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The map label for this gene is rluB [H]

Identifier: 114330607

GI number: 114330607

Start: 599523

End: 600341

Strand: Direct

Name: rluB [H]

Synonym: Neut_0592

Alternate gene names: 114330607

Gene position: 599523-600341 (Clockwise)

Preceding gene: 114330606

Following gene: 114330609

Centisome position: 22.53

GC content: 51.4

Gene sequence:

>819_bases
ATGTCGGTTAATCGGCTAAAGAATCAAAAGCCCAGGCGCACGCACAGATCACCTCCTGCCTCGAAGAACCTCAGCGCTGC
ACCTCCTGCCGAAACTGACAGGTTACAGAAGTTGCTGGCACGCAATGGCTTGGGTTCCCGACGGGAAATTGAAGCCATGA
TTGCAGCCGGTCGTGTGAGTATCAATAATACGACGGCAAAACTCGGCGATCGAGCTGGGCTCGGGGATAAGATACGGATT
GATGGAAGAATTATCCGACTTAGCATGCCTGCACTTTTGCCACGGATTATCATTTACCACAAGCCGGAGGGGGAAATTGT
CAGTGCGCGTGATCCACAGGGGCGGCTATCGGTATTCGATAAATTGCCGCACATTCAGTCTTCCAAATGGATAGCAATAG
GCAGGCTGGATTTTAATACCAGCGGATTGCTTATTTTTACAACCGACGGCGCCTTGGCCAATCGCCTGATGCATCCGCGT
TACCAGATGGAACGGGAGTACGCAGTTCGCATTGTTGGGGAGCTGTCCCCGGAACAAATTACTCATTTGACTACTGGTAT
TGAACTGGAAGATGGTTCAGCAAAATTTGATCAGTTGCTTGATGAAGGTGGGCTGGGAACGAATCACTGGTATCGCATCG
TACTGAAAGAAGGTAGAAACCGTGAGGTTCGCCGAATGTTCGAAGCGCTGGGGATAACAGTCAGCCGCCTGATGCGTGTC
AGGTTTGGCCCCGTTAATCTCCCGCCCAGACTTAAGCGCGGTATGTGGCTGGAGCTGGTCGATACAGAAGTCGAACGATT
ACTGGCGCTGATCAGTTAG

Upstream 100 bases:

>100_bases
TAACCAGTGGCCTGGGATTGCCGGCAGGAATGAAATTTCCTTTCTAAATTGTTTGGCTGCGTGTTTTTGACAGACCGACT
ACAACACAGGACTTATATTC

Downstream 100 bases:

>100_bases
CTCGCTTCCAGACAAATTCCTTTCTACGCTTACAAGTCGCATCGTCTCACCATTAGAAGAAACTGATCTCCTGCACTGGC
TTCCAACCAGGTACAGGACA

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSVNRLKNQKPRRTHRSPPASKNLSAAPPAETDRLQKLLARNGLGSRREIEAMIAAGRVSINNTTAKLGDRAGLGDKIRI
DGRIIRLSMPALLPRIIIYHKPEGEIVSARDPQGRLSVFDKLPHIQSSKWIAIGRLDFNTSGLLIFTTDGALANRLMHPR
YQMEREYAVRIVGELSPEQITHLTTGIELEDGSAKFDQLLDEGGLGTNHWYRIVLKEGRNREVRRMFEALGITVSRLMRV
RFGPVNLPPRLKRGMWLELVDTEVERLLALIS

Sequences:

>Translated_272_residues
MSVNRLKNQKPRRTHRSPPASKNLSAAPPAETDRLQKLLARNGLGSRREIEAMIAAGRVSINNTTAKLGDRAGLGDKIRI
DGRIIRLSMPALLPRIIIYHKPEGEIVSARDPQGRLSVFDKLPHIQSSKWIAIGRLDFNTSGLLIFTTDGALANRLMHPR
YQMEREYAVRIVGELSPEQITHLTTGIELEDGSAKFDQLLDEGGLGTNHWYRIVLKEGRNREVRRMFEALGITVSRLMRV
RFGPVNLPPRLKRGMWLELVDTEVERLLALIS
>Mature_271_residues
SVNRLKNQKPRRTHRSPPASKNLSAAPPAETDRLQKLLARNGLGSRREIEAMIAAGRVSINNTTAKLGDRAGLGDKIRID
GRIIRLSMPALLPRIIIYHKPEGEIVSARDPQGRLSVFDKLPHIQSSKWIAIGRLDFNTSGLLIFTTDGALANRLMHPRY
QMEREYAVRIVGELSPEQITHLTTGIELEDGSAKFDQLLDEGGLGTNHWYRIVLKEGRNREVRRMFEALGITVSRLMRVR
FGPVNLPPRLKRGMWLELVDTEVERLLALIS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=53.0864197530864, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=31.6872427983539, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788510, Length=211, Percent_Identity=27.9620853080569, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 30661; Mature: 30530

Theoretical pI: Translated: 11.12; Mature: 11.12

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNRLKNQKPRRTHRSPPASKNLSAAPPAETDRLQKLLARNGLGSRREIEAMIAAGRVS
CCCCHHCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCEEE
INNTTAKLGDRAGLGDKIRIDGRIIRLSMPALLPRIIIYHKPEGEIVSARDPQGRLSVFD
ECCCHHHCCCCCCCCCEEEECCEEEEEECHHHHCEEEEEECCCCCEEECCCCCCHHHHHH
KLPHIQSSKWIAIGRLDFNTSGLLIFTTDGALANRLMHPRYQMEREYAVRIVGELSPEQI
HCCCCCCCCEEEEEEEEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHH
THLTTGIELEDGSAKFDQLLDEGGLGTNHWYRIVLKEGRNREVRRMFEALGITVSRLMRV
HHHHCCEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RFGPVNLPPRLKRGMWLELVDTEVERLLALIS
HCCCCCCCHHHHCCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVNRLKNQKPRRTHRSPPASKNLSAAPPAETDRLQKLLARNGLGSRREIEAMIAAGRVS
CCCHHCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCEEE
INNTTAKLGDRAGLGDKIRIDGRIIRLSMPALLPRIIIYHKPEGEIVSARDPQGRLSVFD
ECCCHHHCCCCCCCCCEEEECCEEEEEECHHHHCEEEEEECCCCCEEECCCCCCHHHHHH
KLPHIQSSKWIAIGRLDFNTSGLLIFTTDGALANRLMHPRYQMEREYAVRIVGELSPEQI
HCCCCCCCCEEEEEEEEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHH
THLTTGIELEDGSAKFDQLLDEGGLGTNHWYRIVLKEGRNREVRRMFEALGITVSRLMRV
HHHHCCEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RFGPVNLPPRLKRGMWLELVDTEVERLLALIS
HCCCCCCCHHHHCCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]