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The map label for this gene is miaA

Identifier: 114330407

GI number: 114330407

Start: 389884

End: 390837

Strand: Direct

Name: miaA

Synonym: Neut_0386

Alternate gene names: 114330407

Gene position: 389884-390837 (Clockwise)

Preceding gene: 114330405

Following gene: 114330414

Centisome position: 14.65

GC content: 48.43

Gene sequence:

>954_bases
GTGAATCTTCCTGATACAGAATCTCCAGCAGCCATTTTTCTGATGGGGCCTACTGCCAGCGGTAAGTCTGGCTTGGCAAT
AGAAATCGCACGTCACTTCCCGGTAGAAGTTGTTAGTGTTGATTCAGCGCAGGTTTACCGGTATATGGATATCGGCAGCG
CCAAGCCGGACAAATCGATTCAGATAGAAATCCCGCATCATTTGATTAATTTGATTAACCCTGATGAAAGTTATTCTGCT
GCGCAATTCAGGGAGGATGCCTTGTCTGTCATGCATGGGATTACAGCACGCGGCAGAATACCGCTGTTAGTTGGCGGTAC
CATGCTTTATTTCAAGGTGTTGCAACAAGGATTGGCCACACTGCCTGCAGCTGATGAGGCTGTGCGCAAGGAGCTGGAAC
AATCAGCCCGCGAACAAGGATGGCCTGCTATGCATACTGTTCTGAGTCGGCTTGATCCTGTCATAGCAGAACGCATCAAA
CCGAATGACAGTCAGCGTATTCAGCGCGCACTGGAAGTCTGTTATCTGACTGGCAGACCTATGTCAGCAGTGCTGAAACA
ACAGCAGAGCCGTGATTTTCCATATCGTGTTTTTAATATCGCTCTACTTCCGGGTGATCGCAGCGTTTTACATGCTCGCA
TCAGTCAGCGGTTTGACAAAATGCTGGAAATAGGGTTGGTGGATGAAGTACGTATGATCCGTGATCAATTCCAGGTGACC
GCTGATATGCCTGCAATGCGTTGCGTCGGATATCGTCAGGTATATATGTATCTGGAGAATGAAATCAGCTTGGCTGAAAT
GCGGGAGAGGGGTGTTTTTGCAACCAGACAGCTGGCCAAGCGCCAATTAACCTGGCTGAGAGCAATGAATGAACTGCATG
GCTTTGACTGCCTGGCTAATCAGTTAGCCCAACAGATTATCGGCTTTATACAGAAGCAGCGTATCTTTGCCTGA

Upstream 100 bases:

>100_bases
TTGTTGTCATTTGAAAACTTCAGCTGACAACATTAAAAATCATGATTCGAATAACCGATCGGTTGTTCACACTTTTTATT
GTAACTGATAACAGTTGGCC

Downstream 100 bases:

>100_bases
GCAGTGTTTCAGCAGTGGTGGTTGTCTGTTTTGTGCCTGCAACTGGAATGATGTTTGCATCCCGCAGCTACAACGCTACA
CCCACGTCGGAATTGTATAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNLPDTESPAAIFLMGPTASGKSGLAIEIARHFPVEVVSVDSAQVYRYMDIGSAKPDKSIQIEIPHHLINLINPDESYSA
AQFREDALSVMHGITARGRIPLLVGGTMLYFKVLQQGLATLPAADEAVRKELEQSAREQGWPAMHTVLSRLDPVIAERIK
PNDSQRIQRALEVCYLTGRPMSAVLKQQQSRDFPYRVFNIALLPGDRSVLHARISQRFDKMLEIGLVDEVRMIRDQFQVT
ADMPAMRCVGYRQVYMYLENEISLAEMRERGVFATRQLAKRQLTWLRAMNELHGFDCLANQLAQQIIGFIQKQRIFA

Sequences:

