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The map label for this gene is btuB [H]

Identifier: 114331891

GI number: 114331891

Start: 2027388

End: 2029226

Strand: Direct

Name: btuB [H]

Synonym: Neut_1916

Alternate gene names: 114331891

Gene position: 2027388-2029226 (Clockwise)

Preceding gene: 114331890

Following gene: 114331892

Centisome position: 76.19

GC content: 49.81

Gene sequence:

>1839_bases
ATGTCCAGAAATCATGCTTCATCTGTACTGGCTCTATGGCTGGGAATCAATCTTTCTGCCGTCTCATGGGCGGAAGAAAT
CAACTATCTCGAAAACCCGGTTGTCGTTACCGCTAGCCGTACTGATCAAGCCGCGGAAGATACACTTGCCTCAGTTACCG
TCATTACACGAACTGATATTGAGCGTCAGCAGGCACGTTCAGTGCAAGATCTGCTGCACGGTATCGCAGGTATCAATGTC
GCCAATACCGGTGGCCCTGGAAAATTGACCACACTTTTTCTGCGAGGTACAGAATCCGACCACGTACTGGTTCTGGTTGA
TGGCATCAGAATGGGCTCTGCCACCTCCGGTTTTAGTGAAATCCAGAACTACCCGGTAGAGTTGATTGATCGTATTGAGA
TCGTCCGGGGACCACGTTCCAGCTTATACGGCCCGGAAGCGGTAGGTGGCGTCATCCAGATCTTCACACGTAAAAGCGGA
AAAAAGGGATTAAAACCCACCCTCAGTTTTGGGGGGGGCAGCTATGGAACCATCAATGGCTCAGCTACTCTCTCCGGTAG
CAGCGAACACGCCTGGTTTAATCTCGGCATCAGCGGATCAGACACCAATGGCTTTAATGCCTGCAAAGGTTCTTTTAGTG
CGGGATGCTTCACCGTTGAACCGGACAAGGATGGCTACCGTAATATCTCCGGCTCGGCCCGTGCCGGCTACCACTTCGAT
AGCGGATTGGATGTCGAAGCCAGTTTTTTACACACTGATGGACATTACGAATACGACGGCGGCTTTATCAACCGAACCAA
ACTGGCACAACAGATGTTCGGAGGAACTGCGCGTTATTCGCCTTTTGAGCCTTGGCGCATCACCTTAACAATGGGGCGCA
GTAATGAAGATTCCGATAATTTCAAGGAGAAAATGTTCATGACGCGCTACCACGCCCGGCGCGATACGATCTCCTGGCAA
AATGATATCACTGTAACCCCGAATCAGCTTTTGACCATCGGCACAGATTATCTGTATGATCATGTCAACAGCACGGAAAA
ATTCACCACTACCTCACGCTATAACTGGGGCGTATTTGCACAACACCAGATTTCACTCGCAGCGCACAAACTGCAGCTTT
CTTTACGTCATGATGACAATCAGCAATTCGGCAGCCAGGTAACCGGTGGTGCTTCCTGGGGCTATTCACTGACTGAACGG
GTCAGGCTGACTGCTGGCTTCGGTAGCGCGTTTAAAGCCCCTACCTTCAATGAACTGTATTTCCCTGGATTTGGCAACCC
TCATCTCAAGCCGGAGGATGCCCACACGGTAGAACTGGGTGCAACTGGCCGGTTCGACCGAGGTAATTGGTCACTCAACC
TGTATGAAACCTGGATTGATAACCTGATCTCATATAATGCGGCAACATTCGCTCCAGATAATATAGACAAAGCGCGCATA
CAAGGATTGGAAGCTATCCTCAACACTGAAATCAAAGGCTGGCTGATTCAAACCAACCTCACTTTTCTCAATCCAGAAAA
TCGCTCCAAAGGTGCTAATCGAGGCAATATCCTACCACGACGCAGCAAACAGGCCTTCCGGATTGATGCCAGTCGCCAGC
TTGGTAATTTTGTGGTGGGGGCACTGTTTCTTGCGGAAGCCAATCGTTACGATGATCTGGCAAATAAGCAGCAGCTGGAT
GATTACGTCAAAGTCGATTTACGGGCAGAATATCTGATCAATTCACAGTGGCGCATACAAGGACGAGTTGAAAACCTGTT
TGACAAGGACTATGAAACCGCTGCATTCTTCAACCAACCCGGGCGCAGCTTCTTTGCTACCCTGCGCTATCAACCATAG

Upstream 100 bases:

>100_bases
CCGGATCTTATTCCTGGCATTTAATTATGTCAGGTTCCCTGCTGTTTCTCCGCAATCTGTTTATAACCCGATCATCGCGC
GGGTGTGCGCGGAGGAAACA

Downstream 100 bases:

>100_bases
GATAAAACCATGAAGCCATTACCATTACACTATCAATACCTGATCGGGTTCATCCTGATCGCCCTGATGATTGCCACCCG
CGAATATCACTTCGCCTCAT

Product: TonB-dependent vitamin B12 receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MSRNHASSVLALWLGINLSAVSWAEEINYLENPVVVTASRTDQAAEDTLASVTVITRTDIERQQARSVQDLLHGIAGINV
ANTGGPGKLTTLFLRGTESDHVLVLVDGIRMGSATSGFSEIQNYPVELIDRIEIVRGPRSSLYGPEAVGGVIQIFTRKSG
KKGLKPTLSFGGGSYGTINGSATLSGSSEHAWFNLGISGSDTNGFNACKGSFSAGCFTVEPDKDGYRNISGSARAGYHFD
SGLDVEASFLHTDGHYEYDGGFINRTKLAQQMFGGTARYSPFEPWRITLTMGRSNEDSDNFKEKMFMTRYHARRDTISWQ
NDITVTPNQLLTIGTDYLYDHVNSTEKFTTTSRYNWGVFAQHQISLAAHKLQLSLRHDDNQQFGSQVTGGASWGYSLTER
VRLTAGFGSAFKAPTFNELYFPGFGNPHLKPEDAHTVELGATGRFDRGNWSLNLYETWIDNLISYNAATFAPDNIDKARI
QGLEAILNTEIKGWLIQTNLTFLNPENRSKGANRGNILPRRSKQAFRIDASRQLGNFVVGALFLAEANRYDDLANKQQLD
DYVKVDLRAEYLINSQWRIQGRVENLFDKDYETAAFFNQPGRSFFATLRYQP

Sequences:

>Translated_612_residues
MSRNHASSVLALWLGINLSAVSWAEEINYLENPVVVTASRTDQAAEDTLASVTVITRTDIERQQARSVQDLLHGIAGINV
ANTGGPGKLTTLFLRGTESDHVLVLVDGIRMGSATSGFSEIQNYPVELIDRIEIVRGPRSSLYGPEAVGGVIQIFTRKSG
KKGLKPTLSFGGGSYGTINGSATLSGSSEHAWFNLGISGSDTNGFNACKGSFSAGCFTVEPDKDGYRNISGSARAGYHFD
SGLDVEASFLHTDGHYEYDGGFINRTKLAQQMFGGTARYSPFEPWRITLTMGRSNEDSDNFKEKMFMTRYHARRDTISWQ
NDITVTPNQLLTIGTDYLYDHVNSTEKFTTTSRYNWGVFAQHQISLAAHKLQLSLRHDDNQQFGSQVTGGASWGYSLTER
VRLTAGFGSAFKAPTFNELYFPGFGNPHLKPEDAHTVELGATGRFDRGNWSLNLYETWIDNLISYNAATFAPDNIDKARI
QGLEAILNTEIKGWLIQTNLTFLNPENRSKGANRGNILPRRSKQAFRIDASRQLGNFVVGALFLAEANRYDDLANKQQLD
DYVKVDLRAEYLINSQWRIQGRVENLFDKDYETAAFFNQPGRSFFATLRYQP
>Mature_611_residues
SRNHASSVLALWLGINLSAVSWAEEINYLENPVVVTASRTDQAAEDTLASVTVITRTDIERQQARSVQDLLHGIAGINVA
NTGGPGKLTTLFLRGTESDHVLVLVDGIRMGSATSGFSEIQNYPVELIDRIEIVRGPRSSLYGPEAVGGVIQIFTRKSGK
KGLKPTLSFGGGSYGTINGSATLSGSSEHAWFNLGISGSDTNGFNACKGSFSAGCFTVEPDKDGYRNISGSARAGYHFDS
GLDVEASFLHTDGHYEYDGGFINRTKLAQQMFGGTARYSPFEPWRITLTMGRSNEDSDNFKEKMFMTRYHARRDTISWQN
DITVTPNQLLTIGTDYLYDHVNSTEKFTTTSRYNWGVFAQHQISLAAHKLQLSLRHDDNQQFGSQVTGGASWGYSLTERV
RLTAGFGSAFKAPTFNELYFPGFGNPHLKPEDAHTVELGATGRFDRGNWSLNLYETWIDNLISYNAATFAPDNIDKARIQ
GLEAILNTEIKGWLIQTNLTFLNPENRSKGANRGNILPRRSKQAFRIDASRQLGNFVVGALFLAEANRYDDLANKQQLDD
YVKVDLRAEYLINSQWRIQGRVENLFDKDYETAAFFNQPGRSFFATLRYQP

