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The map label for this gene is rlmN

Identifier: 114332136

GI number: 114332136

Start: 2301113

End: 2302252

Strand: Reverse

Name: rlmN

Synonym: Neut_2171

Alternate gene names: 114332136

Gene position: 2302252-2301113 (Counterclockwise)

Preceding gene: 114332137

Following gene: 114332135

Centisome position: 86.52

GC content: 49.47

Gene sequence:

>1140_bases
ATGATTAACCTGCTTGATTTCAATAAAACAGGGCTTATCCATTTTTGCGAAGAAATGGGTGAGAAGCCTTATCGTGCGAG
GCAATTGTTGCGATGGGTACACCGGTTCGGCAAGACGGAATTCATAGAGATGAGTGACCTGGCAAAAACGTTTCGTCAAA
AATTGATGGAGCGTGCTGTTGTTCATCCACCTGAAATTATTAGCGACCATACTGCGGGAGATGGTACTCGTAAATGGTTG
TTATCGACGGGTACGGGGAACGCAGTGGAGATGGTTTTCATTCCCGAGCCTAATCGGGGCACACTTTGTGTTTCCAGTCA
GGTTGGCTGTGCACTGGCATGCAGTTTTTGTTCTACCGGCCGGCAGGGTTTTAATCGTAACTTGAGCGTGGCAGAAATTA
TTGGCCAATTATGGTGGGCTAACCGACTACTCGAAGGCCAGGTTGGTGAGTTATTTTCTCCGGATGTCGCGCAGATTCGG
GCAGATAATACAGACACAAGACGACCAGTTACGAATGTTGTCATGATGGGAATGGGGGAGCCGCTGGCCAATTTCGAAAA
CGTGGTGACCGCACTTGATCTGATGCTGAGTGATGATGCCTATGGTTTATCACGTCGTCGGGTAACGGTGAGTACATCCG
GCCTGGTTCCTGCGTTGGATCGTTTGCGTGAGCGTTGTCCGGTGGCGCTCGCAGTTTCGTTACATGCACCGAATGATGCC
TTGCGTGACCAGTTAGTACCAATCAATAAAAAGTATCCAATCAGGGATTTGCTGGCAGCCTGTGAGCGCTACCTGCCGGC
CGCGCCAAGAGATTTTATTACGTTCGAGTATGTTATGCTGAGAGATGTGAATGACAGCATAGCGCTGGCGCGCGAGCTGG
TGCAGGTAGTCAGAAATATCCCGTGTAAACTCAACCTGATTCCATTCAATACGTTTGCAGGTTCTGGTTATGAGCGATCA
AATACTGATGCTATTGATAATTTCCGGGATGTATTGATGCAAGCAGGAATCGTCACGACTGTGCGTAAAACGCGCGGGGA
TGATATTGCAGCTGCTTGTGGCCAACTGGCAGGACAAGTAAAGGATAAGACGCGCAGAACGGGTACATGTGGATCTGGCC
GAGCCGTGGTTACCGGGTGA

Upstream 100 bases:

>100_bases
CCGGAAACTGCCGCTGTGGAAATAGCCTGTTTCTTCCCATCTTTAGAAGTGTATTCACGCTGAAAACCTGTAGATTTGCC
GATCTAAAAGGCCGGATACA

Downstream 100 bases:

>100_bases
AAAGGTTTATAAATCCAGTGTTGGCGGTGAACAATTGCAAGTAATATGAGCAAGCTGATAAGAGCGGGTGTTGTCGGATT
TCTGGTCTGGCTGGCAGGTT

Product: radical SAM enzyme, Cfr family protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MINLLDFNKTGLIHFCEEMGEKPYRARQLLRWVHRFGKTEFIEMSDLAKTFRQKLMERAVVHPPEIISDHTAGDGTRKWL
LSTGTGNAVEMVFIPEPNRGTLCVSSQVGCALACSFCSTGRQGFNRNLSVAEIIGQLWWANRLLEGQVGELFSPDVAQIR
ADNTDTRRPVTNVVMMGMGEPLANFENVVTALDLMLSDDAYGLSRRRVTVSTSGLVPALDRLRERCPVALAVSLHAPNDA
LRDQLVPINKKYPIRDLLAACERYLPAAPRDFITFEYVMLRDVNDSIALARELVQVVRNIPCKLNLIPFNTFAGSGYERS
NTDAIDNFRDVLMQAGIVTTVRKTRGDDIAAACGQLAGQVKDKTRRTGTCGSGRAVVTG

Sequences:

>Translated_379_residues
MINLLDFNKTGLIHFCEEMGEKPYRARQLLRWVHRFGKTEFIEMSDLAKTFRQKLMERAVVHPPEIISDHTAGDGTRKWL
LSTGTGNAVEMVFIPEPNRGTLCVSSQVGCALACSFCSTGRQGFNRNLSVAEIIGQLWWANRLLEGQVGELFSPDVAQIR
ADNTDTRRPVTNVVMMGMGEPLANFENVVTALDLMLSDDAYGLSRRRVTVSTSGLVPALDRLRERCPVALAVSLHAPNDA
LRDQLVPINKKYPIRDLLAACERYLPAAPRDFITFEYVMLRDVNDSIALARELVQVVRNIPCKLNLIPFNTFAGSGYERS
NTDAIDNFRDVLMQAGIVTTVRKTRGDDIAAACGQLAGQVKDKTRRTGTCGSGRAVVTG
>Mature_379_residues
MINLLDFNKTGLIHFCEEMGEKPYRARQLLRWVHRFGKTEFIEMSDLAKTFRQKLMERAVVHPPEIISDHTAGDGTRKWL
LSTGTGNAVEMVFIPEPNRGTLCVSSQVGCALACSFCSTGRQGFNRNLSVAEIIGQLWWANRLLEGQVGELFSPDVAQIR
ADNTDTRRPVTNVVMMGMGEPLANFENVVTALDLMLSDDAYGLSRRRVTVSTSGLVPALDRLRERCPVALAVSLHAPNDA
LRDQLVPINKKYPIRDLLAACERYLPAAPRDFITFEYVMLRDVNDSIALARELVQVVRNIPCKLNLIPFNTFAGSGYERS
NTDAIDNFRDVLMQAGIVTTVRKTRGDDIAAACGQLAGQVKDKTRRTGTCGSGRAVVTG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=368, Percent_Identity=52.7173913043478, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_NITEC (Q0AE39)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748358.1
- ProteinModelPortal:   Q0AE39
- STRING:   Q0AE39
- GeneID:   4272820
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2171
- NMPDR:   fig|335283.3.peg.222
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q0AE39
- ProtClustDB:   CLSK585184
- BioCyc:   NEUT335283:NEUT_2171-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41963; Mature: 41963

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLLDFNKTGLIHFCEEMGEKPYRARQLLRWVHRFGKTEFIEMSDLAKTFRQKLMERAV
CCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHC
VHPPEIISDHTAGDGTRKWLLSTGTGNAVEMVFIPEPNRGTLCVSSQVGCALACSFCSTG
CCCHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHC
RQGFNRNLSVAEIIGQLWWANRLLEGQVGELFSPDVAQIRADNTDTRRPVTNVVMMGMGE
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHCCCC
PLANFENVVTALDLMLSDDAYGLSRRRVTVSTSGLVPALDRLRERCPVALAVSLHAPNDA
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHH
LRDQLVPINKKYPIRDLLAACERYLPAAPRDFITFEYVMLRDVNDSIALARELVQVVRNI
HHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHCC
PCKLNLIPFNTFAGSGYERSNTDAIDNFRDVLMQAGIVTTVRKTRGDDIAAACGQLAGQV
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHH
KDKTRRTGTCGSGRAVVTG
HHHHHCCCCCCCCCEEECC
>Mature Secondary Structure
MINLLDFNKTGLIHFCEEMGEKPYRARQLLRWVHRFGKTEFIEMSDLAKTFRQKLMERAV
CCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHC
VHPPEIISDHTAGDGTRKWLLSTGTGNAVEMVFIPEPNRGTLCVSSQVGCALACSFCSTG
CCCHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHC
RQGFNRNLSVAEIIGQLWWANRLLEGQVGELFSPDVAQIRADNTDTRRPVTNVVMMGMGE
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHCCCC
PLANFENVVTALDLMLSDDAYGLSRRRVTVSTSGLVPALDRLRERCPVALAVSLHAPNDA
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHH
LRDQLVPINKKYPIRDLLAACERYLPAAPRDFITFEYVMLRDVNDSIALARELVQVVRNI
HHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHCC
PCKLNLIPFNTFAGSGYERSNTDAIDNFRDVLMQAGIVTTVRKTRGDDIAAACGQLAGQV
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHH
KDKTRRTGTCGSGRAVVTG
HHHHHCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA