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The map label for this gene is mraW

Identifier: 114330281

GI number: 114330281

Start: 268841

End: 269794

Strand: Reverse

Name: mraW

Synonym: Neut_0254

Alternate gene names: 114330281

Gene position: 269794-268841 (Counterclockwise)

Preceding gene: 114330282

Following gene: 114330280

Centisome position: 10.14

GC content: 51.15

Gene sequence:

>954_bases
GTGCACATATCCGTTCTGCTGGAAGAAGCTGTAGATGCGCTAAATATTCAAAAAGGCGGCATCTATGTGGATGGAACGTA
TGGCCGGGGCGGCCACAGCCGCCTGATTCTGTCGCGACTGGACAAGTCAGGCCAGTTAATCGCGTTTGACAAGGATCCGG
CGGCTATTTCCGAAGCTCGATCCATCCTGGATGAAAGATTTCAGGCAGTACACAGCAGTTATGCCGGAATGTACACAGCA
CTTCAGTCGCTGGGCATAAACCGGGTTGACGGTATTCTGCTGGATCTTGGTGTATCGTCAATTCAACTGGACGAAGCTTC
GCGCGGATTCAGCTTTCGTCATGATGGTCCCCTGGATATGCGCATGGATTCCAGCCGGGGTAAAACAGCAGCAGAGTGGC
TGACTATGGCGTCAGAAACAGAATTAAAGGAAATAATCAGAACCTACGGCGAAGAGCGGTACGCAGGACAGATTGCAAGT
GCAATTGTTATGGAGCAGGCACGCCAGCCCATCAGTACCACGCTTCGGCTTGCCGAGATTGTCGCAGCAGTTGTGCGTAA
GCGCGGTCACCGGGATGATCGACAGCATCCAGCGACACGTACTTTCCAGGCGATACGGATTCACCTTAACCAGGAGCTTG
AAGAACTATCAATGACATTGCCACAATGTGTGGAGTTGCTGAATACAGGCGGCCGGTTGGTGGTGATCAGCTTTCATTCA
TTAGAAGATCGCATTGTCAAGCGTTTCATGCGTATGCAGACCGGTACCGATACTCTGCCGCGAAGATTGCCTATCAGGGA
GGAGGAAAGCCGTTTACACAATCAGCAAAAATTAAGAATTATCGGTAAAAAAATTCGCCCAGGATCGGATGAAGTATCCG
CCAATCCACGGGCACGCAGCGCTGTCATGCGGGTGGCAGAAAAACTGGAAACCAGAAACGCGATAAGCAGATGA

Upstream 100 bases:

>100_bases
GGCGAAATGGGATCTGGAAATCGATACGGCAACTGCTTGCAAGGATGGAGATATTCCTCCCGAACTGGAAGGATTTTCAC
TGTAATGTGCCGGTAACGCC

Downstream 100 bases:

>100_bases
TCAAGCTAAATATTTTTCTATTTGTAATATTGATTATCTGTGGATTGGGTATTGTGACCGCACGGTATGAAGCACGTAAA
CTTTTCATGGAACAGGAAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MHISVLLEEAVDALNIQKGGIYVDGTYGRGGHSRLILSRLDKSGQLIAFDKDPAAISEARSILDERFQAVHSSYAGMYTA
LQSLGINRVDGILLDLGVSSIQLDEASRGFSFRHDGPLDMRMDSSRGKTAAEWLTMASETELKEIIRTYGEERYAGQIAS
AIVMEQARQPISTTLRLAEIVAAVVRKRGHRDDRQHPATRTFQAIRIHLNQELEELSMTLPQCVELLNTGGRLVVISFHS
LEDRIVKRFMRMQTGTDTLPRRLPIREEESRLHNQQKLRIIGKKIRPGSDEVSANPRARSAVMRVAEKLETRNAISR

Sequences:

>Translated_317_residues
MHISVLLEEAVDALNIQKGGIYVDGTYGRGGHSRLILSRLDKSGQLIAFDKDPAAISEARSILDERFQAVHSSYAGMYTA
LQSLGINRVDGILLDLGVSSIQLDEASRGFSFRHDGPLDMRMDSSRGKTAAEWLTMASETELKEIIRTYGEERYAGQIAS
AIVMEQARQPISTTLRLAEIVAAVVRKRGHRDDRQHPATRTFQAIRIHLNQELEELSMTLPQCVELLNTGGRLVVISFHS
LEDRIVKRFMRMQTGTDTLPRRLPIREEESRLHNQQKLRIIGKKIRPGSDEVSANPRARSAVMRVAEKLETRNAISR
>Mature_317_residues
MHISVLLEEAVDALNIQKGGIYVDGTYGRGGHSRLILSRLDKSGQLIAFDKDPAAISEARSILDERFQAVHSSYAGMYTA
LQSLGINRVDGILLDLGVSSIQLDEASRGFSFRHDGPLDMRMDSSRGKTAAEWLTMASETELKEIIRTYGEERYAGQIAS
AIVMEQARQPISTTLRLAEIVAAVVRKRGHRDDRQHPATRTFQAIRIHLNQELEELSMTLPQCVELLNTGGRLVVISFHS
LEDRIVKRFMRMQTGTDTLPRRLPIREEESRLHNQQKLRIIGKKIRPGSDEVSANPRARSAVMRVAEKLETRNAISR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=39.7058823529412, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=42.1052631578947, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1786270, Length=309, Percent_Identity=54.3689320388349, Blast_Score=331, Evalue=5e-92,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=39.6396396396396, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=39.6396396396396, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_NITEC (Q0AJD3)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746503.1
- ProteinModelPortal:   Q0AJD3
- STRING:   Q0AJD3
- GeneID:   4271993
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0254
- NMPDR:   fig|335283.3.peg.2127
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q0AJD3
- ProtClustDB:   PRK00050
- BioCyc:   NEUT335283:NEUT_0254-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35581; Mature: 35581

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 78-78 BINDING 95-95 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISVLLEEAVDALNIQKGGIYVDGTYGRGGHSRLILSRLDKSGQLIAFDKDPAAISEAR
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHH
SILDERFQAVHSSYAGMYTALQSLGINRVDGILLDLGVSSIQLDEASRGFSFRHDGPLDM
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEECHHHCCCCCCCCCCCCE
RMDSSRGKTAAEWLTMASETELKEIIRTYGEERYAGQIASAIVMEQARQPISTTLRLAEI
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VAAVVRKRGHRDDRQHPATRTFQAIRIHLNQELEELSMTLPQCVELLNTGGRLVVISFHS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHH
LEDRIVKRFMRMQTGTDTLPRRLPIREEESRLHNQQKLRIIGKKIRPGSDEVSANPRARS
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
AVMRVAEKLETRNAISR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHISVLLEEAVDALNIQKGGIYVDGTYGRGGHSRLILSRLDKSGQLIAFDKDPAAISEAR
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHH
SILDERFQAVHSSYAGMYTALQSLGINRVDGILLDLGVSSIQLDEASRGFSFRHDGPLDM
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEECHHHCCCCCCCCCCCCE
RMDSSRGKTAAEWLTMASETELKEIIRTYGEERYAGQIASAIVMEQARQPISTTLRLAEI
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VAAVVRKRGHRDDRQHPATRTFQAIRIHLNQELEELSMTLPQCVELLNTGGRLVVISFHS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHH
LEDRIVKRFMRMQTGTDTLPRRLPIREEESRLHNQQKLRIIGKKIRPGSDEVSANPRARS
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
AVMRVAEKLETRNAISR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA