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The map label for this gene is sbp [H]

Identifier: 114331028

GI number: 114331028

Start: 1060558

End: 1061565

Strand: Reverse

Name: sbp [H]

Synonym: Neut_1028

Alternate gene names: 114331028

Gene position: 1061565-1060558 (Counterclockwise)

Preceding gene: 114331029

Following gene: 114331027

Centisome position: 39.89

GC content: 48.02

Gene sequence:

>1008_bases
ATGCAATTCAACACATGGTTGTCAGTCGGTCTATTAATATTGGTTATGGTAAATTCTAATCAGGCACTTGCTGATCGGGC
CATTCTCAATGTTTCCTATGATCCGACACGCGAGCTGTATGAGGAATATAATCGTAAATTCACCCGCTACTGGCAGGAAA
AAACCGGAGAGAAAATTACTGTCAGACAGTCTCATGGCGGCTCCGGCAAGCAGGCACGTGCGGTCATCGATGGTGTGCCT
GCAGATGTAGTGACACTTGCCCTTGCTCACGACATCGATGCAATCAGCGAACAATCCGGACTGATCCTGGCCGAGTGGCA
GAAACGCTTATCCAACAACAGCTCACCCTATCTCTCAACAATCGTCCTGCTGGTACGCAAAGATAATCCCAAAGGAATCA
AGGACTGGGATGATTTGATCAAACCGGATGTCGCCGTCGTAACCCCCAACCCGAAAACATCGGGTGGCGCACGCTGGAAT
TATCTGGCAGCGTGGGGTTTTGCACTAAAAAAACATGATGGTGATGAAGCAAAAGCACAAGAATTCCTCACCAGACTTTA
TAAGAATGTCTCGATACTCGATTCAGGGGCGCGTGGATCAACCATCAACTTCATCCAGCGCGGTATAGGGGACGTTCTGA
TTTCCTGGGAAAATGAAGCTTTTCTGGCACTCAAGGAATATGGCCCGGAGAAATTCGAAATAATCATGCCTTCGATCAGT
ATTCTTGCCGAACCCCCTGTCACCGTCGTTGACAAAAATGCTGACAGACATGGCGTACGTAATATCGCGCAGGCCTATCT
CGAGTATCTATACAGCAAAGAGGGACAGGAAATTGCTGCACGCAACTTCTATCGGCCGAGCGATCCGGAAATAGCCAGGA
AATACGCTCACCAGTTTCCGGCAATCGATCTGTTTACGATAAATGAAGTATTTGGTGGCTGGCCGAAAGCGCAGAGCATC
CACTTCAAGGATGGCGGCCTGTTCGACAAAATATATGTAAATCAATGA

Upstream 100 bases:

>100_bases
GCGGAACTAGGAGAAACAACTGCCGGTACGGACTTTTTTTATGTAGAAATCGCTGTCAGCGCATTCAAATAACATCGACT
TATTTACAAAAGGAAATCAA

Downstream 100 bases:

>100_bases
TTGATTCTGTATCCAGCATATTGACTGCTCATAAATTCAAATTCAAGAAGACCCGGAAATGTCAGATTTTTCTGAGTGTA
CACCCACGCCTATTGAGTAG

Product: sulfate ABC transporter, periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MQFNTWLSVGLLILVMVNSNQALADRAILNVSYDPTRELYEEYNRKFTRYWQEKTGEKITVRQSHGGSGKQARAVIDGVP
ADVVTLALAHDIDAISEQSGLILAEWQKRLSNNSSPYLSTIVLLVRKDNPKGIKDWDDLIKPDVAVVTPNPKTSGGARWN
YLAAWGFALKKHDGDEAKAQEFLTRLYKNVSILDSGARGSTINFIQRGIGDVLISWENEAFLALKEYGPEKFEIIMPSIS
ILAEPPVTVVDKNADRHGVRNIAQAYLEYLYSKEGQEIAARNFYRPSDPEIARKYAHQFPAIDLFTINEVFGGWPKAQSI
HFKDGGLFDKIYVNQ

Sequences:

>Translated_335_residues
MQFNTWLSVGLLILVMVNSNQALADRAILNVSYDPTRELYEEYNRKFTRYWQEKTGEKITVRQSHGGSGKQARAVIDGVP
ADVVTLALAHDIDAISEQSGLILAEWQKRLSNNSSPYLSTIVLLVRKDNPKGIKDWDDLIKPDVAVVTPNPKTSGGARWN
YLAAWGFALKKHDGDEAKAQEFLTRLYKNVSILDSGARGSTINFIQRGIGDVLISWENEAFLALKEYGPEKFEIIMPSIS
ILAEPPVTVVDKNADRHGVRNIAQAYLEYLYSKEGQEIAARNFYRPSDPEIARKYAHQFPAIDLFTINEVFGGWPKAQSI
HFKDGGLFDKIYVNQ
>Mature_335_residues
MQFNTWLSVGLLILVMVNSNQALADRAILNVSYDPTRELYEEYNRKFTRYWQEKTGEKITVRQSHGGSGKQARAVIDGVP
ADVVTLALAHDIDAISEQSGLILAEWQKRLSNNSSPYLSTIVLLVRKDNPKGIKDWDDLIKPDVAVVTPNPKTSGGARWN
YLAAWGFALKKHDGDEAKAQEFLTRLYKNVSILDSGARGSTINFIQRGIGDVLISWENEAFLALKEYGPEKFEIIMPSIS
ILAEPPVTVVDKNADRHGVRNIAQAYLEYLYSKEGQEIAARNFYRPSDPEIARKYAHQFPAIDLFTINEVFGGWPKAQSI
HFKDGGLFDKIYVNQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=329, Percent_Identity=63.5258358662614, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1788765, Length=305, Percent_Identity=46.8852459016393, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37693; Mature: 37693

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFNTWLSVGLLILVMVNSNQALADRAILNVSYDPTRELYEEYNRKFTRYWQEKTGEKIT
CCCHHHHHHHHHEEEEECCCCHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VRQSHGGSGKQARAVIDGVPADVVTLALAHDIDAISEQSGLILAEWQKRLSNNSSPYLST
EEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHEE
IVLLVRKDNPKGIKDWDDLIKPDVAVVTPNPKTSGGARWNYLAAWGFALKKHDGDEAKAQ
EEEEEECCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCHHHHH
EFLTRLYKNVSILDSGARGSTINFIQRGIGDVLISWENEAFLALKEYGPEKFEIIMPSIS
HHHHHHHCCCCEEECCCCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEEECCHH
ILAEPPVTVVDKNADRHGVRNIAQAYLEYLYSKEGQEIAARNFYRPSDPEIARKYAHQFP
HHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHCC
AIDLFTINEVFGGWPKAQSIHFKDGGLFDKIYVNQ
CEEEEEHHHHHCCCCCCCEEEECCCCCEEEEEECC
>Mature Secondary Structure
MQFNTWLSVGLLILVMVNSNQALADRAILNVSYDPTRELYEEYNRKFTRYWQEKTGEKIT
CCCHHHHHHHHHEEEEECCCCHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VRQSHGGSGKQARAVIDGVPADVVTLALAHDIDAISEQSGLILAEWQKRLSNNSSPYLST
EEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHEE
IVLLVRKDNPKGIKDWDDLIKPDVAVVTPNPKTSGGARWNYLAAWGFALKKHDGDEAKAQ
EEEEEECCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCHHHHH
EFLTRLYKNVSILDSGARGSTINFIQRGIGDVLISWENEAFLALKEYGPEKFEIIMPSIS
HHHHHHHCCCCEEECCCCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEEECCHH
ILAEPPVTVVDKNADRHGVRNIAQAYLEYLYSKEGQEIAARNFYRPSDPEIARKYAHQFP
HHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHCC
AIDLFTINEVFGGWPKAQSIHFKDGGLFDKIYVNQ
CEEEEEHHHHHCCCCCCCEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]