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The map label for this gene is gap [H]

Identifier: 114330356

GI number: 114330356

Start: 339806

End: 340813

Strand: Reverse

Name: gap [H]

Synonym: Neut_0333

Alternate gene names: 114330356

Gene position: 340813-339806 (Counterclockwise)

Preceding gene: 114330357

Following gene: 114330355

Centisome position: 12.81

GC content: 52.78

Gene sequence:

>1008_bases
ATGGCGATCAAAATTGGTATCAACGGCTTCGGACGAATCGGTCGCTGCGTTGCACGCATTGCGGCTCAGAACCCTAGCGT
CGACCTTGTCGCAATTAACGATCTGACAAGCGCCGAGCAGCTTGCGTACCTCTTTAAGTACGACTCAACGCATGGCAAAT
ACGCGGGCGTGGTCAGCAGCACCGAAAACTTGATCAGCGTCGACGGTGACAAGATACGCGTTTTTTCTGAAAGAGACCCC
TCCAATATTCCATGGGGCGATCTTGGTGTGGATTATGTGCTGGAATGCACGGGTATTTTCCGCAATAAAGAGCAGGTTTC
GAAACACATCGAAGCTGGCGCAAAATTTGTGCTCATGTCGGCTCCCGGCAAGGGCCTCGACATGACTTTTGTGTTGGGCG
TGAACGAAGAAGGTTTCACTCCTGATATGACTGTGATCGACGTCGCGAGCTGCACTACCAATTGCCTGGCTCCAGTTGCA
AAGGTCCTGCATGAAAACTTCGGTATTCAGAACGGCCTCATGACAACAATTCACTCGTACACAAACGACCAAGGCCTCCT
GGATGCACCGCATAAGTCGGACTTCCGCCGTGCTCGCGCAGCCGCTGTGAACATGGTCATCACCACCACTGGCGCAGCCG
TTGCCGTGACCAAGGCGATGCCTGAGCTCAAAGGCAAACTCGACGGCATGTCGGTTCGCGTTCCAACACCCAATGTTTCG
TGCGTTGATCTTGTCGCGACCTTCGAAAAGCCTGTGACGGTCGAATCCATCAACGCTGCAATGAAAAAAGCCGCGCAAAC
GACTTTGAACGGCATCCTTGCTTACACCGAAGACCCGGTCGTCTCGACAGACATGATGAACGACCCTCATTCATCGATCT
TCGATTCGAGAGCGACCATGGTGATCGGCACACATATGGCCAAGACCATCTCGTGGTACGACAATGAATGGGGTTATTCG
GCCCGCATGATCGACGCGCTTCTTTTTGTCGACAACAAGCGGAAATAA

Upstream 100 bases:

>100_bases
TTAAGAGGATGGCATGTCAGCTCATCCAAAATATGCGTTTTGCGCCTAATGCGACAGAACCGTTTATTTTTCCTATACAC
CTAACCAGCACGAGGACATC

Downstream 100 bases:

>100_bases
TCCATAGATACCGGCATTTCATTTATCGACCAGCCTGAGCTGGTTGATAAAAGGAGTTCTGTCACAAATATTCGTCAAGT
GCAAAGCCACAGTTTGTGTC

Product: glyceraldehyde-3-phosphate dehydrogenase, type I

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAIKIGINGFGRIGRCVARIAAQNPSVDLVAINDLTSAEQLAYLFKYDSTHGKYAGVVSSTENLISVDGDKIRVFSERDP
SNIPWGDLGVDYVLECTGIFRNKEQVSKHIEAGAKFVLMSAPGKGLDMTFVLGVNEEGFTPDMTVIDVASCTTNCLAPVA
KVLHENFGIQNGLMTTIHSYTNDQGLLDAPHKSDFRRARAAAVNMVITTTGAAVAVTKAMPELKGKLDGMSVRVPTPNVS
CVDLVATFEKPVTVESINAAMKKAAQTTLNGILAYTEDPVVSTDMMNDPHSSIFDSRATMVIGTHMAKTISWYDNEWGYS
ARMIDALLFVDNKRK

Sequences:

>Translated_335_residues
MAIKIGINGFGRIGRCVARIAAQNPSVDLVAINDLTSAEQLAYLFKYDSTHGKYAGVVSSTENLISVDGDKIRVFSERDP
SNIPWGDLGVDYVLECTGIFRNKEQVSKHIEAGAKFVLMSAPGKGLDMTFVLGVNEEGFTPDMTVIDVASCTTNCLAPVA
KVLHENFGIQNGLMTTIHSYTNDQGLLDAPHKSDFRRARAAAVNMVITTTGAAVAVTKAMPELKGKLDGMSVRVPTPNVS
CVDLVATFEKPVTVESINAAMKKAAQTTLNGILAYTEDPVVSTDMMNDPHSSIFDSRATMVIGTHMAKTISWYDNEWGYS
ARMIDALLFVDNKRK
>Mature_334_residues
AIKIGINGFGRIGRCVARIAAQNPSVDLVAINDLTSAEQLAYLFKYDSTHGKYAGVVSSTENLISVDGDKIRVFSERDPS
NIPWGDLGVDYVLECTGIFRNKEQVSKHIEAGAKFVLMSAPGKGLDMTFVLGVNEEGFTPDMTVIDVASCTTNCLAPVAK
VLHENFGIQNGLMTTIHSYTNDQGLLDAPHKSDFRRARAAAVNMVITTTGAAVAVTKAMPELKGKLDGMSVRVPTPNVSC
VDLVATFEKPVTVESINAAMKKAAQTTLNGILAYTEDPVVSTDMMNDPHSSIFDSRATMVIGTHMAKTISWYDNEWGYSA
RMIDALLFVDNKRK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=48.948948948949, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI7657116, Length=335, Percent_Identity=49.8507462686567, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1788079, Length=330, Percent_Identity=49.6969696969697, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1789295, Length=332, Percent_Identity=44.2771084337349, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17534679, Length=333, Percent_Identity=48.948948948949, Blast_Score=326, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI17534677, Length=333, Percent_Identity=48.6486486486487, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=49.1071428571429, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=49.1071428571429, Blast_Score=325, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6321631, Length=324, Percent_Identity=51.2345679012346, Blast_Score=348, Evalue=5e-97,
Organism=Saccharomyces cerevisiae, GI6322409, Length=329, Percent_Identity=50.4559270516717, Blast_Score=347, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6322468, Length=324, Percent_Identity=51.2345679012346, Blast_Score=346, Evalue=4e-96,
Organism=Drosophila melanogaster, GI17933600, Length=331, Percent_Identity=49.5468277945619, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI18110149, Length=331, Percent_Identity=49.5468277945619, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=49.8489425981873, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=49.8489425981873, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=46.6257668711656, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36264; Mature: 36133

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKIGINGFGRIGRCVARIAAQNPSVDLVAINDLTSAEQLAYLFKYDSTHGKYAGVVSS
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEEEEECCCCCCEEEEECC
TENLISVDGDKIRVFSERDPSNIPWGDLGVDYVLECTGIFRNKEQVSKHIEAGAKFVLMS
CCCEEEECCCEEEEEECCCCCCCCCCCCCHHHHEEHHHHHCCHHHHHHHHHCCCEEEEEE
APGKGLDMTFVLGVNEEGFTPDMTVIDVASCTTNCLAPVAKVLHENFGIQNGLMTTIHSY
CCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TNDQGLLDAPHKSDFRRARAAAVNMVITTTGAAVAVTKAMPELKGKLDGMSVRVPTPNVS
CCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCH
CVDLVATFEKPVTVESINAAMKKAAQTTLNGILAYTEDPVVSTDMMNDPHSSIFDSRATM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCCCHHHHCCCCEE
VIGTHMAKTISWYDNEWGYSARMIDALLFVDNKRK
EEEHHHHHHHHHCCCCCCCHHHHHHHEEEECCCCC
>Mature Secondary Structure 
AIKIGINGFGRIGRCVARIAAQNPSVDLVAINDLTSAEQLAYLFKYDSTHGKYAGVVSS
EEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEEEEECCCCCCEEEEECC
TENLISVDGDKIRVFSERDPSNIPWGDLGVDYVLECTGIFRNKEQVSKHIEAGAKFVLMS
CCCEEEECCCEEEEEECCCCCCCCCCCCCHHHHEEHHHHHCCHHHHHHHHHCCCEEEEEE
APGKGLDMTFVLGVNEEGFTPDMTVIDVASCTTNCLAPVAKVLHENFGIQNGLMTTIHSY
CCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TNDQGLLDAPHKSDFRRARAAAVNMVITTTGAAVAVTKAMPELKGKLDGMSVRVPTPNVS
CCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCH
CVDLVATFEKPVTVESINAAMKKAAQTTLNGILAYTEDPVVSTDMMNDPHSSIFDSRATM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCCCHHHHCCCCEE
VIGTHMAKTISWYDNEWGYSARMIDALLFVDNKRK
EEEHHHHHHHHHCCCCCCCHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]