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The map label for this gene is rpoN [H]

Identifier: 114332263

GI number: 114332263

Start: 2428067

End: 2429530

Strand: Direct

Name: rpoN [H]

Synonym: Neut_2303

Alternate gene names: 114332263

Gene position: 2428067-2429530 (Clockwise)

Preceding gene: 114332262

Following gene: 114332264

Centisome position: 91.24

GC content: 45.97

Gene sequence:

>1464_bases
ATGAAGCCGACTCTGCAACTCAAGCTTTCTACCGGCCTTACACTGACACCGCAACTGAAACAATCAATTCAGCTGCTTCA
ACTATCTGCACTTGAACTCAACCAGGAAATCTCACGTATTGTCCAAGAAAACCCTTTGCTTGAGCTGGATGAAGATATCG
ATCAAGAAAAACTTGAAACTGAAGGACAAGATTCTTCGTTAGTATCATCAGATTTATCTACTACAGCAGAAGCTAATGAA
TCCCATGATATTAGAGAAAACACTGAAATATCGAACGACTGGCTAGATGACTCATTGTATTCAGATCAGGGGGCTTTTTT
AGACACACGTAGACAAGATGATGAGGAGTATGATCAGGACTATTCTCGCCTGGTTAGCAAACCCGCCAGCTTGCGTGAAC
ACCTGCTCATGCAAATCAGCTTGAGGCAACGTTGCGAACGAAAAAAGAAAATTACCGCGCTGCTGGTGGATAGTCTGGAC
GATAATGGTTATTTTACTCAGGATCTGGAAGAGCTTGCCAAGTTACTTCCGGTTGAGCTGGAAATCGACATCACGGACCT
GAAGTCAGCCCTTGCTTATATTCAGCAACTGGATCCGCCCGGCGTAGGAGCACGCAATCTGCGGGAGTGTCTGTCTATAC
AGCTTTCAGCATTGCCGGATGCAACGCCACTACGAACGGAAGCACTCAAACTGGTCAATGAGCACCTGGAAAGTGTCGCT
GCCAAAAATTTCCCATTGCTCAGGAAAGTTCTTGGCTGTGATGAAACCTGTTTACAGTCAGTTTACCAGTTGATCACTCA
ACTCAATCCCAAGCCTGGCGATGATTTCAACGCAACAACAGCACGTTATGTCATTCCTGATGTGATTGTGACCAAATCGG
CTAAAGGCTGGGTGGCACAGCTCAATTCCGATTCAGTTCCGCGTGTGCGCGTTAACCATTTATATGCCGACATTCTGAAG
CAGAATCGTAATGAAGCAATCCGACCACTGATGGAGCAACTGAAAGAGGCCCGCTGGCTGATCAGAAATATCGACCAGCG
CATGGTTACCATACTCCGGGCCAGTCAGGCGATTATTGATTGCCAGCAAGCATTCCTGGAGCATGGCGAAACAGCTGTGC
GCCCCCTTGTTATGCGTGAAATTGCCGAAGTTCTTGGATTACATGAGTCAACCATTTCTCGTATCACAACCCAGAAGTAT
ATGCGGACACCGCACGGTATCTTTGAGCTCAAATATTTCTTTGGAAGCCATATTCCCGCTGAAACGGGTGAAGCACATTC
AGCCATAGCTATTCGTGGACTTATTAAGCAATTGATCCAGAATGAGGATCGAAAAAAGCCACTTAATGATAGTCAAGTTT
CACAAATACTCGCGCAGCAGGGTATTGTTGTTGCTCGCCGTACTGTTGCAAAATATCGGGAGTTCATGCATATTCCCCCG
ACTAATTTACGTAAAATACCCTAG

Upstream 100 bases:

>100_bases
GCGAAGGAACCGTGCTTGCCAGTGGAGTCCCTGCTGAAATCATCAATGACGGACGTGTCCGGGAAGTTTATCTGGGGGAG
CATTTCAGGCTCTGAAAAAT

Downstream 100 bases:

>100_bases
GAAAATTCTGAAAAAATCAGCTGATTACCATGAAGCAAATCAAGGTGAAAAAGTACAGTTCACAAATAGCATATGTGTAC
TCGAAACCTGATTTCAGGTC

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKPTLQLKLSTGLTLTPQLKQSIQLLQLSALELNQEISRIVQENPLLELDEDIDQEKLETEGQDSSLVSSDLSTTAEANE
SHDIRENTEISNDWLDDSLYSDQGAFLDTRRQDDEEYDQDYSRLVSKPASLREHLLMQISLRQRCERKKKITALLVDSLD
DNGYFTQDLEELAKLLPVELEIDITDLKSALAYIQQLDPPGVGARNLRECLSIQLSALPDATPLRTEALKLVNEHLESVA
AKNFPLLRKVLGCDETCLQSVYQLITQLNPKPGDDFNATTARYVIPDVIVTKSAKGWVAQLNSDSVPRVRVNHLYADILK
QNRNEAIRPLMEQLKEARWLIRNIDQRMVTILRASQAIIDCQQAFLEHGETAVRPLVMREIAEVLGLHESTISRITTQKY
MRTPHGIFELKYFFGSHIPAETGEAHSAIAIRGLIKQLIQNEDRKKPLNDSQVSQILAQQGIVVARRTVAKYREFMHIPP
TNLRKIP

