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The map label for this gene is potF [H]

Identifier: 114331324

GI number: 114331324

Start: 1393757

End: 1394866

Strand: Direct

Name: potF [H]

Synonym: Neut_1331

Alternate gene names: 114331324

Gene position: 1393757-1394866 (Clockwise)

Preceding gene: 114331323

Following gene: 114331325

Centisome position: 52.38

GC content: 50.81

Gene sequence:

>1110_bases
GTGAAACTCGGCTACACCCTGATGGGTCTCTTTTGCCTGCTGATCATCGCGCTGACCGGCTGCACATCCGAAGATCAGGT
TGCGGGTAATTTGTCCGGCAAAAATGGCAATATTCTGAGACTGTTCAACTGGAATAACTACATCAGCCAGCAAACAATCG
AGCGTTTTGAGCAGACCTGTCAATGCCGGGTGACACAGGACTATTACTCAGATAACGAGGAATTGCTTGCCAAACTGGCA
GCTGGTGCAACCGGCTATGACTTGCTCGTTCCAACCGGTGATGCCATGGATACCCTGATTCATCAGGGGGCACTAAACGT
GCTGGACAAATCACAAATCCCCAACCTGAAGAATATTGACCCAAGCTATCTGGGCACTGTGTTCGATCCGGATAACCGCT
ACTCCGTCCCCTATGCCTACACCACGACATTAATAGGATTTAACCGCGAGAAACTGGCCGAACTAAACCTGCCTACCGAT
AGCTGGGCGATCATCTTCGAGCCTGAGTATCTGAAAAAAATCAAGGGACGTATCACCGTACTCGACAGCCAGCGCGAACT
GATGGCAGCCGCACTCAAATATCTGGGCTATTCCGCTAGTGATACTGATGAAAATCACTGGCAGCAGGCAAAGGCGCTGA
TTATCCGGGCAAAACCTTACTGGGCCGCTTTCAGCAACACTAGCTATATCCGCGAACTGGCAATTGGTAACCTTTGGGTG
GCTCACGGCTATTCAAACGATATGTATCAGGCTGCACTGGATGCAAAAAAAACCGGACGCCGCTTTAGCGTGGATTTTGT
CATTCCCCGTGAAGGCGCAGTGCTGTCACTCGACAGCATGGTGCTCCATCACAGTGGCCATCGTCCTGATCTGGCCCACC
GCTTCATCAATTTCATGCTGGAAGGTAAAAACTCCGCTGAACTGACCAACCTGACCGGTTCCGGCAACCCCAATCTGGCT
GCTCGGCCCTATATCCATCCTGAAATTATCGCCAATCATGCTATTTTCCCGGATACAGCACAACTGTCCCGACTGGAAAT
GCTGCATGATATCAATCACACACAACGCCGGCTGCTCAGCCGAATCTGGACAGAAATCAAACTGAGGTGA

Upstream 100 bases:

>100_bases
AAGTTAACGCCATTTCTACATTATTCATGTTGTTGACACTGGTATTGATCATCATCGCTAATCGTCTGGATTCCGGCATC
TTGCAGGGGGAAAAGAAATC

Downstream 100 bases:

>100_bases
AAGCATGAGCTATTATCAACATCACGTTTTCTTTTGCGTTAACCAACGTGGCAACTGTGAAGGCTGCTGTAATGATTATC
ACGCCCAGGCAATGCGCGAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKLGYTLMGLFCLLIIALTGCTSEDQVAGNLSGKNGNILRLFNWNNYISQQTIERFEQTCQCRVTQDYYSDNEELLAKLA
AGATGYDLLVPTGDAMDTLIHQGALNVLDKSQIPNLKNIDPSYLGTVFDPDNRYSVPYAYTTTLIGFNREKLAELNLPTD
SWAIIFEPEYLKKIKGRITVLDSQRELMAAALKYLGYSASDTDENHWQQAKALIIRAKPYWAAFSNTSYIRELAIGNLWV
AHGYSNDMYQAALDAKKTGRRFSVDFVIPREGAVLSLDSMVLHHSGHRPDLAHRFINFMLEGKNSAELTNLTGSGNPNLA
ARPYIHPEIIANHAIFPDTAQLSRLEMLHDINHTQRRLLSRIWTEIKLR

Sequences:

