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The map label for this gene is ccsA [H]

Identifier: 114330867

GI number: 114330867

Start: 878993

End: 880276

Strand: Reverse

Name: ccsA [H]

Synonym: Neut_0861

Alternate gene names: 114330867

Gene position: 880276-878993 (Counterclockwise)

Preceding gene: 114330868

Following gene: 114330866

Centisome position: 33.08

GC content: 49.69

Gene sequence:

>1284_bases
ATGCCCCCACAAGATGTTGCCACTTTGTCACCGGGTGAAAACAAGAAAACGATTGAACTGCCCATTCTGTCCGGTAATGA
GGGTCCTGATGTTATTGATATTCGCTCGTTATACGCTCAAAGCAATATGTTCACCTACGATCCCGGGTTTGTATCCACCG
CCAGCTGCAAATCAGCCATTACATTCATCGATGGTGATCAAGGTATCCTGCTGTATCGCGGCTACCCGATCGAACAACTG
GCCACCCGCTGTGACTTCATGGAAGTCAGCTATCTGCTGCTGAATGGAGAATTGCCTACAGCAGACCAAAAAAAGCAATT
CGTCAATAACATCAAAAACCATACGATGCTGCATGAGCAAATGATCAAGTTTCTCAGCGGATTTCGCCGCGATGCACATC
CCATGGCGGTCATGGTCGGCGTGGTGGGTGCATTGTCTGCTTTTTATCATGACGCCATGGACGTTTCTGATGCGCCACAT
CGTGAACAATCCATATTTCGTCTGATTGCCAAACTACCAACCATTACGGCAATTGCCTATAAATATAATATCGGCCAGCC
TTTCATCTATCCTCGTAATGATCTGGGCTTTACGGAAAACTTCCTGCACATGATGTTTGCCACGCCAGCCGAGAAATATG
TTCCCAACCCGGTCATTGTCAGGGCACTGGATCGCATTCTGATTCTGCATGCGGATCACGAGCAAAATGCTTCCACATCA
ACAGTCCGTCTGGCGGGCTCAAGCGGTGCCAATCCATTTGCCTGCATCTCTGCCGGGATTACCTGCTTATGGGGTCCGGC
GCATGGCGGCGCGAATGAAGCCTGCCTCAATATGCTGGAAGAAATCGGAGACGTTTCACGCATCAATGAATACATTACCC
GCGCCAAGGACAAGAATGATCCTTTCCGGCTGATGGGCTTTGGCCATCGGGTCTACAAAAACTTTGATCCACGCGCCACA
CTGATGCGCGACACCTGCCACGAAGTACTGGATGAACTGGGGCTACATAATGACCGGCTGTTTAAGCTTGCTCTGGAGCT
GGAAAGAATCGCCCTGGAAGATGACTTTTTTATTACCAGAAAGCTGTACCCCAACGTTGACTTCTACTCGGGAATCGTAC
AACGGGCGCTGGGTATTCCAACATCCATGTTTACTGCCATTTTTGCTACCGCACGTACCGTTGGCTGGGTTGCGCAGTGG
AACGAGATGATCTCTGATCCGGAACAGAAAATAGGTAGACCCCGCCAATTATATGTTGGAGTCCCGCGCAGAGATGTACC
ATGA

Upstream 100 bases:

>100_bases
TGAAGCATTTCGGCCCATATTGAGACTTTTACAGGAAAATTAACCAAAACCCTGGTCATCTATCAGGAAAATTTGAATTG
CAGGATAAATGGAGAAAATT

Downstream 100 bases:

>100_bases
CAATCATGGCTATATCTGATCAACAGGAAATTTACAAACCTGGCGGCTACAGCTGGCAAGAATAAAAGACAAGCAGCAGA
TTACCCGTACCTCGCCATTT

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPPQDVATLSPGENKKTIELPILSGNEGPDVIDIRSLYAQSNMFTYDPGFVSTASCKSAITFIDGDQGILLYRGYPIEQL
ATRCDFMEVSYLLLNGELPTADQKKQFVNNIKNHTMLHEQMIKFLSGFRRDAHPMAVMVGVVGALSAFYHDAMDVSDAPH
REQSIFRLIAKLPTITAIAYKYNIGQPFIYPRNDLGFTENFLHMMFATPAEKYVPNPVIVRALDRILILHADHEQNASTS
TVRLAGSSGANPFACISAGITCLWGPAHGGANEACLNMLEEIGDVSRINEYITRAKDKNDPFRLMGFGHRVYKNFDPRAT
LMRDTCHEVLDELGLHNDRLFKLALELERIALEDDFFITRKLYPNVDFYSGIVQRALGIPTSMFTAIFATARTVGWVAQW
NEMISDPEQKIGRPRQLYVGVPRRDVP

Sequences:

>Translated_427_residues
MPPQDVATLSPGENKKTIELPILSGNEGPDVIDIRSLYAQSNMFTYDPGFVSTASCKSAITFIDGDQGILLYRGYPIEQL
ATRCDFMEVSYLLLNGELPTADQKKQFVNNIKNHTMLHEQMIKFLSGFRRDAHPMAVMVGVVGALSAFYHDAMDVSDAPH
REQSIFRLIAKLPTITAIAYKYNIGQPFIYPRNDLGFTENFLHMMFATPAEKYVPNPVIVRALDRILILHADHEQNASTS
TVRLAGSSGANPFACISAGITCLWGPAHGGANEACLNMLEEIGDVSRINEYITRAKDKNDPFRLMGFGHRVYKNFDPRAT
LMRDTCHEVLDELGLHNDRLFKLALELERIALEDDFFITRKLYPNVDFYSGIVQRALGIPTSMFTAIFATARTVGWVAQW
NEMISDPEQKIGRPRQLYVGVPRRDVP
>Mature_426_residues
PPQDVATLSPGENKKTIELPILSGNEGPDVIDIRSLYAQSNMFTYDPGFVSTASCKSAITFIDGDQGILLYRGYPIEQLA
TRCDFMEVSYLLLNGELPTADQKKQFVNNIKNHTMLHEQMIKFLSGFRRDAHPMAVMVGVVGALSAFYHDAMDVSDAPHR
EQSIFRLIAKLPTITAIAYKYNIGQPFIYPRNDLGFTENFLHMMFATPAEKYVPNPVIVRALDRILILHADHEQNASTST
VRLAGSSGANPFACISAGITCLWGPAHGGANEACLNMLEEIGDVSRINEYITRAKDKNDPFRLMGFGHRVYKNFDPRATL
MRDTCHEVLDELGLHNDRLFKLALELERIALEDDFFITRKLYPNVDFYSGIVQRALGIPTSMFTAIFATARTVGWVAQWN
EMISDPEQKIGRPRQLYVGVPRRDVP

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=391, Percent_Identity=27.8772378516624, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786939, Length=408, Percent_Identity=66.1764705882353, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1786527, Length=360, Percent_Identity=32.5, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=384, Percent_Identity=27.34375, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324328, Length=370, Percent_Identity=27.5675675675676, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=330, Percent_Identity=26.0606060606061, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=411, Percent_Identity=27.4939172749392, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24640126, Length=347, Percent_Identity=27.0893371757925, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640124, Length=398, Percent_Identity=25.6281407035176, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356863, Length=351, Percent_Identity=25.6410256410256, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47915; Mature: 47783

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPQDVATLSPGENKKTIELPILSGNEGPDVIDIRSLYAQSNMFTYDPGFVSTASCKSAI
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHCCCCEEECCCCCCHHHHHCEE
TFIDGDQGILLYRGYPIEQLATRCDFMEVSYLLLNGELPTADQKKQFVNNIKNHTMLHEQ
EEEECCCCEEEECCCCHHHHHHHCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
MIKFLSGFRRDAHPMAVMVGVVGALSAFYHDAMDVSDAPHREQSIFRLIAKLPTITAIAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEE
KYNIGQPFIYPRNDLGFTENFLHMMFATPAEKYVPNPVIVRALDRILILHADHEQNASTS
EEECCCCCEEECCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHEEEEECCCCCCCCCC
TVRLAGSSGANPFACISAGITCLWGPAHGGANEACLNMLEEIGDVSRINEYITRAKDKND
EEEEECCCCCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFRLMGFGHRVYKNFDPRATLMRDTCHEVLDELGLHNDRLFKLALELERIALEDDFFITR
CEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
KLYPNVDFYSGIVQRALGIPTSMFTAIFATARTVGWVAQWNEMISDPEQKIGRPRQLYVG
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEE
VPRRDVP
CCCCCCC
>Mature Secondary Structure 
PPQDVATLSPGENKKTIELPILSGNEGPDVIDIRSLYAQSNMFTYDPGFVSTASCKSAI
CCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHCCCCEEECCCCCCHHHHHCEE
TFIDGDQGILLYRGYPIEQLATRCDFMEVSYLLLNGELPTADQKKQFVNNIKNHTMLHEQ
EEEECCCCEEEECCCCHHHHHHHCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
MIKFLSGFRRDAHPMAVMVGVVGALSAFYHDAMDVSDAPHREQSIFRLIAKLPTITAIAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEE
KYNIGQPFIYPRNDLGFTENFLHMMFATPAEKYVPNPVIVRALDRILILHADHEQNASTS
EEECCCCCEEECCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHEEEEECCCCCCCCCC
TVRLAGSSGANPFACISAGITCLWGPAHGGANEACLNMLEEIGDVSRINEYITRAKDKND
EEEEECCCCCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFRLMGFGHRVYKNFDPRATLMRDTCHEVLDELGLHNDRLFKLALELERIALEDDFFITR
CEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
KLYPNVDFYSGIVQRALGIPTSMFTAIFATARTVGWVAQWNEMISDPEQKIGRPRQLYVG
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEE
VPRRDVP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]