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The map label for this gene is kefB [H]

Identifier: 114330343

GI number: 114330343

Start: 324955

End: 326661

Strand: Direct

Name: kefB [H]

Synonym: Neut_0318

Alternate gene names: 114330343

Gene position: 324955-326661 (Clockwise)

Preceding gene: 114330341

Following gene: 114330344

Centisome position: 12.21

GC content: 54.01

Gene sequence:

>1707_bases
ATGATGGAGTATCTCGTTCAGATTATTCTACTGCTGGCCGTTGCCATCACTGTGGTGGTGGTTTTTCAGCGGCTGCATAT
CCCCACCAGCCTGGGTTACCTACTGGTTGGAATCCTGCTGGGGCCCCATACAGCTGGGCCTGTGGTCTCTATGCCTGGGC
TGGAATCGCTCGCGGAGTTCGGTGTTGTTTTCTTGTTGTTTACCATTGGGCTTAACTTTTCCCTGCCGCAAATGGATGCG
CTGAGAACCCGGGTTCTCGGACTGGGTACCGGGCAAGTCGTGCTTACCACCTTGGTGGTGGGCGTTGTTACCTGGCTTGC
CGGGCTTCCTATCATTGCAGCATTCGTGTTTGGTGCGGTGTTTGCCCAGTCCTCCACCACTATTATTGCCAGCCTGCTCA
CTGAACAAGGTGAGGTAAATACGCCCCACGGCCGTCTGGGTCTGGCCATGTCAGTGTTTCAGGATGTCACTGCCGTTCCA
TTTCTGGTGGTGATCCCGGTTCTAGGTGTATCCGTGGCCGCCGATGTGCTGGCTGGATCCCTGGCTCTGGCTTTTGCCAA
AGCGGTGTTGGCTTTCATTCTGGTGTTCATCGTCGGGCGCTGGCTATTATCCCCATTTTTTCAGATTATTTCTGAACGCA
GGTCAGTTGAGGTATTTACGCTGGCCGTGTTGCTGGTGGCCCTGTTTGCCGCATGGATTACTAACAGCTTGGGCTTGTCA
CTGGCCTTCGGTGGATTCCTTGCCGGCCTTGTTCTGGGTAAAACAGAGTTTCGCCATCAGGTTGAGTCCAGTATCAGCCC
GTTCCGGGATGTGTTGCTGGGCCTTTTCTTTATTGGTATTGGCATGCGTTTCAACCCGGTGGCGATACCGCCTATCTGGC
AGTGGACACTGCTAGGCGCCTTGCTTATTTTGATCAGCAAGATCCTGATTGTTGCCGCATTGGTACGCGGGATTGGGGTT
GATGCCAGGATGATGTGGCGCACGGGACTAATGTTGAGTGTGGGTGGTGAGTTCGGGCTTGCCCTCATTGCAATTGCACT
TGATTCTGGTGTGATTGACATCGGGCTTGGTCAGATCGCGATATCATCCGTACTTCTGTCGATGGTTTTGGGTGCTGTGC
TGATCCGCTTCAATGATGCCATCGCTACTTTCTTGACTAAGACTTCACCGCCATCTGATGAGGAGGCAGCCGAGTTGCCG
CAAGCGGCCGAGCGTTCCGTGATAATTGGTGGCTACGGGCGCATGGGGCGTACGATTGCTATTTTATTGCATGCATGCGG
TATACCGTTTGTGGCGTTTGATACGGATCCCCGACGGGTGGCCCTGGGCCGGGCGGATCGACATGCGGTTTGGCTCGGTG
ATATTGCCGATCCCATATTACTGGCCTCCATTCGGGTGGAGCGGGCGTCTCTGATCGTGCTGGCAGTTGATATCAAGAGC
ACCGCATTAAGTGCCATGATCTATATAAAAAGAACCAGTGCCCATGTTCCGGTGATTGTACGGGTACGGGACCTGGAAAC
CAGCCGGCATTTGCTAGCGGCCGGTGCTGCCCATGCTTACCCGGAAACAATTGAATCGAGTCTGAGCCTTGGGGTGACAG
CGCTGCGGATGCTCAATGTTGCGGCCGATGAAATTGAGGAGGCCGTTCAAAATCTGAGAAACCGAGGTTATCAGCCCGTT
GTGGAAGATGAAAAAAAGAGGTTGTGA

Upstream 100 bases:

>100_bases
CTGAACAAATAACTCTTTAACTTCGGTACTAGGGTATCCAACTATTCGATTCCACTTGTTGTAGTAGCCTATGGCAGGCC
TTGCACGGTAAACTCCAGCC

Downstream 100 bases:

>100_bases
GAGCGTTCATACTTCACCACACAGCCAACGGGTGAGGTGTGTTGTCAAAAATGGAGTCCATGTGTTTAAGCGGCGATCAG
ATTTGCATAACATTGTTATT

Product: sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MMEYLVQIILLLAVAITVVVVFQRLHIPTSLGYLLVGILLGPHTAGPVVSMPGLESLAEFGVVFLLFTIGLNFSLPQMDA
LRTRVLGLGTGQVVLTTLVVGVVTWLAGLPIIAAFVFGAVFAQSSTTIIASLLTEQGEVNTPHGRLGLAMSVFQDVTAVP
FLVVIPVLGVSVAADVLAGSLALAFAKAVLAFILVFIVGRWLLSPFFQIISERRSVEVFTLAVLLVALFAAWITNSLGLS
LAFGGFLAGLVLGKTEFRHQVESSISPFRDVLLGLFFIGIGMRFNPVAIPPIWQWTLLGALLILISKILIVAALVRGIGV
DARMMWRTGLMLSVGGEFGLALIAIALDSGVIDIGLGQIAISSVLLSMVLGAVLIRFNDAIATFLTKTSPPSDEEAAELP
QAAERSVIIGGYGRMGRTIAILLHACGIPFVAFDTDPRRVALGRADRHAVWLGDIADPILLASIRVERASLIVLAVDIKS
TALSAMIYIKRTSAHVPVIVRVRDLETSRHLLAAGAAHAYPETIESSLSLGVTALRMLNVAADEIEEAVQNLRNRGYQPV
VEDEKKRL

Sequences:

>Translated_568_residues
MMEYLVQIILLLAVAITVVVVFQRLHIPTSLGYLLVGILLGPHTAGPVVSMPGLESLAEFGVVFLLFTIGLNFSLPQMDA
LRTRVLGLGTGQVVLTTLVVGVVTWLAGLPIIAAFVFGAVFAQSSTTIIASLLTEQGEVNTPHGRLGLAMSVFQDVTAVP
FLVVIPVLGVSVAADVLAGSLALAFAKAVLAFILVFIVGRWLLSPFFQIISERRSVEVFTLAVLLVALFAAWITNSLGLS
LAFGGFLAGLVLGKTEFRHQVESSISPFRDVLLGLFFIGIGMRFNPVAIPPIWQWTLLGALLILISKILIVAALVRGIGV
DARMMWRTGLMLSVGGEFGLALIAIALDSGVIDIGLGQIAISSVLLSMVLGAVLIRFNDAIATFLTKTSPPSDEEAAELP
QAAERSVIIGGYGRMGRTIAILLHACGIPFVAFDTDPRRVALGRADRHAVWLGDIADPILLASIRVERASLIVLAVDIKS
TALSAMIYIKRTSAHVPVIVRVRDLETSRHLLAAGAAHAYPETIESSLSLGVTALRMLNVAADEIEEAVQNLRNRGYQPV
VEDEKKRL
>Mature_568_residues
MMEYLVQIILLLAVAITVVVVFQRLHIPTSLGYLLVGILLGPHTAGPVVSMPGLESLAEFGVVFLLFTIGLNFSLPQMDA
LRTRVLGLGTGQVVLTTLVVGVVTWLAGLPIIAAFVFGAVFAQSSTTIIASLLTEQGEVNTPHGRLGLAMSVFQDVTAVP
FLVVIPVLGVSVAADVLAGSLALAFAKAVLAFILVFIVGRWLLSPFFQIISERRSVEVFTLAVLLVALFAAWITNSLGLS
LAFGGFLAGLVLGKTEFRHQVESSISPFRDVLLGLFFIGIGMRFNPVAIPPIWQWTLLGALLILISKILIVAALVRGIGV
DARMMWRTGLMLSVGGEFGLALIAIALDSGVIDIGLGQIAISSVLLSMVLGAVLIRFNDAIATFLTKTSPPSDEEAAELP
QAAERSVIIGGYGRMGRTIAILLHACGIPFVAFDTDPRRVALGRADRHAVWLGDIADPILLASIRVERASLIVLAVDIKS
TALSAMIYIKRTSAHVPVIVRVRDLETSRHLLAAGAAHAYPETIESSLSLGVTALRMLNVAADEIEEAVQNLRNRGYQPV
VEDEKKRL

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=286, Percent_Identity=26.9230769230769, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786232, Length=565, Percent_Identity=31.6814159292035, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1789749, Length=552, Percent_Identity=31.3405797101449, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786685, Length=506, Percent_Identity=28.4584980237154, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 60607; Mature: 60607

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA