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The map label for this gene is mdeA [H]

Identifier: 114330444

GI number: 114330444

Start: 439097

End: 440371

Strand: Direct

Name: mdeA [H]

Synonym: Neut_0424

Alternate gene names: 114330444

Gene position: 439097-440371 (Clockwise)

Preceding gene: 114330442

Following gene: 114330447

Centisome position: 16.5

GC content: 51.69

Gene sequence:

>1275_bases
ATGAAGCGAGAAACACTTGCCATTCATGGTGGTTTTGCCGGTGATCCACAGACACATTCGGTCGCAGTTCCTATTTATCA
GACCACCAGCTACTATTTTGACGATACCCAGCATGGGGCAGATCTGTTTGATCTCAAGGTACAAGGGAATATCTACACGC
GCATCATGAACCCGACTACTGCTGTGCTGGAAGAAAGAGTGGCATTACTGGAAGGGGGAGTGGGGGCGCTGGCGCTGGCT
TCCGGCATGGCGGCTATTACGGCCTGTGTCCAGACGCTGGCCAGGGCGGGCGATAACATCATTTCCACCAGCCAGGTTTA
TGGCGGAACCTACAATTTCTTTTGTCATACACTGCCCAATCAGGGAATTGAAGTGCGCATGGTGGATGGCCGTAATCCGG
CTGCTTTTGCAGATGCGATCGATGACAAGACCAGGATGGTTTTTTGTGAATCGATTGGCAACCCGGCCGGTAATGTGGTG
GACATTGCTGCATTGGCTGAAGCGGCACACACGGCGGGTGTGCCGCTGGTCGTGGATAACACTGTGGCTTCCCCGGCATT
GTGCCGTCCCTTTGAACATGGCGCCGATATTGTTGTCCATGCGCTGACAAAATACATGGGTGGTCACGGTACCAGCATTG
GCGGAATCATTGTGGATTCCGGCAAATTTCCCTGGAAAGACAGTCCGCGTTTTCCACAATTCAACAAGCCTGATCCCAGC
TATCACGGCGTGGTATATGTGGATGCATTTGGCCCGGCTGCGTTTATTGGTCGTGCGCGCGTTGTTCCGTTGCGCAACAT
GGGCGCGGCTATTTCTCCCTTTAATTCCTTCCTGATCCTGCAAGGAATTGAAACGCTACCACTGAGAATGGAGCGCCACT
GTTTCAATGCTTTGGAGATTGCACGTTACTTGAAAGCACACCCAAAAGTTGGCTGGGTTAACTTTGCCGGTCTTGAAGAC
AGCCGCGACTATGTTTTGGCACAAAAATACATGGATGGCGGTATTCCATCATCAATTCTAAGCTTTGGCATTAAAGGTGG
GCGTGATGCCTGTGCTCGCTTCATGGACAAGCTCGGGCTGATTAAACGACTGGTAAATATCGGGGACGCCAAAACGCTGG
CCTGCCACCCGGCTACCACGACTCACCGCCAACTTAATGATGAAGAGCTGGCCAAAGCTGGTGTCAGTATCGATTTGGTA
CGTTTGTGCGTCGGCATTGAGCATATTGATGATCTGATTGCCGATATAGACCAGGCCTTTCAGGAAGAAGTGTGA

Upstream 100 bases:

>100_bases
AATAACGAATATGGATTTATTCTGGAGTTCCTTTAAGATTGCCTGCATGTTATCCGGCCAACAGCCGGCACTCCAGTTTA
TTTTGAAAGAGGAAAATGTA

Downstream 100 bases:

>100_bases
GTATTTGTTTTTGTTGTATTACGTTGCTCGTCTGAAAGTGCGCTACGCAAGGTTTTGCTGGTGTCATCGGCTGGTGGGGT
TCTGGCCGGTTTTATACGTG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKRETLAIHGGFAGDPQTHSVAVPIYQTTSYYFDDTQHGADLFDLKVQGNIYTRIMNPTTAVLEERVALLEGGVGALALA
SGMAAITACVQTLARAGDNIISTSQVYGGTYNFFCHTLPNQGIEVRMVDGRNPAAFADAIDDKTRMVFCESIGNPAGNVV
DIAALAEAAHTAGVPLVVDNTVASPALCRPFEHGADIVVHALTKYMGGHGTSIGGIIVDSGKFPWKDSPRFPQFNKPDPS
YHGVVYVDAFGPAAFIGRARVVPLRNMGAAISPFNSFLILQGIETLPLRMERHCFNALEIARYLKAHPKVGWVNFAGLED
SRDYVLAQKYMDGGIPSSILSFGIKGGRDACARFMDKLGLIKRLVNIGDAKTLACHPATTTHRQLNDEELAKAGVSIDLV
RLCVGIEHIDDLIADIDQAFQEEV

Sequences:

>Translated_424_residues
MKRETLAIHGGFAGDPQTHSVAVPIYQTTSYYFDDTQHGADLFDLKVQGNIYTRIMNPTTAVLEERVALLEGGVGALALA
SGMAAITACVQTLARAGDNIISTSQVYGGTYNFFCHTLPNQGIEVRMVDGRNPAAFADAIDDKTRMVFCESIGNPAGNVV
DIAALAEAAHTAGVPLVVDNTVASPALCRPFEHGADIVVHALTKYMGGHGTSIGGIIVDSGKFPWKDSPRFPQFNKPDPS
YHGVVYVDAFGPAAFIGRARVVPLRNMGAAISPFNSFLILQGIETLPLRMERHCFNALEIARYLKAHPKVGWVNFAGLED
SRDYVLAQKYMDGGIPSSILSFGIKGGRDACARFMDKLGLIKRLVNIGDAKTLACHPATTTHRQLNDEELAKAGVSIDLV
RLCVGIEHIDDLIADIDQAFQEEV
>Mature_424_residues
MKRETLAIHGGFAGDPQTHSVAVPIYQTTSYYFDDTQHGADLFDLKVQGNIYTRIMNPTTAVLEERVALLEGGVGALALA
SGMAAITACVQTLARAGDNIISTSQVYGGTYNFFCHTLPNQGIEVRMVDGRNPAAFADAIDDKTRMVFCESIGNPAGNVV
DIAALAEAAHTAGVPLVVDNTVASPALCRPFEHGADIVVHALTKYMGGHGTSIGGIIVDSGKFPWKDSPRFPQFNKPDPS
YHGVVYVDAFGPAAFIGRARVVPLRNMGAAISPFNSFLILQGIETLPLRMERHCFNALEIARYLKAHPKVGWVNFAGLED
SRDYVLAQKYMDGGIPSSILSFGIKGGRDACARFMDKLGLIKRLVNIGDAKTLACHPATTTHRQLNDEELAKAGVSIDLV
RLCVGIEHIDDLIADIDQAFQEEV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=405, Percent_Identity=31.358024691358, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=27.790973871734, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=29.0476190476191, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=31.1904761904762, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=24.1217798594848, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=29.6116504854369, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17538055, Length=407, Percent_Identity=32.1867321867322, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=407, Percent_Identity=31.4496314496315, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=50, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=32.4766355140187, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=27.6018099547511, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=427, Percent_Identity=25.7611241217799, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=203, Percent_Identity=32.0197044334975, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=33.806146572104, Blast_Score=178, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45674; Mature: 45674

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRETLAIHGGFAGDPQTHSVAVPIYQTTSYYFDDTQHGADLFDLKVQGNIYTRIMNPTT
CCCCEEEEECCCCCCCCCCEEEEEEEECCEEEECCCCCCCCEEEEEECCCEEEEECCCHH
AVLEERVALLEGGVGALALASGMAAITACVQTLARAGDNIISTSQVYGGTYNFFCHTLPN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHCCCEEEEEEEECCC
QGIEVRMVDGRNPAAFADAIDDKTRMVFCESIGNPAGNVVDIAALAEAAHTAGVPLVVDN
CCEEEEEECCCCCHHHHHHCCCCCEEEEEHHCCCCCCCEEHHHHHHHHHHHCCCCEEECC
TVASPALCRPFEHGADIVVHALTKYMGGHGTSIGGIIVDSGKFPWKDSPRFPQFNKPDPS
CCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
YHGVVYVDAFGPAAFIGRARVVPLRNMGAAISPFNSFLILQGIETLPLRMERHCFNALEI
CCCEEEEECCCCHHHHCCEEEEEHHCCCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHH
ARYLKAHPKVGWVNFAGLEDSRDYVLAQKYMDGGIPSSILSFGIKGGRDACARFMDKLGL
HHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
IKRLVNIGDAKTLACHPATTTHRQLNDEELAKAGVSIDLVRLCVGIEHIDDLIADIDQAF
HHHHHHCCCCCEEEECCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QEEV
HHCC
>Mature Secondary Structure
MKRETLAIHGGFAGDPQTHSVAVPIYQTTSYYFDDTQHGADLFDLKVQGNIYTRIMNPTT
CCCCEEEEECCCCCCCCCCEEEEEEEECCEEEECCCCCCCCEEEEEECCCEEEEECCCHH
AVLEERVALLEGGVGALALASGMAAITACVQTLARAGDNIISTSQVYGGTYNFFCHTLPN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHCCCEEEEEEEECCC
QGIEVRMVDGRNPAAFADAIDDKTRMVFCESIGNPAGNVVDIAALAEAAHTAGVPLVVDN
CCEEEEEECCCCCHHHHHHCCCCCEEEEEHHCCCCCCCEEHHHHHHHHHHHCCCCEEECC
TVASPALCRPFEHGADIVVHALTKYMGGHGTSIGGIIVDSGKFPWKDSPRFPQFNKPDPS
CCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
YHGVVYVDAFGPAAFIGRARVVPLRNMGAAISPFNSFLILQGIETLPLRMERHCFNALEI
CCCEEEEECCCCHHHHCCEEEEEHHCCCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHH
ARYLKAHPKVGWVNFAGLEDSRDYVLAQKYMDGGIPSSILSFGIKGGRDACARFMDKLGL
HHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
IKRLVNIGDAKTLACHPATTTHRQLNDEELAKAGVSIDLVRLCVGIEHIDDLIADIDQAF
HHHHHHCCCCCEEEECCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QEEV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]