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The map label for this gene is ppsA [H]

Identifier: 114330874

GI number: 114330874

Start: 886409

End: 888739

Strand: Direct

Name: ppsA [H]

Synonym: Neut_0868

Alternate gene names: 114330874

Gene position: 886409-888739 (Clockwise)

Preceding gene: 114330873

Following gene: 114330875

Centisome position: 33.31

GC content: 51.14

Gene sequence:

>2331_bases
ATGAACGATGTGGCGAGCGTAGGGGGCAAGAATGCCTCGCTGGGGGAGATGATCGGCAATCTTTCCCGCGCCGGGGTGAA
AGTGCCTGGCGGATTTGCTACGACAACATACGCTTATCGTGAATTTCTCAGGATCAATGATTTGAATGATCGCATCAATC
AATTATTGACCCCACTGGATGTTGACGATGTGGAGGCGTTGGCCGTTGCGGGTAAACAGATTCGCTCTTGGTTGCTTGCC
GCCACGCTGCCGGATGATCTGGTAGAAGTAGTGACTCGGGCATACCAGAAGCTCATTGCTGATTCGGGTGAGAAAGTATC
CTTTGCAGTACGTTCATCGGCAACAGCTGAGGATCTAGCGGATGCCTCCTTTGCCGGCCAGCAGGAGACGCTGCTGAACG
TGCAGGGGCTTGATAACCTGTTCAGTGCACTCAGAAGAGTGTTTGCTTCACTTTATACGGACCGGGCGATTTCCTATCGG
ACCCATCACGGGTTTCATCATGATCAGGTATTTTTATCTGCGGGCGTACAACGGATGGCGCGCAGCGATAAAGGTGCCAG
TGGCGTGATATTTACACTTGATACTGAATCTGGCTTTCGGGATGTGGTGTTTATTACTTCTGCTTATGGTTTGGGGGAAA
CCGTCGTACAGGGAACAGTCAATCCGGATGAGTTTATTGTTTATAAACAAAATCTTAAGGTGGGATTACCCGCAATTCTT
TCCCGCCGGCTGGGAGCCAAGGCAACCGAGATGATCTATAGCGATGAGCAGGCAGGGCAGACGACCGTGGTACAGCCGGT
ATCGCCGGAACGAAGTCAGCGTTTCAGTTTGTCCGATGAGCAAATTGAATTATTGGCCCGACAGGCGGTGATCATCGAAC
AGCACTACAACCGGCCGATGGATATCGAATGGGCACTGGATGGAATAGATGGACAGATTTATATTGTCCAGGCCCGCCCG
GAAACGGTGAAAAGCCGTGCCGAGCAATCAGTAGAACAATTTCACCTGAGCGGACGAGGCCCGGTGCTGGTGCAGGGGCG
CGCGATCGGCCAGAAGATCGGACAGGGAACGGCGCGGGTCATTATGAGCGCGGGGCAGATGGCCGAGATTCAGCCGGGCG
ACGTGCTGGTAACGGACATGACAGATCCGGATTGGGAGCCAATCATGAAACGAGCCGCAGCGATTGTGACCAATCGTGGC
GGGCGTACCTGTCACGCTGCTATTGTTGCGCGCGAGCTGGGCATTCCTGCGGTGGTCGGGTGTGAGCTGGCTACGACAAA
AATCAGCGATGGCCAGGCGGTGACCATATCCTGTGCGGAAGGAGATACAGGTTTTGTTTATGAAGGTCTGCTTCCTTTTC
AGCATACTTGTTCAGAGATCAGTAAGCTGCCTGAATTACCAGTCAAGATCATGTTGAATATCGGAAATCCTGCGCAGGCT
TTTGCATTTTCACAACTGCCCAATCATGGAGTGGGGCTGGCACGTCTGGAATTTATCGTCGCCAACAATATTGGTATCCA
TCCCCGGGCCGTGCTGGAATTTGATGCGCTTCCGGATGATCTGAAAACATCTGTCAGTAAGCGAACGACAGGTTACCCCG
ACCCGGTGTCTTTCTATATTGAGAAACTGGTGGAAGGTATTGCCACGTTGGCGGCAGCTTTTCACCCACATCCGGTGATT
GTGCGTCTATCTGATTTTAAATCTAATGAATACGCGAATCTGATTGCGGGAGATCGCTTTGAACCACATGAGGAAAATCC
GATGATTGGATTTCGTGGTACTTCACGTTATCTTGCTGATTCTTTTCGTGCCTGCTTTGAGCTAGAGTGTCGGGCTTTGC
GCAAGGTACGTGATGAAATGGGTTTTACCAATGTTGAAGTGATGGTTCCTTTTTGTCGAACCCTGGAAGAGGCGCAGCAG
ATTACAGAATTACTGGCAGCTAATGGATTAAAACGCGGAAGCAATGGGCTACGTTTGATCATGATGTGCGAAATTCCTTC
CAATGCTGTGTTGGCTGAAGAATTCCTCGAATACTTCGATGGGTTTTCCATCGGGTCTAATGATATGACCCAGCTGGTAC
TTGGGCTGGATCGTGATTCGGAGCTGGTTGCTCATTTATTCGATGAGCGTAATCCAGCTGTGAAGCGGCTGCTGAAAATG
GCAATTGATGCTTGCCATAAACAGGGGAAATATATTGGTATTTGCGGCCAGGCACCCTCTGATTATCCGGATTTTGCACG
CTGGCTGGTTGAACAGGGTATCAGCAGCCTTTCTCTTAATCAGGACACGGTAGTGAGCACCTGGCTGCATCTGGCGGACC
GGGAGCAGTAG

Upstream 100 bases:

>100_bases
GACGATAGTGCACAGATGTTATCGTATCCGTTCTGTTTTTCAATGAGAGTAAGTTATGGCTGGTCATCCTGACTATATTT
TGTGGCTGGATCAGGTAGGT

Downstream 100 bases:

>100_bases
GGCGATGTTACAACGCAGCGTTTTTTTTCTGTCTGATCGTACGGGCATTACAGCGGAAACACTGGGTCATAGTCTGTTGA
CCCAGTTCGATGGAATAGAG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MNDVASVGGKNASLGEMIGNLSRAGVKVPGGFATTTYAYREFLRINDLNDRINQLLTPLDVDDVEALAVAGKQIRSWLLA
ATLPDDLVEVVTRAYQKLIADSGEKVSFAVRSSATAEDLADASFAGQQETLLNVQGLDNLFSALRRVFASLYTDRAISYR
THHGFHHDQVFLSAGVQRMARSDKGASGVIFTLDTESGFRDVVFITSAYGLGETVVQGTVNPDEFIVYKQNLKVGLPAIL
SRRLGAKATEMIYSDEQAGQTTVVQPVSPERSQRFSLSDEQIELLARQAVIIEQHYNRPMDIEWALDGIDGQIYIVQARP
ETVKSRAEQSVEQFHLSGRGPVLVQGRAIGQKIGQGTARVIMSAGQMAEIQPGDVLVTDMTDPDWEPIMKRAAAIVTNRG
GRTCHAAIVARELGIPAVVGCELATTKISDGQAVTISCAEGDTGFVYEGLLPFQHTCSEISKLPELPVKIMLNIGNPAQA
FAFSQLPNHGVGLARLEFIVANNIGIHPRAVLEFDALPDDLKTSVSKRTTGYPDPVSFYIEKLVEGIATLAAAFHPHPVI
VRLSDFKSNEYANLIAGDRFEPHEENPMIGFRGTSRYLADSFRACFELECRALRKVRDEMGFTNVEVMVPFCRTLEEAQQ
ITELLAANGLKRGSNGLRLIMMCEIPSNAVLAEEFLEYFDGFSIGSNDMTQLVLGLDRDSELVAHLFDERNPAVKRLLKM
AIDACHKQGKYIGICGQAPSDYPDFARWLVEQGISSLSLNQDTVVSTWLHLADREQ

Sequences:

>Translated_776_residues
MNDVASVGGKNASLGEMIGNLSRAGVKVPGGFATTTYAYREFLRINDLNDRINQLLTPLDVDDVEALAVAGKQIRSWLLA
ATLPDDLVEVVTRAYQKLIADSGEKVSFAVRSSATAEDLADASFAGQQETLLNVQGLDNLFSALRRVFASLYTDRAISYR
THHGFHHDQVFLSAGVQRMARSDKGASGVIFTLDTESGFRDVVFITSAYGLGETVVQGTVNPDEFIVYKQNLKVGLPAIL
SRRLGAKATEMIYSDEQAGQTTVVQPVSPERSQRFSLSDEQIELLARQAVIIEQHYNRPMDIEWALDGIDGQIYIVQARP
ETVKSRAEQSVEQFHLSGRGPVLVQGRAIGQKIGQGTARVIMSAGQMAEIQPGDVLVTDMTDPDWEPIMKRAAAIVTNRG
GRTCHAAIVARELGIPAVVGCELATTKISDGQAVTISCAEGDTGFVYEGLLPFQHTCSEISKLPELPVKIMLNIGNPAQA
FAFSQLPNHGVGLARLEFIVANNIGIHPRAVLEFDALPDDLKTSVSKRTTGYPDPVSFYIEKLVEGIATLAAAFHPHPVI
VRLSDFKSNEYANLIAGDRFEPHEENPMIGFRGTSRYLADSFRACFELECRALRKVRDEMGFTNVEVMVPFCRTLEEAQQ
ITELLAANGLKRGSNGLRLIMMCEIPSNAVLAEEFLEYFDGFSIGSNDMTQLVLGLDRDSELVAHLFDERNPAVKRLLKM
AIDACHKQGKYIGICGQAPSDYPDFARWLVEQGISSLSLNQDTVVSTWLHLADREQ
>Mature_776_residues
MNDVASVGGKNASLGEMIGNLSRAGVKVPGGFATTTYAYREFLRINDLNDRINQLLTPLDVDDVEALAVAGKQIRSWLLA
ATLPDDLVEVVTRAYQKLIADSGEKVSFAVRSSATAEDLADASFAGQQETLLNVQGLDNLFSALRRVFASLYTDRAISYR
THHGFHHDQVFLSAGVQRMARSDKGASGVIFTLDTESGFRDVVFITSAYGLGETVVQGTVNPDEFIVYKQNLKVGLPAIL
SRRLGAKATEMIYSDEQAGQTTVVQPVSPERSQRFSLSDEQIELLARQAVIIEQHYNRPMDIEWALDGIDGQIYIVQARP
ETVKSRAEQSVEQFHLSGRGPVLVQGRAIGQKIGQGTARVIMSAGQMAEIQPGDVLVTDMTDPDWEPIMKRAAAIVTNRG
GRTCHAAIVARELGIPAVVGCELATTKISDGQAVTISCAEGDTGFVYEGLLPFQHTCSEISKLPELPVKIMLNIGNPAQA
FAFSQLPNHGVGLARLEFIVANNIGIHPRAVLEFDALPDDLKTSVSKRTTGYPDPVSFYIEKLVEGIATLAAAFHPHPVI
VRLSDFKSNEYANLIAGDRFEPHEENPMIGFRGTSRYLADSFRACFELECRALRKVRDEMGFTNVEVMVPFCRTLEEAQQ
ITELLAANGLKRGSNGLRLIMMCEIPSNAVLAEEFLEYFDGFSIGSNDMTQLVLGLDRDSELVAHLFDERNPAVKRLLKM
AIDACHKQGKYIGICGQAPSDYPDFARWLVEQGISSLSLNQDTVVSTWLHLADREQ

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=774, Percent_Identity=62.1447028423773, Blast_Score=999, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=379, Percent_Identity=26.1213720316623, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788726, Length=395, Percent_Identity=25.0632911392405, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI48994992, Length=394, Percent_Identity=25.3807106598985, Blast_Score=104, Evalue=3e-23,
Organism=Escherichia coli, GI1789193, Length=402, Percent_Identity=25.1243781094527, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=320, Percent_Identity=26.5625, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 85075; Mature: 85075

