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The map label for this gene is argG

Identifier: 114332031

GI number: 114332031

Start: 2182828

End: 2184042

Strand: Reverse

Name: argG

Synonym: Neut_2065

Alternate gene names: 114332031

Gene position: 2184042-2182828 (Counterclockwise)

Preceding gene: 114332032

Following gene: 114332030

Centisome position: 82.07

GC content: 49.47

Gene sequence:

>1215_bases
ATGGATAAAGTTAAGAAAGCGGTATTGGCATTTTCGGGGGGGTTGGATACCTCGGTCATACTGAAATGGCTCCAGGATAC
CTATCAGTGCGAGGTGGTTACTTTTACGGCGGATATCGGCCAGGGCGAGGAAATTGAGCCGGCACGCGCCAAGGCCCTGC
AGTTTGGTATCAAGGAAATTTTCATCGAAGATTTGCGTGAAGAATTTGTACGCGACTATGTTTTTCCCATGTTCCGTGCC
AATACGGTTTATGAAGGGGAATATCTGCTTGGTACTAGTATCGCCCGCCCGTTGATTGCCAAACGTCAAGTTGAAATTGC
ACAGCAAACGGGCGCTGATGCGGTCTCTCACGGCGCAACCGGGAAGGGCAACGATCAAGTACGTTTCGAGCTTGGGTATT
ACGCCTTGCAGCCGGATATCCGTGTTATCGCTCCTTGGCGTGAATGGGATTTGACTTCACGTGAAAAACTGCTGGCGTAT
GCCGAGAAACAGGGTATTCCAATTGAAATGAAGAAAAAGCAGGGATCACCGTACAGTATGGATGCCAATCTTTTGCATAT
TTCCTATGAAGGACGTGCACTGGAAGACCCTGCTGTAGAAGCAGAAGAAAGCATGTGGCGCTGGACGATTTCACCGGAAG
CAGCGCCAAACGAACCTGAATATTTGGATCTGGAATACGAGCGGGGAGATATTGTTGCATTGAACGGTGAGAAGCTATCT
CCTGCAGCCGTACTAACAAAACTCAACCAGCTAGGCGGTAAACATGGCATCGGGCGTCTTGATCTGGTGGAAAACCGTTA
TGTTGGAATGAAGTCACGTGGCTGCTATGAAACGCCTGGTGGCACAATTATGCTGCGTGCCCACCGTGCAATCGAATCAA
TCACTCTTGATCGGGAAGTCGCACATTTGAAGGATGATCTGATGCCGCGCTATGCGGCACTGATTTATAACGGTTACTGG
TGGAGCCCAGAAAGAAAATTATTACAGGTGCTTATTGATGAGTCCCAGGCACATGTAAATGGCCAAGTCCGGGTCAAGCT
CTATAAGGGTAACGTGATGGTGGTCGGGCGTGATTCCCGGACAGATTCGCTATTCGATCCTACGATTGCTACTTTTGAGG
AAGACGGAGGGGCTTATCATCAGGCGGATGCAGCGGGCTTCATCAAACTTAATGCGCTGAGAATGCGGATTGCAAAAGCT
CTCCGTAGGCACTGA

Upstream 100 bases:

>100_bases
GGCTACACACACAAAAAGCGTTGATGGAGTACTTGCTGCTGGGCAGGATTAGTGTCAGGTAATCTTTTAATTACCTGTAA
ATTTTTGATTGTAAAGAGAC

Downstream 100 bases:

>100_bases
TTTAGGAAACCGACTATTCCATTATTTCTGTTAACCAAGGTGCTGACTTTAAAACCATACAGTCAGTATCCGTTAGCAGA
GGATAGAGCTACTCAGATCA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MDKVKKAVLAFSGGLDTSVILKWLQDTYQCEVVTFTADIGQGEEIEPARAKALQFGIKEIFIEDLREEFVRDYVFPMFRA
NTVYEGEYLLGTSIARPLIAKRQVEIAQQTGADAVSHGATGKGNDQVRFELGYYALQPDIRVIAPWREWDLTSREKLLAY
AEKQGIPIEMKKKQGSPYSMDANLLHISYEGRALEDPAVEAEESMWRWTISPEAAPNEPEYLDLEYERGDIVALNGEKLS
PAAVLTKLNQLGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIMLRAHRAIESITLDREVAHLKDDLMPRYAALIYNGYW
WSPERKLLQVLIDESQAHVNGQVRVKLYKGNVMVVGRDSRTDSLFDPTIATFEEDGGAYHQADAAGFIKLNALRMRIAKA
LRRH

Sequences:

>Translated_404_residues
MDKVKKAVLAFSGGLDTSVILKWLQDTYQCEVVTFTADIGQGEEIEPARAKALQFGIKEIFIEDLREEFVRDYVFPMFRA
NTVYEGEYLLGTSIARPLIAKRQVEIAQQTGADAVSHGATGKGNDQVRFELGYYALQPDIRVIAPWREWDLTSREKLLAY
AEKQGIPIEMKKKQGSPYSMDANLLHISYEGRALEDPAVEAEESMWRWTISPEAAPNEPEYLDLEYERGDIVALNGEKLS
PAAVLTKLNQLGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIMLRAHRAIESITLDREVAHLKDDLMPRYAALIYNGYW
WSPERKLLQVLIDESQAHVNGQVRVKLYKGNVMVVGRDSRTDSLFDPTIATFEEDGGAYHQADAAGFIKLNALRMRIAKA
LRRH
>Mature_404_residues
MDKVKKAVLAFSGGLDTSVILKWLQDTYQCEVVTFTADIGQGEEIEPARAKALQFGIKEIFIEDLREEFVRDYVFPMFRA
NTVYEGEYLLGTSIARPLIAKRQVEIAQQTGADAVSHGATGKGNDQVRFELGYYALQPDIRVIAPWREWDLTSREKLLAY
AEKQGIPIEMKKKQGSPYSMDANLLHISYEGRALEDPAVEAEESMWRWTISPEAAPNEPEYLDLEYERGDIVALNGEKLS
PAAVLTKLNQLGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIMLRAHRAIESITLDREVAHLKDDLMPRYAALIYNGYW
WSPERKLLQVLIDESQAHVNGQVRVKLYKGNVMVVGRDSRTDSLFDPTIATFEEDGGAYHQADAAGFIKLNALRMRIAKA
LRRH

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=405, Percent_Identity=47.1604938271605, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI16950633, Length=405, Percent_Identity=47.1604938271605, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI1789563, Length=403, Percent_Identity=25.3101736972705, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324514, Length=405, Percent_Identity=45.9259259259259, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21358151, Length=401, Percent_Identity=45.6359102244389, Blast_Score=351, Evalue=6e-97,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_NITEC (Q0AEE4)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748253.1
- ProteinModelPortal:   Q0AEE4
- SMR:   Q0AEE4
- STRING:   Q0AEE4
- GeneID:   4273209
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2065
- NMPDR:   fig|335283.3.peg.2269
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q0AEE4
- ProtClustDB:   PRK00509
- BioCyc:   NEUT335283:NEUT_2065-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45543; Mature: 45543

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 88-88 BINDING 93-93 BINDING 118-118 BINDING 120-120 BINDING 124-124 BINDING 124-124 BINDING 125-125 BINDING 128-128 BINDING 179-179 BINDING 188-188 BINDING 264-264 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVKKAVLAFSGGLDTSVILKWLQDTYQCEVVTFTADIGQGEEIEPARAKALQFGIKEI
CCHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
FIEDLREEFVRDYVFPMFRANTVYEGEYLLGTSIARPLIAKRQVEIAQQTGADAVSHGAT
HHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
GKGNDQVRFELGYYALQPDIRVIAPWREWDLTSREKLLAYAEKQGIPIEMKKKQGSPYSM
CCCCCEEEEEEEEEEECCCCEEEECCHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEE
DANLLHISYEGRALEDPAVEAEESMWRWTISPEAAPNEPEYLDLEYERGDIVALNGEKLS
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCC
PAAVLTKLNQLGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIMLRAHRAIESITLDREV
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCCHHH
AHLKDDLMPRYAALIYNGYWWSPERKLLQVLIDESQAHVNGQVRVKLYKGNVMVVGRDSR
HHHHHHHHHHEEHHEECCEEECHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEECCCC
TDSLFDPTIATFEEDGGAYHQADAAGFIKLNALRMRIAKALRRH
CCCCCCCCCEEEECCCCCEECCCCCCEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKVKKAVLAFSGGLDTSVILKWLQDTYQCEVVTFTADIGQGEEIEPARAKALQFGIKEI
CCHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
FIEDLREEFVRDYVFPMFRANTVYEGEYLLGTSIARPLIAKRQVEIAQQTGADAVSHGAT
HHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
GKGNDQVRFELGYYALQPDIRVIAPWREWDLTSREKLLAYAEKQGIPIEMKKKQGSPYSM
CCCCCEEEEEEEEEEECCCCEEEECCHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEE
DANLLHISYEGRALEDPAVEAEESMWRWTISPEAAPNEPEYLDLEYERGDIVALNGEKLS
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCC
PAAVLTKLNQLGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIMLRAHRAIESITLDREV
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCCHHH
AHLKDDLMPRYAALIYNGYWWSPERKLLQVLIDESQAHVNGQVRVKLYKGNVMVVGRDSR
HHHHHHHHHHEEHHEECCEEECHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEECCCC
TDSLFDPTIATFEEDGGAYHQADAAGFIKLNALRMRIAKALRRH
CCCCCCCCCEEEECCCCCEECCCCCCEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA