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The map label for this gene is leuC [H]

Identifier: 114331142

GI number: 114331142

Start: 1183271

End: 1184683

Strand: Direct

Name: leuC [H]

Synonym: Neut_1144

Alternate gene names: 114331142

Gene position: 1183271-1184683 (Clockwise)

Preceding gene: 114331141

Following gene: 114331143

Centisome position: 44.47

GC content: 47.49

Gene sequence:

>1413_bases
ATGAAAACGTTATACGACAAGCTTTGGAATGGTCATGTAGTTCATGCTGAATCGGATGATCCAAATGGCATGGTGATACT
CTACATTGATCGCCACTTGGTGCACGAAGTAACCAGCCCTCAGGCTTTTGAAAATTTGAAGCTATCGGGACGCAGGCCGT
GGCGTACTGATTCTATCCTGGCGGTGGCGGATCACAATGTCCCTACAACTGACCGGAGTAGTGGTATCAGTGACCCGATA
TCTCGTTTACAGGTCGAAACACTAAGTCAAAATTGTGAGGAATTTGCAATTACCGAGTTCAAGATGGATGATGAACGTCA
GGGAATTGTACATGTGATCGGGCCGGAACAGGGCGCAACACTACCCGGCATGACTGTTGTCTGTGGGGATTCTCATACCA
GTACTCATGGGGCGTTTGCGTGTTTAGCGTTCGGCATTGGCACATCTGAGGTTGAGCATGTTTTAGCGACACAATGTCTG
GTTGCCCGAAAATCCAAAACAATGTTGGTCAAAGTTGAAGGAAATCTTGGTCAGGGTGTAACAGCTAAGGATATTGCATT
GGCTATTATCGGTGAAATAGGAACAGCGGGTGGTACAGGACACGCTATCGAGTTTGCTGGTAATGCGATTCGATCCCTAT
CCATGGAGGGCCGGATGACACTTTGCAATATGGCGATCGAGGCAGGCGCACGTGCGGGGATGATTGGAGTGGATGACGTC
ACGATCAATTATATTAAGGGAAGACCTTTTGCGCCTCGGAAAGATTTATGGGATCGGGCGGTTACGTATTGGCGTACATT
GAAAAGTGATGAGGATGCTGTTTTTGATCATATTGTGGAATTAAAGGCAGAGAACATCAAGCCTCAGGTAACCTGGGGAA
CTTCACCGGAAATGGTAACCACGATTGATGGATATGTGCCAGACCCTGCAGATATCAGTGATCCGATCAAGCGTCATAAT
ATTGAACATGCGCTTGAATATATGGGACTTAAGCCAGGAACGCCCATACAGGACATAGCACTGGATAAAATATTTATTGG
TTCTTGCACCAATTCCCGTATAGAAGATTTGCGTGCAGCAGCAGAAATTGTCAAAGGAAAACGGATTGCCCCTAATATTA
AGCTGGCTATGGTGGTTCCTGGTTCTGGTCTGGTCAAATCGATGGCTGAAACAGAGGGGCTGGATAGAATTTTTCAAGCG
GCAGGATTTGAATGGCGTGAACCTGGATGTTCAATGTGCCTGGCGATGAATGATGACAAATTATTGCCTGGAGAGCGCTG
TGCCTCGACTTCCAATCGAAATTTTGAGGGGCGGCAAGGCCCTGGCGGCCGAACGCACCTGGTTAGCCCATCTATGGCTG
CTGCGGCTGCCATATCTGGTCATTTTGTTGATGTACGCTCTTTTGCCCATTAA

Upstream 100 bases:

>100_bases
AAATTTACTGCCGGGAGATTTTTCATTTGCCCGGTAATTTGTTAATTAGACAGGCTTTATGGGATAATTACGCCTGTAAA
AACAGGTAATCATATTGAAA

Downstream 100 bases:

>100_bases
ACAGAGTTTATTATTTATATAGGAGATTGGTGATGAAAACACTGTTCGCATTATCTTTGCTGCTGATAATTACTGCGCAG
CTGACTGCTTGTAATACGAT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKTLYDKLWNGHVVHAESDDPNGMVILYIDRHLVHEVTSPQAFENLKLSGRRPWRTDSILAVADHNVPTTDRSSGISDPI
SRLQVETLSQNCEEFAITEFKMDDERQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAFGIGTSEVEHVLATQCL
VARKSKTMLVKVEGNLGQGVTAKDIALAIIGEIGTAGGTGHAIEFAGNAIRSLSMEGRMTLCNMAIEAGARAGMIGVDDV
TINYIKGRPFAPRKDLWDRAVTYWRTLKSDEDAVFDHIVELKAENIKPQVTWGTSPEMVTTIDGYVPDPADISDPIKRHN
IEHALEYMGLKPGTPIQDIALDKIFIGSCTNSRIEDLRAAAEIVKGKRIAPNIKLAMVVPGSGLVKSMAETEGLDRIFQA
AGFEWREPGCSMCLAMNDDKLLPGERCASTSNRNFEGRQGPGGRTHLVSPSMAAAAAISGHFVDVRSFAH

Sequences:

>Translated_470_residues
MKTLYDKLWNGHVVHAESDDPNGMVILYIDRHLVHEVTSPQAFENLKLSGRRPWRTDSILAVADHNVPTTDRSSGISDPI
SRLQVETLSQNCEEFAITEFKMDDERQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAFGIGTSEVEHVLATQCL
VARKSKTMLVKVEGNLGQGVTAKDIALAIIGEIGTAGGTGHAIEFAGNAIRSLSMEGRMTLCNMAIEAGARAGMIGVDDV
TINYIKGRPFAPRKDLWDRAVTYWRTLKSDEDAVFDHIVELKAENIKPQVTWGTSPEMVTTIDGYVPDPADISDPIKRHN
IEHALEYMGLKPGTPIQDIALDKIFIGSCTNSRIEDLRAAAEIVKGKRIAPNIKLAMVVPGSGLVKSMAETEGLDRIFQA
AGFEWREPGCSMCLAMNDDKLLPGERCASTSNRNFEGRQGPGGRTHLVSPSMAAAAAISGHFVDVRSFAH
>Mature_470_residues
MKTLYDKLWNGHVVHAESDDPNGMVILYIDRHLVHEVTSPQAFENLKLSGRRPWRTDSILAVADHNVPTTDRSSGISDPI
SRLQVETLSQNCEEFAITEFKMDDERQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAFGIGTSEVEHVLATQCL
VARKSKTMLVKVEGNLGQGVTAKDIALAIIGEIGTAGGTGHAIEFAGNAIRSLSMEGRMTLCNMAIEAGARAGMIGVDDV
TINYIKGRPFAPRKDLWDRAVTYWRTLKSDEDAVFDHIVELKAENIKPQVTWGTSPEMVTTIDGYVPDPADISDPIKRHN
IEHALEYMGLKPGTPIQDIALDKIFIGSCTNSRIEDLRAAAEIVKGKRIAPNIKLAMVVPGSGLVKSMAETEGLDRIFQA
AGFEWREPGCSMCLAMNDDKLLPGERCASTSNRNFEGRQGPGGRTHLVSPSMAAAAAISGHFVDVRSFAH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=361, Percent_Identity=27.7008310249307, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8659555, Length=397, Percent_Identity=26.7002518891688, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=25.5434782608696, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=62.3931623931624, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=25.7617728531856, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081781, Length=350, Percent_Identity=23.4285714285714, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367097, Length=343, Percent_Identity=24.4897959183673, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=363, Percent_Identity=29.4765840220386, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=29.4765840220386, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=29.1803278688525, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=403, Percent_Identity=26.0545905707196, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=63.1578947368421, Blast_Score=608, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6320440, Length=431, Percent_Identity=27.1461716937355, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=28.7292817679558, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=363, Percent_Identity=27.8236914600551, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281365315, Length=384, Percent_Identity=27.0833333333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864292, Length=384, Percent_Identity=27.0833333333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=27.0833333333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=474, Percent_Identity=24.6835443037975, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645686, Length=372, Percent_Identity=27.4193548387097, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137564, Length=406, Percent_Identity=26.1083743842365, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50911; Mature: 50911

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLYDKLWNGHVVHAESDDPNGMVILYIDRHLVHEVTSPQAFENLKLSGRRPWRTDSIL
CCCHHHHHCCCEEEEECCCCCCCEEEEEECHHHHHHCCCCHHHHCCCCCCCCCCCCCCEE
AVADHNVPTTDRSSGISDPISRLQVETLSQNCEEFAITEFKMDDERQGIVHVIGPEQGAT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFACLAFGIGTSEVEHVLATQCLVARKSKTMLVKVEGNLGQGV
CCCEEEEECCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
TAKDIALAIIGEIGTAGGTGHAIEFAGNAIRSLSMEGRMTLCNMAIEAGARAGMIGVDDV
CHHHHHEEEECCCCCCCCCCCEEECCCHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCE
TINYIKGRPFAPRKDLWDRAVTYWRTLKSDEDAVFDHIVELKAENIKPQVTWGTSPEMVT
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEE
TIDGYVPDPADISDPIKRHNIEHALEYMGLKPGTPIQDIALDKIFIGSCTNSRIEDLRAA
EECCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCHHHHHHCEEEEECCCCHHHHHHHHH
AEIVKGKRIAPNIKLAMVVPGSGLVKSMAETEGLDRIFQAAGFEWREPGCSMCLAMNDDK
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LLPGERCASTSNRNFEGRQGPGGRTHLVSPSMAAAAAISGHFVDVRSFAH
CCCCHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEHHHHCC
>Mature Secondary Structure
MKTLYDKLWNGHVVHAESDDPNGMVILYIDRHLVHEVTSPQAFENLKLSGRRPWRTDSIL
CCCHHHHHCCCEEEEECCCCCCCEEEEEECHHHHHHCCCCHHHHCCCCCCCCCCCCCCEE
AVADHNVPTTDRSSGISDPISRLQVETLSQNCEEFAITEFKMDDERQGIVHVIGPEQGAT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFACLAFGIGTSEVEHVLATQCLVARKSKTMLVKVEGNLGQGV
CCCEEEEECCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
TAKDIALAIIGEIGTAGGTGHAIEFAGNAIRSLSMEGRMTLCNMAIEAGARAGMIGVDDV
CHHHHHEEEECCCCCCCCCCCEEECCCHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCE
TINYIKGRPFAPRKDLWDRAVTYWRTLKSDEDAVFDHIVELKAENIKPQVTWGTSPEMVT
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEE
TIDGYVPDPADISDPIKRHNIEHALEYMGLKPGTPIQDIALDKIFIGSCTNSRIEDLRAA
EECCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCHHHHHHCEEEEECCCCHHHHHHHHH
AEIVKGKRIAPNIKLAMVVPGSGLVKSMAETEGLDRIFQAAGFEWREPGCSMCLAMNDDK
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LLPGERCASTSNRNFEGRQGPGGRTHLVSPSMAAAAAISGHFVDVRSFAH
CCCCHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]