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The map label for this gene is metG

Identifier: 114331896

GI number: 114331896

Start: 2033051

End: 2035156

Strand: Reverse

Name: metG

Synonym: Neut_1921

Alternate gene names: 114331896

Gene position: 2035156-2033051 (Counterclockwise)

Preceding gene: 114331899

Following gene: 114331878

Centisome position: 76.48

GC content: 49.43

Gene sequence:

>2106_bases
ATGACTGTACGTAATATTCTGGTAACTTCCGCGCTGCCTTATGCTAATGGCAGCATTCATCTGGGGCATCTAGTTGAATA
TATCCAGACCGATATCTGGGTACGTTTCCAAAAAATGCAGGGGCATACCGTCTATTATGTCTGTGCGGATGATGCGCATG
GCACGCCCATCATGCTGCGTGCTGAAAGGGAGGGTATTTCACCGGAAGCATTGATTGCGCGGGTCTATACAGAACATCTG
CGTGATTTTACCGGTTTCCATATCGCGTTTGACCAGTATTACAGCACACATTCCGATGAAACGCGCTACTACGCCGAGGA
TATTTACCAAAAACTGAAAGCAGCCGGGCTGATTGCAATCCGTTCGATAGAACAACTGTATGATCCAGTCAAAAACCTGT
TTCTGCCGGATCGCTTTGTTAAAGGTGAATGCCCGAAATGCGGGGCGGTTGATCAATATGGAGATTCCTGTGAAGCCTGT
GGCGCGGCCTATACACCAACAGAGCTGAAAAATCCCTATTCCGCTATTTCCGGTGCAACGCCCATCCGAAAAGCATCGGA
GCATTTCTTCTTTAAGCTATCTGATCCGCGTTGCAGTGATTTTTTACGTAAATGGACACGCTCAGGCAACCATTTGCAGG
TAGAGGCAGCCAACAAAATGGCAGAATGGCTGGGGGAAGAGGGTGAGAATAAATTATCCGATTGGGATATTTCACGCGAT
GCACCTTATTTTGGCTTTGAAATTCCTGGTGAAACAGACAAGTATTTTTATGTGTGGCTGGATGCACCGATCGGCTACAT
GGGCAGCTTCAAAAAGCTCTGCACCGAGCAAGGGATTGATTTTGATCAATACTGGAAAAAGGGTGCCACCACGGAGTTGT
ATCATTTCATCGGCAAGGATATTCTTTATTTTCATGCACTATTCTGGCCGGCAATGCTGGAGAACGCGGGTTATCGTACC
CCCACACAGATTTTTGCTCATGGTTTTCTGACCGTGAATGGCGAGAAAATGAGTAAATCACGCGGCACGTTTATTACGGC
GGAAAGCTATTTGCAACAGAGGCTGAATCCGGAATGGTTACGCTACTACTATGCCGCCAAGCTCAACGGCAGCATGGAAG
ATATTGATCTTAACCTGGAAGATTTTGTTACGCGTGTCAATGCGGATCTGGTCGGCAAGTACATCAACATTGCCAGCCGT
TGCGCCGGTTTTATCAGCAAATGTTTCGATGGCAAGCTGGTGGCGGGGGAAGATTATCAACTGCTGCAACAAACGGTAGA
TGAATATTTCTCCAGCTGGCAGCCAGGCGTCATCGAAGCAGCCTACGAGGCGCGCGATTTTTCTGCCGCGATACGCCATA
TCATGAAGCGAACGGATGAGGTCAACGAACTGATCCACGTGCTTGCACCCTGGGAAATTGCCAAGGATGAGACACGAGAG
CGCGAATTACACCGCGCCTGCAGCCTGGGGATCCAGATGTTTTATCTACTCTCCTGTTATCTGAAACCGGTTTTGCCCTA
TACCGCTGAACGCATCGAGCATTTTCTCAATTGGATGGCGCTGGGTTGGCCCAGACAGCAAGCAGGACAACCTTTATCCA
CTATGCTGCTGCCGGCAGGTCACACTATTAATACTTATCAACACCTTATCACCCGTATTGATCCCAAACAAATTGCTGCA
CTGACAGAGGCCAACCGACAAACCATGACGACCAGTAACGACACTCATTCACTCACGCGGCATGCTGAAGCACAACAACG
TGTCATTGCTCCCATTGCTGAAACAATTTCAATCGAGGATTTCAGCAAGATCGATCTGCGCATTGCCCGGATTCTTGACG
CACAACATGTTCCCGGTGCCGACAAACTGCTGCAACTGACACTGGATATCGGCTCTGAACAGCGTTCCGTCTTCGCAGGC
ATCAAATCTGCCTATGACCCGGAACAACTCAAAGGACGGCTGACTGTGATGGTCGCTAACCTTGCTCCGCGCAAAATGAA
ATTTGGACTCTCAGAAGGCATGGTGCTGGCTGCAAGCGGTGAAAGCGGCGGCCCATTCTTGCTCGCCCCGGACAGCGGCG
CACAACCAGGCATGCGGGTGAAGTAA

Upstream 100 bases:

>100_bases
AGCGTGCTCCCGCCACAAAAGTATTGATGTTAGCAAATATCGCGCCAGCTGGTTTGCTACAATCTTCTCTTATTATATAT
TTTGAATTGATGAGTAACCG

Downstream 100 bases:

>100_bases
GCGTTTCTGAGAGATACCAACGATTGTACTGTTGTTAATACACTTGTCTGCGGCGTTTGACGATGCTCAGTAACCCCAGG
CCTATCAGCAGTAGAGTGTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MTVRNILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHTVYYVCADDAHGTPIMLRAEREGISPEALIARVYTEHL
RDFTGFHIAFDQYYSTHSDETRYYAEDIYQKLKAAGLIAIRSIEQLYDPVKNLFLPDRFVKGECPKCGAVDQYGDSCEAC
GAAYTPTELKNPYSAISGATPIRKASEHFFFKLSDPRCSDFLRKWTRSGNHLQVEAANKMAEWLGEEGENKLSDWDISRD
APYFGFEIPGETDKYFYVWLDAPIGYMGSFKKLCTEQGIDFDQYWKKGATTELYHFIGKDILYFHALFWPAMLENAGYRT
PTQIFAHGFLTVNGEKMSKSRGTFITAESYLQQRLNPEWLRYYYAAKLNGSMEDIDLNLEDFVTRVNADLVGKYINIASR
CAGFISKCFDGKLVAGEDYQLLQQTVDEYFSSWQPGVIEAAYEARDFSAAIRHIMKRTDEVNELIHVLAPWEIAKDETRE
RELHRACSLGIQMFYLLSCYLKPVLPYTAERIEHFLNWMALGWPRQQAGQPLSTMLLPAGHTINTYQHLITRIDPKQIAA
LTEANRQTMTTSNDTHSLTRHAEAQQRVIAPIAETISIEDFSKIDLRIARILDAQHVPGADKLLQLTLDIGSEQRSVFAG
IKSAYDPEQLKGRLTVMVANLAPRKMKFGLSEGMVLAASGESGGPFLLAPDSGAQPGMRVK

Sequences:

>Translated_701_residues
MTVRNILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHTVYYVCADDAHGTPIMLRAEREGISPEALIARVYTEHL
RDFTGFHIAFDQYYSTHSDETRYYAEDIYQKLKAAGLIAIRSIEQLYDPVKNLFLPDRFVKGECPKCGAVDQYGDSCEAC
GAAYTPTELKNPYSAISGATPIRKASEHFFFKLSDPRCSDFLRKWTRSGNHLQVEAANKMAEWLGEEGENKLSDWDISRD
APYFGFEIPGETDKYFYVWLDAPIGYMGSFKKLCTEQGIDFDQYWKKGATTELYHFIGKDILYFHALFWPAMLENAGYRT
PTQIFAHGFLTVNGEKMSKSRGTFITAESYLQQRLNPEWLRYYYAAKLNGSMEDIDLNLEDFVTRVNADLVGKYINIASR
CAGFISKCFDGKLVAGEDYQLLQQTVDEYFSSWQPGVIEAAYEARDFSAAIRHIMKRTDEVNELIHVLAPWEIAKDETRE
RELHRACSLGIQMFYLLSCYLKPVLPYTAERIEHFLNWMALGWPRQQAGQPLSTMLLPAGHTINTYQHLITRIDPKQIAA
LTEANRQTMTTSNDTHSLTRHAEAQQRVIAPIAETISIEDFSKIDLRIARILDAQHVPGADKLLQLTLDIGSEQRSVFAG
IKSAYDPEQLKGRLTVMVANLAPRKMKFGLSEGMVLAASGESGGPFLLAPDSGAQPGMRVK
>Mature_700_residues
TVRNILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHTVYYVCADDAHGTPIMLRAEREGISPEALIARVYTEHLR
DFTGFHIAFDQYYSTHSDETRYYAEDIYQKLKAAGLIAIRSIEQLYDPVKNLFLPDRFVKGECPKCGAVDQYGDSCEACG
AAYTPTELKNPYSAISGATPIRKASEHFFFKLSDPRCSDFLRKWTRSGNHLQVEAANKMAEWLGEEGENKLSDWDISRDA
PYFGFEIPGETDKYFYVWLDAPIGYMGSFKKLCTEQGIDFDQYWKKGATTELYHFIGKDILYFHALFWPAMLENAGYRTP
TQIFAHGFLTVNGEKMSKSRGTFITAESYLQQRLNPEWLRYYYAAKLNGSMEDIDLNLEDFVTRVNADLVGKYINIASRC
AGFISKCFDGKLVAGEDYQLLQQTVDEYFSSWQPGVIEAAYEARDFSAAIRHIMKRTDEVNELIHVLAPWEIAKDETRER
ELHRACSLGIQMFYLLSCYLKPVLPYTAERIEHFLNWMALGWPRQQAGQPLSTMLLPAGHTINTYQHLITRIDPKQIAAL
TEANRQTMTTSNDTHSLTRHAEAQQRVIAPIAETISIEDFSKIDLRIARILDAQHVPGADKLLQLTLDIGSEQRSVFAGI
KSAYDPEQLKGRLTVMVANLAPRKMKFGLSEGMVLAASGESGGPFLLAPDSGAQPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=575, Percent_Identity=28.8695652173913, Blast_Score=228, Evalue=2e-59,
Organism=Homo sapiens, GI24308436, Length=532, Percent_Identity=25, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI215490011, Length=116, Percent_Identity=37.0689655172414, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI215490009, Length=116, Percent_Identity=37.0689655172414, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI45006986, Length=116, Percent_Identity=37.0689655172414, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788432, Length=706, Percent_Identity=55.6657223796034, Blast_Score=781, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=30.5555555555556, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=569, Percent_Identity=28.4710017574692, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI212645096, Length=531, Percent_Identity=22.2222222222222, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71992781, Length=359, Percent_Identity=23.9554317548747, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321704, Length=570, Percent_Identity=29.1228070175439, Blast_Score=239, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6321610, Length=551, Percent_Identity=22.6860254083485, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24655311, Length=603, Percent_Identity=28.8557213930348, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI21358483, Length=525, Percent_Identity=23.047619047619, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_NITEC (Q0AES9)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748118.1
- ProteinModelPortal:   Q0AES9
- STRING:   Q0AES9
- GeneID:   4273343
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1921
- NMPDR:   fig|335283.3.peg.1045
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- PhylomeDB:   Q0AES9
- ProtClustDB:   PRK00133
- BioCyc:   NEUT335283:NEUT_1921-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 79412; Mature: 79281

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRNILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHTVYYVCADDAHGTPIMLR
CCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCEEEEE
AEREGISPEALIARVYTEHLRDFTGFHIAFDQYYSTHSDETRYYAEDIYQKLKAAGLIAI
ECCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RSIEQLYDPVKNLFLPDRFVKGECPKCGAVDQYGDSCEACGAAYTPTELKNPYSAISGAT
HHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHCCCC
PIRKASEHFFFKLSDPRCSDFLRKWTRSGNHLQVEAANKMAEWLGEEGENKLSDWDISRD
CHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCC
APYFGFEIPGETDKYFYVWLDAPIGYMGSFKKLCTEQGIDFDQYWKKGATTELYHFIGKD
CCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHH
ILYFHALFWPAMLENAGYRTPTQIFAHGFLTVNGEKMSKSRGTFITAESYLQQRLNPEWL
HHHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCHHHHCCCCCEEEHHHHHHHCCCHHHH
RYYYAAKLNGSMEDIDLNLEDFVTRVNADLVGKYINIASRCAGFISKCFDGKLVAGEDYQ
HHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHH
LLQQTVDEYFSSWQPGVIEAAYEARDFSAAIRHIMKRTDEVNELIHVLAPWEIAKDETRE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RELHRACSLGIQMFYLLSCYLKPVLPYTAERIEHFLNWMALGWPRQQAGQPLSTMLLPAG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHEECCC
HTINTYQHLITRIDPKQIAALTEANRQTMTTSNDTHSLTRHAEAQQRVIAPIAETISIED
CCHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FSKIDLRIARILDAQHVPGADKLLQLTLDIGSEQRSVFAGIKSAYDPEQLKGRLTVMVAN
HHHHHHHHHHHHCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHCCCHHHHCCEEEEEEEC
LAPRKMKFGLSEGMVLAASGESGGPFLLAPDSGAQPGMRVK
CCCHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TVRNILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHTVYYVCADDAHGTPIMLR
CHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCEEEEE
AEREGISPEALIARVYTEHLRDFTGFHIAFDQYYSTHSDETRYYAEDIYQKLKAAGLIAI
ECCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RSIEQLYDPVKNLFLPDRFVKGECPKCGAVDQYGDSCEACGAAYTPTELKNPYSAISGAT
HHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHCCCC
PIRKASEHFFFKLSDPRCSDFLRKWTRSGNHLQVEAANKMAEWLGEEGENKLSDWDISRD
CHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCC
APYFGFEIPGETDKYFYVWLDAPIGYMGSFKKLCTEQGIDFDQYWKKGATTELYHFIGKD
CCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHH
ILYFHALFWPAMLENAGYRTPTQIFAHGFLTVNGEKMSKSRGTFITAESYLQQRLNPEWL
HHHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCHHHHCCCCCEEEHHHHHHHCCCHHHH
RYYYAAKLNGSMEDIDLNLEDFVTRVNADLVGKYINIASRCAGFISKCFDGKLVAGEDYQ
HHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHH
LLQQTVDEYFSSWQPGVIEAAYEARDFSAAIRHIMKRTDEVNELIHVLAPWEIAKDETRE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RELHRACSLGIQMFYLLSCYLKPVLPYTAERIEHFLNWMALGWPRQQAGQPLSTMLLPAG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHEECCC
HTINTYQHLITRIDPKQIAALTEANRQTMTTSNDTHSLTRHAEAQQRVIAPIAETISIED
CCHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FSKIDLRIARILDAQHVPGADKLLQLTLDIGSEQRSVFAGIKSAYDPEQLKGRLTVMVAN
HHHHHHHHHHHHCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHCCCHHHHCCEEEEEEEC
LAPRKMKFGLSEGMVLAASGESGGPFLLAPDSGAQPGMRVK
CCCHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA