVDYCKKNLHLQVLKFAVLPHLQHTFTHFKLIIQ
PQLLQSIMYLPECEENSEENDFLWMTIEEALQRAIPAPVRKLLLMTQPLLQSCVHE

Sequences:

>Translated_376_residues
MIQQATRRPRTSAVESDFFAGRLIRWQLVYGRHSLPWQGISDPYAIWISEIMLQQTQVSTVIPYYERFMTVFPNIAPLAS
APVEEVLTLWSGLGYYSRGRNLHRTARMIMEQYGGAFPQDIATLQRLPGIGRSTAAAIAAFAFGKRCTILDGNVKRILIR
YFGVNGHPGERMIEEQLWQLAEGLLPVEEDHKTIASYTQALMDLGALVCVRTQPRCEHCPLQADCYAYQNDLTAVLPAPK
PRKTPPVKEIVHLILLNQGRILLKKRPASGIWGGLWCFPETSIDEDSVDYCKKNLHLQVLKFAVLPHLQHTFTHFKLIIQ
PQLLQSIMYLPECEENSEENDFLWMTIEEALQRAIPAPVRKLLLMTQPLLQSCVHE
>Mature_376_residues
MIQQATRRPRTSAVESDFFAGRLIRWQLVYGRHSLPWQGISDPYAIWISEIMLQQTQVSTVIPYYERFMTVFPNIAPLAS
APVEEVLTLWSGLGYYSRGRNLHRTARMIMEQYGGAFPQDIATLQRLPGIGRSTAAAIAAFAFGKRCTILDGNVKRILIR
YFGVNGHPGERMIEEQLWQLAEGLLPVEEDHKTIASYTQALMDLGALVCVRTQPRCEHCPLQADCYAYQNDLTAVLPAPK
PRKTPPVKEIVHLILLNQGRILLKKRPASGIWGGLWCFPETSIDEDSVDYCKKNLHLQVLKFAVLPHLQHTFTHFKLIIQ
PQLLQSIMYLPECEENSEENDFLWMTIEEALQRAIPAPVRKLLLMTQPLLQSCVHE

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI6912520, Length=371, Percent_Identity=36.1185983827493, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI115298648, Length=371, Percent_Identity=36.1185983827493, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI115298650, Length=371, Percent_Identity=36.1185983827493, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI190358497, Length=371, Percent_Identity=36.1185983827493, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI115298654, Length=371, Percent_Identity=36.1185983827493, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI115298652, Length=371, Percent_Identity=36.1185983827493, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI1789331, Length=352, Percent_Identity=42.6136363636364, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1787920, Length=188, Percent_Identity=27.6595744680851, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 42833; Mature: 42833

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQQATRRPRTSAVESDFFAGRLIRWQLVYGRHSLPWQGISDPYAIWISEIMLQQTQVST
CCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VIPYYERFMTVFPNIAPLASAPVEEVLTLWSGLGYYSRGRNLHRTARMIMEQYGGAFPQD
HHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCHHH
IATLQRLPGIGRSTAAAIAAFAFGKRCTILDGNVKRILIRYFGVNGHPGERMIEEQLWQL
HHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AEGLLPVEEDHKTIASYTQALMDLGALVCVRTQPRCEHCPLQADCYAYQNDLTAVLPAPK
HHCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHCCCEEECCCCC
PRKTPPVKEIVHLILLNQGRILLKKRPASGIWGGLWCFPETSIDEDSVDYCKKNLHLQVL
CCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHCCHHHHH
KFAVLPHLQHTFTHFKLIIQPQLLQSIMYLPECEENSEENDFLWMTIEEALQRAIPAPVR
HHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHCCHHHH
KLLLMTQPLLQSCVHE
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIQQATRRPRTSAVESDFFAGRLIRWQLVYGRHSLPWQGISDPYAIWISEIMLQQTQVST
CCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VIPYYERFMTVFPNIAPLASAPVEEVLTLWSGLGYYSRGRNLHRTARMIMEQYGGAFPQD
HHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCHHH
IATLQRLPGIGRSTAAAIAAFAFGKRCTILDGNVKRILIRYFGVNGHPGERMIEEQLWQL
HHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AEGLLPVEEDHKTIASYTQALMDLGALVCVRTQPRCEHCPLQADCYAYQNDLTAVLPAPK
HHCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHCCCEEECCCCC
PRKTPPVKEIVHLILLNQGRILLKKRPASGIWGGLWCFPETSIDEDSVDYCKKNLHLQVL
CCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHCCHHHHH
KFAVLPHLQHTFTHFKLIIQPQLLQSIMYLPECEENSEENDFLWMTIEEALQRAIPAPVR
HHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHCCHHHH
KLLLMTQPLLQSCVHE
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]