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The map label for this gene is cysN [H]

Identifier: 114331187

GI number: 114331187

Start: 1241865

End: 1243148

Strand: Direct

Name: cysN [H]

Synonym: Neut_1189

Alternate gene names: 114331187

Gene position: 1241865-1243148 (Clockwise)

Preceding gene: 114331186

Following gene: 114331188

Centisome position: 46.67

GC content: 43.77

Gene sequence:

>1284_bases
ATGGCTGCTGTTGAAAATATTGAACCCGATCATGCCGAGCTGCTGCGATTTATTAGTGCAGGCAGTGTAGATGATGGTAA
AAGCACGCTGATAGGTCGTTTGCTGCATGATTCAAAATCCATCTTCGAAGATCAGTTGAGTGCTGTTACCAGAACCTCTA
ACAAACGCGGGATGGAAAAAATTGATCTCTCGCTTTTTACTGACGGATTGCAAGCAGAGCGGGAGCAGGGAATCACTATT
GATGTGGCGTATCGTTATTTCAATACACCCAAGCGCAAGTTTATTATTGCGGACACACCAGGCCATGAGCAATATACGCG
TAATATGGTAACGGGTGCTTCTACTGCCAATCTTGCAATTGTTCTTATCGATGCGCGCAAGGGAGTTTTGACACAATCCC
GGCGGCACGCCTACCTTGCCAGTCTGGTTGGCATTCCTCATCTGGTTGTGGCAATTAATAAAATGGATCTGGTTGGCTAT
TCCCAGGAAATCTTCAAGCAGATTTGTGCTGAATTTGCTGTGTTTTTACAAGGGCTGGGGTTTCGTTCCATTGAATATAT
ACCAATATCTGCGCTACTTGGAGATATGGTCGTGGAACGTGGTGACAACCTTGACTGGTATTCGGGCAAGCCATTATTGG
ATTATCTGGAATCCATTAATATAGAACACGATATCAACGTACTGGATTTTCGCTTTCCGGTACAGTGGGTATGTCGTCCG
CAAACGGAAGAAATGCATGATTTCCGTGGGTATACGGGTCGAATCGAATCCGGAACAATACGCGTGGGGGATGCAGTAAC
AGTATTACCATCCGGATTAACATCCCGCATCAAAGAAATTCTTTTATATAACGACAGTTTTGAAACCGCTTTTGCGCCAC
AATCCGTCATCTTGACCATTGAAGATCATCTGGATATCTCGCGCGGAGATATGCTAATCAATGGTGGAGAGCCGCCTCAA
ATTACCAGGGAGTTTGATGCCATGCTCTGCTGGTTATCCGAACAAGCATTTGACCCCAATCGTAAATATATTATCAAGCA
TACCACTCGTATCGTTAAAGGATTAATCACCCGAATTGACTATCGGGTTGATATAAATACAATGCATCAAGAGCCAGTTA
ACATTTTGACAATGAATGATATTGCTAGAATTGGAATGAAGGTACAGCAACCACTCGTCATTGATGCATATGCCAAAAAT
CGGGGAACTGGTTGTTTTATTCTGATTGATGAAGTCAGCAATAATACTGTGGCAGCCGGCATGATTTGTATCCGAGAAAG
CTGA

Upstream 100 bases:

>100_bases
GGCATCAATGGAGTTGCGTAAACGGGAAGGTTATTTCTAGGACAGTGTTCTGAAATAACACCCGGTTTTAATTTCTTATC
TTCATAGGTTTTTCTAGGAC

Downstream 100 bases:

>100_bases
TTACATTTTCCAAACCGAAAATTATTCCGATCAATAAATACCTGCCATCTGTTTATTCCTATTTTTGTATTAGAAAAATA
TCCTGGATATTTGACTAAAA

Product: sulfate adenylyltransferase, large subunit

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAAVENIEPDHAELLRFISAGSVDDGKSTLIGRLLHDSKSIFEDQLSAVTRTSNKRGMEKIDLSLFTDGLQAEREQGITI
DVAYRYFNTPKRKFIIADTPGHEQYTRNMVTGASTANLAIVLIDARKGVLTQSRRHAYLASLVGIPHLVVAINKMDLVGY
SQEIFKQICAEFAVFLQGLGFRSIEYIPISALLGDMVVERGDNLDWYSGKPLLDYLESINIEHDINVLDFRFPVQWVCRP
QTEEMHDFRGYTGRIESGTIRVGDAVTVLPSGLTSRIKEILLYNDSFETAFAPQSVILTIEDHLDISRGDMLINGGEPPQ
ITREFDAMLCWLSEQAFDPNRKYIIKHTTRIVKGLITRIDYRVDINTMHQEPVNILTMNDIARIGMKVQQPLVIDAYAKN
RGTGCFILIDEVSNNTVAAGMICIRES

Sequences:

>Translated_427_residues
MAAVENIEPDHAELLRFISAGSVDDGKSTLIGRLLHDSKSIFEDQLSAVTRTSNKRGMEKIDLSLFTDGLQAEREQGITI
DVAYRYFNTPKRKFIIADTPGHEQYTRNMVTGASTANLAIVLIDARKGVLTQSRRHAYLASLVGIPHLVVAINKMDLVGY
SQEIFKQICAEFAVFLQGLGFRSIEYIPISALLGDMVVERGDNLDWYSGKPLLDYLESINIEHDINVLDFRFPVQWVCRP
QTEEMHDFRGYTGRIESGTIRVGDAVTVLPSGLTSRIKEILLYNDSFETAFAPQSVILTIEDHLDISRGDMLINGGEPPQ
ITREFDAMLCWLSEQAFDPNRKYIIKHTTRIVKGLITRIDYRVDINTMHQEPVNILTMNDIARIGMKVQQPLVIDAYAKN
RGTGCFILIDEVSNNTVAAGMICIRES
>Mature_426_residues
AAVENIEPDHAELLRFISAGSVDDGKSTLIGRLLHDSKSIFEDQLSAVTRTSNKRGMEKIDLSLFTDGLQAEREQGITID
VAYRYFNTPKRKFIIADTPGHEQYTRNMVTGASTANLAIVLIDARKGVLTQSRRHAYLASLVGIPHLVVAINKMDLVGYS
QEIFKQICAEFAVFLQGLGFRSIEYIPISALLGDMVVERGDNLDWYSGKPLLDYLESINIEHDINVLDFRFPVQWVCRPQ
TEEMHDFRGYTGRIESGTIRVGDAVTVLPSGLTSRIKEILLYNDSFETAFAPQSVILTIEDHLDISRGDMLINGGEPPQI
TREFDAMLCWLSEQAFDPNRKYIIKHTTRIVKGLITRIDYRVDINTMHQEPVNILTMNDIARIGMKVQQPLVIDAYAKNR
GTGCFILIDEVSNNTVAAGMICIRES

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=28.4382284382284, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI5729864, Length=429, Percent_Identity=28.4382284382284, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4503475, Length=437, Percent_Identity=26.7734553775744, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4503471, Length=437, Percent_Identity=26.5446224256293, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194097354, Length=291, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI194018520, Length=291, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI194018522, Length=291, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=32.7645051194539, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI34147630, Length=417, Percent_Identity=25.1798561151079, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789108, Length=419, Percent_Identity=50.8353221957041, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI2367247, Length=317, Percent_Identity=27.1293375394322, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790412, Length=137, Percent_Identity=32.8467153284672, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789737, Length=137, Percent_Identity=32.8467153284672, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI32566629, Length=433, Percent_Identity=27.9445727482679, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI115532067, Length=315, Percent_Identity=29.8412698412698, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI115532065, Length=315, Percent_Identity=29.8412698412698, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17552884, Length=437, Percent_Identity=27.0022883295195, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=437, Percent_Identity=27.0022883295195, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=25.8139534883721, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25141371, Length=262, Percent_Identity=29.7709923664122, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=136, Percent_Identity=35.2941176470588, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=415, Percent_Identity=26.9879518072289, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=435, Percent_Identity=27.5862068965517, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=435, Percent_Identity=27.5862068965517, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6322937, Length=275, Percent_Identity=34.5454545454545, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=443, Percent_Identity=29.3453724604966, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324761, Length=285, Percent_Identity=27.3684210526316, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550900, Length=443, Percent_Identity=26.6365688487585, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=437, Percent_Identity=25.858123569794, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17137572, Length=437, Percent_Identity=25.858123569794, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45553807, Length=443, Percent_Identity=25.9593679458239, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553816, Length=443, Percent_Identity=25.9593679458239, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24651721, Length=443, Percent_Identity=25.9593679458239, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17864154, Length=443, Percent_Identity=25.9593679458239, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17137380, Length=439, Percent_Identity=27.3348519362187, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19921738, Length=175, Percent_Identity=33.7142857142857, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281363316, Length=251, Percent_Identity=28.2868525896414, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17864358, Length=251, Percent_Identity=28.2868525896414, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 47962; Mature: 47830

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVENIEPDHAELLRFISAGSVDDGKSTLIGRLLHDSKSIFEDQLSAVTRTSNKRGMEK
CCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
IDLSLFTDGLQAEREQGITIDVAYRYFNTPKRKFIIADTPGHEQYTRNMVTGASTANLAI
HHHHHHHCCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEE
VLIDARKGVLTQSRRHAYLASLVGIPHLVVAINKMDLVGYSQEIFKQICAEFAVFLQGLG
EEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEECCCHHHHHHHHHHHHHHHHHCCC
FRSIEYIPISALLGDMVVERGDNLDWYSGKPLLDYLESINIEHDINVLDFRFPVQWVCRP
CCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEECC
QTEEMHDFRGYTGRIESGTIRVGDAVTVLPSGLTSRIKEILLYNDSFETAFAPQSVILTI
CHHHHHHHCCCCCEECCCEEEECCEEEECCCCHHHHHHHHHEECCCCCCCCCCCEEEEEE
EDHLDISRGDMLINGGEPPQITREFDAMLCWLSEQAFDPNRKYIIKHTTRIVKGLITRID
ECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHE
YRVDINTMHQEPVNILTMNDIARIGMKVQQPLVIDAYAKNRGTGCFILIDEVSNNTVAAG
EEEEEECCCCCCCEEEEHHHHHHHCHHCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEE
MICIRES
EEEEECC
>Mature Secondary Structure 
AAVENIEPDHAELLRFISAGSVDDGKSTLIGRLLHDSKSIFEDQLSAVTRTSNKRGMEK
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
IDLSLFTDGLQAEREQGITIDVAYRYFNTPKRKFIIADTPGHEQYTRNMVTGASTANLAI
HHHHHHHCCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEE
VLIDARKGVLTQSRRHAYLASLVGIPHLVVAINKMDLVGYSQEIFKQICAEFAVFLQGLG
EEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEECCCHHHHHHHHHHHHHHHHHCCC
FRSIEYIPISALLGDMVVERGDNLDWYSGKPLLDYLESINIEHDINVLDFRFPVQWVCRP
CCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEECC
QTEEMHDFRGYTGRIESGTIRVGDAVTVLPSGLTSRIKEILLYNDSFETAFAPQSVILTI
CHHHHHHHCCCCCEECCCEEEECCEEEECCCCHHHHHHHHHEECCCCCCCCCCCEEEEEE
EDHLDISRGDMLINGGEPPQITREFDAMLCWLSEQAFDPNRKYIIKHTTRIVKGLITRID
ECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHE
YRVDINTMHQEPVNILTMNDIARIGMKVQQPLVIDAYAKNRGTGCFILIDEVSNNTVAAG
EEEEEECCCCCCCEEEEHHHHHHHCHHCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEE
MICIRES
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA