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The map label for this gene is amyA [H]

Identifier: 114331288

GI number: 114331288

Start: 1357884

End: 1359908

Strand: Direct

Name: amyA [H]

Synonym: Neut_1294

Alternate gene names: 114331288

Gene position: 1357884-1359908 (Clockwise)

Preceding gene: 114331287

Following gene: 114331289

Centisome position: 51.03

GC content: 48.99

Gene sequence:

>2025_bases
ATGACCTCATCCGTATCATTGCTGTTTGGTGTCCACGCCCACCAACCGATTGGCAATTTCCCCGAGGTCGTTATTGATGC
GCATGAACGCTGTTACAAGCCCTTCCTGCAAACTTTATACCGCTATCCGGAATTTCGTTTTGCCATCCACTTTTCCGGCT
GGTTGCTGGATTTTCTATTTGAGCACTACCCGAAAGATATGGCGTTATTACGTGAGATGGTCACACGCGGGCAGATCGAA
ATATTTGGTGCAGGCGATACCGAGCCGGTACTGGCGGCCATTCCCGAGCGGGATCGCATCGGCCAGCTCAAGACCTTTTC
GGCTAAACTCAAGAAAAAGCTGGGGCAGTCGCCACAAGGCGCCTGGCTAACTGAGCGTGTATGGGAATCCACTATCGTGC
CTGCTTTGGTTAATTGCGGTATCCATTATGTAATAGTGGACGATTACCACTTCATTTGTACCGGAAAAAATAAAATAGAA
CTTGATGGTTACTTCAGTACTGAAGAAGATCATCACAAGCTTGATCTTTTTCCCATCTCCGAAGCATTGCGTTACCGGCT
CCCCTTTTCTCCTGCGCTGGAAGCCATTGCCTATATTGAATCCCTGATTGATCAGTCCACAACCGAGCATCAACCTGCAG
CAGTCTATTTTGACGACATTGAGAAATTCGGCATCTGGCCAGAAACTTATCAGTGGGTTTACGAACACGGCTGGCTGGAG
AAATTTATCCAGGGTGTACTGGCTTCTCCTCATATCCAGCTGCAGCACTACCGGGATTATCATGCCAGCGAAAAAACCCG
TGGCATCGTTTACCTCCCCACCACCTCCTACATCGAGATGAATGAATGGACGCTGCCCGCTGAACCTGCTCATATTTATG
ATGATCTGGTGCAGCAAGCCAAATCCACAGGTCAGTACGAACAAACCAGATCTTTCCTGCGCGGAGGAATGTGGAAAAAC
TTTTTTTCACGCTACCCTGAATCCAACTGGATGCACAAGCGCATGCTCGGCCTGTCCGCACGTTACGCCAGCCTGCCGGC
CAGGCAGCGGACCTCTGCCATGCAACGATGCCTGTACGCTTCCCAGGCCAATGATGCCTACTGGCATGGTCTATTTGGCG
GTCTGTATCTGCCGCATTTGCGGCGCACCGTCTACAACAATCTGATTGAACTGGAAGCGCAACTCGATCAGTGTGAATCG
CGCAATATCTACTATCATGAGGATACTGATCTGGATGGCACGGAGGAAATCTATCTGCAAAACGGGACTGTGCAGGCAGT
TTTGAAACTCAACAGCAGCGCCAGCATCTGCGAACTGGACAGCTATCCACTGAAACACAACTTTGCCGATACCCTCGCCC
GCCAGACTGAGCATTACTACCGAAAGATCCAACCGGGTCTACAGGGAGCTTCATCTCATCAGCAATCAGGAATAGCTTCA
GCACATGATCGAGTCAATTTCAAACATGCTATTAGCCAGGCAGATATGGAACCGGATCTGTATGCACAACACCTGTTTAT
TGATCGTTTAAACGGTGAGCTGCTGGATTATCAACTGCACCCCTCAGATAAGGCAGATGAAGTTATTTTTCAGGCGATAA
CAGATTTCGGGTCAATCAGTAAATGCTTTCGGATTATCAGCAACCAGCTACTTGTCATCTATGTATGCAGCGGGGAAGCC
AGTGGATACATGCAGACAGAAATCAATCTGGCCATGCCCAGTTGCGACGGACCGGGCGGACGTTACATCCGGAATCACAC
TGTGCTGTCCGGCTTCGGACAACCCATCGAACTGGATAATCTGACAGCCATTACACTGGAAGACGACACGCTGGGCGGCA
GTCTGTCACTTGATATTTCACCTGTAACAAAATTTTATGCCTGCCCGCACTTCACCGTTTCTCAATCGGAAGGCGGATTT
GAAAAAATCATGCAGGCAGTCAAACTAATACTTACCTGGAAGATAACGGATGACCAGCAGCAACATATTACCCTTCGTTT
TGATGAAATGAACACTACTGTTTGA

Upstream 100 bases:

