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The map label for this gene is folC [H]

Identifier: 114331153

GI number: 114331153

Start: 1195022

End: 1196308

Strand: Direct

Name: folC [H]

Synonym: Neut_1155

Alternate gene names: 114331153

Gene position: 1195022-1196308 (Clockwise)

Preceding gene: 114331152

Following gene: 114331154

Centisome position: 44.91

GC content: 46.7

Gene sequence:

>1287_bases
ATGATGGATGTCCGATCAATGACTTTAGATGACTGGTTGCTCTATCTTGAAAAACTTCATCCAAAGGCAATTGATATGGG
TCTGGAGCGGGTGAAGAAAGTAAGAGATGCTTTGGGATTGATGCCCAAATTTCCTGTTATTACGGTTGCTGGTACAAATG
GCAAAGGCTCAGTTTGTACCATACTCGAATCGATACTTGGTCGTGCATCCTACCGGGTAGGTTGTTATACATCACCACAT
TTACTGCATTATGGCGAGCGAATACGGATTGATCAGCGAGCAATTACTGACGAGGTTTTGTGTGGTGTATTTGCTGAGAT
AGAATCTGTACGTGAATCAGTCCAGGTTACGCTGACTTATTTTGAATTTGGTACCTTGGCCGCCATGCTGATATTTGTGC
GGGCGCAAGTGGATGTGGCCATTCTGGAAGTGGGATTAGGCGGCCGTCTTGATGCAGTTAATGTATTTGATACGGATTGC
GCCATCCTGACCAGTATCGACCTGGATCACATGGATTATCTTGGATCTACTCGGGAAGCCATTGGCCAGGAAAAAATTGG
TATCTTCAGGCCAAATAAACCGGCAATTTGTACTGAACCGGATATACCAGCCGGTCTTCTTCCAAAAATGAAGGCGACGG
GAGCCTTACTATGTTGCATCAACAAAAACTTCGGCTATACACAGGATAATCTGCAATGGTGCTATGAAGGAGTGCGCCGC
TATAACTTGTCATTGCCAGCGCTTAAAGGGGAATACCAGCTGCAAAATGCAAGTGCTGCTCTGGCAGCGCTGGAGGCTAT
GGAAGGAACATTGCCTGTTCCAATGAATGCCATACGACGTGGCCTGGCTGAGGCAACCCTGGCGGGTAGATTTCAGATAA
TTTCAGCAAGGCCTGTGATTATTCTGGACGTGGCGCATAATCCGGCAGCCACTCGCAAACTACTGGCTAATCTGGAAGCT
ATGCCAGTAAAAGGATGTACTTATGCAATCGTGGGCATGCTGAAAGATAAGGATATGGCTGGCACGATCCGGGTGCTGAA
GAATAATGTGGATCAGTGGTTGATAGCAGGTCTGGATGTGGCAAGAGGCGCAACAGGGAATGAAATACTAAAGGCACTTG
GAGATGCCGGAATAGCAGAACAGGAGTGCGTACGTGTATTTCAGAATGTACAGAGTGCCTATGTTTACGCGCGTGAACAT
GCCGGTAGTGATGATAGAATATGCGTTTTTGGATCGTTTCATACCGTCAGCTCGATATTGAAGAATCATGGCGGGACGGT
TTCTTAA

Upstream 100 bases:

>100_bases
TTACCTTTTGAATAACGGTGGTGTTAACTAAAAGGGATAATGTGAATCTACTACTTGCTGTCATGGTGAGCGTCTCTTTT
ACTTTATTTCTTCCGGATCG

Downstream 100 bases:

>100_bases
AGGTAGACAGGTATAAAAAATGACCAGCAGCGTCAATGAAGAAGAGATTTCCCTGAGGAAGCGTGCCAGAAAGCGCCTGA
TCGGTGCTATCTTGCTCGTT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MMDVRSMTLDDWLLYLEKLHPKAIDMGLERVKKVRDALGLMPKFPVITVAGTNGKGSVCTILESILGRASYRVGCYTSPH
LLHYGERIRIDQRAITDEVLCGVFAEIESVRESVQVTLTYFEFGTLAAMLIFVRAQVDVAILEVGLGGRLDAVNVFDTDC
AILTSIDLDHMDYLGSTREAIGQEKIGIFRPNKPAICTEPDIPAGLLPKMKATGALLCCINKNFGYTQDNLQWCYEGVRR
YNLSLPALKGEYQLQNASAALAALEAMEGTLPVPMNAIRRGLAEATLAGRFQIISARPVIILDVAHNPAATRKLLANLEA
MPVKGCTYAIVGMLKDKDMAGTIRVLKNNVDQWLIAGLDVARGATGNEILKALGDAGIAEQECVRVFQNVQSAYVYAREH
AGSDDRICVFGSFHTVSSILKNHGGTVS

Sequences:

>Translated_428_residues
MMDVRSMTLDDWLLYLEKLHPKAIDMGLERVKKVRDALGLMPKFPVITVAGTNGKGSVCTILESILGRASYRVGCYTSPH
LLHYGERIRIDQRAITDEVLCGVFAEIESVRESVQVTLTYFEFGTLAAMLIFVRAQVDVAILEVGLGGRLDAVNVFDTDC
AILTSIDLDHMDYLGSTREAIGQEKIGIFRPNKPAICTEPDIPAGLLPKMKATGALLCCINKNFGYTQDNLQWCYEGVRR
YNLSLPALKGEYQLQNASAALAALEAMEGTLPVPMNAIRRGLAEATLAGRFQIISARPVIILDVAHNPAATRKLLANLEA
MPVKGCTYAIVGMLKDKDMAGTIRVLKNNVDQWLIAGLDVARGATGNEILKALGDAGIAEQECVRVFQNVQSAYVYAREH
AGSDDRICVFGSFHTVSSILKNHGGTVS
>Mature_428_residues
MMDVRSMTLDDWLLYLEKLHPKAIDMGLERVKKVRDALGLMPKFPVITVAGTNGKGSVCTILESILGRASYRVGCYTSPH
LLHYGERIRIDQRAITDEVLCGVFAEIESVRESVQVTLTYFEFGTLAAMLIFVRAQVDVAILEVGLGGRLDAVNVFDTDC
AILTSIDLDHMDYLGSTREAIGQEKIGIFRPNKPAICTEPDIPAGLLPKMKATGALLCCINKNFGYTQDNLQWCYEGVRR
YNLSLPALKGEYQLQNASAALAALEAMEGTLPVPMNAIRRGLAEATLAGRFQIISARPVIILDVAHNPAATRKLLANLEA
MPVKGCTYAIVGMLKDKDMAGTIRVLKNNVDQWLIAGLDVARGATGNEILKALGDAGIAEQECVRVFQNVQSAYVYAREH
AGSDDRICVFGSFHTVSSILKNHGGTVS

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=375, Percent_Identity=28.2666666666667, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI66932984, Length=376, Percent_Identity=28.1914893617021, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788654, Length=416, Percent_Identity=45.9134615384615, Blast_Score=347, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI71984923, Length=469, Percent_Identity=28.3582089552239, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=469, Percent_Identity=28.3582089552239, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553150, Length=469, Percent_Identity=28.3582089552239, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=432, Percent_Identity=29.8611111111111, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=181, Percent_Identity=34.8066298342541, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24641571, Length=279, Percent_Identity=30.1075268817204, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24581568, Length=148, Percent_Identity=37.8378378378378, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46651; Mature: 46651

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDVRSMTLDDWLLYLEKLHPKAIDMGLERVKKVRDALGLMPKFPVITVAGTNGKGSVCT
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
ILESILGRASYRVGCYTSPHLLHYGERIRIDQRAITDEVLCGVFAEIESVRESVQVTLTY
HHHHHHCCCCEEEECCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
FEFGTLAAMLIFVRAQVDVAILEVGLGGRLDAVNVFDTDCAILTSIDLDHMDYLGSTREA
HHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCEEEEEECCHHHHHHHCCHHHH
IGQEKIGIFRPNKPAICTEPDIPAGLLPKMKATGALLCCINKNFGYTQDNLQWCYEGVRR
HCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
YNLSLPALKGEYQLQNASAALAALEAMEGTLPVPMNAIRRGLAEATLAGRFQIISARPVI
CCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCEE
ILDVAHNPAATRKLLANLEAMPVKGCTYAIVGMLKDKDMAGTIRVLKNNVDQWLIAGLDV
EEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
ARGATGNEILKALGDAGIAEQECVRVFQNVQSAYVYAREHAGSDDRICVFGSFHTVSSIL
HCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHH
KNHGGTVS
HHCCCCCC
>Mature Secondary Structure
MMDVRSMTLDDWLLYLEKLHPKAIDMGLERVKKVRDALGLMPKFPVITVAGTNGKGSVCT
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
ILESILGRASYRVGCYTSPHLLHYGERIRIDQRAITDEVLCGVFAEIESVRESVQVTLTY
HHHHHHCCCCEEEECCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
FEFGTLAAMLIFVRAQVDVAILEVGLGGRLDAVNVFDTDCAILTSIDLDHMDYLGSTREA
HHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCEEEEEECCHHHHHHHCCHHHH
IGQEKIGIFRPNKPAICTEPDIPAGLLPKMKATGALLCCINKNFGYTQDNLQWCYEGVRR
HCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
YNLSLPALKGEYQLQNASAALAALEAMEGTLPVPMNAIRRGLAEATLAGRFQIISARPVI
CCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCEE
ILDVAHNPAATRKLLANLEAMPVKGCTYAIVGMLKDKDMAGTIRVLKNNVDQWLIAGLDV
EEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
ARGATGNEILKALGDAGIAEQECVRVFQNVQSAYVYAREHAGSDDRICVFGSFHTVSSIL
HCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHH
KNHGGTVS
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]