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The map label for this gene is ndhF [H]

Identifier: 114330809

GI number: 114330809

Start: 815296

End: 816990

Strand: Direct

Name: ndhF [H]

Synonym: Neut_0799

Alternate gene names: 114330809

Gene position: 815296-816990 (Clockwise)

Preceding gene: 114330808

Following gene: 114330810

Centisome position: 30.64

GC content: 57.23

Gene sequence:

>1695_bases
GTGAACGATTGGGTCGCGTTTTGTCTAGTGGCTTTGCCGGCCCTGCCGGTTCTTTCTGCCGTCGCCGCACCGTTGATGTC
TCGCTCCGCCAAGCATCTGGCTGCGCGGATCAGCACAGGTTTCATGGGATTGACTCTGCTGCTGGCGCTGGCCCTCTTGA
TTGCGGTACTGCAAATGCCGGCTGAACAGGTCCTGGTGTTGCGCGCCGGGCCGCTGACCTTAATCCTTGACCACTTAGCC
GTAGTCATGGCGATACTGGTCTCGGGCATTAGTTTGATCGTGCATATGTACGCGGTGCAGTATATGGCTGACGAGCCCAG
CTATGCGCGGTTCTTCGCGTTGCTGGACCTGATGACGGCGAACATTTTGCTGCTGGTGTGCGCATCCGACTTGCTCACGC
TGGTCATGTCCTGGTTCCTGGTGGGGGTGCTACTGTACTTCCTGCTGGGACACGACACCACGCGCGCGGCCAGCGGACGC
TATGCGTTCTGGACTTTCATCACCTATCGCGCCGGCGACCTGCCTTTGCTCGGGGCGGCTCTTGTGCTTGGACACGCTTA
CGGCACGATGCAGTTGTCCGAACTGTTCGCGCGCATCGCGGCCAATCCCGACGTGACAGTCGCCGGCTGGCCGGCGACAA
GCACTGCAGCGATGCTGGTGGCGTTGGCTGCGTTTGCGCGTTCCGCGCAGTTTCCGCTGCATTCCTGGTTGCCGTACACA
ATGGAGGGGCCAACCCCGGTGTCGGCACTGATGCATGCCGGTATCGTCAACGCCGGCGGCTTTTTGCTCAACCGTTTTGC
CCCCTTGTTCGTACACACGTCGGACGTGCTGCACGTGGTGTTCACGGTTGGATTGTTGACCGCGCTGATTGGATCGGCAC
TGATGCTCACGCAAAACGATATCAAGAAATCGCTGGGTTACTCAACGATGGGTCAGATGGGTTTCATGGTGATGGAGTGC
GGGCTCGGAGCATTTTCGCTCGCAGTGTACCATCTTGTTGCCCACGGTCTGTTCAAGGCTACGCTGTTTCTCGGGTCGGG
TGGTGTGATCAGCGATGCGCGCCGCAACGATGGCGTGCCTCCTGACGATATCTACACCTCGGTAGTCGAACGTAAGCCGC
TGCGCTTGGTGCGCCTGCCTTGGTTGGTCGCATTGGCAGTGACAGTGCTAGTTCCGGCCTTCGTGCTCGGCTTTTCGCAT
CTGCTGGTGGCAGCTGATTTCGTGCATAAGCAAGGGGCGGTCATTCTGCTGTTTTTCGGCTGGATCACGGGTGCCCAGGT
CTTTTTCTCGATTCACCGTTTGTCTACCGAGAATCCATGGCGGTTGATGGGAATGATCTTCCTGTCGTTTGTCATCGTCG
TTGTGGGTTATACGATAATAGGGCATCTGTTCGAGCAGTTCCTCTATCCCGACGCAACTCTGGTAAATGCGATATATGCC
GCAGCAGGAGTCGATCTGATTGTTTTCGATATCATCGTGGTGGTGCTGACCTTGATCTTGGTGGCAGCATGGCTCGTCAT
TTTGTACGCTGACTCCTCGAATATGGGTGGACGTTCGAGGGTCAGGAATATCGTTTACCTCAATCTTTACGCTTTGTTCT
CGCGCGAGTTCTACGTGGTTGATGTGTTTGCGCGTCTGGGGCGCATGCTACAAGACGGCTCCAAGCGCGTGAACGTATGG
CTGCGCTGGGTTTGA

