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The map label for this gene is ampD [H]

Identifier: 114330553

GI number: 114330553

Start: 557692

End: 558237

Strand: Reverse

Name: ampD [H]

Synonym: Neut_0535

Alternate gene names: 114330553

Gene position: 558237-557692 (Counterclockwise)

Preceding gene: 114330555

Following gene: 114330551

Centisome position: 20.98

GC content: 52.38

Gene sequence:

>546_bases
ATGCTAATCGATACGGACGGTTACCTCGTTCACGCCAGATACATTCCTTCTCCCAATTGCGATGAACGGCCGGACGGGAA
TGATATCAGCCTGCTGGTCATCCACGCCATCAGCCTGCCCCCGGAAGAATTTGGCGGTGAAGGCGTCATTGAACTGTTTA
CCAACCAGATCAACCCTCAAGCACACCCGTATTATCAAGAGCTGACAGGATTAAAAGTCTCCAGCCATTTTTTTATTCGC
CGTGATGGGGAAATCATCCAGTTTGTCCCCTGCACGCTGCGTGCCTGGCATGCCGGCGCCTCCTGCTGGCAAGGCCGCAC
CCGGTGCAACGATTTTTCCATTGGTATTGAGCTGGAAGGCAGTGATAATCAACCATTTGCACCAATTCAGTACGCCCGTT
TGGTTAAACTCACCCAAGCACTACAGGCAACCTACCCTATTCATGACATTGTCGGGCACGTCGATATTGCGCCACAAAGG
AAAACTGACCCAGGCCCCTGCTTTGACTGGCAAAGCTACCGGGAAGCACTCGAAATAACCCGGTAG

Upstream 100 bases:

>100_bases
CCGCGTTGCAAGATAATCACTCAATTAAAATGGTTAACGGGCGTTTTTATGGCGGCACCCTTAAAATTTCAGAACTCATG
CCGGCAATTCAATCATATCC

Downstream 100 bases:

>100_bases
TTTGCAGGTGATGCACTATGCTTGCCCGCCCTGCATCACCACCAGATTCAAACAGAGCCCGGCAATCAGCCAACCAGTTC
CATGGAATTAACTGGCCTGG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MLIDTDGYLVHARYIPSPNCDERPDGNDISLLVIHAISLPPEEFGGEGVIELFTNQINPQAHPYYQELTGLKVSSHFFIR
RDGEIIQFVPCTLRAWHAGASCWQGRTRCNDFSIGIELEGSDNQPFAPIQYARLVKLTQALQATYPIHDIVGHVDIAPQR
KTDPGPCFDWQSYREALEITR

Sequences:

>Translated_181_residues
MLIDTDGYLVHARYIPSPNCDERPDGNDISLLVIHAISLPPEEFGGEGVIELFTNQINPQAHPYYQELTGLKVSSHFFIR
RDGEIIQFVPCTLRAWHAGASCWQGRTRCNDFSIGIELEGSDNQPFAPIQYARLVKLTQALQATYPIHDIVGHVDIAPQR
KTDPGPCFDWQSYREALEITR
>Mature_181_residues
MLIDTDGYLVHARYIPSPNCDERPDGNDISLLVIHAISLPPEEFGGEGVIELFTNQINPQAHPYYQELTGLKVSSHFFIR
RDGEIIQFVPCTLRAWHAGASCWQGRTRCNDFSIGIELEGSDNQPFAPIQYARLVKLTQALQATYPIHDIVGHVDIAPQR
KTDPGPCFDWQSYREALEITR

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=176, Percent_Identity=56.8181818181818, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1787092, Length=122, Percent_Identity=46.7213114754098, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20450; Mature: 20450

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDTDGYLVHARYIPSPNCDERPDGNDISLLVIHAISLPPEEFGGEGVIELFTNQINPQ
CEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCCCEEEHHHHCCCCC
AHPYYQELTGLKVSSHFFIRRDGEIIQFVPCTLRAWHAGASCWQGRTRCNDFSIGIELEG
CCCHHHHHCCEEECCEEEEEECCCEEEEECCHHHHHHCCCHHHCCCCCCCCEEEEEEEEC
SDNQPFAPIQYARLVKLTQALQATYPIHDIVGHVDIAPQRKTDPGPCFDWQSYREALEIT
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
R
C
>Mature Secondary Structure
MLIDTDGYLVHARYIPSPNCDERPDGNDISLLVIHAISLPPEEFGGEGVIELFTNQINPQ
CEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCCCEEEHHHHCCCCC
AHPYYQELTGLKVSSHFFIRRDGEIIQFVPCTLRAWHAGASCWQGRTRCNDFSIGIELEG
CCCHHHHHCCEEECCEEEEEECCCEEEEECCHHHHHHCCCHHHCCCCCCCCEEEEEEEEC
SDNQPFAPIQYARLVKLTQALQATYPIHDIVGHVDIAPQRKTDPGPCFDWQSYREALEIT
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]