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The map label for this gene is ilvE [H]

Identifier: 114331971

GI number: 114331971

Start: 2119318

End: 2120238

Strand: Direct

Name: ilvE [H]

Synonym: Neut_2005

Alternate gene names: 114331971

Gene position: 2119318-2120238 (Clockwise)

Preceding gene: 114331966

Following gene: 114331972

Centisome position: 79.64

GC content: 50.27

Gene sequence:

>921_bases
ATGTCGATGGCGGACCGTGACGGGGTTATCTGGTATGACGGAAAACTGGTTCCGTGGCGCAATGCCACAACACATGTGCT
TACCCATACCCTGCATTATGGAATGGGAGTATTCGAAGGATTGCGCGCCTACCAGACACCCCGGGGAACAGCTATTTTCC
GCTTGCCTGAGCATACAGAACGCCTGTTCAATTCGGCACATGTTTTCAGAATGAAAATCCCTTTTGATCAGGCCACGTTG
ATACAGGCGCAACTGGATGTTGTTCGGGAAAATGGATTGGCCTCTGGCTATATCCGTCCGATTATCTTTTATGGCTCGGA
AGCCATGGGGCTATCTGCCAAGAATCTGTCTGTCCATATTGCAATCGCTGCCTGGTCATGGGGAACCTATCTGGGTGCCG
ATGCACTGGAAAACGGCATTCGCGTGAAGACTTCCTCGTTCACCCGACATCACGTCAATATCAATATGTGTCGCGCCAAA
TCGGTATCCACCTATACCAATTCCATCCTCGCACATCAGGAAGTTGCGCAGGATGGCTACCAGGAAGCCTTGTTGCTGGA
TGTAGATGGTTATGTGGCAGAAGGCTCCGGTGAAAATATTTTCATCATCAAGAAAGACAGGATTTACACGCCTGATCTGA
CATCCTGCCTGGAAGGCATCACACGTGCGACGGTCATTCAGTTGGCGGAAGAAGCCGGCATGGAAGTGGTTCAGAAGCGG
ATTACGCGTGATGAAGTCTATTGCGCAGACGAAGCATTCTTTACCGGCACGGCGGCAGAAATCACACCGATTCGCGAACT
GGATTGCCGCACGATTGGCAATGGCAAACGCGGCCCGATTACCGAGCAGTTGCAAACCTCATTTTTTGATTGTGTCAACG
GCAAATCAGATAAACATGCTGATTGGCTGCATTACGTTTAA

Upstream 100 bases:

>100_bases
CTTAACCGGTCAAAATCCCGATGGATTCAAGAAAAACAATAGCCTGACGTCGGCACTTCCCGTCGTCCAATTTACAGATC
TAATACAGGAGAGTTTCAGC

Downstream 100 bases:

>100_bases
TTGTTAAACCAGACGAAATTTCCTATGAGCACTCCTTCTACAGTCACTTATCCGCAAGCAATTGAAATCCATCCGGATGA
TTTACCACTATACTGCCCAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSMADRDGVIWYDGKLVPWRNATTHVLTHTLHYGMGVFEGLRAYQTPRGTAIFRLPEHTERLFNSAHVFRMKIPFDQATL
IQAQLDVVRENGLASGYIRPIIFYGSEAMGLSAKNLSVHIAIAAWSWGTYLGADALENGIRVKTSSFTRHHVNINMCRAK
SVSTYTNSILAHQEVAQDGYQEALLLDVDGYVAEGSGENIFIIKKDRIYTPDLTSCLEGITRATVIQLAEEAGMEVVQKR
ITRDEVYCADEAFFTGTAAEITPIRELDCRTIGNGKRGPITEQLQTSFFDCVNGKSDKHADWLHYV

Sequences:

>Translated_306_residues
MSMADRDGVIWYDGKLVPWRNATTHVLTHTLHYGMGVFEGLRAYQTPRGTAIFRLPEHTERLFNSAHVFRMKIPFDQATL
IQAQLDVVRENGLASGYIRPIIFYGSEAMGLSAKNLSVHIAIAAWSWGTYLGADALENGIRVKTSSFTRHHVNINMCRAK
SVSTYTNSILAHQEVAQDGYQEALLLDVDGYVAEGSGENIFIIKKDRIYTPDLTSCLEGITRATVIQLAEEAGMEVVQKR
ITRDEVYCADEAFFTGTAAEITPIRELDCRTIGNGKRGPITEQLQTSFFDCVNGKSDKHADWLHYV
>Mature_305_residues
SMADRDGVIWYDGKLVPWRNATTHVLTHTLHYGMGVFEGLRAYQTPRGTAIFRLPEHTERLFNSAHVFRMKIPFDQATLI
QAQLDVVRENGLASGYIRPIIFYGSEAMGLSAKNLSVHIAIAAWSWGTYLGADALENGIRVKTSSFTRHHVNINMCRAKS
VSTYTNSILAHQEVAQDGYQEALLLDVDGYVAEGSGENIFIIKKDRIYTPDLTSCLEGITRATVIQLAEEAGMEVVQKRI
TRDEVYCADEAFFTGTAAEITPIRELDCRTIGNGKRGPITEQLQTSFFDCVNGKSDKHADWLHYV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=43.8127090301003, Blast_Score=272, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17565728, Length=279, Percent_Identity=26.5232974910394, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322002, Length=285, Percent_Identity=25.9649122807018, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322608, Length=330, Percent_Identity=23.3333333333333, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34212; Mature: 34081

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGVIWYDGKLVPWRNATTHVLTHTLHYGMGVFEGLRAYQTPRGTAIFRLPEHTE
CCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHH
RLFNSAHVFRMKIPFDQATLIQAQLDVVRENGLASGYIRPIIFYGSEAMGLSAKNLSVHI
HHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCEEEEE
AIAAWSWGTYLGADALENGIRVKTSSFTRHHVNINMCRAKSVSTYTNSILAHQEVAQDGY
EEEEECCCHHCCHHHHCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCC
QEALLLDVDGYVAEGSGENIFIIKKDRIYTPDLTSCLEGITRATVIQLAEEAGMEVVQKR
CEEEEEEECCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITRDEVYCADEAFFTGTAAEITPIRELDCRTIGNGKRGPITEQLQTSFFDCVNGKSDKHA
HCCCCEEECCCCEECCCCCEECCCHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DWLHYV
CHHCCC
>Mature Secondary Structure 
SMADRDGVIWYDGKLVPWRNATTHVLTHTLHYGMGVFEGLRAYQTPRGTAIFRLPEHTE
CCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHH
RLFNSAHVFRMKIPFDQATLIQAQLDVVRENGLASGYIRPIIFYGSEAMGLSAKNLSVHI
HHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCEEEEE
AIAAWSWGTYLGADALENGIRVKTSSFTRHHVNINMCRAKSVSTYTNSILAHQEVAQDGY
EEEEECCCHHCCHHHHCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCC
QEALLLDVDGYVAEGSGENIFIIKKDRIYTPDLTSCLEGITRATVIQLAEEAGMEVVQKR
CEEEEEEECCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITRDEVYCADEAFFTGTAAEITPIRELDCRTIGNGKRGPITEQLQTSFFDCVNGKSDKHA
HCCCCEEECCCCEECCCCCEECCCHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DWLHYV
CHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]