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The map label for this gene is algU [H]

Identifier: 114331762

GI number: 114331762

Start: 1889936

End: 1890538

Strand: Reverse

Name: algU [H]

Synonym: Neut_1785

Alternate gene names: 114331762

Gene position: 1890538-1889936 (Counterclockwise)

Preceding gene: 114331763

Following gene: 114331761

Centisome position: 71.04

GC content: 45.27

Gene sequence:

>603_bases
ATGAGTGATCGTGAAATCGATCAACAGTTGGTAGAACGGGTACAGCAGGGCGATAAACGTGCTTTTGATCTGCTGGTTAT
TAAATATCAGGGACGGCTCGCGCGCTTGTTATCTCGGTTTATTCGAGATCCGGCTGAAGTGGAAGATATTGCCCAGGAAA
CCTTTATTAAGGCCTATCGGGCATTAGCTTCGTTTCGCGGTGATAGTGCCTTTTATACCTGGTTGTATCGAATTGGAGTT
AATACAGCCAAGAATTTTCTGGTAGCACAAGGCCGTAAACAGCCCGTAACCATTACTGGATTTGATGCTGAAGATGCGGA
AAATTTTGAAGGAGCTGATCAGCTTCGGGAGGTGAATACGCCTGAAAGTGAACTTATGGGAAAACAGGTTGCCCAAACTG
TGAATCAGGCACTAGAAGCTTTACCCGAAGAATTACGAACTGCGATAACTCTCAGGGAAATTGAGGGATTAAGCTACGAA
GAGATTGCCGATATTATGAATTGTCCAATTGGTACAGTGCGGTCCAGAATATTCAGGGCACGTGAGGCGGTGGCTGACAA
ACTGCGTCCTTTGTTAGGCACTGACAAGAGTAAAAGATGGTAA

Upstream 100 bases:

>100_bases
TCAAAAATTTATTGAAAGTCAAAAATAAAACCGGAACAGAAGGTTACAAATTGATAAATAGAGTTGATTTGCATTTTTCT
TATCCCGGCAGCCAAGAAAA

Downstream 100 bases:

>100_bases
TAAGAGGTGAAATGTGAGAAAGAAAGTATCTGAATTAATGGATGGTGAGCTTGAGGATACGGATGCAGTTACGATCATTA
ATGTTGTAAAAAGTGACAGT

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSDREIDQQLVERVQQGDKRAFDLLVIKYQGRLARLLSRFIRDPAEVEDIAQETFIKAYRALASFRGDSAFYTWLYRIGV
NTAKNFLVAQGRKQPVTITGFDAEDAENFEGADQLREVNTPESELMGKQVAQTVNQALEALPEELRTAITLREIEGLSYE
EIADIMNCPIGTVRSRIFRAREAVADKLRPLLGTDKSKRW

Sequences:

>Translated_200_residues
MSDREIDQQLVERVQQGDKRAFDLLVIKYQGRLARLLSRFIRDPAEVEDIAQETFIKAYRALASFRGDSAFYTWLYRIGV
NTAKNFLVAQGRKQPVTITGFDAEDAENFEGADQLREVNTPESELMGKQVAQTVNQALEALPEELRTAITLREIEGLSYE
EIADIMNCPIGTVRSRIFRAREAVADKLRPLLGTDKSKRW
>Mature_199_residues
SDREIDQQLVERVQQGDKRAFDLLVIKYQGRLARLLSRFIRDPAEVEDIAQETFIKAYRALASFRGDSAFYTWLYRIGVN
TAKNFLVAQGRKQPVTITGFDAEDAENFEGADQLREVNTPESELMGKQVAQTVNQALEALPEELRTAITLREIEGLSYEE
IADIMNCPIGTVRSRIFRAREAVADKLRPLLGTDKSKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=192, Percent_Identity=60.4166666666667, Blast_Score=243, Evalue=9e-66,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22799; Mature: 22668

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDREIDQQLVERVQQGDKRAFDLLVIKYQGRLARLLSRFIRDPAEVEDIAQETFIKAYR
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALASFRGDSAFYTWLYRIGVNTAKNFLVAQGRKQPVTITGFDAEDAENFEGADQLREVNT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHCCC
PESELMGKQVAQTVNQALEALPEELRTAITLREIEGLSYEEIADIMNCPIGTVRSRIFRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
REAVADKLRPLLGTDKSKRW
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDREIDQQLVERVQQGDKRAFDLLVIKYQGRLARLLSRFIRDPAEVEDIAQETFIKAYR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALASFRGDSAFYTWLYRIGVNTAKNFLVAQGRKQPVTITGFDAEDAENFEGADQLREVNT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHCCC
PESELMGKQVAQTVNQALEALPEELRTAITLREIEGLSYEEIADIMNCPIGTVRSRIFRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
REAVADKLRPLLGTDKSKRW
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]