Click here to switch to the map view.

The map label for this gene is rlmE

Identifier: 114330999

GI number: 114330999

Start: 1026443

End: 1027063

Strand: Direct

Name: rlmE

Synonym: Neut_0997

Alternate gene names: 114330999

Gene position: 1026443-1027063 (Clockwise)

Preceding gene: 114330995

Following gene: 114331000

Centisome position: 38.57

GC content: 47.5

Gene sequence:

>621_bases
ATGAAATCTGCCAGAACCAGCAGAGCCTGGATAAAAGCGCATATCAACGATAACTTTGTCCGCAAGGCTAACCACGAAGG
TTATCGTTCGCGTGCGGCCTACAAATTACAGGAAATCGCCGAGCGCGATGCACTTTTTTCACCGGGAATGACGGTTGTTG
ATCTGGGAGCGTCGCCTGGCAGCTGGTCACAAGTGGCACTCGAATCTGTCGGGCCGACCGGAAAGGTTTTTGCGCTGGAT
ATGCTGGATATGCAATCCTTACCTGGTGCGACATTCATTCAAGGCGATTTTCGTGAAAATGGTGTTCTGGCCGCATTGGA
GAAAGTTCTGGATGGGAAACGGGCAGATCTTGTAATTTCCGATATGTCACCCAATCTTACCGGTATTCGTGTCAGTGATC
AGGCTCACGGCATGTATCTGGCAGAACTTGCTTTGATGTTCTGTCGCGAACATTTGAACCCCGGTGAAAACTTTCTTGTC
AAAGTATTTCAGGGGAGTGACTTTGAGGCATTCCGACAATTAATGCAGGCTGATTTTGCAAAAGTCGTCATCAGGAAACC
AAAGGCTTCGCGAGATCGAAGTAAAGAGTTATATTTGCTGGGACTGGAAAAAATAATCTGA

Upstream 100 bases:

>100_bases
CTACCGGATTCAGGTGGTGCGCACTGGCCGTAAGCTGCCGGCGCAGAGCCATATCAAGTTCAAGCATTACAACCTTCCTA
AAAACACTGGATTTTACCTG

Downstream 100 bases:

>100_bases
TAGACTGCGGTAACAGTCCTGTAACAGATACAGATAAACCGCAGCGTTTCAAAAATTCGGTTTCGTCGAGAAGCAAAATT
GAGGAGCTAATATGAATAAT

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKSARTSRAWIKAHINDNFVRKANHEGYRSRAAYKLQEIAERDALFSPGMTVVDLGASPGSWSQVALESVGPTGKVFALD
MLDMQSLPGATFIQGDFRENGVLAALEKVLDGKRADLVISDMSPNLTGIRVSDQAHGMYLAELALMFCREHLNPGENFLV
KVFQGSDFEAFRQLMQADFAKVVIRKPKASRDRSKELYLLGLEKII

Sequences:

>Translated_206_residues
MKSARTSRAWIKAHINDNFVRKANHEGYRSRAAYKLQEIAERDALFSPGMTVVDLGASPGSWSQVALESVGPTGKVFALD
MLDMQSLPGATFIQGDFRENGVLAALEKVLDGKRADLVISDMSPNLTGIRVSDQAHGMYLAELALMFCREHLNPGENFLV
KVFQGSDFEAFRQLMQADFAKVVIRKPKASRDRSKELYLLGLEKII
>Mature_206_residues
MKSARTSRAWIKAHINDNFVRKANHEGYRSRAAYKLQEIAERDALFSPGMTVVDLGASPGSWSQVALESVGPTGKVFALD
MLDMQSLPGATFIQGDFRENGVLAALEKVLDGKRADLVISDMSPNLTGIRVSDQAHGMYLAELALMFCREHLNPGENFLV
KVFQGSDFEAFRQLMQADFAKVVIRKPKASRDRSKELYLLGLEKII

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=203, Percent_Identity=38.423645320197, Blast_Score=144, Evalue=5e-35,
Organism=Homo sapiens, GI29029591, Length=184, Percent_Identity=35.3260869565217, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI29029589, Length=184, Percent_Identity=35.3260869565217, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI7110661, Length=184, Percent_Identity=35.3260869565217, Blast_Score=110, Evalue=6e-25,
Organism=Homo sapiens, GI194097365, Length=184, Percent_Identity=30.9782608695652, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1789569, Length=199, Percent_Identity=46.2311557788945, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17553474, Length=204, Percent_Identity=32.3529411764706, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17554650, Length=190, Percent_Identity=32.6315789473684, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=184, Percent_Identity=31.5217391304348, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=184, Percent_Identity=31.5217391304348, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17553860, Length=184, Percent_Identity=31.5217391304348, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319535, Length=196, Percent_Identity=33.6734693877551, Blast_Score=102, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=185, Percent_Identity=29.7297297297297, Blast_Score=88, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321302, Length=97, Percent_Identity=31.9587628865979, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356387, Length=207, Percent_Identity=35.2657004830918, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647580, Length=196, Percent_Identity=31.6326530612245, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI18859957, Length=184, Percent_Identity=29.8913043478261, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648639, Length=195, Percent_Identity=29.7435897435897, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_NITEC (Q0AHC6)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747221.1
- ProteinModelPortal:   Q0AHC6
- SMR:   Q0AHC6
- STRING:   Q0AHC6
- GeneID:   4274149
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0997
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q0AHC6
- ProtClustDB:   CLSK585365
- BioCyc:   NEUT335283:NEUT_0997-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 22893; Mature: 22893

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 60-60 BINDING 62-62 BINDING 80-80 BINDING 96-96 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSARTSRAWIKAHINDNFVRKANHEGYRSRAAYKLQEIAERDALFSPGMTVVDLGASPG
CCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
SWSQVALESVGPTGKVFALDMLDMQSLPGATFIQGDFRENGVLAALEKVLDGKRADLVIS
CHHHHHHHHCCCCCCEEEEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEE
DMSPNLTGIRVSDQAHGMYLAELALMFCREHLNPGENFLVKVFQGSDFEAFRQLMQADFA
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
KVVIRKPKASRDRSKELYLLGLEKII
HHHHCCCCCCCCCCCEEEEEEHHHCC
>Mature Secondary Structure
MKSARTSRAWIKAHINDNFVRKANHEGYRSRAAYKLQEIAERDALFSPGMTVVDLGASPG
CCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
SWSQVALESVGPTGKVFALDMLDMQSLPGATFIQGDFRENGVLAALEKVLDGKRADLVIS
CHHHHHHHHCCCCCCEEEEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEE
DMSPNLTGIRVSDQAHGMYLAELALMFCREHLNPGENFLVKVFQGSDFEAFRQLMQADFA
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
KVVIRKPKASRDRSKELYLLGLEKII
HHHHCCCCCCCCCCCEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA