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The map label for this gene is radA [H]

Identifier: 114331731

GI number: 114331731

Start: 1854538

End: 1855887

Strand: Reverse

Name: radA [H]

Synonym: Neut_1754

Alternate gene names: 114331731

Gene position: 1855887-1854538 (Counterclockwise)

Preceding gene: 114331733

Following gene: 114331728

Centisome position: 69.74

GC content: 50.81

Gene sequence:

>1350_bases
ATGACGAAACTCAAGACTCTTTATATTTGCAGTGCCTGTGGTGGTCAGGTATTGAAATGGCAGGGCCAGTGTCCGCATTG
TCGTGAATGGAATTCGTTGACTGAAACTGTGCGGGAGAAAAATCTTCCGCATTTCATTCCGGATTCCGCGCAAGGACATG
TGCTGAGTCTGAGTGAGGTCGAGGCGGAGGATATGCCTCGTTTTCTGACCGGGCTGTCCGAGTTTGACCGGGTGTTAGGT
GGAGGCCTGGTACAGGGTGGGGTTGTTTTATTAGGAGGTGATCCCGGCATAGGTAAGTCGACACTACTGTTGCAGGCGCT
ATCCCGGATGTCTGCGCATCACCGAGTGCTATACGTCAGTGGTGAAGAGTCCATGCAACAGGTCGCGCTACGGGCAAAGC
GTCTGACTCTGGATGCCACCAAAGTTGATTTGTTGACCGAAATTCGACTGGAAGCCATTCAGTCAACGTTGGTTGAGCAT
CGCCCTCAAGTCGCAATCATTGATTCGATTCAGACGATTTATTCCGAGTCGTTGCAATCGGCCCCAGGTTCGGTTGCCCA
AGTACGGGAATGTGCCGCTCGCTTGACGCGATTCGCCAAAACCAGTGGTACTTGTGTTGTGCTGGTAGGACATGTGACCA
AAGAGGGGGCGCTGGCTGGGCCGCGTGTTCTGGAGCATATGGTTGATACAGTACTTTATTTTGAAGGAGATACACACTCC
GCTTTTCGTTTAATTCGAGCTTTCAAGAATCGTTTTGGGGCAGTGAACGAACTGGGTGTCTTTGCCATGACCGAGAAGGG
ATTGCGGGAAGTGAATAATCCTTCTGCCTTGTTTCTTTCGCATCATGGTGTGCGTGTGCCGGGTATTTGTGTGATGGTGA
CACAGGAGGGAACGCGCCCTTTGCTGGTGGAGATTCAGGCTCTGGTGGATGAGGCGCATGGATCAACCCCGCGTCGATTG
AGTGTCGGGCTGGAACAGAATCGTCTGGCAATGTTGCTGGCAGTGCTGCATCGCCATGCCGGTATTCCTTGTTTTGATCA
GGATATTTTTATCAATGCGGTGGGTGGAGTCAAAATTACTGAACCAGGCGCTGATCTGGCCGTGATGCTGGCGATTGTTT
CATCTCTGAAAAACCGGATCTTGCCGGAGAAAACTGTTGTGTTTGGAGAAGTTGGTCTAGCGGGGGAAGTACGGCCGGTA
CAACGCGGGCAGGAGCGAATTAAAGAAGCAGCCAAACTGGGATTTACTCAGGCTGTTATTCCCAAGGCAAATCAGTCTAA
ACAAGTTATCAAAGGAATTGAAATTGTTACTGTAGAGCGAATCGAAGAAGCGATTCGTGCGCTTTTCTGA

Upstream 100 bases:

>100_bases
ATATTACATACATATGCTGCGTTCCTGATGAAATATCCGAGTCGGGTGTAAAGCTGGAATATTGTTCTGCATCAGGAAAT
CCCAATCACGTAGTTTATTC

Downstream 100 bases:

>100_bases
TCAGGCAGAGCCCTGGTGTTGTTGGCACAGCTTGATCAGCAAAAGGAGGCTGCTGATACTCTGTCATCGCTCGACTCATA
AACCTCAACGGGCACTACGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTKLKTLYICSACGGQVLKWQGQCPHCREWNSLTETVREKNLPHFIPDSAQGHVLSLSEVEAEDMPRFLTGLSEFDRVLG
GGLVQGGVVLLGGDPGIGKSTLLLQALSRMSAHHRVLYVSGEESMQQVALRAKRLTLDATKVDLLTEIRLEAIQSTLVEH
RPQVAIIDSIQTIYSESLQSAPGSVAQVRECAARLTRFAKTSGTCVVLVGHVTKEGALAGPRVLEHMVDTVLYFEGDTHS
AFRLIRAFKNRFGAVNELGVFAMTEKGLREVNNPSALFLSHHGVRVPGICVMVTQEGTRPLLVEIQALVDEAHGSTPRRL
SVGLEQNRLAMLLAVLHRHAGIPCFDQDIFINAVGGVKITEPGADLAVMLAIVSSLKNRILPEKTVVFGEVGLAGEVRPV
QRGQERIKEAAKLGFTQAVIPKANQSKQVIKGIEIVTVERIEEAIRALF

Sequences:

>Translated_449_residues
MTKLKTLYICSACGGQVLKWQGQCPHCREWNSLTETVREKNLPHFIPDSAQGHVLSLSEVEAEDMPRFLTGLSEFDRVLG
GGLVQGGVVLLGGDPGIGKSTLLLQALSRMSAHHRVLYVSGEESMQQVALRAKRLTLDATKVDLLTEIRLEAIQSTLVEH
RPQVAIIDSIQTIYSESLQSAPGSVAQVRECAARLTRFAKTSGTCVVLVGHVTKEGALAGPRVLEHMVDTVLYFEGDTHS
AFRLIRAFKNRFGAVNELGVFAMTEKGLREVNNPSALFLSHHGVRVPGICVMVTQEGTRPLLVEIQALVDEAHGSTPRRL
SVGLEQNRLAMLLAVLHRHAGIPCFDQDIFINAVGGVKITEPGADLAVMLAIVSSLKNRILPEKTVVFGEVGLAGEVRPV
QRGQERIKEAAKLGFTQAVIPKANQSKQVIKGIEIVTVERIEEAIRALF
>Mature_448_residues
TKLKTLYICSACGGQVLKWQGQCPHCREWNSLTETVREKNLPHFIPDSAQGHVLSLSEVEAEDMPRFLTGLSEFDRVLGG
GLVQGGVVLLGGDPGIGKSTLLLQALSRMSAHHRVLYVSGEESMQQVALRAKRLTLDATKVDLLTEIRLEAIQSTLVEHR
PQVAIIDSIQTIYSESLQSAPGSVAQVRECAARLTRFAKTSGTCVVLVGHVTKEGALAGPRVLEHMVDTVLYFEGDTHSA
FRLIRAFKNRFGAVNELGVFAMTEKGLREVNNPSALFLSHHGVRVPGICVMVTQEGTRPLLVEIQALVDEAHGSTPRRLS
VGLEQNRLAMLLAVLHRHAGIPCFDQDIFINAVGGVKITEPGADLAVMLAIVSSLKNRILPEKTVVFGEVGLAGEVRPVQ
RGQERIKEAAKLGFTQAVIPKANQSKQVIKGIEIVTVERIEEAIRALF

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=449, Percent_Identity=55.456570155902, Blast_Score=511, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48885; Mature: 48754

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLKTLYICSACGGQVLKWQGQCPHCREWNSLTETVREKNLPHFIPDSAQGHVLSLSEV
CCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHC
EAEDMPRFLTGLSEFDRVLGGGLVQGGVVLLGGDPGIGKSTLLLQALSRMSAHHRVLYVS
CHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEE
GEESMQQVALRAKRLTLDATKVDLLTEIRLEAIQSTLVEHRPQVAIIDSIQTIYSESLQS
CHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHC
APGSVAQVRECAARLTRFAKTSGTCVVLVGHVTKEGALAGPRVLEHMVDTVLYFEGDTHS
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEECCCCHH
AFRLIRAFKNRFGAVNELGVFAMTEKGLREVNNPSALFLSHHGVRVPGICVMVTQEGTRP
HHHHHHHHHHHCCCHHHCCHHEECHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCH
LLVEIQALVDEAHGSTPRRLSVGLEQNRLAMLLAVLHRHAGIPCFDQDIFINAVGGVKIT
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEECCEEEE
EPGADLAVMLAIVSSLKNRILPEKTVVFGEVGLAGEVRPVQRGQERIKEAAKLGFTQAVI
CCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHC
PKANQSKQVIKGIEIVTVERIEEAIRALF
CCCCCCHHHHCCCCEEEHHHHHHHHHHHC
>Mature Secondary Structure 
TKLKTLYICSACGGQVLKWQGQCPHCREWNSLTETVREKNLPHFIPDSAQGHVLSLSEV
CCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHC
EAEDMPRFLTGLSEFDRVLGGGLVQGGVVLLGGDPGIGKSTLLLQALSRMSAHHRVLYVS
CHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEE
GEESMQQVALRAKRLTLDATKVDLLTEIRLEAIQSTLVEHRPQVAIIDSIQTIYSESLQS
CHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHC
APGSVAQVRECAARLTRFAKTSGTCVVLVGHVTKEGALAGPRVLEHMVDTVLYFEGDTHS
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEECCCCHH
AFRLIRAFKNRFGAVNELGVFAMTEKGLREVNNPSALFLSHHGVRVPGICVMVTQEGTRP
HHHHHHHHHHHCCCHHHCCHHEECHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCH
LLVEIQALVDEAHGSTPRRLSVGLEQNRLAMLLAVLHRHAGIPCFDQDIFINAVGGVKIT
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEECCEEEE
EPGADLAVMLAIVSSLKNRILPEKTVVFGEVGLAGEVRPVQRGQERIKEAAKLGFTQAVI
CCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHC
PKANQSKQVIKGIEIVTVERIEEAIRALF
CCCCCCHHHHCCCCEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]