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The map label for this gene is flhA [H]

Identifier: 114331376

GI number: 114331376

Start: 1455948

End: 1457054

Strand: Direct

Name: flhA [H]

Synonym: Neut_1385

Alternate gene names: 114331376

Gene position: 1455948-1457054 (Clockwise)

Preceding gene: 114331374

Following gene: 114331377

Centisome position: 54.71

GC content: 51.58

Gene sequence:

>1107_bases
ATGAAAACACATGCAGCAGTTGCCTGGCAGGCTGGCCAGCCCCTTACCATTGAAGAGGTTGATCTTGCCGCTCCGCGCGC
GGGAGAGGTGCTGATTGAAGTTAAAGCCACGGGTATCTGCCATACTGATTACTACACACTTTCCGGCGCTGATCCGGAAG
GCCTGTTCCCTGCAATTCTTGGCCACGAAGGTGCCGGAGTTGTGGTTGAAACGGGTACAGATGTCAAATCACTGCGCCCG
GGTGATCATGTGATTCCCTTGTACACACCGGAATGTCGGGAATGCAAATCTTGTCTTTCGCGTAAAACCAACTTATGTCA
GGCGATCCGTGCGACACAAGGGCGCGGCCTGATGCCGGACGGCACTTCGCGCTTCTCGCTCAACGGCAAACCCATTATGC
ACTATATGGGCACTTCAACTTTTTCTAATTACATCGTTGTCCCTGAGATTGCTCTGGCTAAGATTCGTGAAGATGCCCCT
TTTGACAAAGCCTGCTACATCGGCTGCGGGGTGACCACCGGTATTGGTGCGGTATTGTTCACCGCCAAAGTAGAAACGGG
TGCAAACGTCGCTGTATTCGGGCTAGGCGGCATCGGCCTGAATGTTATTCAGGGTGCGCGCATGGTCGGTGCGGACAAGA
TTATCGGAGTTGATATCAATCCGGAGCGGGAAGCATTAGGCCGACAATTCGGCATGACCCATTTCATCAACCCGAAGGAA
GTAGAAAACGTGGTGGATGCAGTAATACAGCTGACGGATGGAGGTGCAGATTACAGCTTTGAATGTATCGGCAATACCAC
TGTTATGCGCCAAGCGCTGGAATGCACACACAGAGGCTGGGGGCGCAGTATCATTATTGGTGTAGCAGAAGCTGGCGCCG
AGATCAGTACCCGCCCATTTCAGCTGGTGACTGGTAGAAAATGGGAGGGTACTGCTTTTGGTGGCGCACGTGGCCGCTCG
GATGTACCACGTATTGTTGACTGGTATATGGAGGGAAAAATCAGTATTGATCCGCTGATTACACACACCCTGAAACTGGA
AGACATCAACGAAGGTTTCAAATTGATGGAAGCCGGAAAATCTATTCGTTCTGTTGTAATTTACTGA

Upstream 100 bases:

>100_bases
CTTTCATCCGGCCTTTCGCAAAGAACTAGATTGCACGGCCGTGTCAGTCACATACAATGACTGCTTTCATCAAACTTCTC
CTAAAAGGATGATCTCCGTC

Downstream 100 bases:

>100_bases
TGACTTAAAAAAATGACAATCAAACTGGAAATTCGCAGCCAGCACCGCTGTTTTGGTGGTCTTCAAAGTTATTATCAGCA
TCATTCAGAAATTATCGGCT

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTHAAVAWQAGQPLTIEEVDLAAPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVVVETGTDVKSLRP
GDHVIPLYTPECRECKSCLSRKTNLCQAIRATQGRGLMPDGTSRFSLNGKPIMHYMGTSTFSNYIVVPEIALAKIREDAP
FDKACYIGCGVTTGIGAVLFTAKVETGANVAVFGLGGIGLNVIQGARMVGADKIIGVDINPEREALGRQFGMTHFINPKE
VENVVDAVIQLTDGGADYSFECIGNTTVMRQALECTHRGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGTAFGGARGRS
DVPRIVDWYMEGKISIDPLITHTLKLEDINEGFKLMEAGKSIRSVVIY

Sequences:

