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The map label for this gene is phbI [H]

Identifier: 114330547

GI number: 114330547

Start: 552246

End: 553973

Strand: Reverse

Name: phbI [H]

Synonym: Neut_0529

Alternate gene names: 114330547

Gene position: 553973-552246 (Counterclockwise)

Preceding gene: 114330548

Following gene: 114330546

Centisome position: 20.82

GC content: 50.35

Gene sequence:

>1728_bases
ATGAGTTTCATCCTCCATGGTGTCGGTGTGTCCGACGGAATTGCGATCGGACGTGCTCATCTGGCCTCGTCAGCAGCGCT
GAACATCCTTCATTACCAGCTGCCAAAGGATCAAATTGATGCTGAATTGGCACGGTTACACAACGCATTTTCCACCGTGC
GGCTGGAGCTGGAAACACTAAAGAGTTCTACTGTACAAGGAGCTGGACCGGCTGAATTCAATGCATTTCTGGAACTGCAC
CAGATGATTCTGGATGATCCGATGTTTTCCCAGGCTGCACTGGACATTGTAAAGCAAGCGCAATGCAACGCCGAATGGGC
AATTACACAACAAATGGAAGTCCTGGTTGCACGCTTCGAGGAAATAGAAGATGCCTATTTACGTGAACGCAAAACCGATG
TTGCCCAGATAGCTGAACGGGTTCTGAAGGTGTTGCTCGGACATCCGGGGTATACGCCCCCGCCGGTGAAACAAGATGGC
TCCGGTATTCTGGTAGCACATGATTTGAGTCCGGCTGATGTCATGCAATACAAGCAGTATCAATTTTCTGCCTTTCTGAC
AGACATGGGCGGGCCAACCTCGCATACCGCTATTGTTGCACGCAGTCTCAATATTCCATCGATCGTGGCTATGCAGCACG
CCCAGCAGCTTATCCGCGAGAATGACAATCTGATTGTGGATGGCAATCAGGGCATCGTCATTATCAACCCGGATAAATAT
ATCCTCGCCGAATATCGTCTGAAGCAAAACCAGCTTGAGCTGGAGAAACGCAAACTCTGGCGTCTCCGCTTTGTAGCAGC
AGTGACACGGGATGGTACTCCGGTGGATTTGCTTGCCAATATCGAACTGCCGCAAGAAGTCGAGCGGGCAAAAGAACATG
GCGCAACCGGAATCGGTCTGTTTCGCAGTGAATTTCTGTTTTTGAACCGGGATGATCTGCCGGATGAAGAAGAACAGTTT
GAAGCCTATTCCACGGTGATCAAAGGCATGAATGGATCACCTGTCATCATTCGTACGTTTGATCTGGGTGCGGACAAGAA
TCTCAGAAGCGCCTATCGCATGGCTCCCAATCCGGCGCTTGGCCTGCGTGCGATCCGGATGAGCCTGGCCGAACCAGGCA
TGTTTCTGGCGCAACTGCGCGCCATTTTGCGCGCTTCAAGCCTGGGCCATACCCAGATCCTGATTCCGATGCTTTCCAGC
AGCCGGGAAGTTGATCAGACACTGCAAATGATCGAATTTGCCAAGCAAAGTTTACGAGATGAAAACAAGCCTTTCAATGA
AACTATTAAAATCGGCTGCATGGTGGAAATCCCGGCAACTGCGCTGAGCCTTGAGCTATTCATGCGCAAACTGGATTTTT
TATCCATCGGCACCAATGATCTCATTCAGTACACACTGGCAATTGACCGGACCGACGAAACCGTTGCGCATCTGTTTGAT
CCGCTGCATCCGGCCATTTTGCGATTATTGGCTCGGATCATCCAGAGTGCGACAAAAGCCGGCATACCGGTTTCTATCTG
CGGAGAAATGGCAGGCGATGCAAAATATACCCGTCTGTTACTCGGGATGGGGCTACGGCAGTTTTCCATGTATCCCACAC
AGCTACTGACTGTGAAACGGGAGGTACTGGATAGTTCTTTGCCCGATATTTCGCGTCTGATACAAAAGATACTCAAGGCG
GACGAGCCGGAGAAAATTCATGATCTGCTGCAGAAACTTAATAGCTGA

Upstream 100 bases:

>100_bases
GCAAGGGTACAGTGGTCGAGCTGGAAACGATCGGACCCGATGCAATTCAGGCCATGGAAGCATTAAAAGCGTTGATCAAT
GATTACTTCGGGGAAGGTGA

Downstream 100 bases:

>100_bases
TACATCCACCTCCCTAAGGAATACTGAATTTTCAGTAATACAATTAGATACAATGAGCATTCATTTTTCACATCTACAGT
CAGTTTTATTATTTTCAGAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSFILHGVGVSDGIAIGRAHLASSAALNILHYQLPKDQIDAELARLHNAFSTVRLELETLKSSTVQGAGPAEFNAFLELH
QMILDDPMFSQAALDIVKQAQCNAEWAITQQMEVLVARFEEIEDAYLRERKTDVAQIAERVLKVLLGHPGYTPPPVKQDG
SGILVAHDLSPADVMQYKQYQFSAFLTDMGGPTSHTAIVARSLNIPSIVAMQHAQQLIRENDNLIVDGNQGIVIINPDKY
ILAEYRLKQNQLELEKRKLWRLRFVAAVTRDGTPVDLLANIELPQEVERAKEHGATGIGLFRSEFLFLNRDDLPDEEEQF
EAYSTVIKGMNGSPVIIRTFDLGADKNLRSAYRMAPNPALGLRAIRMSLAEPGMFLAQLRAILRASSLGHTQILIPMLSS
SREVDQTLQMIEFAKQSLRDENKPFNETIKIGCMVEIPATALSLELFMRKLDFLSIGTNDLIQYTLAIDRTDETVAHLFD
PLHPAILRLLARIIQSATKAGIPVSICGEMAGDAKYTRLLLGMGLRQFSMYPTQLLTVKREVLDSSLPDISRLIQKILKA
DEPEKIHDLLQKLNS

Sequences:

>Translated_575_residues
MSFILHGVGVSDGIAIGRAHLASSAALNILHYQLPKDQIDAELARLHNAFSTVRLELETLKSSTVQGAGPAEFNAFLELH
QMILDDPMFSQAALDIVKQAQCNAEWAITQQMEVLVARFEEIEDAYLRERKTDVAQIAERVLKVLLGHPGYTPPPVKQDG
SGILVAHDLSPADVMQYKQYQFSAFLTDMGGPTSHTAIVARSLNIPSIVAMQHAQQLIRENDNLIVDGNQGIVIINPDKY
ILAEYRLKQNQLELEKRKLWRLRFVAAVTRDGTPVDLLANIELPQEVERAKEHGATGIGLFRSEFLFLNRDDLPDEEEQF
EAYSTVIKGMNGSPVIIRTFDLGADKNLRSAYRMAPNPALGLRAIRMSLAEPGMFLAQLRAILRASSLGHTQILIPMLSS
SREVDQTLQMIEFAKQSLRDENKPFNETIKIGCMVEIPATALSLELFMRKLDFLSIGTNDLIQYTLAIDRTDETVAHLFD
PLHPAILRLLARIIQSATKAGIPVSICGEMAGDAKYTRLLLGMGLRQFSMYPTQLLTVKREVLDSSLPDISRLIQKILKA
DEPEKIHDLLQKLNS
>Mature_574_residues
SFILHGVGVSDGIAIGRAHLASSAALNILHYQLPKDQIDAELARLHNAFSTVRLELETLKSSTVQGAGPAEFNAFLELHQ
MILDDPMFSQAALDIVKQAQCNAEWAITQQMEVLVARFEEIEDAYLRERKTDVAQIAERVLKVLLGHPGYTPPPVKQDGS
GILVAHDLSPADVMQYKQYQFSAFLTDMGGPTSHTAIVARSLNIPSIVAMQHAQQLIRENDNLIVDGNQGIVIINPDKYI
LAEYRLKQNQLELEKRKLWRLRFVAAVTRDGTPVDLLANIELPQEVERAKEHGATGIGLFRSEFLFLNRDDLPDEEEQFE
AYSTVIKGMNGSPVIIRTFDLGADKNLRSAYRMAPNPALGLRAIRMSLAEPGMFLAQLRAILRASSLGHTQILIPMLSSS
REVDQTLQMIEFAKQSLRDENKPFNETIKIGCMVEIPATALSLELFMRKLDFLSIGTNDLIQYTLAIDRTDETVAHLFDP
LHPAILRLLARIIQSATKAGIPVSICGEMAGDAKYTRLLLGMGLRQFSMYPTQLLTVKREVLDSSLPDISRLIQKILKAD
EPEKIHDLLQKLNS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=38.2198952879581, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI48994992, Length=517, Percent_Identity=32.8820116054159, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788726, Length=553, Percent_Identity=32.368896925859, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1789193, Length=521, Percent_Identity=33.3973128598848, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1787994, Length=406, Percent_Identity=28.8177339901478, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI226510935, Length=190, Percent_Identity=27.8947368421053, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64089; Mature: 63958

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFILHGVGVSDGIAIGRAHLASSAALNILHYQLPKDQIDAELARLHNAFSTVRLELETL
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KSSTVQGAGPAEFNAFLELHQMILDDPMFSQAALDIVKQAQCNAEWAITQQMEVLVARFE
HHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EIEDAYLRERKTDVAQIAERVLKVLLGHPGYTPPPVKQDGSGILVAHDLSPADVMQYKQY
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
QFSAFLTDMGGPTSHTAIVARSLNIPSIVAMQHAQQLIRENDNLIVDGNQGIVIINPDKY
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCE
ILAEYRLKQNQLELEKRKLWRLRFVAAVTRDGTPVDLLANIELPQEVERAKEHGATGIGL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEH
FRSEFLFLNRDDLPDEEEQFEAYSTVIKGMNGSPVIIRTFDLGADKNLRSAYRMAPNPAL
HHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHH
GLRAIRMSLAEPGMFLAQLRAILRASSLGHTQILIPMLSSSREVDQTLQMIEFAKQSLRD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHCC
ENKPFNETIKIGCMVEIPATALSLELFMRKLDFLSIGTNDLIQYTLAIDRTDETVAHLFD
CCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCHHHHHHHHC
PLHPAILRLLARIIQSATKAGIPVSICGEMAGDAKYTRLLLGMGLRQFSMYPTQLLTVKR
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHCCHHHHHHHHH
EVLDSSLPDISRLIQKILKADEPEKIHDLLQKLNS
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SFILHGVGVSDGIAIGRAHLASSAALNILHYQLPKDQIDAELARLHNAFSTVRLELETL
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KSSTVQGAGPAEFNAFLELHQMILDDPMFSQAALDIVKQAQCNAEWAITQQMEVLVARFE
HHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EIEDAYLRERKTDVAQIAERVLKVLLGHPGYTPPPVKQDGSGILVAHDLSPADVMQYKQY
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
QFSAFLTDMGGPTSHTAIVARSLNIPSIVAMQHAQQLIRENDNLIVDGNQGIVIINPDKY
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCE
ILAEYRLKQNQLELEKRKLWRLRFVAAVTRDGTPVDLLANIELPQEVERAKEHGATGIGL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEH
FRSEFLFLNRDDLPDEEEQFEAYSTVIKGMNGSPVIIRTFDLGADKNLRSAYRMAPNPAL
HHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHH
GLRAIRMSLAEPGMFLAQLRAILRASSLGHTQILIPMLSSSREVDQTLQMIEFAKQSLRD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHCC
ENKPFNETIKIGCMVEIPATALSLELFMRKLDFLSIGTNDLIQYTLAIDRTDETVAHLFD
CCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCHHHHHHHHC
PLHPAILRLLARIIQSATKAGIPVSICGEMAGDAKYTRLLLGMGLRQFSMYPTQLLTVKR
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHCCHHHHHHHHH
EVLDSSLPDISRLIQKILKADEPEKIHDLLQKLNS
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]