>Translated_317_residues
MNLPDTESPAAIFLMGPTASGKSGLAIEIARHFPVEVVSVDSAQVYRYMDIGSAKPDKSIQIEIPHHLINLINPDESYSA
AQFREDALSVMHGITARGRIPLLVGGTMLYFKVLQQGLATLPAADEAVRKELEQSAREQGWPAMHTVLSRLDPVIAERIK
PNDSQRIQRALEVCYLTGRPMSAVLKQQQSRDFPYRVFNIALLPGDRSVLHARISQRFDKMLEIGLVDEVRMIRDQFQVT
ADMPAMRCVGYRQVYMYLENEISLAEMRERGVFATRQLAKRQLTWLRAMNELHGFDCLANQLAQQIIGFIQKQRIFA
>Mature_317_residues
MNLPDTESPAAIFLMGPTASGKSGLAIEIARHFPVEVVSVDSAQVYRYMDIGSAKPDKSIQIEIPHHLINLINPDESYSA
AQFREDALSVMHGITARGRIPLLVGGTMLYFKVLQQGLATLPAADEAVRKELEQSAREQGWPAMHTVLSRLDPVIAERIK
PNDSQRIQRALEVCYLTGRPMSAVLKQQQSRDFPYRVFNIALLPGDRSVLHARISQRFDKMLEIGLVDEVRMIRDQFQVT
ADMPAMRCVGYRQVYMYLENEISLAEMRERGVFATRQLAKRQLTWLRAMNELHGFDCLANQLAQQIIGFIQKQRIFA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=29.903536977492, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=52.2875816993464, Blast_Score=318, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI25144712, Length=272, Percent_Identity=29.7794117647059, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=30.4054054054054, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24649830, Length=268, Percent_Identity=30.2238805970149, Blast_Score=121, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_NITEC (Q0AJ07)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746629.1
- ProteinModelPortal:   Q0AJ07
- SMR:   Q0AJ07
- STRING:   Q0AJ07
- GeneID:   4273446
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0386
- NMPDR:   fig|335283.3.peg.1982
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q0AJ07
- ProtClustDB:   PRK00091
- BioCyc:   NEUT335283:NEUT_0386-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35883; Mature: 35883

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPDTESPAAIFLMGPTASGKSGLAIEIARHFPVEVVSVDSAQVYRYMDIGSAKPDKSI
CCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEECCHHHHHHEEECCCCCCCCCE
QIEIPHHLINLINPDESYSAAQFREDALSVMHGITARGRIPLLVGGTMLYFKVLQQGLAT
EEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHC
LPAADEAVRKELEQSAREQGWPAMHTVLSRLDPVIAERIKPNDSQRIQRALEVCYLTGRP
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
MSAVLKQQQSRDFPYRVFNIALLPGDRSVLHARISQRFDKMLEIGLVDEVRMIRDQFQVT
HHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ADMPAMRCVGYRQVYMYLENEISLAEMRERGVFATRQLAKRQLTWLRAMNELHGFDCLAN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QLAQQIIGFIQKQRIFA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLPDTESPAAIFLMGPTASGKSGLAIEIARHFPVEVVSVDSAQVYRYMDIGSAKPDKSI
CCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEECCHHHHHHEEECCCCCCCCCE
QIEIPHHLINLINPDESYSAAQFREDALSVMHGITARGRIPLLVGGTMLYFKVLQQGLAT
EEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHC
LPAADEAVRKELEQSAREQGWPAMHTVLSRLDPVIAERIKPNDSQRIQRALEVCYLTGRP
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
MSAVLKQQQSRDFPYRVFNIALLPGDRSVLHARISQRFDKMLEIGLVDEVRMIRDQFQVT
HHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ADMPAMRCVGYRQVYMYLENEISLAEMRERGVFATRQLAKRQLTWLRAMNELHGFDCLAN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QLAQQIIGFIQKQRIFA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA