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The map label for this gene is nodQ [H]

Identifier: 114330195

GI number: 114330195

Start: 165948

End: 167645

Strand: Reverse

Name: nodQ [H]

Synonym: Neut_0164

Alternate gene names: 114330195

Gene position: 167645-165948 (Counterclockwise)

Preceding gene: 114330198

Following gene: 114330182

Centisome position: 6.3

GC content: 47.17

Gene sequence:

>1698_bases
TTGAGTACTCTCTCCAAAGCAGCAGACCACCAACCAGCACAAGACAACAATTTCAACTTCAAACACAAATCTGGCGCAGT
CGATAGTGAAACCTGGTCCATTTTTTCAACGACAGGACGTGAATTCTCGATCACCATTGCAACAGCACCCGAGAGAAACA
ACCGCGAAATACATGCTGCACTCTTCAAGGCAAACGTCGCAATCATTCTGATCGATGCCGCCTGCGGAATACAAACCCAG
GCCAAATGGTACGCTTATCTGGCATCATCCTTCAAAATTCGCCATCTCATTCTCGGCGTCAACAAGATGGATCAGGTGGA
TTTTGATCCCCATATCTTTCACGGTATCGAACAGGACTTTCACGATCTGTGCAGTCACCTGGATTTTGCAAGCACCGCAA
TCATACCCACGGTGTCGCTTCAGGGGAATAACGTTCTCAATCCCTCCGTCCATACCCCCTGGTATGACGGCCTCTCACTG
ATTGACTATCTGACAACCATAGAAACAGCAAGAATAACTGACAACCGCTTCATATTTCTGGTGCAAGAAAACAATCCAGC
GAACATCCGCTCTGATCGTTGCAACGGAATGATCGCTAATGGTCAAATTACTATAGGTGACGTGGTACGTATTGCTGCCT
CCGGAGAAATCGCGCAGGTAAAAAATATTCGCACTTTAAATAGCGATGTATTTGAAGCGCGTACGAATGAAGTTATCACT
CTGGCATTGGATAAAGAGGTGAAAATTTCGTGCAATGATGTTATTTCAGCCGCGCATTCACCTCTGGAAACAACGGATCA
ATTTACTGCCGTAGTAATCTGGACGCATGCTGAACCAGGATTAGTTAGTCGAAACTACCAGCTAATCCTGGCGAATCAGC
AAGCCGATGCATCCATTACTACCATCAAATATCGCCTTGATATAAATACTGGCGCCCACGAAGCATGCCAACAGCTAAAA
CAAAATGATATTACGGTATGCAATCTGGCGCTGAACCATCCGGTAGCATTCGATACCTATACCACATTGCCCGTACTGGG
CAATTTTGTGTTGGTGGATCAATACAGCCACGCTACGATAGCCACGGGATTGATTATGCATAATCTGCGCCGGGCACAAA
ATGTGCACCGGCAGGCCCTCAGCATTTGTCGTGAAGATCGTGAACGTTTAAATGGTCACAAAGGTAAGGTCATCTGGCTC
ACCGGATTATCCGGCTCCGGCAAATCCACCATTGCCAACGCACTTGAAAAGGAGCTGCACTCACAAGGCAAACGAACCTA
TATCCTGGATGGCGACAATATGCGCCACGGATTAAACAAAGACCTTGGTTTTACCAATGCCGACCGGGTAGAAAACATCC
GCCGTGTTGCAGAAGTAGCAAAACTGATGATGGATGCCGGCCTGATCGTGATCACCGCTTTCATCAGTCCATTCAGAGCG
GAACGCGAAATGGCCCGACAACTTATCGGGAAAGAAAACTTTGTCGAGGTTTATATCAGTACACCGCTAGAGGTATGCGA
ACAGCGCGACCCCAAAGGATTGTACAAAAAAGCACGGAGTGGACAGCTACCTAACATGACAGGGATTAATAGCCCTTACG
AGCCACCAGAACACCCCAGCCTGGTCATCAATGGTACAAACCAGAACATTTCCGCAATACTAAGGCCGTTACTTCAACTT
GTCAGCAGCCTTCAATAA

Upstream 100 bases:

>100_bases
AACAACCAAACCGCTAATAACATCCACTCAATCTGACAGACTGCACGTTCTCATTTGCGTAGATAAAAGTAGCGGTAAGC
GTGCCCTATTCGACTTCCTG

Downstream 100 bases:

>100_bases
CATGTCAGAGAAATTATGATGATCTTTACAGACCAGCTTTCAGCAACCGGGGATTTGTTGTCAGTACTTCAATACCTGCA
TGTTGAAAATCAGCGTCATT

Product: sulfate adenylyltransferase/adenylyl-sulfate kinase

Products: NA

Alternate protein names: Nodulation protein Q; Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSTLSKAADHQPAQDNNFNFKHKSGAVDSETWSIFSTTGREFSITIATAPERNNREIHAALFKANVAIILIDAACGIQTQ
AKWYAYLASSFKIRHLILGVNKMDQVDFDPHIFHGIEQDFHDLCSHLDFASTAIIPTVSLQGNNVLNPSVHTPWYDGLSL
IDYLTTIETARITDNRFIFLVQENNPANIRSDRCNGMIANGQITIGDVVRIAASGEIAQVKNIRTLNSDVFEARTNEVIT
LALDKEVKISCNDVISAAHSPLETTDQFTAVVIWTHAEPGLVSRNYQLILANQQADASITTIKYRLDINTGAHEACQQLK
QNDITVCNLALNHPVAFDTYTTLPVLGNFVLVDQYSHATIATGLIMHNLRRAQNVHRQALSICREDRERLNGHKGKVIWL
TGLSGSGKSTIANALEKELHSQGKRTYILDGDNMRHGLNKDLGFTNADRVENIRRVAEVAKLMMDAGLIVITAFISPFRA
EREMARQLIGKENFVEVYISTPLEVCEQRDPKGLYKKARSGQLPNMTGINSPYEPPEHPSLVINGTNQNISAILRPLLQL
VSSLQ

Sequences:

>Translated_565_residues
MSTLSKAADHQPAQDNNFNFKHKSGAVDSETWSIFSTTGREFSITIATAPERNNREIHAALFKANVAIILIDAACGIQTQ
AKWYAYLASSFKIRHLILGVNKMDQVDFDPHIFHGIEQDFHDLCSHLDFASTAIIPTVSLQGNNVLNPSVHTPWYDGLSL
IDYLTTIETARITDNRFIFLVQENNPANIRSDRCNGMIANGQITIGDVVRIAASGEIAQVKNIRTLNSDVFEARTNEVIT
LALDKEVKISCNDVISAAHSPLETTDQFTAVVIWTHAEPGLVSRNYQLILANQQADASITTIKYRLDINTGAHEACQQLK
QNDITVCNLALNHPVAFDTYTTLPVLGNFVLVDQYSHATIATGLIMHNLRRAQNVHRQALSICREDRERLNGHKGKVIWL
TGLSGSGKSTIANALEKELHSQGKRTYILDGDNMRHGLNKDLGFTNADRVENIRRVAEVAKLMMDAGLIVITAFISPFRA
EREMARQLIGKENFVEVYISTPLEVCEQRDPKGLYKKARSGQLPNMTGINSPYEPPEHPSLVINGTNQNISAILRPLLQL
VSSLQ
>Mature_564_residues
STLSKAADHQPAQDNNFNFKHKSGAVDSETWSIFSTTGREFSITIATAPERNNREIHAALFKANVAIILIDAACGIQTQA
KWYAYLASSFKIRHLILGVNKMDQVDFDPHIFHGIEQDFHDLCSHLDFASTAIIPTVSLQGNNVLNPSVHTPWYDGLSLI
DYLTTIETARITDNRFIFLVQENNPANIRSDRCNGMIANGQITIGDVVRIAASGEIAQVKNIRTLNSDVFEARTNEVITL
ALDKEVKISCNDVISAAHSPLETTDQFTAVVIWTHAEPGLVSRNYQLILANQQADASITTIKYRLDINTGAHEACQQLKQ
NDITVCNLALNHPVAFDTYTTLPVLGNFVLVDQYSHATIATGLIMHNLRRAQNVHRQALSICREDRERLNGHKGKVIWLT
GLSGSGKSTIANALEKELHSQGKRTYILDGDNMRHGLNKDLGFTNADRVENIRRVAEVAKLMMDAGLIVITAFISPFRAE
REMARQLIGKENFVEVYISTPLEVCEQRDPKGLYKKARSGQLPNMTGINSPYEPPEHPSLVINGTNQNISAILRPLLQLV
SSLQ

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG0529

COG function: function code P; Adenylylsulfate kinase and related kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI46094058, Length=202, Percent_Identity=48.019801980198, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI34447231, Length=204, Percent_Identity=48.5294117647059, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI62912492, Length=204, Percent_Identity=48.5294117647059, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI4503475, Length=377, Percent_Identity=23.342175066313, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI223555963, Length=323, Percent_Identity=24.4582043343653, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI5729864, Length=323, Percent_Identity=24.4582043343653, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789107, Length=189, Percent_Identity=57.1428571428571, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1789108, Length=343, Percent_Identity=29.4460641399417, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17542422, Length=199, Percent_Identity=50.7537688442211, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6322852, Length=160, Percent_Identity=56.875, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6325337, Length=373, Percent_Identity=22.2520107238606, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319594, Length=373, Percent_Identity=22.2520107238606, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24667040, Length=200, Percent_Identity=50, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI116007838, Length=211, Percent_Identity=48.8151658767773, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24667044, Length=211, Percent_Identity=48.8151658767773, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24667032, Length=200, Percent_Identity=50, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24667028, Length=200, Percent_Identity=50, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24667036, Length=200, Percent_Identity=50, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24652838, Length=373, Percent_Identity=23.8605898123324, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137572, Length=373, Percent_Identity=23.8605898123324, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45553807, Length=373, Percent_Identity=23.3243967828418, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45553816, Length=373, Percent_Identity=23.3243967828418, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651721, Length=373, Percent_Identity=23.3243967828418, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17864154, Length=373, Percent_Identity=23.3243967828418, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 62711; Mature: 62580

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLSKAADHQPAQDNNFNFKHKSGAVDSETWSIFSTTGREFSITIATAPERNNREIHAA
CCCHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCEEEE
LFKANVAIILIDAACGIQTQAKWYAYLASSFKIRHLILGVNKMDQVDFDPHIFHGIEQDF
EEECCEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
HDLCSHLDFASTAIIPTVSLQGNNVLNPSVHTPWYDGLSLIDYLTTIETARITDNRFIFL
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCEEEEE
VQENNPANIRSDRCNGMIANGQITIGDVVRIAASGEIAQVKNIRTLNSDVFEARTNEVIT
EECCCCCCCCCCCCCCEEECCEEEHHHEEEEECCCCHHHHHHHHHCCCHHHHCCCCCEEE
LALDKEVKISCNDVISAAHSPLETTDQFTAVVIWTHAEPGLVSRNYQLILANQQADASIT
EEECCCEEEEHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEECCCEEEEEECCCCCCEEE
TIKYRLDINTGAHEACQQLKQNDITVCNLALNHPVAFDTYTTLPVLGNFVLVDQYSHATI
EEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCEEECCCCHHHHHCCEEEEECCCCHHH
ATGLIMHNLRRAQNVHRQALSICREDRERLNGHKGKVIWLTGLSGSGKSTIANALEKELH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
SQGKRTYILDGDNMRHGLNKDLGFTNADRVENIRRVAEVAKLMMDAGLIVITAFISPFRA
HCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EREMARQLIGKENFVEVYISTPLEVCEQRDPKGLYKKARSGQLPNMTGINSPYEPPEHPS
HHHHHHHHHCCCCEEEEEECCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
LVINGTNQNISAILRPLLQLVSSLQ
EEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLSKAADHQPAQDNNFNFKHKSGAVDSETWSIFSTTGREFSITIATAPERNNREIHAA
CCHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCEEEE
LFKANVAIILIDAACGIQTQAKWYAYLASSFKIRHLILGVNKMDQVDFDPHIFHGIEQDF
EEECCEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
HDLCSHLDFASTAIIPTVSLQGNNVLNPSVHTPWYDGLSLIDYLTTIETARITDNRFIFL
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCEEEEE
VQENNPANIRSDRCNGMIANGQITIGDVVRIAASGEIAQVKNIRTLNSDVFEARTNEVIT
EECCCCCCCCCCCCCCEEECCEEEHHHEEEEECCCCHHHHHHHHHCCCHHHHCCCCCEEE
LALDKEVKISCNDVISAAHSPLETTDQFTAVVIWTHAEPGLVSRNYQLILANQQADASIT
EEECCCEEEEHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEECCCEEEEEECCCCCCEEE
TIKYRLDINTGAHEACQQLKQNDITVCNLALNHPVAFDTYTTLPVLGNFVLVDQYSHATI
EEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCEEECCCCHHHHHCCEEEEECCCCHHH
ATGLIMHNLRRAQNVHRQALSICREDRERLNGHKGKVIWLTGLSGSGKSTIANALEKELH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
SQGKRTYILDGDNMRHGLNKDLGFTNADRVENIRRVAEVAKLMMDAGLIVITAFISPFRA
HCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EREMARQLIGKENFVEVYISTPLEVCEQRDPKGLYKKARSGQLPNMTGINSPYEPPEHPS
HHHHHHHHHCCCCEEEEEECCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
LVINGTNQNISAILRPLLQLVSSLQ
EEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9421916 [H]