Upstream 100 bases:

>100_bases
GGGTTCGCCTCGGTTCAGAAACGGGCGTCCAGTGCAATCCGGAAGAGCGGGTCCACATCTTGACGCATTTCTCTTTCCCT
TTATCGAGGACATATCTGTC

Downstream 100 bases:

>100_bases
TTCATGGAGACGACACCGATGATGATCTCACTGATGGCATTGTTGGCAGTTTTTTTTCTGCCGCTATTCCCGTTTAGCAT
GGTTTTCAACACCGTATTGC

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MNDWVAFCLVALPALPVLSAVAAPLMSRSAKHLAARISTGFMGLTLLLALALLIAVLQMPAEQVLVLRAGPLTLILDHLA
VVMAILVSGISLIVHMYAVQYMADEPSYARFFALLDLMTANILLLVCASDLLTLVMSWFLVGVLLYFLLGHDTTRAASGR
YAFWTFITYRAGDLPLLGAALVLGHAYGTMQLSELFARIAANPDVTVAGWPATSTAAMLVALAAFARSAQFPLHSWLPYT
MEGPTPVSALMHAGIVNAGGFLLNRFAPLFVHTSDVLHVVFTVGLLTALIGSALMLTQNDIKKSLGYSTMGQMGFMVMEC
GLGAFSLAVYHLVAHGLFKATLFLGSGGVISDARRNDGVPPDDIYTSVVERKPLRLVRLPWLVALAVTVLVPAFVLGFSH
LLVAADFVHKQGAVILLFFGWITGAQVFFSIHRLSTENPWRLMGMIFLSFVIVVVGYTIIGHLFEQFLYPDATLVNAIYA
AAGVDLIVFDIIVVVLTLILVAAWLVILYADSSNMGGRSRVRNIVYLNLYALFSREFYVVDVFARLGRMLQDGSKRVNVW
LRWV

Sequences:

>Translated_564_residues
MNDWVAFCLVALPALPVLSAVAAPLMSRSAKHLAARISTGFMGLTLLLALALLIAVLQMPAEQVLVLRAGPLTLILDHLA
VVMAILVSGISLIVHMYAVQYMADEPSYARFFALLDLMTANILLLVCASDLLTLVMSWFLVGVLLYFLLGHDTTRAASGR
YAFWTFITYRAGDLPLLGAALVLGHAYGTMQLSELFARIAANPDVTVAGWPATSTAAMLVALAAFARSAQFPLHSWLPYT
MEGPTPVSALMHAGIVNAGGFLLNRFAPLFVHTSDVLHVVFTVGLLTALIGSALMLTQNDIKKSLGYSTMGQMGFMVMEC
GLGAFSLAVYHLVAHGLFKATLFLGSGGVISDARRNDGVPPDDIYTSVVERKPLRLVRLPWLVALAVTVLVPAFVLGFSH
LLVAADFVHKQGAVILLFFGWITGAQVFFSIHRLSTENPWRLMGMIFLSFVIVVVGYTIIGHLFEQFLYPDATLVNAIYA
AAGVDLIVFDIIVVVLTLILVAAWLVILYADSSNMGGRSRVRNIVYLNLYALFSREFYVVDVFARLGRMLQDGSKRVNVW
LRWV
>Mature_564_residues
MNDWVAFCLVALPALPVLSAVAAPLMSRSAKHLAARISTGFMGLTLLLALALLIAVLQMPAEQVLVLRAGPLTLILDHLA
VVMAILVSGISLIVHMYAVQYMADEPSYARFFALLDLMTANILLLVCASDLLTLVMSWFLVGVLLYFLLGHDTTRAASGR
YAFWTFITYRAGDLPLLGAALVLGHAYGTMQLSELFARIAANPDVTVAGWPATSTAAMLVALAAFARSAQFPLHSWLPYT
MEGPTPVSALMHAGIVNAGGFLLNRFAPLFVHTSDVLHVVFTVGLLTALIGSALMLTQNDIKKSLGYSTMGQMGFMVMEC
GLGAFSLAVYHLVAHGLFKATLFLGSGGVISDARRNDGVPPDDIYTSVVERKPLRLVRLPWLVALAVTVLVPAFVLGFSH
LLVAADFVHKQGAVILLFFGWITGAQVFFSIHRLSTENPWRLMGMIFLSFVIVVVGYTIIGHLFEQFLYPDATLVNAIYA
AAGVDLIVFDIIVVVLTLILVAAWLVILYADSSNMGGRSRVRNIVYLNLYALFSREFYVVDVFARLGRMLQDGSKRVNVW
LRWV

