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The map label for this gene is nth [H]

Identifier: 114330659

GI number: 114330659

Start: 655449

End: 656108

Strand: Reverse

Name: nth [H]

Synonym: Neut_0644

Alternate gene names: 114330659

Gene position: 656108-655449 (Counterclockwise)

Preceding gene: 114330660

Following gene: 114330653

Centisome position: 24.66

GC content: 51.21

Gene sequence:

>660_bases
ATGAATACCACCAAACGCCGGGAGATATTCACCCGATTCAGGCAGGCCAACCCCCATCCGACCACGGAACTCGAATACAG
CACACCGTTTCAGTTACTGGTTGCCGTCATCCTCTCGGCACAAGCTACTGATAAAAGCGTCAACCTGGCTACACGCAAAC
TGTTCCCGATGGCCGATACACCAGAGAAAATTCTGCGACTGGGAGAAATCGGCCTCAGCCCATTCATTCAGCGCATCGGT
CTATACCGTACCAAAACCAGAAATATCCTCGCTACCTGCCAGTTACTGATCGAACAACATCACAGCGAAGTCCCGCACAC
CCGCACAGAACTGGAGAAACTGCCGGGAGTCGGCCGGAAAACCGCCAATGTGATTCTGAATACCGCCTTTGGCGAACCCA
CCATCGCAGTCGATACCCACATCTTCCGCCTGGCTAACCGCACCGGCATCGCTCCGGGTAAAAATGTGCTGGAAGTTGAG
CAAAAATTGCTCAAGGTTATTCCGGAAGAATTTCGGCAAGATGCTCACCACTGGCTAATTCTGCACGGACGCTATACCTG
CAAAGCCAGGAAGCCCTTATGTCAGCAATGCCTGATTGTAGATTTATGCGAATTCAAAGAGAAGAACCTGGAAGAAACTA
CACCCTCACCCGATTTATAA

Upstream 100 bases:

>100_bases
CCGCTACACAACCAGCAACCGATATGAAAAAAGCAGCCATCCAAGCAGCGATGGAACGCGCACGCACAGCACTTGCGCGT
TCCAACATTCCGTTTAAAGA

Downstream 100 bases:

>100_bases
ATCGCACTACCTAACTAGAACGCGCCAGCGCACTCTGCAATTATGGCTCATGCATAGTGTAAAATTTTTCGACAGCACTT
GCTGGATTGCTTCACGCCTG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNTTKRREIFTRFRQANPHPTTELEYSTPFQLLVAVILSAQATDKSVNLATRKLFPMADTPEKILRLGEIGLSPFIQRIG
LYRTKTRNILATCQLLIEQHHSEVPHTRTELEKLPGVGRKTANVILNTAFGEPTIAVDTHIFRLANRTGIAPGKNVLEVE
QKLLKVIPEEFRQDAHHWLILHGRYTCKARKPLCQQCLIVDLCEFKEKNLEETTPSPDL

Sequences:

>Translated_219_residues
MNTTKRREIFTRFRQANPHPTTELEYSTPFQLLVAVILSAQATDKSVNLATRKLFPMADTPEKILRLGEIGLSPFIQRIG
LYRTKTRNILATCQLLIEQHHSEVPHTRTELEKLPGVGRKTANVILNTAFGEPTIAVDTHIFRLANRTGIAPGKNVLEVE
QKLLKVIPEEFRQDAHHWLILHGRYTCKARKPLCQQCLIVDLCEFKEKNLEETTPSPDL
>Mature_219_residues
MNTTKRREIFTRFRQANPHPTTELEYSTPFQLLVAVILSAQATDKSVNLATRKLFPMADTPEKILRLGEIGLSPFIQRIG
LYRTKTRNILATCQLLIEQHHSEVPHTRTELEKLPGVGRKTANVILNTAFGEPTIAVDTHIFRLANRTGIAPGKNVLEVE
QKLLKVIPEEFRQDAHHWLILHGRYTCKARKPLCQQCLIVDLCEFKEKNLEETTPSPDL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=67.3076923076923, Blast_Score=301, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=31.638418079096, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=125, Percent_Identity=35.2, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324530, Length=198, Percent_Identity=26.7676767676768, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550361, Length=188, Percent_Identity=32.9787234042553, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24968; Mature: 24968

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTKRREIFTRFRQANPHPTTELEYSTPFQLLVAVILSAQATDKSVNLATRKLFPMADT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
PEKILRLGEIGLSPFIQRIGLYRTKTRNILATCQLLIEQHHSEVPHTRTELEKLPGVGRK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
TANVILNTAFGEPTIAVDTHIFRLANRTGIAPGKNVLEVEQKLLKVIPEEFRQDAHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCEEEE
LHGRYTCKARKPLCQQCLIVDLCEFKEKNLEETTPSPDL
EECCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNTTKRREIFTRFRQANPHPTTELEYSTPFQLLVAVILSAQATDKSVNLATRKLFPMADT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
PEKILRLGEIGLSPFIQRIGLYRTKTRNILATCQLLIEQHHSEVPHTRTELEKLPGVGRK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
TANVILNTAFGEPTIAVDTHIFRLANRTGIAPGKNVLEVEQKLLKVIPEEFRQDAHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCEEEE
LHGRYTCKARKPLCQQCLIVDLCEFKEKNLEETTPSPDL
EECCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]