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The map label for this gene is pyrE [H]

Identifier: 114332016

GI number: 114332016

Start: 2166695

End: 2167423

Strand: Direct

Name: pyrE [H]

Synonym: Neut_2050

Alternate gene names: 114332016

Gene position: 2166695-2167423 (Clockwise)

Preceding gene: 114332015

Following gene: 114332017

Centisome position: 81.42

GC content: 47.87

Gene sequence:

>729_bases
ATGAAACAGTCTGCTGCATGCAGCTATCAACTTGACTCTGATCATTTAATACACAACCTGATACCTATGGCGGATTTTCG
ACAGGAATTTATTGATTTTGCTCTTCGGTATAACGTCTTGCGTTTTGGCAATTTCAAAACCAAGGCAGGTCGCCCCTCTC
CTTATTTCTTCAATGCCGGCTTGTTCAATGATGGCCAATCGCTGCAACAACTGGGGCAGTTCTACGCTCAGGCCATTCTT
GCTTCTGAGATCCACTTTGATGCCCTGTTTGGCCCTGCCTATAAAGGCATTCCACTAGTCAGTACTGTTGTTATTGCCCT
TGCCAAAGCTGGGTATAACCATCCTTTCAGCTTCAATCGCAAAGAAACCAAGGATCATGGCGAAGGTGGCAATATCGTAG
GTGCTCCGCTGGTCGGGCGTATCTTGATTGTCGATGATGTCATATCGGCAGGCCTGTCTGTCAGTGAATCTGTCACCATG
ATTCGCGCAGCCAGCGCCACACCATGCGGCATCATGGTTGCGCTGGATCGTATGGAAAAAGGCAAAAATGAATGCTCCAC
TTTGCAAGAAATCAGAAACCAATACGACATCCCGGTCATTTCCCTGGTCACACTGGATGATATCGTCGCTTATTTACACA
CCCGGCACGATCTTACCTGCCATATTCCAGCAATAGAAACCTATCGCGCACGCTACGGCGCAAAAATATCGGTTAGCTCC
GGACAGTAG

Upstream 100 bases:

>100_bases
TCAGCGAGTGTTCCTGACCAAGGCAAAAGAATGGAGCAAAATCACCGATCAAAACTATCTGGAGTTACGTTAATTCCGAC
TCTAAAAATACTTTCCCGGA

Downstream 100 bases:

>100_bases
CAGCAACAACGCTGCGGACAGGTACATATCACACGTCATTCCATCACTATTCTTAGCCTGGTTACATCTTTTGTTTAGCT
GTTATTTTCCCCACTTCCGA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKQSAACSYQLDSDHLIHNLIPMADFRQEFIDFALRYNVLRFGNFKTKAGRPSPYFFNAGLFNDGQSLQQLGQFYAQAIL
ASEIHFDALFGPAYKGIPLVSTVVIALAKAGYNHPFSFNRKETKDHGEGGNIVGAPLVGRILIVDDVISAGLSVSESVTM
IRAASATPCGIMVALDRMEKGKNECSTLQEIRNQYDIPVISLVTLDDIVAYLHTRHDLTCHIPAIETYRARYGAKISVSS
GQ

Sequences:

>Translated_242_residues
MKQSAACSYQLDSDHLIHNLIPMADFRQEFIDFALRYNVLRFGNFKTKAGRPSPYFFNAGLFNDGQSLQQLGQFYAQAIL
ASEIHFDALFGPAYKGIPLVSTVVIALAKAGYNHPFSFNRKETKDHGEGGNIVGAPLVGRILIVDDVISAGLSVSESVTM
IRAASATPCGIMVALDRMEKGKNECSTLQEIRNQYDIPVISLVTLDDIVAYLHTRHDLTCHIPAIETYRARYGAKISVSS
GQ
>Mature_242_residues
MKQSAACSYQLDSDHLIHNLIPMADFRQEFIDFALRYNVLRFGNFKTKAGRPSPYFFNAGLFNDGQSLQQLGQFYAQAIL
ASEIHFDALFGPAYKGIPLVSTVVIALAKAGYNHPFSFNRKETKDHGEGGNIVGAPLVGRILIVDDVISAGLSVSESVTM
IRAASATPCGIMVALDRMEKGKNECSTLQEIRNQYDIPVISLVTLDDIVAYLHTRHDLTCHIPAIETYRARYGAKISVSS
GQ

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=146, Percent_Identity=30.8219178082192, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790073, Length=212, Percent_Identity=50.9433962264151, Blast_Score=241, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6323530, Length=224, Percent_Identity=45.9821428571429, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323927, Length=220, Percent_Identity=43.1818181818182, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 26618; Mature: 26618

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSAACSYQLDSDHLIHNLIPMADFRQEFIDFALRYNVLRFGNFKTKAGRPSPYFFNAG
CCCCCCCEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
LFNDGQSLQQLGQFYAQAILASEIHFDALFGPAYKGIPLVSTVVIALAKAGYNHPFSFNR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
KETKDHGEGGNIVGAPLVGRILIVDDVISAGLSVSESVTMIRAASATPCGIMVALDRMEK
CCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCHHHHHHHHHHC
GKNECSTLQEIRNQYDIPVISLVTLDDIVAYLHTRHDLTCHIPAIETYRARYGAKISVSS
CHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCEEEECC
GQ
CC
>Mature Secondary Structure
MKQSAACSYQLDSDHLIHNLIPMADFRQEFIDFALRYNVLRFGNFKTKAGRPSPYFFNAG
CCCCCCCEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
LFNDGQSLQQLGQFYAQAILASEIHFDALFGPAYKGIPLVSTVVIALAKAGYNHPFSFNR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
KETKDHGEGGNIVGAPLVGRILIVDDVISAGLSVSESVTMIRAASATPCGIMVALDRMEK
CCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCHHHHHHHHHHC
GKNECSTLQEIRNQYDIPVISLVTLDDIVAYLHTRHDLTCHIPAIETYRARYGAKISVSS
CHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCEEEECC
GQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]