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=625, Percent_Identity=28.64, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1788478, Length=156, Percent_Identity=39.1025641025641, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1786798, Length=742, Percent_Identity=22.6415094339623, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67942; Mature: 67811

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNHASSVLALWLGINLSAVSWAEEINYLENPVVVTASRTDQAAEDTLASVTVITRTDI
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHEEEEEEECCC
ERQQARSVQDLLHGIAGINVANTGGPGKLTTLFLRGTESDHVLVLVDGIRMGSATSGFSE
HHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCHHH
IQNYPVELIDRIEIVRGPRSSLYGPEAVGGVIQIFTRKSGKKGLKPTLSFGGGSYGTING
HHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEECCCCCCCCCCEEECCCCCEEEECC
SATLSGSSEHAWFNLGISGSDTNGFNACKGSFSAGCFTVEPDKDGYRNISGSARAGYHFD
CEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEECC
SGLDVEASFLHTDGHYEYDGGFINRTKLAQQMFGGTARYSPFEPWRITLTMGRSNEDSDN
CCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHH
FKEKMFMTRYHARRDTISWQNDITVTPNQLLTIGTDYLYDHVNSTEKFTTTSRYNWGVFA
HHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECHHHHHHHCCCCCCEEECCCCCCCEEE
QHQISLAAHKLQLSLRHDDNQQFGSQVTGGASWGYSLTERVRLTAGFGSAFKAPTFNELY
EHHHHEEEEEEEEEEECCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEE
FPGFGNPHLKPEDAHTVELGATGRFDRGNWSLNLYETWIDNLISYNAATFAPDNIDKARI
CCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
QGLEAILNTEIKGWLIQTNLTFLNPENRSKGANRGNILPRRSKQAFRIDASRQLGNFVVG
HHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHH
ALFLAEANRYDDLANKQQLDDYVKVDLRAEYLINSQWRIQGRVENLFDKDYETAAFFNQP
HHHHHCCCCHHHHCCHHHCCHHEEEEEEEEEEECCCEEEEEHHHHHHCCCCCHHHHHCCC
GRSFFATLRYQP
CCEEEEEEEECC
>Mature Secondary Structure 
SRNHASSVLALWLGINLSAVSWAEEINYLENPVVVTASRTDQAAEDTLASVTVITRTDI
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHEEEEEEECCC
ERQQARSVQDLLHGIAGINVANTGGPGKLTTLFLRGTESDHVLVLVDGIRMGSATSGFSE
HHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCHHH
IQNYPVELIDRIEIVRGPRSSLYGPEAVGGVIQIFTRKSGKKGLKPTLSFGGGSYGTING
HHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEECCCCCCCCCCEEECCCCCEEEECC
SATLSGSSEHAWFNLGISGSDTNGFNACKGSFSAGCFTVEPDKDGYRNISGSARAGYHFD
CEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEECC
SGLDVEASFLHTDGHYEYDGGFINRTKLAQQMFGGTARYSPFEPWRITLTMGRSNEDSDN
CCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHH
FKEKMFMTRYHARRDTISWQNDITVTPNQLLTIGTDYLYDHVNSTEKFTTTSRYNWGVFA
HHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECHHHHHHHCCCCCCEEECCCCCCCEEE
QHQISLAAHKLQLSLRHDDNQQFGSQVTGGASWGYSLTERVRLTAGFGSAFKAPTFNELY
EHHHHEEEEEEEEEEECCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEE
FPGFGNPHLKPEDAHTVELGATGRFDRGNWSLNLYETWIDNLISYNAATFAPDNIDKARI
CCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
QGLEAILNTEIKGWLIQTNLTFLNPENRSKGANRGNILPRRSKQAFRIDASRQLGNFVVG
HHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHH
ALFLAEANRYDDLANKQQLDDYVKVDLRAEYLINSQWRIQGRVENLFDKDYETAAFFNQP
HHHHHCCCCHHHHCCHHHCCHHEEEEEEEEEEECCCEEEEEHHHHHHCCCCCHHHHHCCC
GRSFFATLRYQP
CCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA