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The map label for this gene is dnaG [H]

Identifier: 114330326

GI number: 114330326

Start: 305465

End: 307258

Strand: Direct

Name: dnaG [H]

Synonym: Neut_0299

Alternate gene names: 114330326

Gene position: 305465-307258 (Clockwise)

Preceding gene: 114330325

Following gene: 114330327

Centisome position: 11.48

GC content: 42.92

Gene sequence:

>1794_bases
ATGATTGCGCAGCCATTTATTCAGGAATTGTTGGATCGTATCGATATTGTCGATATTGTTGCAAGACATCTTAAACTCAA
AAAGGCCGGTGCAAATTTTACAGCGTGCTGTCCTTTCCATAATGAAAAAACCCCGTCATTTACTGTAAATTCGTCCAAGC
AGTTTTACCATTGCTTTGGTTGTGGCAGGCACGGCAATGCCATTAATTTTTTGATGGAACATAGTGGAGTCAGTTTTGTC
GAAGCAGTGGAAAGTCTCGCGGCGCATGCTGGTATGCAAATTCCTGCTTGGAAGAACATTAATTCAAAAGCATCCGATTC
AGGTGAAACTTCATTGAATGAAGTGAGATTTGATGAGGTTATACAGACATCATCTGATTTGTATGGGCGGATGGAGCAGG
CTGCAAAGTTTTATCGTGTGCAACTCAAACAATCAGAGTTGGCGATTGCTTATTTGAAAGATCGAGGGGTGTCAGGGCAG
ACCGCTTTGCATTTCGGAATCGGATACGCACCTTCTGGCTGGCAAAATCTGGCTGAGATCTTTACGGATTATCCGTCAGG
TGATTCCAGTCATGCGCTGGTGCGATCAGGTTTGATCGTTGCTCATGAAAGCAAAAAAAACTATGACCGTTTCCGTCATC
GGATCATGTTCCCTATTCTCGATCTTAAAAAGAGAATAATAGGGTTTGGCGGACGAGCATTGGATGGGAGTGAACCGAAG
TACCTCAATTCGCCGGAAACTCCGTTATTTATCAAAGGACGCGAACTTTATAATCTGGTATCAGCCGGTCCGGCAATCCG
AAAATCAGGTCGGGTAGTCGTCGTAGAAGGTTATATGGACGTTGTAATGCTGGTGCAGCACGGCATTGAAAACGTAGTGG
CAACTTTAGGTACGGCAACGACGTCTACGCATATCCAGAAGCTTTTACGTTATACGGATGAAGTGGTTTTTTGTTTTGAT
GGAGATGAAGCCGGTACAAAAGCTGCTTGGCGAGCATTAGAAACCAGTTTGCCGCAACTCAAAGATGGGAAGGATATCAA
ATTCTTATTTCTACCGGATAAAGAAGATCCAGATAGCTATATTCGCAAATACGGCAAGGATGCTTTTAAAAATCTGCTGG
AAAAAGCTCAGCTGCTATCTGTGTTTTTTTGTAAAGAGTTATCTAGCCGTGTGAATCTTAGTACAAGTGAGGGACGTGCC
CGATTGGTTCAGCAGGCAGAACCGCTGCTTGTGCAGATTAATGCTCCTATATTTGGATTTATGTTAATGAAGCGTATTTC
TGAGTTAGCTGGAGTGGATCAGAATCAGCTGATTGCTTTTCTGAAACCCGGAAAGAAGAATCAGCCTTCCATATCTCGTT
CAAATGTATCCAGGCCCTTATCGGTTACGCCATATCGAAGGTTGATACAGATTTTGCTGCACACTCCAGCTTATGTGGAT
AAGCTGGATACAGATCTTTTTGCATTAAATGATGGTCAAAATGAGGAAAAGGCACTGCTGGCTGCATTGGTGGATTTTCT
TAAAACATCTTCCTGCACTTCGATGGATGAATTGAATTCAGTAGCAATCATGTTGCATCTTGATCAAACTCCGCATCGTG
TTTTATTGGAAAAGATTGCCCGGGATATACATGTAAAAGATGTAGATTGGGATATCGATGCTGAATTTACTGGAGCAATG
GTGCGATTACGAGAAGCTCAACGTAAGTCGAGAGTGGCTGAGTTACACAATAGACCGCTTGCATCACTTACTCCGGAAGA
GAAAAAAGAGTTGCGACGATTGATACTTTCTTGA

Upstream 100 bases:

>100_bases
AATTAAGTTGAAGCTAACCGGCTAATACCTGTTCCAGGCAGGCATACTTCAGAAGAGGTGTGCTCCGAGTGGTCTTTTAT
CGGAGATTTATTACCCATAA

Downstream 100 bases:

>100_bases
GCTGTGGTGTGCAGGACCTTTTACAAGGTGATCAAAATAAAAAATGTGGGGTATATCAAATTGTGTTGCCGCGATTGGTT
GATAAGCGATACTTTTGTTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MIAQPFIQELLDRIDIVDIVARHLKLKKAGANFTACCPFHNEKTPSFTVNSSKQFYHCFGCGRHGNAINFLMEHSGVSFV
EAVESLAAHAGMQIPAWKNINSKASDSGETSLNEVRFDEVIQTSSDLYGRMEQAAKFYRVQLKQSELAIAYLKDRGVSGQ
TALHFGIGYAPSGWQNLAEIFTDYPSGDSSHALVRSGLIVAHESKKNYDRFRHRIMFPILDLKKRIIGFGGRALDGSEPK
YLNSPETPLFIKGRELYNLVSAGPAIRKSGRVVVVEGYMDVVMLVQHGIENVVATLGTATTSTHIQKLLRYTDEVVFCFD
GDEAGTKAAWRALETSLPQLKDGKDIKFLFLPDKEDPDSYIRKYGKDAFKNLLEKAQLLSVFFCKELSSRVNLSTSEGRA
RLVQQAEPLLVQINAPIFGFMLMKRISELAGVDQNQLIAFLKPGKKNQPSISRSNVSRPLSVTPYRRLIQILLHTPAYVD
KLDTDLFALNDGQNEEKALLAALVDFLKTSSCTSMDELNSVAIMLHLDQTPHRVLLEKIARDIHVKDVDWDIDAEFTGAM
VRLREAQRKSRVAELHNRPLASLTPEEKKELRRLILS

Sequences:

>Translated_597_residues
MIAQPFIQELLDRIDIVDIVARHLKLKKAGANFTACCPFHNEKTPSFTVNSSKQFYHCFGCGRHGNAINFLMEHSGVSFV
EAVESLAAHAGMQIPAWKNINSKASDSGETSLNEVRFDEVIQTSSDLYGRMEQAAKFYRVQLKQSELAIAYLKDRGVSGQ
TALHFGIGYAPSGWQNLAEIFTDYPSGDSSHALVRSGLIVAHESKKNYDRFRHRIMFPILDLKKRIIGFGGRALDGSEPK
YLNSPETPLFIKGRELYNLVSAGPAIRKSGRVVVVEGYMDVVMLVQHGIENVVATLGTATTSTHIQKLLRYTDEVVFCFD
GDEAGTKAAWRALETSLPQLKDGKDIKFLFLPDKEDPDSYIRKYGKDAFKNLLEKAQLLSVFFCKELSSRVNLSTSEGRA
RLVQQAEPLLVQINAPIFGFMLMKRISELAGVDQNQLIAFLKPGKKNQPSISRSNVSRPLSVTPYRRLIQILLHTPAYVD
KLDTDLFALNDGQNEEKALLAALVDFLKTSSCTSMDELNSVAIMLHLDQTPHRVLLEKIARDIHVKDVDWDIDAEFTGAM
VRLREAQRKSRVAELHNRPLASLTPEEKKELRRLILS
>Mature_597_residues
MIAQPFIQELLDRIDIVDIVARHLKLKKAGANFTACCPFHNEKTPSFTVNSSKQFYHCFGCGRHGNAINFLMEHSGVSFV
EAVESLAAHAGMQIPAWKNINSKASDSGETSLNEVRFDEVIQTSSDLYGRMEQAAKFYRVQLKQSELAIAYLKDRGVSGQ
TALHFGIGYAPSGWQNLAEIFTDYPSGDSSHALVRSGLIVAHESKKNYDRFRHRIMFPILDLKKRIIGFGGRALDGSEPK
YLNSPETPLFIKGRELYNLVSAGPAIRKSGRVVVVEGYMDVVMLVQHGIENVVATLGTATTSTHIQKLLRYTDEVVFCFD
GDEAGTKAAWRALETSLPQLKDGKDIKFLFLPDKEDPDSYIRKYGKDAFKNLLEKAQLLSVFFCKELSSRVNLSTSEGRA
RLVQQAEPLLVQINAPIFGFMLMKRISELAGVDQNQLIAFLKPGKKNQPSISRSNVSRPLSVTPYRRLIQILLHTPAYVD
KLDTDLFALNDGQNEEKALLAALVDFLKTSSCTSMDELNSVAIMLHLDQTPHRVLLEKIARDIHVKDVDWDIDAEFTGAM
VRLREAQRKSRVAELHNRPLASLTPEEKKELRRLILS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=599, Percent_Identity=41.7362270450751, Blast_Score=430, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66898; Mature: 66898

