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The map label for this gene is cysS

Identifier: 114330220

GI number: 114330220

Start: 191382

End: 192785

Strand: Reverse

Name: cysS

Synonym: Neut_0191

Alternate gene names: 114330220

Gene position: 192785-191382 (Counterclockwise)

Preceding gene: 114330224

Following gene: 114330216

Centisome position: 7.24

GC content: 51.99

Gene sequence:

>1404_bases
ATGCTCAAAATTTACGATACGCTCACCCGCTCCAGACGCGAATTTATCCCTCTTACACCCGGGAAAGTCAAAATGTATGT
GTGTGGCATGACTGTTTATGATCACTGCCATCTTGGACACGCTCGTGTATTGGTCGTATTTGACAGCGTAGTACGCTGGC
TACAGACTCTTGACTACGAGGTGACTTATGTCCGCAATATCACTGACATCGACGACAAGATTATTCGCCGCGCGCTGGAA
AACCATGAGCCGTTTGGAGCACTGACTGCCCGCTACATTCAAGCAATGGAAGAAGACGCTGCGGCATTGGGCATCATCAG
TCCTTCTTTCGAGCCGCGCGCAACTGAATGCATCGAGAGTATGGTCACAATGATCGAATCATTACTGAGCAAAGGGCTCG
CCTACGTAGCAAACAACGGCGATGTATTTTACGACGTACGCCGTTTCCCCGGCTATGGCAAGCTCTCGGGTAAATCACTC
GATGATTTGCGTGCCGGTGAGCGCGTGGAGATCGATACCAACAAGCGGGATCCCTTGGATTTTGTGTTATGGAAAGCTGC
CAAACCCGATGAACCCAGCTGGGATTCGCCGTGGGGCAAGGGACGTCCGGGCTGGCATATTGAATGCTCCGCCATGAGCG
GGCACTACCTGGGTGAGCGGTTTGACATCCATGGGGGCGGTCAGGATTTACAATTCCCGCATCACGAGAACGAAATCGCG
CAAAGTGAAGGCGTCCACGGCCACCCCCATGTCAATTACTGGATGCACAACGGATTTGTGCGCGTAGACAATGAAAAAAT
GTCCAAATCGCTGGGTAATTTTTTCACTGTGCGGGAAGTGCTGGCACGCTACCAGCCCGAGGTAGTTCGTTTTTTCATTG
TGCGGGCACACTATCGCAGCCCGCTCAATTATTCTGATGCCCACCTCAACGATGCCAGAAACGCACTGGAACGTCTCTAC
ACAGCGCTGAAGAATCACGCGCCATCACAGGCACAGGCAGCTGAAGAAGCAACTGCTACGATTGACTGGGTAAATGATGG
CTACGCTCAGCGTTTCATGGCGGCGATGAATGATGATTTCAACACACCAGAGGCGGTTGCGGTGCTGTTCGATCTGGCAA
GCGAAGCAAACCGGACGGGTAATTCACACTATGCCAGTTTGCTCAAGGCTCTAGGTGGAATACTGGGTCTGTTACAGCAA
TCACCAAGGCAATATTTGCAACATCCCGCACACCCACAGAATGACCACCATTCCATAGAAGAAATTGAACGCATGATCCA
GCAGCGATTACAGGCGCGCAAGGAGAAGAATTTTGCGCAGGCTGATGCACTGCGTCAGCAACTGGCCGAAGCCGGCATTA
TTCTGGAAGACGGTCCGCAAGGAACAACCTGGCGACGACAATAA

Upstream 100 bases:

>100_bases
TGCCCCTGGTTCTGCCGGGCGGACGGTTATAAAGAAAAGTCCCTGATTTTATCAATAAAATAAGCACTTCTCACAACTAT
CTCTCTTGCATACGCCTCCG

Downstream 100 bases:

>100_bases
CGCAGAATTTCATCAAGGTATTTGCCAGATATAAAGTGCGGCACCACTAAAATCATCCAGCTGGATACACCGATCAGGCA
CGACGCCTGGAACAGTAAAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLKIYDTLTRSRREFIPLTPGKVKMYVCGMTVYDHCHLGHARVLVVFDSVVRWLQTLDYEVTYVRNITDIDDKIIRRALE
NHEPFGALTARYIQAMEEDAAALGIISPSFEPRATECIESMVTMIESLLSKGLAYVANNGDVFYDVRRFPGYGKLSGKSL
DDLRAGERVEIDTNKRDPLDFVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMSGHYLGERFDIHGGGQDLQFPHHENEIA
QSEGVHGHPHVNYWMHNGFVRVDNEKMSKSLGNFFTVREVLARYQPEVVRFFIVRAHYRSPLNYSDAHLNDARNALERLY
TALKNHAPSQAQAAEEATATIDWVNDGYAQRFMAAMNDDFNTPEAVAVLFDLASEANRTGNSHYASLLKALGGILGLLQQ
SPRQYLQHPAHPQNDHHSIEEIERMIQQRLQARKEKNFAQADALRQQLAEAGIILEDGPQGTTWRRQ

Sequences:

>Translated_467_residues
MLKIYDTLTRSRREFIPLTPGKVKMYVCGMTVYDHCHLGHARVLVVFDSVVRWLQTLDYEVTYVRNITDIDDKIIRRALE
NHEPFGALTARYIQAMEEDAAALGIISPSFEPRATECIESMVTMIESLLSKGLAYVANNGDVFYDVRRFPGYGKLSGKSL
DDLRAGERVEIDTNKRDPLDFVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMSGHYLGERFDIHGGGQDLQFPHHENEIA
QSEGVHGHPHVNYWMHNGFVRVDNEKMSKSLGNFFTVREVLARYQPEVVRFFIVRAHYRSPLNYSDAHLNDARNALERLY
TALKNHAPSQAQAAEEATATIDWVNDGYAQRFMAAMNDDFNTPEAVAVLFDLASEANRTGNSHYASLLKALGGILGLLQQ
SPRQYLQHPAHPQNDHHSIEEIERMIQQRLQARKEKNFAQADALRQQLAEAGIILEDGPQGTTWRRQ
>Mature_467_residues
MLKIYDTLTRSRREFIPLTPGKVKMYVCGMTVYDHCHLGHARVLVVFDSVVRWLQTLDYEVTYVRNITDIDDKIIRRALE
NHEPFGALTARYIQAMEEDAAALGIISPSFEPRATECIESMVTMIESLLSKGLAYVANNGDVFYDVRRFPGYGKLSGKSL
DDLRAGERVEIDTNKRDPLDFVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMSGHYLGERFDIHGGGQDLQFPHHENEIA
QSEGVHGHPHVNYWMHNGFVRVDNEKMSKSLGNFFTVREVLARYQPEVVRFFIVRAHYRSPLNYSDAHLNDARNALERLY
TALKNHAPSQAQAAEEATATIDWVNDGYAQRFMAAMNDDFNTPEAVAVLFDLASEANRTGNSHYASLLKALGGILGLLQQ
SPRQYLQHPAHPQNDHHSIEEIERMIQQRLQARKEKNFAQADALRQQLAEAGIILEDGPQGTTWRRQ

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=481, Percent_Identity=37.2141372141372, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI10835051, Length=260, Percent_Identity=37.6923076923077, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI62240992, Length=260, Percent_Identity=37.6923076923077, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI303304987, Length=260, Percent_Identity=37.6923076923077, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI21269877, Length=260, Percent_Identity=37.6923076923077, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1786737, Length=473, Percent_Identity=57.2938689217759, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17509797, Length=255, Percent_Identity=38.0392156862745, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17509795, Length=255, Percent_Identity=38.0392156862745, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324082, Length=386, Percent_Identity=31.6062176165803, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI19922218, Length=509, Percent_Identity=33.2023575638507, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19922364, Length=350, Percent_Identity=34.2857142857143, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_NITEC (Q0AJJ4)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746442.1
- ProteinModelPortal:   Q0AJJ4
- SMR:   Q0AJJ4
- STRING:   Q0AJJ4
- GeneID:   4272432
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0191
- NMPDR:   fig|335283.3.peg.1689
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q0AJJ4
- ProtClustDB:   PRK00260
- BioCyc:   NEUT335283:NEUT_0191-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53101; Mature: 53101

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIYDTLTRSRREFIPLTPGKVKMYVCGMTVYDHCHLGHARVLVVFDSVVRWLQTLDYE
CCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
VTYVRNITDIDDKIIRRALENHEPFGALTARYIQAMEEDAAALGIISPSFEPRATECIES
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
MVTMIESLLSKGLAYVANNGDVFYDVRRFPGYGKLSGKSLDDLRAGERVEIDTNKRDPLD
HHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCHH
FVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMSGHYLGERFDIHGGGQDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHH
QSEGVHGHPHVNYWMHNGFVRVDNEKMSKSLGNFFTVREVLARYQPEVVRFFIVRAHYRS
HHCCCCCCCCEEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PLNYSDAHLNDARNALERLYTALKNHAPSQAQAAEEATATIDWVNDGYAQRFMAAMNDDF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEECCCHHHHHHHHHHCCCC
NTPEAVAVLFDLASEANRTGNSHYASLLKALGGILGLLQQSPRQYLQHPAHPQNDHHSIE
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHHH
EIERMIQQRLQARKEKNFAQADALRQQLAEAGIILEDGPQGTTWRRQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIYDTLTRSRREFIPLTPGKVKMYVCGMTVYDHCHLGHARVLVVFDSVVRWLQTLDYE
CCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
VTYVRNITDIDDKIIRRALENHEPFGALTARYIQAMEEDAAALGIISPSFEPRATECIES
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
MVTMIESLLSKGLAYVANNGDVFYDVRRFPGYGKLSGKSLDDLRAGERVEIDTNKRDPLD
HHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCHH
FVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMSGHYLGERFDIHGGGQDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHH
QSEGVHGHPHVNYWMHNGFVRVDNEKMSKSLGNFFTVREVLARYQPEVVRFFIVRAHYRS
HHCCCCCCCCEEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PLNYSDAHLNDARNALERLYTALKNHAPSQAQAAEEATATIDWVNDGYAQRFMAAMNDDF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEECCCHHHHHHHHHHCCCC
NTPEAVAVLFDLASEANRTGNSHYASLLKALGGILGLLQQSPRQYLQHPAHPQNDHHSIE
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHHH
EIERMIQQRLQARKEKNFAQADALRQQLAEAGIILEDGPQGTTWRRQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA