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The map label for this gene is asnB [H]

Identifier: 114330904

GI number: 114330904

Start: 922283

End: 924217

Strand: Direct

Name: asnB [H]

Synonym: Neut_0901

Alternate gene names: 114330904

Gene position: 922283-924217 (Clockwise)

Preceding gene: 114330903

Following gene: 114330905

Centisome position: 34.66

GC content: 48.68

Gene sequence:

>1935_bases
ATGTGCGGCATAACGGGGGTATTTGATACGCGCGGCGGGAGTGGGATTGATCGTAATCTGCTTCATTGTATGAATCAGAC
ACTGACACACCGTGGGCCGGATGAGGGGGAAATGCATGTTGAGCCTGGTGTTGGATTAGGACATCGTCGATTGTCGATCA
TGGATATATCCAGTGGCCAACAACCCTTATTCAATGAAGATGGCAGTGTGGTCGTCATATTCAATGGCGAAATCTATAAT
TTTCAGAAGCTGGTCAGTGAGTTGACAGCTTTGGGACATCAATTTCGTACGCATTGCGATACCGAGGTAATCGTGCACGC
CTGGGAGGAGTGGGGTGAACGCTGTGTTGAACGCTTTAGCGGGATGTTTGCCTTTGGCGTATGGGATCGTAATCAGCAAA
CGCTATTTATGGCACGTGATCGGCTGGGCATCAAACCGTTTTACTATACCTTGCTGGATAACGGATTGTTTCTGTTTGCC
TCGGAGCTCAAAGCATTGCTGGTTCACCCGGATTGTGACAAAACATTGGATCATCAGGCGATAGAAGATTATTTTGCCTA
CGGTTATATCCCCGAGCCGAAAACCATTTTTAAAAACACGTTTAAGCTGAGTCCCGGATATTCATTATTATTGCGTCAAG
GGCAGCAGTCAGCCTTGCCTCGTGAATACTGGGATATACCGTTTGCATCACATGGCGCATTGAGTGAGCAAGAAGCGGCA
GAAGAGCTGATTGTCCGGTTACGAAATGCAGTAGATAGTCACCTGATGAGCGAAGTGCCGCTGGGCGCATTTCTTTCCGG
CGGAGTGGATTCCAGTGCTGTCGTGGCGATGATGGGCGGCCTGATGAAGGAGCCTGTTAATACCTGTTCGATTTCTTTTG
CTGATCCTGCTTTTGATGAATCAGATTATGCTCGGCTGGTTGCGGATCGCTACCAGACCCACCATTTTACCGAGCAGGTC
AAACAGGATGATTTTGATCTCATTGACCGGTTGGCTACGCTGTACGATGAACCGTTTGCCGATAGTTCCGCCATCCCGAC
TTATCGTGTGTGTGAGCTGGCTAGAAAACAGGTGACGGTAGCCCTTTCAGGCGATGGAGGTGATGAGAATTTTGCCGGTT
ATCGCCGCTATCGCTGGCACATGATAGAAGAGCGGTTGCGTTCTAAATTACCATTAGGATTGCGAAAACCCTTGTTTGGT
CTGTTGGGTAATATTTATCCCAAAGCGGACTGGGCACCGAAGTTTTTGCGTGCCAAAACCACGTTTGAATCGCTTGCGCG
CGATTCTGTGGAAGGCTATTTTCACAGCGTATCCATTCTGGATAATAAACTGCGTAGGCAATTGTTTACTCGGGATTTTC
ACCATAGTCTGCGGGGTTATCAGGCAGTTGAGGTACTGCGTAGTTATGCGGACAAAAGCCCGACGCAAGATCCCTTGTCG
CTGATTCAGTATCTGGATATGAAAACCTATCTTGTAGGCGATATCCTGACTAAAGTGGATCGTGCCAGTATGGCGCATTC
ACTGGAGGTGCGCGTCCCGCTGCTGGATCATGAACTGGTCGAATGGGTTTCAGGATTGCCTGCCTCTATGAAATTACGCC
AACAGGAAGGAAAGTACATTCTGAAAAAGTCGCTGGAACCGTATTTGCCGCATGAAGTGCTTTATCGGAACAAGATGGGA
TTTGCCGTGCCGTTGGCCAGCTGGTTTCGCGGCCCATTGCGTGAACGTGTGCGTGCTGCACTGCTTGGTAATACGCTGGC
GAGCACAGGGATTTTTGATATGGTCTTCATCAAAAAGATGTTGGATCAGCATCAATCCGGCAGACGGGATTATAGTGCTC
CTATCTGGACATTGCTGATGTTTGAGGCTTTCTTGCGCAATACCCTCAATGTGGGAAATGTGTCATATACCGAACAAGGC
CAAAAGGTTGCCTGA

Upstream 100 bases:

>100_bases
CGAATGGCTATCTTGCAGTGTATGACCAGGTATTGGGTCATAATGCTGCTCACGATTAAAGATTGGAAAGATCAAAAAGA
TCAACTGACTGGATAGGAAT

Downstream 100 bases:

>100_bases
TATCAATTCAATGCAATACAGATTTCCCGGCCGGGATGCAGAATAGCTCAATGCTGAACGTCTACCTCACAGTTGATACC
GAGTTATGGCCTTATTCCGA

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MCGITGVFDTRGGSGIDRNLLHCMNQTLTHRGPDEGEMHVEPGVGLGHRRLSIMDISSGQQPLFNEDGSVVVIFNGEIYN
FQKLVSELTALGHQFRTHCDTEVIVHAWEEWGERCVERFSGMFAFGVWDRNQQTLFMARDRLGIKPFYYTLLDNGLFLFA
SELKALLVHPDCDKTLDHQAIEDYFAYGYIPEPKTIFKNTFKLSPGYSLLLRQGQQSALPREYWDIPFASHGALSEQEAA
EELIVRLRNAVDSHLMSEVPLGAFLSGGVDSSAVVAMMGGLMKEPVNTCSISFADPAFDESDYARLVADRYQTHHFTEQV
KQDDFDLIDRLATLYDEPFADSSAIPTYRVCELARKQVTVALSGDGGDENFAGYRRYRWHMIEERLRSKLPLGLRKPLFG
LLGNIYPKADWAPKFLRAKTTFESLARDSVEGYFHSVSILDNKLRRQLFTRDFHHSLRGYQAVEVLRSYADKSPTQDPLS
LIQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWVSGLPASMKLRQQEGKYILKKSLEPYLPHEVLYRNKMG
FAVPLASWFRGPLRERVRAALLGNTLASTGIFDMVFIKKMLDQHQSGRRDYSAPIWTLLMFEAFLRNTLNVGNVSYTEQG
QKVA

Sequences:

>Translated_644_residues
MCGITGVFDTRGGSGIDRNLLHCMNQTLTHRGPDEGEMHVEPGVGLGHRRLSIMDISSGQQPLFNEDGSVVVIFNGEIYN
FQKLVSELTALGHQFRTHCDTEVIVHAWEEWGERCVERFSGMFAFGVWDRNQQTLFMARDRLGIKPFYYTLLDNGLFLFA
SELKALLVHPDCDKTLDHQAIEDYFAYGYIPEPKTIFKNTFKLSPGYSLLLRQGQQSALPREYWDIPFASHGALSEQEAA
EELIVRLRNAVDSHLMSEVPLGAFLSGGVDSSAVVAMMGGLMKEPVNTCSISFADPAFDESDYARLVADRYQTHHFTEQV
KQDDFDLIDRLATLYDEPFADSSAIPTYRVCELARKQVTVALSGDGGDENFAGYRRYRWHMIEERLRSKLPLGLRKPLFG
LLGNIYPKADWAPKFLRAKTTFESLARDSVEGYFHSVSILDNKLRRQLFTRDFHHSLRGYQAVEVLRSYADKSPTQDPLS
LIQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWVSGLPASMKLRQQEGKYILKKSLEPYLPHEVLYRNKMG
FAVPLASWFRGPLRERVRAALLGNTLASTGIFDMVFIKKMLDQHQSGRRDYSAPIWTLLMFEAFLRNTLNVGNVSYTEQG
QKVA
>Mature_644_residues
MCGITGVFDTRGGSGIDRNLLHCMNQTLTHRGPDEGEMHVEPGVGLGHRRLSIMDISSGQQPLFNEDGSVVVIFNGEIYN
FQKLVSELTALGHQFRTHCDTEVIVHAWEEWGERCVERFSGMFAFGVWDRNQQTLFMARDRLGIKPFYYTLLDNGLFLFA
SELKALLVHPDCDKTLDHQAIEDYFAYGYIPEPKTIFKNTFKLSPGYSLLLRQGQQSALPREYWDIPFASHGALSEQEAA
EELIVRLRNAVDSHLMSEVPLGAFLSGGVDSSAVVAMMGGLMKEPVNTCSISFADPAFDESDYARLVADRYQTHHFTEQV
KQDDFDLIDRLATLYDEPFADSSAIPTYRVCELARKQVTVALSGDGGDENFAGYRRYRWHMIEERLRSKLPLGLRKPLFG
LLGNIYPKADWAPKFLRAKTTFESLARDSVEGYFHSVSILDNKLRRQLFTRDFHHSLRGYQAVEVLRSYADKSPTQDPLS
LIQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWVSGLPASMKLRQQEGKYILKKSLEPYLPHEVLYRNKMG
FAVPLASWFRGPLRERVRAALLGNTLASTGIFDMVFIKKMLDQHQSGRRDYSAPIWTLLMFEAFLRNTLNVGNVSYTEQG
QKVA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=395, Percent_Identity=27.3417721518987, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI168229252, Length=395, Percent_Identity=27.3417721518987, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI168229250, Length=395, Percent_Identity=27.3417721518987, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI296010848, Length=366, Percent_Identity=27.0491803278689, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI296010852, Length=296, Percent_Identity=26.6891891891892, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI296010850, Length=296, Percent_Identity=26.6891891891892, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786889, Length=391, Percent_Identity=30.690537084399, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71993933, Length=389, Percent_Identity=30.5912596401028, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25147557, Length=389, Percent_Identity=30.5912596401028, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25147560, Length=353, Percent_Identity=28.8951841359773, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17560178, Length=356, Percent_Identity=27.247191011236, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325403, Length=411, Percent_Identity=27.2506082725061, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321563, Length=413, Percent_Identity=26.1501210653753, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45553209, Length=402, Percent_Identity=29.1044776119403, Blast_Score=142, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 73188; Mature: 73188

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGVFDTRGGSGIDRNLLHCMNQTLTHRGPDEGEMHVEPGVGLGHRRLSIMDISSGQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPLFNEDGSVVVIFNGEIYNFQKLVSELTALGHQFRTHCDTEVIVHAWEEWGERCVERFS
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GMFAFGVWDRNQQTLFMARDRLGIKPFYYTLLDNGLFLFASELKALLVHPDCDKTLDHQA
CCEEEEEECCCCCEEEEEHHCCCCCHHHHHHHHCCHHHHHHHHHHEEECCCCCCHHHHHH
IEDYFAYGYIPEPKTIFKNTFKLSPGYSLLLRQGQQSALPREYWDIPFASHGALSEQEAA
HHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHCCHHHCCCHHHCCCCCCCCCCCCHHHHH
EELIVRLRNAVDSHLMSEVPLGAFLSGGVDSSAVVAMMGGLMKEPVNTCSISFADPAFDE
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
SDYARLVADRYQTHHFTEQVKQDDFDLIDRLATLYDEPFADSSAIPTYRVCELARKQVTV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEE
ALSGDGGDENFAGYRRYRWHMIEERLRSKLPLGLRKPLFGLLGNIYPKADWAPKFLRAKT
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
TFESLARDSVEGYFHSVSILDNKLRRQLFTRDFHHSLRGYQAVEVLRSYADKSPTQDPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LIQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWVSGLPASMKLRQQEGKYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCCHHHHHHHHHCHHH
LKKSLEPYLPHEVLYRNKMGFAVPLASWFRGPLRERVRAALLGNTLASTGIFDMVFIKKM
HHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQHQSGRRDYSAPIWTLLMFEAFLRNTLNVGNVSYTEQGQKVA
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGITGVFDTRGGSGIDRNLLHCMNQTLTHRGPDEGEMHVEPGVGLGHRRLSIMDISSGQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPLFNEDGSVVVIFNGEIYNFQKLVSELTALGHQFRTHCDTEVIVHAWEEWGERCVERFS
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GMFAFGVWDRNQQTLFMARDRLGIKPFYYTLLDNGLFLFASELKALLVHPDCDKTLDHQA
CCEEEEEECCCCCEEEEEHHCCCCCHHHHHHHHCCHHHHHHHHHHEEECCCCCCHHHHHH
IEDYFAYGYIPEPKTIFKNTFKLSPGYSLLLRQGQQSALPREYWDIPFASHGALSEQEAA
HHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHCCHHHCCCHHHCCCCCCCCCCCCHHHHH
EELIVRLRNAVDSHLMSEVPLGAFLSGGVDSSAVVAMMGGLMKEPVNTCSISFADPAFDE
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
SDYARLVADRYQTHHFTEQVKQDDFDLIDRLATLYDEPFADSSAIPTYRVCELARKQVTV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEE
ALSGDGGDENFAGYRRYRWHMIEERLRSKLPLGLRKPLFGLLGNIYPKADWAPKFLRAKT
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
TFESLARDSVEGYFHSVSILDNKLRRQLFTRDFHHSLRGYQAVEVLRSYADKSPTQDPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LIQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWVSGLPASMKLRQQEGKYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCCHHHHHHHHHCHHH
LKKSLEPYLPHEVLYRNKMGFAVPLASWFRGPLRERVRAALLGNTLASTGIFDMVFIKKM
HHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQHQSGRRDYSAPIWTLLMFEAFLRNTLNVGNVSYTEQGQKVA
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]