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAQPFIQELLDRIDIVDIVARHLKLKKAGANFTACCPFHNEKTPSFTVNSSKQFYHCFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCCEEEEEC
CGRHGNAINFLMEHSGVSFVEAVESLAAHAGMQIPAWKNINSKASDSGETSLNEVRFDEV
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IQTSSDLYGRMEQAAKFYRVQLKQSELAIAYLKDRGVSGQTALHFGIGYAPSGWQNLAEI
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHH
FTDYPSGDSSHALVRSGLIVAHESKKNYDRFRHRIMFPILDLKKRIIGFGGRALDGSEPK
HHCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLNSPETPLFIKGRELYNLVSAGPAIRKSGRVVVVEGYMDVVMLVQHGIENVVATLGTAT
CCCCCCCCEEEECHHHHHHHHCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCH
TSTHIQKLLRYTDEVVFCFDGDEAGTKAAWRALETSLPQLKDGKDIKFLFLPDKEDPDSY
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHH
IRKYGKDAFKNLLEKAQLLSVFFCKELSSRVNLSTSEGRARLVQQAEPLLVQINAPIFGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCHHHH
MLMKRISELAGVDQNQLIAFLKPGKKNQPSISRSNVSRPLSVTPYRRLIQILLHTPAYVD
HHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCHHHH
KLDTDLFALNDGQNEEKALLAALVDFLKTSSCTSMDELNSVAIMLHLDQTPHRVLLEKIA
HHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCHHHHHHHHHH
RDIHVKDVDWDIDAEFTGAMVRLREAQRKSRVAELHNRPLASLTPEEKKELRRLILS
HHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIAQPFIQELLDRIDIVDIVARHLKLKKAGANFTACCPFHNEKTPSFTVNSSKQFYHCFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCCEEEEEC
CGRHGNAINFLMEHSGVSFVEAVESLAAHAGMQIPAWKNINSKASDSGETSLNEVRFDEV
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IQTSSDLYGRMEQAAKFYRVQLKQSELAIAYLKDRGVSGQTALHFGIGYAPSGWQNLAEI
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHH
FTDYPSGDSSHALVRSGLIVAHESKKNYDRFRHRIMFPILDLKKRIIGFGGRALDGSEPK
HHCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLNSPETPLFIKGRELYNLVSAGPAIRKSGRVVVVEGYMDVVMLVQHGIENVVATLGTAT
CCCCCCCCEEEECHHHHHHHHCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCH
TSTHIQKLLRYTDEVVFCFDGDEAGTKAAWRALETSLPQLKDGKDIKFLFLPDKEDPDSY
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHH
IRKYGKDAFKNLLEKAQLLSVFFCKELSSRVNLSTSEGRARLVQQAEPLLVQINAPIFGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCHHHH
MLMKRISELAGVDQNQLIAFLKPGKKNQPSISRSNVSRPLSVTPYRRLIQILLHTPAYVD
HHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCHHHH
KLDTDLFALNDGQNEEKALLAALVDFLKTSSCTSMDELNSVAIMLHLDQTPHRVLLEKIA
HHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCHHHHHHHHHH
RDIHVKDVDWDIDAEFTGAMVRLREAQRKSRVAELHNRPLASLTPEEKKELRRLILS
HHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]