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The map label for this gene is macA [H]

Identifier: 114331976

GI number: 114331976

Start: 2125309

End: 2126454

Strand: Direct

Name: macA [H]

Synonym: Neut_2010

Alternate gene names: 114331976

Gene position: 2125309-2126454 (Clockwise)

Preceding gene: 114331975

Following gene: 114331977

Centisome position: 79.87

GC content: 49.21

Gene sequence:

>1146_bases
ATGTTCAGTTCACGCGGAAGAATTATCCTTTTCCTGATCATCCTGCTCGCCGCTACACTCTATGGATATTTTTCGCTCCG
GGATGAATCTGGCAATGTACGTTACAAAACACATGTTGTTGATCATGGAGATATTGCGCGCACGATTTCTGCCAACGGCA
CGCTCACCCCACTGGAACTAGTCGAGGTCGGTACACAGATTTCCGGCAGAGTGATCCAGCTGTTTGCAGATTTCAATGAT
GAGGTGAAAACCGGCCAAATCCTGGCAAAACTGGATCCCGCATTGCTTGATGCACAACTACAACAATCTGAGGCTAACCT
AAAAAGCGCACAGACTGCATTATCCGTGGCCATCAATAAAGATAGGCGCAGCCGTAATCTGGTTGAAAGAGGTTTCATCT
CCAGAGAAGCGATGGATGAATCAAAAGAACTGCTCGATTCAGCGCGGGCACAGGTGGTCGTCAGTCAAGCGCAGATTGCC
CGTGACCGTACCAATCTGGATTACAGCATCATCCGCTCTCCCGTTTCCGGTGTTGTCATTGCCCGTAACGTCAATATTGG
ACAAACCGTTGCCGCTAACTTCCAGACACCTATTCTGTTCCAGATTGCGCGTGACCTGAAACAGATGCAAATTGAAATCA
GCGTAGTGGAAGCGGACATTGGCCAGATTCATGATGGCCAGACCATGGTATTCACGGTGGATGCCTTCCAGGATCGTGAA
TTCTCTGCTGTGGTCAAACAAATACGGCTCAATCCCACCATCCAGGAAAATGTGGTGACTTACAGCGTTATTGCGACCGT
TATCAATGAAGATGGCAGCCTGCTGCCTGGCATGACCGCCAATGTACGTTTTATCGTCAATGAAAAATCCGGCGTACTGC
GTGTACCCAATGCGGCTCTGCGTTATAAACCCAGTCGAAAGGAATTTGCCAAACCCACTGACACACAGCCTGGCCAGCGG
TTGCTCTACCGGCTGGAAAACAACCGGCCAGTACCGGTCAAAGTGGCAACAGGCATTACAGATGGCAGCTTCACTGAAAT
TCTTTCAGGTGAGCTCAACGCGAATGACGAACTAATCACCGAGGAAATTGACGACAGCAAACAGGATAAATCGGGCGGCA
GCAACTTCAAGTTCAGAATGTTCTGA

Upstream 100 bases:

>100_bases
GCCGAAGAGAAAAACAAATAAGCACCTTTCAATGATTTGCATCTTCTGTTAAAACCCAGTCATCTGATATTTCTCATACG
ATAAAAAATATTACCCCCCT

Downstream 100 bases:

>100_bases
TGAGCCAGCGATGATGTTTGCAGATCATTCCCACGGCCGGACGGGTGAACCGCTGATAGAGGTGGAAAAACTCTCCAAGA
TTTACTATCTGGGCAATCCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MFSSRGRIILFLIILLAATLYGYFSLRDESGNVRYKTHVVDHGDIARTISANGTLTPLELVEVGTQISGRVIQLFADFND
EVKTGQILAKLDPALLDAQLQQSEANLKSAQTALSVAINKDRRSRNLVERGFISREAMDESKELLDSARAQVVVSQAQIA
RDRTNLDYSIIRSPVSGVVIARNVNIGQTVAANFQTPILFQIARDLKQMQIEISVVEADIGQIHDGQTMVFTVDAFQDRE
FSAVVKQIRLNPTIQENVVTYSVIATVINEDGSLLPGMTANVRFIVNEKSGVLRVPNAALRYKPSRKEFAKPTDTQPGQR
LLYRLENNRPVPVKVATGITDGSFTEILSGELNANDELITEEIDDSKQDKSGGSNFKFRMF

Sequences:

>Translated_381_residues
MFSSRGRIILFLIILLAATLYGYFSLRDESGNVRYKTHVVDHGDIARTISANGTLTPLELVEVGTQISGRVIQLFADFND
EVKTGQILAKLDPALLDAQLQQSEANLKSAQTALSVAINKDRRSRNLVERGFISREAMDESKELLDSARAQVVVSQAQIA
RDRTNLDYSIIRSPVSGVVIARNVNIGQTVAANFQTPILFQIARDLKQMQIEISVVEADIGQIHDGQTMVFTVDAFQDRE
FSAVVKQIRLNPTIQENVVTYSVIATVINEDGSLLPGMTANVRFIVNEKSGVLRVPNAALRYKPSRKEFAKPTDTQPGQR
LLYRLENNRPVPVKVATGITDGSFTEILSGELNANDELITEEIDDSKQDKSGGSNFKFRMF
>Mature_381_residues
MFSSRGRIILFLIILLAATLYGYFSLRDESGNVRYKTHVVDHGDIARTISANGTLTPLELVEVGTQISGRVIQLFADFND
EVKTGQILAKLDPALLDAQLQQSEANLKSAQTALSVAINKDRRSRNLVERGFISREAMDESKELLDSARAQVVVSQAQIA
RDRTNLDYSIIRSPVSGVVIARNVNIGQTVAANFQTPILFQIARDLKQMQIEISVVEADIGQIHDGQTMVFTVDAFQDRE
FSAVVKQIRLNPTIQENVVTYSVIATVINEDGSLLPGMTANVRFIVNEKSGVLRVPNAALRYKPSRKEFAKPTDTQPGQR
LLYRLENNRPVPVKVATGITDGSFTEILSGELNANDELITEEIDDSKQDKSGGSNFKFRMF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082036, Length=325, Percent_Identity=24.3076923076923, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789929, Length=269, Percent_Identity=27.5092936802974, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42189; Mature: 42189

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSRGRIILFLIILLAATLYGYFSLRDESGNVRYKTHVVDHGDIARTISANGTLTPLEL
CCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCEEEEEECCCCCCHHHH
VEVGTQISGRVIQLFADFNDEVKTGQILAKLDPALLDAQLQQSEANLKSAQTALSVAINK
HHHCCCCCCEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
DRRSRNLVERGFISREAMDESKELLDSARAQVVVSQAQIARDRTNLDYSIIRSPVSGVVI
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEE
ARNVNIGQTVAANFQTPILFQIARDLKQMQIEISVVEADIGQIHDGQTMVFTVDAFQDRE
EEECCCCCEEECCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEECCCCCH
FSAVVKQIRLNPTIQENVVTYSVIATVINEDGSLLPGMTANVRFIVNEKSGVLRVPNAAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEECCCHHH
RYKPSRKEFAKPTDTQPGQRLLYRLENNRPVPVKVATGITDGSFTEILSGELNANDELIT
EECCCHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCHHH
EEIDDSKQDKSGGSNFKFRMF
HHHCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MFSSRGRIILFLIILLAATLYGYFSLRDESGNVRYKTHVVDHGDIARTISANGTLTPLEL
CCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCEEEEEECCCCCCHHHH
VEVGTQISGRVIQLFADFNDEVKTGQILAKLDPALLDAQLQQSEANLKSAQTALSVAINK
HHHCCCCCCEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
DRRSRNLVERGFISREAMDESKELLDSARAQVVVSQAQIARDRTNLDYSIIRSPVSGVVI
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEE
ARNVNIGQTVAANFQTPILFQIARDLKQMQIEISVVEADIGQIHDGQTMVFTVDAFQDRE
EEECCCCCEEECCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEECCCCCH
FSAVVKQIRLNPTIQENVVTYSVIATVINEDGSLLPGMTANVRFIVNEKSGVLRVPNAAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEECCCHHH
RYKPSRKEFAKPTDTQPGQRLLYRLENNRPVPVKVATGITDGSFTEILSGELNANDELIT
EECCCHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCHHH
EEIDDSKQDKSGGSNFKFRMF
HHHCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]