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=355, Percent_Identity=30.1408450704225, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI1788614, Length=327, Percent_Identity=37.9204892966361, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788829, Length=351, Percent_Identity=27.6353276353276, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788831, Length=340, Percent_Identity=27.9411764705882, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788827, Length=377, Percent_Identity=27.3209549071618, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 61415; Mature: 61415

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDWVAFCLVALPALPVLSAVAAPLMSRSAKHLAARISTGFMGLTLLLALALLIAVLQMP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEQVLVLRAGPLTLILDHLAVVMAILVSGISLIVHMYAVQYMADEPSYARFFALLDLMTA
HHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NILLLVCASDLLTLVMSWFLVGVLLYFLLGHDTTRAASGRYAFWTFITYRAGDLPLLGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCHHHHHH
LVLGHAYGTMQLSELFARIAANPDVTVAGWPATSTAAMLVALAAFARSAQFPLHSWLPYT
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCC
MEGPTPVSALMHAGIVNAGGFLLNRFAPLFVHTSDVLHVVFTVGLLTALIGSALMLTQND
CCCCCHHHHHHHHCHHCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKKSLGYSTMGQMGFMVMECGLGAFSLAVYHLVAHGLFKATLFLGSGGVISDARRNDGVP
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PDDIYTSVVERKPLRLVRLPWLVALAVTVLVPAFVLGFSHLLVAADFVHKQGAVILLFFG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
WITGAQVFFSIHRLSTENPWRLMGMIFLSFVIVVVGYTIIGHLFEQFLYPDATLVNAIYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAGVDLIVFDIIVVVLTLILVAAWLVILYADSSNMGGRSRVRNIVYLNLYALFSREFYVV
HHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
DVFARLGRMLQDGSKRVNVWLRWV
HHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MNDWVAFCLVALPALPVLSAVAAPLMSRSAKHLAARISTGFMGLTLLLALALLIAVLQMP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEQVLVLRAGPLTLILDHLAVVMAILVSGISLIVHMYAVQYMADEPSYARFFALLDLMTA
HHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NILLLVCASDLLTLVMSWFLVGVLLYFLLGHDTTRAASGRYAFWTFITYRAGDLPLLGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCHHHHHH
LVLGHAYGTMQLSELFARIAANPDVTVAGWPATSTAAMLVALAAFARSAQFPLHSWLPYT
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCC
MEGPTPVSALMHAGIVNAGGFLLNRFAPLFVHTSDVLHVVFTVGLLTALIGSALMLTQND
CCCCCHHHHHHHHCHHCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKKSLGYSTMGQMGFMVMECGLGAFSLAVYHLVAHGLFKATLFLGSGGVISDARRNDGVP
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PDDIYTSVVERKPLRLVRLPWLVALAVTVLVPAFVLGFSHLLVAADFVHKQGAVILLFFG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
WITGAQVFFSIHRLSTENPWRLMGMIFLSFVIVVVGYTIIGHLFEQFLYPDATLVNAIYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAGVDLIVFDIIVVVLTLILVAAWLVILYADSSNMGGRSRVRNIVYLNLYALFSREFYVV
HHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
DVFARLGRMLQDGSKRVNVWLRWV
HHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]