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The map label for this gene is tolB [H]

Identifier: 114330316

GI number: 114330316

Start: 298209

End: 299480

Strand: Direct

Name: tolB [H]

Synonym: Neut_0289

Alternate gene names: 114330316

Gene position: 298209-299480 (Clockwise)

Preceding gene: 114330315

Following gene: 114330317

Centisome position: 11.21

GC content: 49.21

Gene sequence:

>1272_bases
ATGCGTAATTTTTTGTATTGTACTGGTGTGTTTCTGTTGTTATGGATGAATACACCGCTACAAGCTGCTCTTGATATTGA
AATCTTTGGTGGTGGGGCTAACAGGATGCCGATTGCCGTAGCTCCGTTTAATGGCGAAAAAGGGCTTCCCCAGGATATTT
CGGCGATTGTATCGGCTGATCTGGAGCGCAGCGGGATATTTAAACTGGTTGATCCGTTGGGGTTAACCTCGCAAGAGCCA
GTGAGCTATGTCGATTGGCTGGGGCGAGGTGCGAGTGCGCTGGTCATCGGCAGTACATCAATGTTGCCGGACGGGCGGGT
GGATATCCAGTTTCGCTTGCTGGATGTTGCCAAACAGGTTCAACTGACTGGATTTAGCGGTACCGTTACGATCGAACAGC
TGCGTGCCTTTGCTCATCGTATTGCGGATATTGTGTATGAAGCATTGACAAATGAACCTGGTGTCTTCAGCACGAGGATT
GCCTTCGTCAGAAAACAGGGAAGCCGCTATGCATTGCAAGTGTCAGATTATGATGGGTTCAATGCACGTTCACTGATCGA
GTATACCGAGCCGATCATCTCCCCGGCTTGGTCGCCAGATGGCAGCAAAATTGCTTACACTTCCTTTGAAAAGAAAAAAC
CAGTGGTTTATGTGCAGGCGCTGGCAACCCGTGAACGTAAGGTGGTAGCAAATTTCAAAGGAAGTAACAGTGCCCCGGCC
TGGTCGCCTGATGGTGGAAAATTGGCAGTGGTTTTGACATTGCATGGTGGATCACAGATTTATCTGATCAATGCGGATGG
TAGTGGACTGCAGAGGATTTCACAAAGCCCGGGGATAGATACAGAGCCCAGTTTTTCACCGGATGGAAAATGGCTGATAT
TTACTTCTGACAGAGGAGGAAGTCCGCAGATATATCGTATGCCGGTATCTGGCGGTGCTGCCGAGAGGTTGACCTTTGAA
GGAGATTACAATGTCAGTCCGCGTTATAGCTCGGATGGAAAACGGTTTGTCTATATCCACAGAAATTCTGGCCGATTCAG
CGTTGCTGTACAGGATTTATCGACTCGCCAGATGCAATTACTAACGGATTCCAGTTTTGATGAATCTCCCAGTTTTTCCC
CCAATAGCCGCATGGTTCTGTACACGACGGAAGTGAGTGGGCGTGGTATATTATCCATCGTTTCCAGTGATGGCCAGACG
AGAAGTCGATTTTCGCAGGAAGCGGGTGATGTCCGGGAAGCGGTTTGGGGGCCGTTGCTGAAACAGCGGTAA

Upstream 100 bases:

>100_bases
CGGATCCCGGATTGTTTAATGAGTTTCGTAATTTAAATATTACTGTTTATTATCGTGAATAGTTTTTGACCTACCTCTAC
AACATGGCATGGTGATTCAT

Downstream 100 bases:

>100_bases
TGTGGATAAGAAACGCTCACTGGATGTGGGTTATTTATTTATTTATTTATTTATGGTGAGAAATGAAAAAATTTTTAGCA
GTGCTGGCGATTAGCCTGCT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MRNFLYCTGVFLLLWMNTPLQAALDIEIFGGGANRMPIAVAPFNGEKGLPQDISAIVSADLERSGIFKLVDPLGLTSQEP
VSYVDWLGRGASALVIGSTSMLPDGRVDIQFRLLDVAKQVQLTGFSGTVTIEQLRAFAHRIADIVYEALTNEPGVFSTRI
AFVRKQGSRYALQVSDYDGFNARSLIEYTEPIISPAWSPDGSKIAYTSFEKKKPVVYVQALATRERKVVANFKGSNSAPA
WSPDGGKLAVVLTLHGGSQIYLINADGSGLQRISQSPGIDTEPSFSPDGKWLIFTSDRGGSPQIYRMPVSGGAAERLTFE
GDYNVSPRYSSDGKRFVYIHRNSGRFSVAVQDLSTRQMQLLTDSSFDESPSFSPNSRMVLYTTEVSGRGILSIVSSDGQT
RSRFSQEAGDVREAVWGPLLKQR

Sequences:

>Translated_423_residues
MRNFLYCTGVFLLLWMNTPLQAALDIEIFGGGANRMPIAVAPFNGEKGLPQDISAIVSADLERSGIFKLVDPLGLTSQEP
VSYVDWLGRGASALVIGSTSMLPDGRVDIQFRLLDVAKQVQLTGFSGTVTIEQLRAFAHRIADIVYEALTNEPGVFSTRI
AFVRKQGSRYALQVSDYDGFNARSLIEYTEPIISPAWSPDGSKIAYTSFEKKKPVVYVQALATRERKVVANFKGSNSAPA
WSPDGGKLAVVLTLHGGSQIYLINADGSGLQRISQSPGIDTEPSFSPDGKWLIFTSDRGGSPQIYRMPVSGGAAERLTFE
GDYNVSPRYSSDGKRFVYIHRNSGRFSVAVQDLSTRQMQLLTDSSFDESPSFSPNSRMVLYTTEVSGRGILSIVSSDGQT
RSRFSQEAGDVREAVWGPLLKQR
>Mature_423_residues
MRNFLYCTGVFLLLWMNTPLQAALDIEIFGGGANRMPIAVAPFNGEKGLPQDISAIVSADLERSGIFKLVDPLGLTSQEP
VSYVDWLGRGASALVIGSTSMLPDGRVDIQFRLLDVAKQVQLTGFSGTVTIEQLRAFAHRIADIVYEALTNEPGVFSTRI
AFVRKQGSRYALQVSDYDGFNARSLIEYTEPIISPAWSPDGSKIAYTSFEKKKPVVYVQALATRERKVVANFKGSNSAPA
WSPDGGKLAVVLTLHGGSQIYLINADGSGLQRISQSPGIDTEPSFSPDGKWLIFTSDRGGSPQIYRMPVSGGAAERLTFE
GDYNVSPRYSSDGKRFVYIHRNSGRFSVAVQDLSTRQMQLLTDSSFDESPSFSPNSRMVLYTTEVSGRGILSIVSSDGQT
RSRFSQEAGDVREAVWGPLLKQR

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=431, Percent_Identity=37.8190255220418, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 46239; Mature: 46239

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNFLYCTGVFLLLWMNTPLQAALDIEIFGGGANRMPIAVAPFNGEKGLPQDISAIVSAD
CCCEEHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHC
LERSGIFKLVDPLGLTSQEPVSYVDWLGRGASALVIGSTSMLPDGRVDIQFRLLDVAKQV
CCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHE
QLTGFSGTVTIEQLRAFAHRIADIVYEALTNEPGVFSTRIAFVRKQGSRYALQVSDYDGF
EEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECCCEEEEEEECCCCC
NARSLIEYTEPIISPAWSPDGSKIAYTSFEKKKPVVYVQALATRERKVVANFKGSNSAPA
CHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCC
WSPDGGKLAVVLTLHGGSQIYLINADGSGLQRISQSPGIDTEPSFSPDGKWLIFTSDRGG
CCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
SPQIYRMPVSGGAAERLTFEGDYNVSPRYSSDGKRFVYIHRNSGRFSVAVQDLSTRQMQL
CCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCEEEE
LTDSSFDESPSFSPNSRMVLYTTEVSGRGILSIVSSDGQTRSRFSQEAGDVREAVWGPLL
EECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KQR
HCC
>Mature Secondary Structure
MRNFLYCTGVFLLLWMNTPLQAALDIEIFGGGANRMPIAVAPFNGEKGLPQDISAIVSAD
CCCEEHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHC
LERSGIFKLVDPLGLTSQEPVSYVDWLGRGASALVIGSTSMLPDGRVDIQFRLLDVAKQV
CCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHE
QLTGFSGTVTIEQLRAFAHRIADIVYEALTNEPGVFSTRIAFVRKQGSRYALQVSDYDGF
EEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECCCEEEEEEECCCCC
NARSLIEYTEPIISPAWSPDGSKIAYTSFEKKKPVVYVQALATRERKVVANFKGSNSAPA
CHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCC
WSPDGGKLAVVLTLHGGSQIYLINADGSGLQRISQSPGIDTEPSFSPDGKWLIFTSDRGG
CCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
SPQIYRMPVSGGAAERLTFEGDYNVSPRYSSDGKRFVYIHRNSGRFSVAVQDLSTRQMQL
CCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCEEEE
LTDSSFDESPSFSPNSRMVLYTTEVSGRGILSIVSSDGQTRSRFSQEAGDVREAVWGPLL
EECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KQR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]