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The map label for this gene is trpA [H]

Identifier: 114331151

GI number: 114331151

Start: 1193177

End: 1194001

Strand: Direct

Name: trpA [H]

Synonym: Neut_1153

Alternate gene names: 114331151

Gene position: 1193177-1194001 (Clockwise)

Preceding gene: 114331150

Following gene: 114331152

Centisome position: 44.84

GC content: 48.36

Gene sequence:

>825_bases
ATGAATCGTATTGAATCTGTTTTTTCCCGGCTCAAGTCACAAGATAAAGCTGCACTTATTCCTTTTATCACGGCAGGTGA
CCCTGACTCAGCCACAACAGTGGCGCTGATGCACCAGCTGACACGATCCGGGGTTGATTTGATTGAGCTTGGAGTACCTT
TTTCTGATCCCATGGCCGATGGGCCTACCATACAGCGTTCCTCGGAGCGCGCACTCAAACACCATATCAGCCTGAAAGAT
GTACTTGCTATGGTGGCACAATTCAGAAAAATGAACCAAAGCACACCGGTTGTCCTGATGGGTTATGCCAATCCAATTGA
GGCCATGGGGTATAAACGCTTTGTGGAAGAAGCAAGCCGTTCCGGGGTAGATGGCGTCCTGGTAGTTGATTATCCACCAG
AGGAGAGCGCTGAATGGGTGCGACACCTGAAAGATCAGGGCATCGATCCAATTTTTTTACTATCACCTACAACCCCGGAA
TCACGTGTCAAGCATGTTGCAGAACTTGCAAAAGGATATGTTTATTATGTTTCTCTAAAAGGTGTAACGGGTGCTTCACA
CCTTGATCTGCAGGATGTCAGCAGTAAATTGTCTCAGCTTCGTGCGCATATCAGGATTCCGATCGGGGTGGGTTTTGGCA
TACGAGACGAACAGACAGCCAGGAGCATAGCTGAGCGGGCGGATGCTGTAGTTATAGGTAGCCGTATTGTGGAGGAAATT
GAACACTCCCAGGCAGCTGATTTGCTGGCAAATGTAGGTGCTCTGGTAGAATCGTTGCGACATGCAATTGATGCTAGATC
CAATCACTCTTCTGACATAGGGTAG

Upstream 100 bases:

>100_bases
GACAAGGATATGGCAACAGTGGCACAGCAATCAGGTATTTCGCTTTAACCGAATGTCTGATAGATCTCATTATATTTTGA
TTTTTCGGATTATCTGTTAA

Downstream 100 bases:

>100_bases
TGATATGAGCTGGTTTGAAAGTATCATTCCCCCTAAAATCAAGCGTAAGGAAAACGGCGAAAAAAAAGCTGTCCCGGAAG
GCTTGTGGAGCAAATGTGCA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNRIESVFSRLKSQDKAALIPFITAGDPDSATTVALMHQLTRSGVDLIELGVPFSDPMADGPTIQRSSERALKHHISLKD
VLAMVAQFRKMNQSTPVVLMGYANPIEAMGYKRFVEEASRSGVDGVLVVDYPPEESAEWVRHLKDQGIDPIFLLSPTTPE
SRVKHVAELAKGYVYYVSLKGVTGASHLDLQDVSSKLSQLRAHIRIPIGVGFGIRDEQTARSIAERADAVVIGSRIVEEI
EHSQAADLLANVGALVESLRHAIDARSNHSSDIG

Sequences:

>Translated_274_residues
MNRIESVFSRLKSQDKAALIPFITAGDPDSATTVALMHQLTRSGVDLIELGVPFSDPMADGPTIQRSSERALKHHISLKD
VLAMVAQFRKMNQSTPVVLMGYANPIEAMGYKRFVEEASRSGVDGVLVVDYPPEESAEWVRHLKDQGIDPIFLLSPTTPE
SRVKHVAELAKGYVYYVSLKGVTGASHLDLQDVSSKLSQLRAHIRIPIGVGFGIRDEQTARSIAERADAVVIGSRIVEEI
EHSQAADLLANVGALVESLRHAIDARSNHSSDIG
>Mature_274_residues
MNRIESVFSRLKSQDKAALIPFITAGDPDSATTVALMHQLTRSGVDLIELGVPFSDPMADGPTIQRSSERALKHHISLKD
VLAMVAQFRKMNQSTPVVLMGYANPIEAMGYKRFVEEASRSGVDGVLVVDYPPEESAEWVRHLKDQGIDPIFLLSPTTPE
SRVKHVAELAKGYVYYVSLKGVTGASHLDLQDVSSKLSQLRAHIRIPIGVGFGIRDEQTARSIAERADAVVIGSRIVEEI
EHSQAADLLANVGALVESLRHAIDARSNHSSDIG

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=265, Percent_Identity=33.5849056603774, Blast_Score=157, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6321412, Length=236, Percent_Identity=38.9830508474576, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000909
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29857; Mature: 29857

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50007 PIPLC_X_DOMAIN ; PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIESVFSRLKSQDKAALIPFITAGDPDSATTVALMHQLTRSGVDLIELGVPFSDPMAD
CCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALKHHISLKDVLAMVAQFRKMNQSTPVVLMGYANPIEAMGYKRFVEEASR
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
SGVDGVLVVDYPPEESAEWVRHLKDQGIDPIFLLSPTTPESRVKHVAELAKGYVYYVSLK
CCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
GVTGASHLDLQDVSSKLSQLRAHIRIPIGVGFGIRDEQTARSIAERADAVVIGSRIVEEI
CCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
EHSQAADLLANVGALVESLRHAIDARSNHSSDIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNRIESVFSRLKSQDKAALIPFITAGDPDSATTVALMHQLTRSGVDLIELGVPFSDPMAD
CCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALKHHISLKDVLAMVAQFRKMNQSTPVVLMGYANPIEAMGYKRFVEEASR
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
SGVDGVLVVDYPPEESAEWVRHLKDQGIDPIFLLSPTTPESRVKHVAELAKGYVYYVSLK
CCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
GVTGASHLDLQDVSSKLSQLRAHIRIPIGVGFGIRDEQTARSIAERADAVVIGSRIVEEI
CCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
EHSQAADLLANVGALVESLRHAIDARSNHSSDIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]