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVASVGGKNASLGEMIGNLSRAGVKVPGGFATTTYAYREFLRINDLNDRINQLLTPLD
CCCCHHCCCCCCHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
VDDVEALAVAGKQIRSWLLAATLPDDLVEVVTRAYQKLIADSGEKVSFAVRSSATAEDLA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
DASFAGQQETLLNVQGLDNLFSALRRVFASLYTDRAISYRTHHGFHHDQVFLSAGVQRMA
HHHCCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHH
RSDKGASGVIFTLDTESGFRDVVFITSAYGLGETVVQGTVNPDEFIVYKQNLKVGLPAIL
HCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHCCCCCCCCEEEEECCCCCCCHHHH
SRRLGAKATEMIYSDEQAGQTTVVQPVSPERSQRFSLSDEQIELLARQAVIIEQHYNRPM
HHHHCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DIEWALDGIDGQIYIVQARPETVKSRAEQSVEQFHLSGRGPVLVQGRAIGQKIGQGTARV
CEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCHHHH
IMSAGQMAEIQPGDVLVTDMTDPDWEPIMKRAAAIVTNRGGRTCHAAIVARELGIPAVVG
HHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEE
CELATTKISDGQAVTISCAEGDTGFVYEGLLPFQHTCSEISKLPELPVKIMLNIGNPAQA
CCEEEEEECCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHH
FAFSQLPNHGVGLARLEFIVANNIGIHPRAVLEFDALPDDLKTSVSKRTTGYPDPVSFYI
HHHHCCCCCCCCHHHEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHH
EKLVEGIATLAAAFHPHPVIVRLSDFKSNEYANLIAGDRFEPHEENPMIGFRGTSRYLAD
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCEEEECCCHHHHHH
SFRACFELECRALRKVRDEMGFTNVEVMVPFCRTLEEAQQITELLAANGLKRGSNGLRLI
HHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
MMCEIPSNAVLAEEFLEYFDGFSIGSNDMTQLVLGLDRDSELVAHLFDERNPAVKRLLKM
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
AIDACHKQGKYIGICGQAPSDYPDFARWLVEQGISSLSLNQDTVVSTWLHLADREQ
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDVASVGGKNASLGEMIGNLSRAGVKVPGGFATTTYAYREFLRINDLNDRINQLLTPLD
CCCCHHCCCCCCHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
VDDVEALAVAGKQIRSWLLAATLPDDLVEVVTRAYQKLIADSGEKVSFAVRSSATAEDLA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
DASFAGQQETLLNVQGLDNLFSALRRVFASLYTDRAISYRTHHGFHHDQVFLSAGVQRMA
HHHCCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHH
RSDKGASGVIFTLDTESGFRDVVFITSAYGLGETVVQGTVNPDEFIVYKQNLKVGLPAIL
HCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHCCCCCCCCEEEEECCCCCCCHHHH
SRRLGAKATEMIYSDEQAGQTTVVQPVSPERSQRFSLSDEQIELLARQAVIIEQHYNRPM
HHHHCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DIEWALDGIDGQIYIVQARPETVKSRAEQSVEQFHLSGRGPVLVQGRAIGQKIGQGTARV
CEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCHHHH
IMSAGQMAEIQPGDVLVTDMTDPDWEPIMKRAAAIVTNRGGRTCHAAIVARELGIPAVVG
HHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEE
CELATTKISDGQAVTISCAEGDTGFVYEGLLPFQHTCSEISKLPELPVKIMLNIGNPAQA
CCEEEEEECCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHH
FAFSQLPNHGVGLARLEFIVANNIGIHPRAVLEFDALPDDLKTSVSKRTTGYPDPVSFYI
HHHHCCCCCCCCHHHEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHH
EKLVEGIATLAAAFHPHPVIVRLSDFKSNEYANLIAGDRFEPHEENPMIGFRGTSRYLAD
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCEEEECCCHHHHHH
SFRACFELECRALRKVRDEMGFTNVEVMVPFCRTLEEAQQITELLAANGLKRGSNGLRLI
HHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
MMCEIPSNAVLAEEFLEYFDGFSIGSNDMTQLVLGLDRDSELVAHLFDERNPAVKRLLKM
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
AIDACHKQGKYIGICGQAPSDYPDFARWLVEQGISSLSLNQDTVVSTWLHLADREQ
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]