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The map label for this gene is rpoH [H]

Identifier: 114331030

GI number: 114331030

Start: 1065284

End: 1066126

Strand: Direct

Name: rpoH [H]

Synonym: Neut_1031

Alternate gene names: 114331030

Gene position: 1065284-1066126 (Clockwise)

Preceding gene: 114331023

Following gene: 114331032

Centisome position: 40.03

GC content: 47.81

Gene sequence:

>843_bases
ATGAGTGAGATTTTAACTTTACCGGTAATAAGTGGAGGGAATCTGGAGAGCTATATTCGTGCAGCTAACAGTTTTCCTGT
TTTATCACGGGAAGAGGAAATATCGCTGGCTAAAAAAATGCAGGAAAATGGGGATATCAATGCAGCGCATCAACTGGTAC
TTTCTCACTTGCGTACAGTTGTGATGATTGCACGTGGTTATCTGGGCTATGGGTTGCCGCATGCTGATTTGATACAAGAA
GGTAATATCGGCTTGATGAAGGCTGTCAGACGTTTTGATCCGGAACGCGGTGTTCGCCTGATATCCTTTGCAATTCACTG
GATCAAGGCGGAAATCCATGAATTCATTATGCGCAACTGGAGGTTGGTCAAGATTGCCACCACCAAACCGCAGAGAAAGC
TTTTTTTCAATCTGCGCAGCATGAAAGAAGGGCTGGATACGATGAATCAGGCAGAGGTTGCGACCATGGCAGAGCAACTG
GGCGTTAAGGCGGAAGAGGTTGTCGAGATGGAAACACGCTTTAGTGGTCGGGATATTTCGCTTGAGCCGCTAACGGACAG
TGATGAAGAGGATTTCAGCCCGATTGCTTATCTGACAGATGGATCAGACCCGTCCAGAGAGCTGGAAAAAGAACAGCTGG
AGCGGTTGACGCATGCGAAACTGAAAGATTCTCTAGATAGCCTGGATGAGCGCAGTCGTCGTATTATTGAGGCCCGCTGG
CTGCGGGAAGATAAGGCCGCAACCCTGCATGAACTGGCTAAAGAATTGGGTGTTTCTGCCGAACGGGTGCGCCAGATTGA
AGCTAAGGCCATGAAGAAAATGCGAGCTGAAATAGCTATTTAA

Upstream 100 bases:

>100_bases
CCTTAATATTGAATTAGCACTCGAGGGGTGAGAGTGCTAAAATATGCTTGTTGATTTTATATTCCGTAATATTCATTCGG
AAATCAGGAGGAAAATATAT

Downstream 100 bases:

>100_bases
CAACTGCTTTATGTAATCTCCTGATAGATGGCCGTATTGCTTGCGGCCATCAATTTTTTAATGCAGTTATCCATTTTTTG
TACAGACGATCTGCAACTGA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSEILTLPVISGGNLESYIRAANSFPVLSREEEISLAKKMQENGDINAAHQLVLSHLRTVVMIARGYLGYGLPHADLIQE
GNIGLMKAVRRFDPERGVRLISFAIHWIKAEIHEFIMRNWRLVKIATTKPQRKLFFNLRSMKEGLDTMNQAEVATMAEQL
GVKAEEVVEMETRFSGRDISLEPLTDSDEEDFSPIAYLTDGSDPSRELEKEQLERLTHAKLKDSLDSLDERSRRIIEARW
LREDKAATLHELAKELGVSAERVRQIEAKAMKKMRAEIAI

Sequences:

>Translated_280_residues
MSEILTLPVISGGNLESYIRAANSFPVLSREEEISLAKKMQENGDINAAHQLVLSHLRTVVMIARGYLGYGLPHADLIQE
GNIGLMKAVRRFDPERGVRLISFAIHWIKAEIHEFIMRNWRLVKIATTKPQRKLFFNLRSMKEGLDTMNQAEVATMAEQL
GVKAEEVVEMETRFSGRDISLEPLTDSDEEDFSPIAYLTDGSDPSRELEKEQLERLTHAKLKDSLDSLDERSRRIIEARW
LREDKAATLHELAKELGVSAERVRQIEAKAMKKMRAEIAI
>Mature_279_residues
SEILTLPVISGGNLESYIRAANSFPVLSREEEISLAKKMQENGDINAAHQLVLSHLRTVVMIARGYLGYGLPHADLIQEG
NIGLMKAVRRFDPERGVRLISFAIHWIKAEIHEFIMRNWRLVKIATTKPQRKLFFNLRSMKEGLDTMNQAEVATMAEQLG
VKAEEVVEMETRFSGRDISLEPLTDSDEEDFSPIAYLTDGSDPSRELEKEQLERLTHAKLKDSLDSLDERSRRIIEARWL
REDKAATLHELAKELGVSAERVRQIEAKAMKKMRAEIAI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=57.6086956521739, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1789098, Length=262, Percent_Identity=33.206106870229, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789448, Length=230, Percent_Identity=30.8695652173913, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31881; Mature: 31750

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEILTLPVISGGNLESYIRAANSFPVLSREEEISLAKKMQENGDINAAHQLVLSHLRTV
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VMIARGYLGYGLPHADLIQEGNIGLMKAVRRFDPERGVRLISFAIHWIKAEIHEFIMRNW
HHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLVKIATTKPQRKLFFNLRSMKEGLDTMNQAEVATMAEQLGVKAEEVVEMETRFSGRDIS
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCE
LEPLTDSDEEDFSPIAYLTDGSDPSRELEKEQLERLTHAKLKDSLDSLDERSRRIIEARW
ECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREDKAATLHELAKELGVSAERVRQIEAKAMKKMRAEIAI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEILTLPVISGGNLESYIRAANSFPVLSREEEISLAKKMQENGDINAAHQLVLSHLRTV
CCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VMIARGYLGYGLPHADLIQEGNIGLMKAVRRFDPERGVRLISFAIHWIKAEIHEFIMRNW
HHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLVKIATTKPQRKLFFNLRSMKEGLDTMNQAEVATMAEQLGVKAEEVVEMETRFSGRDIS
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCE
LEPLTDSDEEDFSPIAYLTDGSDPSRELEKEQLERLTHAKLKDSLDSLDERSRRIIEARW
ECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREDKAATLHELAKELGVSAERVRQIEAKAMKKMRAEIAI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]