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The map label for this gene is mtkB [H]

Identifier: 114330620

GI number: 114330620

Start: 614210

End: 615097

Strand: Reverse

Name: mtkB [H]

Synonym: Neut_0605

Alternate gene names: 114330620

Gene position: 615097-614210 (Counterclockwise)

Preceding gene: 114330621

Following gene: 114330619

Centisome position: 23.11

GC content: 52.59

Gene sequence:

>888_bases
GTGGCAATTCTCATTAATGAGCGGACGCGGATCATCGTACAAGGTTTTACCGGCCGGATTGGCACCTTCCACGCTCAGGA
AATGATCGATTATGGATCCAATGTGGTAGGAGGCGTAACGCCCGGTAAAGGGGGACAGAAACACCTGGGACTGCCAGTAT
TCAATACCGTCAGGGAAGCGGTTGAACAGGCTGGTGCGGAAGCCAGTATCGTATTTGTCCCGCCTGCCTTTGCTGCAGAT
TCCATCATGGAAGCAGCTGATGCAGGAATCAAATATTGTGTCTCCATCACGGATGGTATCCCGACCCAGGACATGATGAC
CGTCAAGAATTTCCTGCGCCTCTTTCCAAAACAAAACCGGATGGTATTGTCTGGACCTAACTGTTCCGGCACCATCAGTC
CAGGGCGTTCCATGCTCGGTATTATGCCAGGGCATATTTACAGCCAGGGGATAGTCGGCGTGGTAGGCCGTTCCGGCACA
CTCGGCTATGAGGCCGCCGATCAGATGCGGCGACTGAATATCGGGATTTCCACCTCGGTTGGCATTGGTGGAGATCCGAT
CATCGGTAGCTCACACCGGGATATTCTGGAAAAACTGGAAGAAGATCCGGAAACCAAAATTGCATTGATGATTGGTGAAA
TCGGCGGCCCGATGGAAGTGGAAGCCGGACTATTCGCCAAAGAAAACATGCGCAAACCTCTGGTTGCCTATATTGCCGGA
TTAACTGCTCCTTCCGGAAGAAGAATGGGGCATGCCGGAGCAATCATCTCATCAACCGGCGAAAGTGCAGCAGAAAAAGT
GGAACAACTGCAAGAACTGGGTGTTACTATCTGCCCGACCCCGTCCATGATGGGGGAAACGGTCGCAAAAGTGCTGGCTG
GCCTCTGA

Upstream 100 bases:

>100_bases
TTATCACCGCAGAAACTCTGGCCGAAGCAGCCGAGAAAGCAGTACACGCACGCAACCAGGTCGTAGCCTGAATAACCGAA
ACCAGAAAGGGGAACAAAAA

Downstream 100 bases:

>100_bases
TTCAGCTTTGGATTCTCCTGTCCCGAGTAAAGAAAATCATCAATAATGATCTTGTTATTAAAATGCCGGAAGAGCATTAT
TCGCACTAAAATGGATGATT

Product: succinyl-CoA synthetase subunit alpha

Products: NA

Alternate protein names: MTK-alpha; Malate thiokinase; Malyl-CoA synthetase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAILINERTRIIVQGFTGRIGTFHAQEMIDYGSNVVGGVTPGKGGQKHLGLPVFNTVREAVEQAGAEASIVFVPPAFAAD
SIMEAADAGIKYCVSITDGIPTQDMMTVKNFLRLFPKQNRMVLSGPNCSGTISPGRSMLGIMPGHIYSQGIVGVVGRSGT
LGYEAADQMRRLNIGISTSVGIGGDPIIGSSHRDILEKLEEDPETKIALMIGEIGGPMEVEAGLFAKENMRKPLVAYIAG
LTAPSGRRMGHAGAIISSTGESAAEKVEQLQELGVTICPTPSMMGETVAKVLAGL

Sequences:

>Translated_295_residues
MAILINERTRIIVQGFTGRIGTFHAQEMIDYGSNVVGGVTPGKGGQKHLGLPVFNTVREAVEQAGAEASIVFVPPAFAAD
SIMEAADAGIKYCVSITDGIPTQDMMTVKNFLRLFPKQNRMVLSGPNCSGTISPGRSMLGIMPGHIYSQGIVGVVGRSGT
LGYEAADQMRRLNIGISTSVGIGGDPIIGSSHRDILEKLEEDPETKIALMIGEIGGPMEVEAGLFAKENMRKPLVAYIAG
LTAPSGRRMGHAGAIISSTGESAAEKVEQLQELGVTICPTPSMMGETVAKVLAGL
>Mature_294_residues
AILINERTRIIVQGFTGRIGTFHAQEMIDYGSNVVGGVTPGKGGQKHLGLPVFNTVREAVEQAGAEASIVFVPPAFAADS
IMEAADAGIKYCVSITDGIPTQDMMTVKNFLRLFPKQNRMVLSGPNCSGTISPGRSMLGIMPGHIYSQGIVGVVGRSGTL
GYEAADQMRRLNIGISTSVGIGGDPIIGSSHRDILEKLEEDPETKIALMIGEIGGPMEVEAGLFAKENMRKPLVAYIAGL
TAPSGRRMGHAGAIISSTGESAAEKVEQLQELGVTICPTPSMMGETVAKVLAGL

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the succinate/malate CoA ligase alpha subunit family [H]

Homologues:

Organism=Homo sapiens, GI109452591, Length=292, Percent_Identity=52.0547945205479, Blast_Score=255, Evalue=3e-68,
Organism=Homo sapiens, GI38569423, Length=321, Percent_Identity=28.9719626168224, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI38569421, Length=321, Percent_Identity=28.9719626168224, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786949, Length=292, Percent_Identity=55.8219178082192, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17553126, Length=296, Percent_Identity=50.3378378378378, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17550194, Length=296, Percent_Identity=50.3378378378378, Blast_Score=272, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17551266, Length=322, Percent_Identity=25.4658385093168, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17557344, Length=265, Percent_Identity=26.0377358490566, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324716, Length=299, Percent_Identity=42.1404682274247, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI21356559, Length=288, Percent_Identity=48.6111111111111, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI28574296, Length=295, Percent_Identity=46.7796610169492, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI281363477, Length=321, Percent_Identity=27.1028037383178, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281363475, Length=321, Percent_Identity=27.1028037383178, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281363473, Length=321, Percent_Identity=27.1028037383178, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017440
- InterPro:   IPR003781
- InterPro:   IPR005810
- InterPro:   IPR005811
- InterPro:   IPR016040
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: =6.2.1.9 [H]

Molecular weight: Translated: 30940; Mature: 30809

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00399 SUCCINYL_COA_LIG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILINERTRIIVQGFTGRIGTFHAQEMIDYGSNVVGGVTPGKGGQKHLGLPVFNTVREA
CEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
VEQAGAEASIVFVPPAFAADSIMEAADAGIKYCVSITDGIPTQDMMTVKNFLRLFPKQNR
HHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCC
MVLSGPNCSGTISPGRSMLGIMPGHIYSQGIVGVVGRSGTLGYEAADQMRRLNIGISTSV
EEEECCCCCCCCCCCCHHHHCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHHCCCEEECC
GIGGDPIIGSSHRDILEKLEEDPETKIALMIGEIGGPMEVEAGLFAKENMRKPLVAYIAG
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCHHHCCCHHHHHHHH
LTAPSGRRMGHAGAIISSTGESAAEKVEQLQELGVTICPTPSMMGETVAKVLAGL
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AILINERTRIIVQGFTGRIGTFHAQEMIDYGSNVVGGVTPGKGGQKHLGLPVFNTVREA
EEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
VEQAGAEASIVFVPPAFAADSIMEAADAGIKYCVSITDGIPTQDMMTVKNFLRLFPKQNR
HHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCC
MVLSGPNCSGTISPGRSMLGIMPGHIYSQGIVGVVGRSGTLGYEAADQMRRLNIGISTSV
EEEECCCCCCCCCCCCHHHHCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHHCCCEEECC
GIGGDPIIGSSHRDILEKLEEDPETKIALMIGEIGGPMEVEAGLFAKENMRKPLVAYIAG
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCHHHCCCHHHHHHHH
LTAPSGRRMGHAGAIISSTGESAAEKVEQLQELGVTICPTPSMMGETVAKVLAGL
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961516 [H]