Sequences:

>Translated_487_residues
MKPTLQLKLSTGLTLTPQLKQSIQLLQLSALELNQEISRIVQENPLLELDEDIDQEKLETEGQDSSLVSSDLSTTAEANE
SHDIRENTEISNDWLDDSLYSDQGAFLDTRRQDDEEYDQDYSRLVSKPASLREHLLMQISLRQRCERKKKITALLVDSLD
DNGYFTQDLEELAKLLPVELEIDITDLKSALAYIQQLDPPGVGARNLRECLSIQLSALPDATPLRTEALKLVNEHLESVA
AKNFPLLRKVLGCDETCLQSVYQLITQLNPKPGDDFNATTARYVIPDVIVTKSAKGWVAQLNSDSVPRVRVNHLYADILK
QNRNEAIRPLMEQLKEARWLIRNIDQRMVTILRASQAIIDCQQAFLEHGETAVRPLVMREIAEVLGLHESTISRITTQKY
MRTPHGIFELKYFFGSHIPAETGEAHSAIAIRGLIKQLIQNEDRKKPLNDSQVSQILAQQGIVVARRTVAKYREFMHIPP
TNLRKIP
>Mature_487_residues
MKPTLQLKLSTGLTLTPQLKQSIQLLQLSALELNQEISRIVQENPLLELDEDIDQEKLETEGQDSSLVSSDLSTTAEANE
SHDIRENTEISNDWLDDSLYSDQGAFLDTRRQDDEEYDQDYSRLVSKPASLREHLLMQISLRQRCERKKKITALLVDSLD
DNGYFTQDLEELAKLLPVELEIDITDLKSALAYIQQLDPPGVGARNLRECLSIQLSALPDATPLRTEALKLVNEHLESVA
AKNFPLLRKVLGCDETCLQSVYQLITQLNPKPGDDFNATTARYVIPDVIVTKSAKGWVAQLNSDSVPRVRVNHLYADILK
QNRNEAIRPLMEQLKEARWLIRNIDQRMVTILRASQAIIDCQQAFLEHGETAVRPLVMREIAEVLGLHESTISRITTQKY
MRTPHGIFELKYFFGSHIPAETGEAHSAIAIRGLIKQLIQNEDRKKPLNDSQVSQILAQQGIVVARRTVAKYREFMHIPP
TNLRKIP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=491, Percent_Identity=41.5478615071283, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55253; Mature: 55253

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTLQLKLSTGLTLTPQLKQSIQLLQLSALELNQEISRIVQENPLLELDEDIDQEKLET
CCCCEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHC
EGQDSSLVSSDLSTTAEANESHDIRENTEISNDWLDDSLYSDQGAFLDTRRQDDEEYDQD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCCCCCCCCHHHHHH
YSRLVSKPASLREHLLMQISLRQRCERKKKITALLVDSLDDNGYFTQDLEELAKLLPVEL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEE
EIDITDLKSALAYIQQLDPPGVGARNLRECLSIQLSALPDATPLRTEALKLVNEHLESVA
EEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AKNFPLLRKVLGCDETCLQSVYQLITQLNPKPGDDFNATTARYVIPDVIVTKSAKGWVAQ
HCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHHEECCCCCHHEE
LNSDSVPRVRVNHLYADILKQNRNEAIRPLMEQLKEARWLIRNIDQRMVTILRASQAIID
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQQAFLEHGETAVRPLVMREIAEVLGLHESTISRITTQKYMRTPHGIFELKYFFGSHIPA
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
ETGEAHSAIAIRGLIKQLIQNEDRKKPLNDSQVSQILAQQGIVVARRTVAKYREFMHIPP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
TNLRKIP
CCCCCCC
>Mature Secondary Structure
MKPTLQLKLSTGLTLTPQLKQSIQLLQLSALELNQEISRIVQENPLLELDEDIDQEKLET
CCCCEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHC
EGQDSSLVSSDLSTTAEANESHDIRENTEISNDWLDDSLYSDQGAFLDTRRQDDEEYDQD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCCCCCCCCHHHHHH
YSRLVSKPASLREHLLMQISLRQRCERKKKITALLVDSLDDNGYFTQDLEELAKLLPVEL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEE
EIDITDLKSALAYIQQLDPPGVGARNLRECLSIQLSALPDATPLRTEALKLVNEHLESVA
EEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AKNFPLLRKVLGCDETCLQSVYQLITQLNPKPGDDFNATTARYVIPDVIVTKSAKGWVAQ
HCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHHEECCCCCHHEE
LNSDSVPRVRVNHLYADILKQNRNEAIRPLMEQLKEARWLIRNIDQRMVTILRASQAIID
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQQAFLEHGETAVRPLVMREIAEVLGLHESTISRITTQKYMRTPHGIFELKYFFGSHIPA
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
ETGEAHSAIAIRGLIKQLIQNEDRKKPLNDSQVSQILAQQGIVVARRTVAKYREFMHIPP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
TNLRKIP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]