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The map label for this gene is trmD [H]

Identifier: 114330463

GI number: 114330463

Start: 454912

End: 455757

Strand: Reverse

Name: trmD [H]

Synonym: Neut_0443

Alternate gene names: 114330463

Gene position: 455757-454912 (Counterclockwise)

Preceding gene: 114330464

Following gene: 114330462

Centisome position: 17.13

GC content: 45.63

Gene sequence:

>846_bases
ATGATGCCGTTTGAATTTGACGTCATTACGTTGTTGCCCGACATGTTTGATGCTGTCACACAACACGGAGTAACAGGCAG
AGCAAAAAAAAATAACTTATATCGTCTGCATACCTGGAACCCTCGCGACTATGCCACAAACCGTTATCGGACGGTAGATG
ATGCACCTTATGGTGGAGGTCCTGGCATGATCATGATGGCTGAACCGCTGGATCTGGCCATCACAGATGCAAAAATTCGG
CAGACCAGGAATGGAACCAGCAGAACCAGCGTCATTTACCTTTCCCCTCAGGGCAAGCGATTGGATCATAAAAAAATTCT
GCAGATTTCCAAACTAGATGGAGTCATATTGCTCTGTGGTCGTTACGAAGGAATTGATGAAAGATTAATAGAAGATCAGG
TAGACGAAGAAATTTCAATCGGGGATTACGTAATTTCTGGTGGGGAGCTGGCGGCCATGGTCTTAATTGATGCGGTGATC
AGGCAATTACCTGGCGCATTGGGTGATACGCAATCAGCCAGCCAAGATTCCCACGTTGATCATTTGCTGGAATATCCACA
CTATACCCGGCCCGATATCTATAAAGAAAAACCAGTACCCGAAGTGCTGTTGTCCGGTGACCACGCAAAAATAGAGCGCT
GGCGCCTGCAACAGGCAATTGGTAGAACTTGGCTAAAAAGGCCTGATTTGTTAGCAGAAAAATATCCAGAAGGCCTCCCT
GGCAAGGAAAAGGAATTACTGGAGGAGTTTAAGCAATTACAATGTTTGACGATTACCAACCAGGCGACAGATACAATAAA
AGAACAGGAGCAACGAAATGAACTTGATCGAACAACTGGAACGTGA

Upstream 100 bases:

>100_bases
AAAGAAGAATTGATTCCTTTTGTAGATCAGGTTGTCAGGCAAGTAGATATGAGATCACGACAAATCATGGTGGATTGGGA
GCTAGATTATTGAATTTTGT

Downstream 100 bases:

>100_bases
AGAAATAGAAAGGCTGGGGAAAATAATTCCCGATTTTTCGCCGGGCGATACACTCGTGGTCAACGTAAATGTCGTAGAAG
GTGATCGTAAACGTACCCAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MMPFEFDVITLLPDMFDAVTQHGVTGRAKKNNLYRLHTWNPRDYATNRYRTVDDAPYGGGPGMIMMAEPLDLAITDAKIR
QTRNGTSRTSVIYLSPQGKRLDHKKILQISKLDGVILLCGRYEGIDERLIEDQVDEEISIGDYVISGGELAAMVLIDAVI
RQLPGALGDTQSASQDSHVDHLLEYPHYTRPDIYKEKPVPEVLLSGDHAKIERWRLQQAIGRTWLKRPDLLAEKYPEGLP
GKEKELLEEFKQLQCLTITNQATDTIKEQEQRNELDRTTGT

Sequences:

>Translated_281_residues
MMPFEFDVITLLPDMFDAVTQHGVTGRAKKNNLYRLHTWNPRDYATNRYRTVDDAPYGGGPGMIMMAEPLDLAITDAKIR
QTRNGTSRTSVIYLSPQGKRLDHKKILQISKLDGVILLCGRYEGIDERLIEDQVDEEISIGDYVISGGELAAMVLIDAVI
RQLPGALGDTQSASQDSHVDHLLEYPHYTRPDIYKEKPVPEVLLSGDHAKIERWRLQQAIGRTWLKRPDLLAEKYPEGLP
GKEKELLEEFKQLQCLTITNQATDTIKEQEQRNELDRTTGT
>Mature_281_residues
MMPFEFDVITLLPDMFDAVTQHGVTGRAKKNNLYRLHTWNPRDYATNRYRTVDDAPYGGGPGMIMMAEPLDLAITDAKIR
QTRNGTSRTSVIYLSPQGKRLDHKKILQISKLDGVILLCGRYEGIDERLIEDQVDEEISIGDYVISGGELAAMVLIDAVI
RQLPGALGDTQSASQDSHVDHLLEYPHYTRPDIYKEKPVPEVLLSGDHAKIERWRLQQAIGRTWLKRPDLLAEKYPEGLP
GKEKELLEEFKQLQCLTITNQATDTIKEQEQRNELDRTTGT

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=53.2786885245902, Blast_Score=267, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 31888; Mature: 31888

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPFEFDVITLLPDMFDAVTQHGVTGRAKKNNLYRLHTWNPRDYATNRYRTVDDAPYGGG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCC
PGMIMMAEPLDLAITDAKIRQTRNGTSRTSVIYLSPQGKRLDHKKILQISKLDGVILLCG
CCEEEEECCCCEEEEHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEC
RYEGIDERLIEDQVDEEISIGDYVISGGELAAMVLIDAVIRQLPGALGDTQSASQDSHVD
CCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
HLLEYPHYTRPDIYKEKPVPEVLLSGDHAKIERWRLQQAIGRTWLKRPDLLAEKYPEGLP
HHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
GKEKELLEEFKQLQCLTITNQATDTIKEQEQRNELDRTTGT
CCHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMPFEFDVITLLPDMFDAVTQHGVTGRAKKNNLYRLHTWNPRDYATNRYRTVDDAPYGGG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCC
PGMIMMAEPLDLAITDAKIRQTRNGTSRTSVIYLSPQGKRLDHKKILQISKLDGVILLCG
CCEEEEECCCCEEEEHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEC
RYEGIDERLIEDQVDEEISIGDYVISGGELAAMVLIDAVIRQLPGALGDTQSASQDSHVD
CCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
HLLEYPHYTRPDIYKEKPVPEVLLSGDHAKIERWRLQQAIGRTWLKRPDLLAEKYPEGLP
HHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
GKEKELLEEFKQLQCLTITNQATDTIKEQEQRNELDRTTGT
CCHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]