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The map label for this gene is mycA [H]

Identifier: 114330478

GI number: 114330478

Start: 471867

End: 473693

Strand: Direct

Name: mycA [H]

Synonym: Neut_0458

Alternate gene names: 114330478

Gene position: 471867-473693 (Clockwise)

Preceding gene: 114330476

Following gene: 114330479

Centisome position: 17.73

GC content: 46.14

Gene sequence:

>1827_bases
TTGAGTTCGATAATAGCCAAATTGGATGTTCTGCCGGCGCAAGAAGGTAATTCCCTATCTTCCGAAGCAGGTGTTGTTTC
GGCAACGCCCACAATTAATTTGTTGAGCCAGAGAAGAGCTGATTTTACAACATTGGTGGAGTCGCTTGAGTACGCAGCAC
AAGGAGAAACTGGACATAACTTCTATGACGCAAAAGGTAATCTCAAGTCTGTACTTACATATAAAAATTTATGCGCTAAT
GCAAAAATAATTGCAAGACGGCTGTCTGGCCTTGGGCTGGCACGCAACAGCCGCGTTGCTCTGATCGCTGATACTACTCC
GGAGTTTGTTGAGCTGTTTTTTGCCTGCCGGTTTGCGGGCCTTGTTCCATTTGCCATGCCGGTGCCCGTTAATCTTGGTA
GCCGTGCGATTTATGTACAGCAGTTAAAAGGGATGCTTGGGGGCAGTGGGGCAAGCGTTGCAATAGCTAATCTTGATTTT
ATTGAGTTTCTCAATGAGGTAGTTGAGAGTCTGGGAAGCAGTGAACTCAAATGGAGTGGCACACCTGAGCAATTGAATCT
ACTACCTGAAGCTGATGTGGCGCTTTCTCCAAATACCCCGGATGAAACGGCTTATCTGCAATTTACTTCCGGCAGTACTC
GTTTGCCGAGAGGAGTAGTTATTACTGAGCGGGCTTTAATGACTAATCTGCGTGGTATCGTTTGCAATGGTCTTGATGTG
AGACCCGGTGATCGATGCGCTTCGTGGCTGCCTTTTTACCATGATATGGGTTTGGTTGGCTTGGTGTTGGCTCCTTTGGC
TGCCCAGTTATCCGTTGATTATTTGGCAACCCGGGATTTCGCTGTTCGTCCTTTGCAATGGCTTAAATTGATCAGTCGTA
ATCGCTGTACTATTGCCTTTAGTCAGCCTTTTGGTCTCAAGTTATGCACTTTGCGCGCACGCGATTCTGATTTAATGGAT
CTGGACTTAAGTTGCTGGCGTGCCGCTGGTGTTGGGGCTGAGATGATCCGGCTCGATACATTGAAAAGTTTTTCAGCCAA
GTTTTCAGCAGCGGGATTTAATGAAGATGCTTTCTTGCCTTGTTATGGTCTGGCTGAATCTACTTTGGCTGTGTCTTTCC
CTGATATCAATCAGGGGGTGAAAAGTTTACGTGTAGATGCCAAGACTTTGATCGAAAAAAAGATAGCAGTAAGAGTTCAG
GCAGATGGCAGGAAATTTAATGAGTTTGTGAATTGCGGACGTCCGTTGCCAGGCCATGAAATCAGAGTTGTCGATGACAC
TGATCAGGAAGTACCCCATTTGATGGTGGGTAGTATTCTAGTTAAAGGGGAAAGTGTGATGAATGGCTACTTTAACAACG
CAGAAGAGACTGCCAGAGCGATCAGATCAGATGGATGGTTAGATACTGGAGATATTGGTTTTCTGTTTGAAGGTGATTTA
TATATCACAGGGAGACGAACAGATGTCATTATTGTTAATGGTAGAAATATTCGAGCGCAGGATGTGGAAGAGCTGGCTGA
ACAGCAGCCAGAAGTCAGAACCCGTGAAGCTTCTGCTTTTGGAATCAGCGATGAAAATGGCTCGACGTCAGTCGTTTTGG
TTGTGGAATGCAGGCTGGCTTCAGCAGTAGATCGACAAGCACTCATTAACAGGCTTCAGCAGATGGTATATATGGCATTT
GGGGTAAGTTGTTTGGTTGAGCTGGTGCCCCCACATACTTTGCCACGGACATCTTCAGGAAAATTATCGCGCTTTGCGGC
ACGACAGGGGTTTTTGCGGCGTACAAATATGGAACAACCTGCTGCTACCAGTTATACAGTCGGCTAA

Upstream 100 bases:

>100_bases
AGATAGGCTTTTGATTATCTTGGCTAAATTAATTAATTCTTTAGTGTGCGCATTCGATCTATTCAGCAAATTGGCCATAA
AATATTTCTAGGGGAAGATT

Downstream 100 bases:

>100_bases
TAGAATAGCTAATCCTTCATCAGTAAAATCTGCTTATTTCTTTAGGGGAAGGAGTCAAAAGATTTCTTCTCGCATCCGGT
AGTACTTTATGGTTAGAACT

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSSIIAKLDVLPAQEGNSLSSEAGVVSATPTINLLSQRRADFTTLVESLEYAAQGETGHNFYDAKGNLKSVLTYKNLCAN
AKIIARRLSGLGLARNSRVALIADTTPEFVELFFACRFAGLVPFAMPVPVNLGSRAIYVQQLKGMLGGSGASVAIANLDF
IEFLNEVVESLGSSELKWSGTPEQLNLLPEADVALSPNTPDETAYLQFTSGSTRLPRGVVITERALMTNLRGIVCNGLDV
RPGDRCASWLPFYHDMGLVGLVLAPLAAQLSVDYLATRDFAVRPLQWLKLISRNRCTIAFSQPFGLKLCTLRARDSDLMD
LDLSCWRAAGVGAEMIRLDTLKSFSAKFSAAGFNEDAFLPCYGLAESTLAVSFPDINQGVKSLRVDAKTLIEKKIAVRVQ
ADGRKFNEFVNCGRPLPGHEIRVVDDTDQEVPHLMVGSILVKGESVMNGYFNNAEETARAIRSDGWLDTGDIGFLFEGDL
YITGRRTDVIIVNGRNIRAQDVEELAEQQPEVRTREASAFGISDENGSTSVVLVVECRLASAVDRQALINRLQQMVYMAF
GVSCLVELVPPHTLPRTSSGKLSRFAARQGFLRRTNMEQPAATSYTVG

Sequences:

>Translated_608_residues
MSSIIAKLDVLPAQEGNSLSSEAGVVSATPTINLLSQRRADFTTLVESLEYAAQGETGHNFYDAKGNLKSVLTYKNLCAN
AKIIARRLSGLGLARNSRVALIADTTPEFVELFFACRFAGLVPFAMPVPVNLGSRAIYVQQLKGMLGGSGASVAIANLDF
IEFLNEVVESLGSSELKWSGTPEQLNLLPEADVALSPNTPDETAYLQFTSGSTRLPRGVVITERALMTNLRGIVCNGLDV
RPGDRCASWLPFYHDMGLVGLVLAPLAAQLSVDYLATRDFAVRPLQWLKLISRNRCTIAFSQPFGLKLCTLRARDSDLMD
LDLSCWRAAGVGAEMIRLDTLKSFSAKFSAAGFNEDAFLPCYGLAESTLAVSFPDINQGVKSLRVDAKTLIEKKIAVRVQ
ADGRKFNEFVNCGRPLPGHEIRVVDDTDQEVPHLMVGSILVKGESVMNGYFNNAEETARAIRSDGWLDTGDIGFLFEGDL
YITGRRTDVIIVNGRNIRAQDVEELAEQQPEVRTREASAFGISDENGSTSVVLVVECRLASAVDRQALINRLQQMVYMAF
GVSCLVELVPPHTLPRTSSGKLSRFAARQGFLRRTNMEQPAATSYTVG
>Mature_607_residues
SSIIAKLDVLPAQEGNSLSSEAGVVSATPTINLLSQRRADFTTLVESLEYAAQGETGHNFYDAKGNLKSVLTYKNLCANA
KIIARRLSGLGLARNSRVALIADTTPEFVELFFACRFAGLVPFAMPVPVNLGSRAIYVQQLKGMLGGSGASVAIANLDFI
EFLNEVVESLGSSELKWSGTPEQLNLLPEADVALSPNTPDETAYLQFTSGSTRLPRGVVITERALMTNLRGIVCNGLDVR
PGDRCASWLPFYHDMGLVGLVLAPLAAQLSVDYLATRDFAVRPLQWLKLISRNRCTIAFSQPFGLKLCTLRARDSDLMDL
DLSCWRAAGVGAEMIRLDTLKSFSAKFSAAGFNEDAFLPCYGLAESTLAVSFPDINQGVKSLRVDAKTLIEKKIAVRVQA
DGRKFNEFVNCGRPLPGHEIRVVDDTDQEVPHLMVGSILVKGESVMNGYFNNAEETARAIRSDGWLDTGDIGFLFEGDLY
ITGRRTDVIIVNGRNIRAQDVEELAEQQPEVRTREASAFGISDENGSTSVVLVVECRLASAVDRQALINRLQQMVYMAFG
VSCLVELVPPHTLPRTSSGKLSRFAARQGFLRRTNMEQPAATSYTVG

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=580, Percent_Identity=24.4827586206897, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI44888818, Length=582, Percent_Identity=25.4295532646048, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI225735629, Length=502, Percent_Identity=24.3027888446215, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI45827692, Length=502, Percent_Identity=24.3027888446215, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693145, Length=407, Percent_Identity=27.7641277641278, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788107, Length=566, Percent_Identity=23.1448763250883, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI221142682, Length=170, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=562, Percent_Identity=22.7758007117438, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=563, Percent_Identity=22.5577264653641, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=563, Percent_Identity=22.5577264653641, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=563, Percent_Identity=22.7353463587922, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI193202679, Length=556, Percent_Identity=23.3812949640288, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=555, Percent_Identity=22.7027027027027, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=186, Percent_Identity=26.8817204301075, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581924, Length=400, Percent_Identity=25.75, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24654656, Length=603, Percent_Identity=25.3731343283582, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922652, Length=535, Percent_Identity=24.8598130841122, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648260, Length=178, Percent_Identity=30.3370786516854, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356947, Length=528, Percent_Identity=22.1590909090909, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653035, Length=182, Percent_Identity=29.1208791208791, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI161076582, Length=187, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=541, Percent_Identity=20.5175600739372, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648257, Length=183, Percent_Identity=28.9617486338798, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: NA

Molecular weight: Translated: 66223; Mature: 66091

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIIAKLDVLPAQEGNSLSSEAGVVSATPTINLLSQRRADFTTLVESLEYAAQGETGHN
CCCHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FYDAKGNLKSVLTYKNLCANAKIIARRLSGLGLARNSRVALIADTTPEFVELFFACRFAG
EECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHHHHHHC
LVPFAMPVPVNLGSRAIYVQQLKGMLGGSGASVAIANLDFIEFLNEVVESLGSSELKWSG
CCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECC
TPEQLNLLPEADVALSPNTPDETAYLQFTSGSTRLPRGVVITERALMTNLRGIVCNGLDV
CHHHHCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHCCCEEECCCCC
RPGDRCASWLPFYHDMGLVGLVLAPLAAQLSVDYLATRDFAVRPLQWLKLISRNRCTIAF
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEE
SQPFGLKLCTLRARDSDLMDLDLSCWRAAGVGAEMIRLDTLKSFSAKFSAAGFNEDAFLP
CCCCCEEEEEEECCCCCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
CYGLAESTLAVSFPDINQGVKSLRVDAKTLIEKKIAVRVQADGRKFNEFVNCGRPLPGHE
ECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCE
IRVVDDTDQEVPHLMVGSILVKGESVMNGYFNNAEETARAIRSDGWLDTGDIGFLFEGDL
EEEECCCCCCCCHHHHHHHEECCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCE
YITGRRTDVIIVNGRNIRAQDVEELAEQQPEVRTREASAFGISDENGSTSVVLVVECRLA
EEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEECCCCCCCCEEEEEEEEEHHH
SAVDRQALINRLQQMVYMAFGVSCLVELVPPHTLPRTSSGKLSRFAARQGFLRRTNMEQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
AATSYTVG
CCCCCCCC
>Mature Secondary Structure 
SSIIAKLDVLPAQEGNSLSSEAGVVSATPTINLLSQRRADFTTLVESLEYAAQGETGHN
CCHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FYDAKGNLKSVLTYKNLCANAKIIARRLSGLGLARNSRVALIADTTPEFVELFFACRFAG
EECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHHHHHHC
LVPFAMPVPVNLGSRAIYVQQLKGMLGGSGASVAIANLDFIEFLNEVVESLGSSELKWSG
CCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECC
TPEQLNLLPEADVALSPNTPDETAYLQFTSGSTRLPRGVVITERALMTNLRGIVCNGLDV
CHHHHCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHCCCEEECCCCC
RPGDRCASWLPFYHDMGLVGLVLAPLAAQLSVDYLATRDFAVRPLQWLKLISRNRCTIAF
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEE
SQPFGLKLCTLRARDSDLMDLDLSCWRAAGVGAEMIRLDTLKSFSAKFSAAGFNEDAFLP
CCCCCEEEEEEECCCCCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
CYGLAESTLAVSFPDINQGVKSLRVDAKTLIEKKIAVRVQADGRKFNEFVNCGRPLPGHE
ECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCE
IRVVDDTDQEVPHLMVGSILVKGESVMNGYFNNAEETARAIRSDGWLDTGDIGFLFEGDL
EEEECCCCCCCCHHHHHHHEECCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCE
YITGRRTDVIIVNGRNIRAQDVEELAEQQPEVRTREASAFGISDENGSTSVVLVVECRLA
EEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEECCCCCCCCEEEEEEEEEHHH
SAVDRQALINRLQQMVYMAFGVSCLVELVPPHTLPRTSSGKLSRFAARQGFLRRTNMEQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
AATSYTVG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]