>Translated_369_residues
MKLGYTLMGLFCLLIIALTGCTSEDQVAGNLSGKNGNILRLFNWNNYISQQTIERFEQTCQCRVTQDYYSDNEELLAKLA
AGATGYDLLVPTGDAMDTLIHQGALNVLDKSQIPNLKNIDPSYLGTVFDPDNRYSVPYAYTTTLIGFNREKLAELNLPTD
SWAIIFEPEYLKKIKGRITVLDSQRELMAAALKYLGYSASDTDENHWQQAKALIIRAKPYWAAFSNTSYIRELAIGNLWV
AHGYSNDMYQAALDAKKTGRRFSVDFVIPREGAVLSLDSMVLHHSGHRPDLAHRFINFMLEGKNSAELTNLTGSGNPNLA
ARPYIHPEIIANHAIFPDTAQLSRLEMLHDINHTQRRLLSRIWTEIKLR
>Mature_369_residues
MKLGYTLMGLFCLLIIALTGCTSEDQVAGNLSGKNGNILRLFNWNNYISQQTIERFEQTCQCRVTQDYYSDNEELLAKLA
AGATGYDLLVPTGDAMDTLIHQGALNVLDKSQIPNLKNIDPSYLGTVFDPDNRYSVPYAYTTTLIGFNREKLAELNLPTD
SWAIIFEPEYLKKIKGRITVLDSQRELMAAALKYLGYSASDTDENHWQQAKALIIRAKPYWAAFSNTSYIRELAIGNLWV
AHGYSNDMYQAALDAKKTGRRFSVDFVIPREGAVLSLDSMVLHHSGHRPDLAHRFINFMLEGKNSAELTNLTGSGNPNLA
ARPYIHPEIIANHAIFPDTAQLSRLEMLHDINHTQRRLLSRIWTEIKLR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=336, Percent_Identity=33.9285714285714, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=30.3405572755418, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787711, Length=311, Percent_Identity=26.6881028938907, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41633; Mature: 41633

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGYTLMGLFCLLIIALTGCTSEDQVAGNLSGKNGNILRLFNWNNYISQQTIERFEQTC
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
QCRVTQDYYSDNEELLAKLAAGATGYDLLVPTGDAMDTLIHQGALNVLDKSQIPNLKNID
EEEEEHHHHCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PSYLGTVFDPDNRYSVPYAYTTTLIGFNREKLAELNLPTDSWAIIFEPEYLKKIKGRITV
HHHCCEEECCCCCEECCEEEEEEEEECCHHHHHCCCCCCCCEEEEECHHHHHHHCCEEEE
LDSQRELMAAALKYLGYSASDTDENHWQQAKALIIRAKPYWAAFSNTSYIRELAIGNLWV
ECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCEEECCCCHHHHHHHHCCEEE
AHGYSNDMYQAALDAKKTGRRFSVDFVIPREGAVLSLDSMVLHHSGHRPDLAHRFINFML
EECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHH
EGKNSAELTNLTGSGNPNLAARPYIHPEIIANHAIFPDTAQLSRLEMLHDINHTQRRLLS
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
RIWTEIKLR
HHHHHHCCC
>Mature Secondary Structure
MKLGYTLMGLFCLLIIALTGCTSEDQVAGNLSGKNGNILRLFNWNNYISQQTIERFEQTC
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
QCRVTQDYYSDNEELLAKLAAGATGYDLLVPTGDAMDTLIHQGALNVLDKSQIPNLKNID
EEEEEHHHHCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PSYLGTVFDPDNRYSVPYAYTTTLIGFNREKLAELNLPTDSWAIIFEPEYLKKIKGRITV
HHHCCEEECCCCCEECCEEEEEEEEECCHHHHHCCCCCCCCEEEEECHHHHHHHCCEEEE
LDSQRELMAAALKYLGYSASDTDENHWQQAKALIIRAKPYWAAFSNTSYIRELAIGNLWV
ECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCEEECCCCHHHHHHHHCCEEE
AHGYSNDMYQAALDAKKTGRRFSVDFVIPREGAVLSLDSMVLHHSGHRPDLAHRFINFML
EECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHH
EGKNSAELTNLTGSGNPNLAARPYIHPEIIANHAIFPDTAQLSRLEMLHDINHTQRRLLS
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
RIWTEIKLR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]