>100_bases
CAGGCAAAGGTGGCGCGATGCGGCATGCCTCTTAGAGGGCTCACAAAAAATGTAATACTGGCAATATGAAACAACCGATA
AATATTTAAGGATATCCCAG

Downstream 100 bases:

>100_bases
TCCGGCCCAGATAAAGAATAATGGAGCTACCATGATAGAAGAATCCGGTCGTCTCAAACTATGTGATGAAGAGCAGATTC
CCTGGAAAAAATGGGGTCCG

Product: 4-alpha-glucanotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MTSSVSLLFGVHAHQPIGNFPEVVIDAHERCYKPFLQTLYRYPEFRFAIHFSGWLLDFLFEHYPKDMALLREMVTRGQIE
IFGAGDTEPVLAAIPERDRIGQLKTFSAKLKKKLGQSPQGAWLTERVWESTIVPALVNCGIHYVIVDDYHFICTGKNKIE
LDGYFSTEEDHHKLDLFPISEALRYRLPFSPALEAIAYIESLIDQSTTEHQPAAVYFDDIEKFGIWPETYQWVYEHGWLE
KFIQGVLASPHIQLQHYRDYHASEKTRGIVYLPTTSYIEMNEWTLPAEPAHIYDDLVQQAKSTGQYEQTRSFLRGGMWKN
FFSRYPESNWMHKRMLGLSARYASLPARQRTSAMQRCLYASQANDAYWHGLFGGLYLPHLRRTVYNNLIELEAQLDQCES
RNIYYHEDTDLDGTEEIYLQNGTVQAVLKLNSSASICELDSYPLKHNFADTLARQTEHYYRKIQPGLQGASSHQQSGIAS
AHDRVNFKHAISQADMEPDLYAQHLFIDRLNGELLDYQLHPSDKADEVIFQAITDFGSISKCFRIISNQLLVIYVCSGEA
SGYMQTEINLAMPSCDGPGGRYIRNHTVLSGFGQPIELDNLTAITLEDDTLGGSLSLDISPVTKFYACPHFTVSQSEGGF
EKIMQAVKLILTWKITDDQQQHITLRFDEMNTTV

Sequences:

>Translated_674_residues
MTSSVSLLFGVHAHQPIGNFPEVVIDAHERCYKPFLQTLYRYPEFRFAIHFSGWLLDFLFEHYPKDMALLREMVTRGQIE
IFGAGDTEPVLAAIPERDRIGQLKTFSAKLKKKLGQSPQGAWLTERVWESTIVPALVNCGIHYVIVDDYHFICTGKNKIE
LDGYFSTEEDHHKLDLFPISEALRYRLPFSPALEAIAYIESLIDQSTTEHQPAAVYFDDIEKFGIWPETYQWVYEHGWLE
KFIQGVLASPHIQLQHYRDYHASEKTRGIVYLPTTSYIEMNEWTLPAEPAHIYDDLVQQAKSTGQYEQTRSFLRGGMWKN
FFSRYPESNWMHKRMLGLSARYASLPARQRTSAMQRCLYASQANDAYWHGLFGGLYLPHLRRTVYNNLIELEAQLDQCES
RNIYYHEDTDLDGTEEIYLQNGTVQAVLKLNSSASICELDSYPLKHNFADTLARQTEHYYRKIQPGLQGASSHQQSGIAS
AHDRVNFKHAISQADMEPDLYAQHLFIDRLNGELLDYQLHPSDKADEVIFQAITDFGSISKCFRIISNQLLVIYVCSGEA
SGYMQTEINLAMPSCDGPGGRYIRNHTVLSGFGQPIELDNLTAITLEDDTLGGSLSLDISPVTKFYACPHFTVSQSEGGF
EKIMQAVKLILTWKITDDQQQHITLRFDEMNTTV
>Mature_673_residues
TSSVSLLFGVHAHQPIGNFPEVVIDAHERCYKPFLQTLYRYPEFRFAIHFSGWLLDFLFEHYPKDMALLREMVTRGQIEI
FGAGDTEPVLAAIPERDRIGQLKTFSAKLKKKLGQSPQGAWLTERVWESTIVPALVNCGIHYVIVDDYHFICTGKNKIEL
DGYFSTEEDHHKLDLFPISEALRYRLPFSPALEAIAYIESLIDQSTTEHQPAAVYFDDIEKFGIWPETYQWVYEHGWLEK
FIQGVLASPHIQLQHYRDYHASEKTRGIVYLPTTSYIEMNEWTLPAEPAHIYDDLVQQAKSTGQYEQTRSFLRGGMWKNF
FSRYPESNWMHKRMLGLSARYASLPARQRTSAMQRCLYASQANDAYWHGLFGGLYLPHLRRTVYNNLIELEAQLDQCESR
NIYYHEDTDLDGTEEIYLQNGTVQAVLKLNSSASICELDSYPLKHNFADTLARQTEHYYRKIQPGLQGASSHQQSGIASA
HDRVNFKHAISQADMEPDLYAQHLFIDRLNGELLDYQLHPSDKADEVIFQAITDFGSISKCFRIISNQLLVIYVCSGEAS
GYMQTEINLAMPSCDGPGGRYIRNHTVLSGFGQPIELDNLTAITLEDDTLGGSLSLDISPVTKFYACPHFTVSQSEGGFE
KIMQAVKLILTWKITDDQQQHITLRFDEMNTTV