>Translated_368_residues
MKTHAAVAWQAGQPLTIEEVDLAAPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVVVETGTDVKSLRP
GDHVIPLYTPECRECKSCLSRKTNLCQAIRATQGRGLMPDGTSRFSLNGKPIMHYMGTSTFSNYIVVPEIALAKIREDAP
FDKACYIGCGVTTGIGAVLFTAKVETGANVAVFGLGGIGLNVIQGARMVGADKIIGVDINPEREALGRQFGMTHFINPKE
VENVVDAVIQLTDGGADYSFECIGNTTVMRQALECTHRGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGTAFGGARGRS
DVPRIVDWYMEGKISIDPLITHTLKLEDINEGFKLMEAGKSIRSVVIY
>Mature_368_residues
MKTHAAVAWQAGQPLTIEEVDLAAPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVVVETGTDVKSLRP
GDHVIPLYTPECRECKSCLSRKTNLCQAIRATQGRGLMPDGTSRFSLNGKPIMHYMGTSTFSNYIVVPEIALAKIREDAP
FDKACYIGCGVTTGIGAVLFTAKVETGANVAVFGLGGIGLNVIQGARMVGADKIIGVDINPEREALGRQFGMTHFINPKE
VENVVDAVIQLTDGGADYSFECIGNTTVMRQALECTHRGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGTAFGGARGRS
DVPRIVDWYMEGKISIDPLITHTLKLEDINEGFKLMEAGKSIRSVVIY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=59.9455040871935, Blast_Score=450, Evalue=1e-127,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=45.9893048128342, Blast_Score=351, Evalue=5e-97,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=45.7994579945799, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.5284552845528, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=44.9864498644986, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=43.4782608695652, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=43.5135135135135, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=43.5135135135135, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=43.7673130193906, Blast_Score=326, Evalue=2e-89,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=60.7046070460705, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI87082125, Length=381, Percent_Identity=26.509186351706, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788073, Length=364, Percent_Identity=26.3736263736264, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790045, Length=362, Percent_Identity=27.3480662983425, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786825, Length=383, Percent_Identity=26.6318537859008, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=25.9365994236311, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI226510992, Length=358, Percent_Identity=23.7430167597765, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788075, Length=369, Percent_Identity=26.2872628726287, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786518, Length=374, Percent_Identity=24.331550802139, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=53.887399463807, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=53.6, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=54.5994065281899, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17562584, Length=369, Percent_Identity=27.6422764227642, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17562876, Length=355, Percent_Identity=26.7605633802817, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=27.4932614555256, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562582, Length=359, Percent_Identity=26.1838440111421, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.7169811320755, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319621, Length=373, Percent_Identity=28.9544235924933, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324486, Length=369, Percent_Identity=27.3712737127371, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=28.686327077748, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=361, Percent_Identity=27.7008310249307, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=27.1978021978022, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=361, Percent_Identity=27.1468144044321, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=361, Percent_Identity=27.1468144044321, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17737895, Length=367, Percent_Identity=58.0381471389646, Blast_Score=455, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39501; Mature: 39501

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTHAAVAWQAGQPLTIEEVDLAAPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAIL
CCCCEEEEECCCCCCEEEECCCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVVVETGTDVKSLRPGDHVIPLYTPECRECKSCLSRKTNLCQAIRATQGRGLMPD
CCCCCEEEEECCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GTSRFSLNGKPIMHYMGTSTFSNYIVVPEIALAKIREDAPFDKACYIGCGVTTGIGAVLF
CCCEEEECCCEEEEEECCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCHHHHHCEEEE
TAKVETGANVAVFGLGGIGLNVIQGARMVGADKIIGVDINPEREALGRQFGMTHFINPKE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCHHH
VENVVDAVIQLTDGGADYSFECIGNTTVMRQALECTHRGWGRSIIIGVAEAGAEISTRPF
HHHHHHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGRKWEGTAFGGARGRSDVPRIVDWYMEGKISIDPLITHTLKLEDINEGFKLMEAGK
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEEEEHHCCHHHHHHHCH
SIRSVVIY
HHHEEECC
>Mature Secondary Structure
MKTHAAVAWQAGQPLTIEEVDLAAPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAIL
CCCCEEEEECCCCCCEEEECCCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVVVETGTDVKSLRPGDHVIPLYTPECRECKSCLSRKTNLCQAIRATQGRGLMPD
CCCCCEEEEECCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GTSRFSLNGKPIMHYMGTSTFSNYIVVPEIALAKIREDAPFDKACYIGCGVTTGIGAVLF
CCCEEEECCCEEEEEECCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCHHHHHCEEEE
TAKVETGANVAVFGLGGIGLNVIQGARMVGADKIIGVDINPEREALGRQFGMTHFINPKE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCHHH
VENVVDAVIQLTDGGADYSFECIGNTTVMRQALECTHRGWGRSIIIGVAEAGAEISTRPF
HHHHHHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGRKWEGTAFGGARGRSDVPRIVDWYMEGKISIDPLITHTLKLEDINEGFKLMEAGK
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEEEEHHCCHHHHHHHCH
SIRSVVIY
HHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]