Specific function: Displays a broad range of substrate specificity, with the capacity to hydrolyze carbohydrates as simple as maltotriose [H]

COG id: COG1449

COG function: function code G; Alpha-amylase/alpha-mannosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 57 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015179
- InterPro:   IPR015178
- InterPro:   IPR011013
- InterPro:   IPR011330
- InterPro:   IPR000602
- InterPro:   IPR004300
- ProDom:   PD014405 [H]

Pfam domain/function: PF09094 DUF1925; PF09095 DUF1926; PF03065 Glyco_hydro_57 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 77178; Mature: 77047

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSVSLLFGVHAHQPIGNFPEVVIDAHERCYKPFLQTLYRYPEFRFAIHFSGWLLDFLF
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHH
EHYPKDMALLREMVTRGQIEIFGAGDTEPVLAAIPERDRIGQLKTFSAKLKKKLGQSPQG
HHCCHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
AWLTERVWESTIVPALVNCGIHYVIVDDYHFICTGKNKIELDGYFSTEEDHHKLDLFPIS
CHHHHHHHHHHHHHHHHHCCEEEEEEECEEEEEECCCEEEECCEECCCCCCCEEEEEEHH
EALRYRLPFSPALEAIAYIESLIDQSTTEHQPAAVYFDDIEKFGIWPETYQWVYEHGWLE
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHH
KFIQGVLASPHIQLQHYRDYHASEKTRGIVYLPTTSYIEMNEWTLPAEPAHIYDDLVQQA
HHHHHHHCCCCEEEEHHHHHCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHHH
KSTGQYEQTRSFLRGGMWKNFFSRYPESNWMHKRMLGLSARYASLPARQRTSAMQRCLYA
HHCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SQANDAYWHGLFGGLYLPHLRRTVYNNLIELEAQLDQCESRNIYYHEDTDLDGTEEIYLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEE
NGTVQAVLKLNSSASICELDSYPLKHNFADTLARQTEHYYRKIQPGLQGASSHQQSGIAS
CCCEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCH
AHDRVNFKHAISQADMEPDLYAQHLFIDRLNGELLDYQLHPSDKADEVIFQAITDFGSIS
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KCFRIISNQLLVIYVCSGEASGYMQTEINLAMPSCDGPGGRYIRNHTVLSGFGQPIELDN
HHHHHHCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEECCEEECCCCCCEEECC
LTAITLEDDTLGGSLSLDISPVTKFYACPHFTVSQSEGGFEKIMQAVKLILTWKITDDQQ
EEEEEEECCCCCCEEEEECCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHEEEEECCCCC
QHITLRFDEMNTTV
CEEEEEEECCCCCC
>Mature Secondary Structure 
TSSVSLLFGVHAHQPIGNFPEVVIDAHERCYKPFLQTLYRYPEFRFAIHFSGWLLDFLF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHH
EHYPKDMALLREMVTRGQIEIFGAGDTEPVLAAIPERDRIGQLKTFSAKLKKKLGQSPQG
HHCCHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
AWLTERVWESTIVPALVNCGIHYVIVDDYHFICTGKNKIELDGYFSTEEDHHKLDLFPIS
CHHHHHHHHHHHHHHHHHCCEEEEEEECEEEEEECCCEEEECCEECCCCCCCEEEEEEHH
EALRYRLPFSPALEAIAYIESLIDQSTTEHQPAAVYFDDIEKFGIWPETYQWVYEHGWLE
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHH
KFIQGVLASPHIQLQHYRDYHASEKTRGIVYLPTTSYIEMNEWTLPAEPAHIYDDLVQQA
HHHHHHHCCCCEEEEHHHHHCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHHH
KSTGQYEQTRSFLRGGMWKNFFSRYPESNWMHKRMLGLSARYASLPARQRTSAMQRCLYA
HHCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SQANDAYWHGLFGGLYLPHLRRTVYNNLIELEAQLDQCESRNIYYHEDTDLDGTEEIYLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEE
NGTVQAVLKLNSSASICELDSYPLKHNFADTLARQTEHYYRKIQPGLQGASSHQQSGIAS
CCCEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCH
AHDRVNFKHAISQADMEPDLYAQHLFIDRLNGELLDYQLHPSDKADEVIFQAITDFGSIS
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KCFRIISNQLLVIYVCSGEASGYMQTEINLAMPSCDGPGGRYIRNHTVLSGFGQPIELDN
HHHHHHCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEECCEEECCCCCCEEECC
LTAITLEDDTLGGSLSLDISPVTKFYACPHFTVSQSEGGFEKIMQAVKLILTWKITDDQQ
EEEEEEECCCCCCEEEEECCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHEEEEECCCCC
QHITLRFDEMNTTV
CEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226990; 8226989 [H]