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The map label for this gene is yfhA [H]

Identifier: 113970040

GI number: 113970040

Start: 2011679

End: 2013223

Strand: Direct

Name: yfhA [H]

Synonym: Shewmr4_1701

Alternate gene names: 113970040

Gene position: 2011679-2013223 (Clockwise)

Preceding gene: 113970039

Following gene: 113970041

Centisome position: 42.74

GC content: 40.58

Gene sequence:

>1545_bases
ATGACATGTTTGCTTTTACTATTGATAAATTTAAATATTGGTTTAGGGATGAACATAATTAACGAAAAAGTGTTGGTTAG
TTGGATTGGTGGCAACGATTTAGAATCAAAGGCGAGTAAAATGACTGGCCCTATATGGGCGACAGTATCTGATGTTACCT
TTGATAGGCTTGAGCTTATTTACAATTATCCGTCGGATCTAGTTACTCCATACCTTGAACTACTCACTGGCAATCTGAGT
TGTCCAGTCAACGCACGTAAGGCCGATTTATCCTCGCCAATTAATTTTGGAGAAATTTACCTCGCTGCAGAACGGCTACT
GGACGTTATTAGTTGTGAAACCATTGCACTGTCTATTTTATTGAGTCCAGGTACACCTGCTATGCAGGCTGTATGGATAT
TACTCGGTAAAACTCGATTCGCCTGCAACTTTTATCAAGCATCAAAAGAACAAGGCGTTAATATTGTAGAAATTCCTTTT
AAGCTCACTGCTGAATATATTCCTTCGATGACTAATCTAGCCCCTACTGAATTTGGGCAACTTGGTCTATTGGACTCTGA
TGCTGATCCTGCCTTTGATGACATTATTACCCTTGATCCTGAAATGTTACTACTTAAAGCCAAGGCACAAGTTTTAGCAT
CGCATGAAGTATCTGTGCTGATTTGTGGTGAGAGTGGTACCGGCAAAGAGATGTTTGCTCGGGCAATTCATAATGCAAGT
GCGAGAAGGGATAAACCATTTGTTGCGGTCAATTGTGGCGCATTTCCCTCAGAACTTATTGATTCAATACTTTTTGGTCA
TAAAAAGGGGGCTTTTACTGGTGCGGTTTCAGATAAGGTAGGAGTTTTTGAATTGGCACATTCAGGTACTCTTTTTTTGG
ATGAGTTTGGCGAGCTTGATAGTTCAGCCCAAGTCAGGCTACTTAGAGTATTACAAGATGGTAAATTCACTCGCTTGGGA
GATAGTAAAGAACGTAGCAGCAATTTTCGTTTGATCACAGCAACTAATCGAGACTTGATGGCTGACGTTTCCAAGGGTAG
GTTCAGGGAGGATTTATTCTATAGGGTTGCCATTGGTGTGTTGTCCTTACCACCTCTAAGGTCTCGACAAAGCGATTTAG
ACCATCTAGCAGATCAGTTTACCGCCATGCTCACACAAGAATACCCTTCACTAGGTGGTAAAAAAATTTCCACTGCTGCA
AAAAAAATTATCAGTAATCATCGTTGGCCTGGAAATATCAGAGAGCTGAAAGCAACGATTCTTAGGGCAGCCTTATGGTC
TGAAACTGCCGTATTGGAAGACGTTGATATACTCAGGGCGATACTTTCGACGCTCCAAAATAGTGAGAGCATTCTCGAAA
GAGATATATCAAAAGGTGTTGATATAAAAAGTATTATTGATCTAGTAGAGCGGCACTATTTAGAACGAGGATTGGCATTT
ACGTCAGGGAATAAACGTAAAACGGCGTTGTTACTCGGCTACAACAATCACCAAACTTTAAATAATCGCCTTAAAAAACT
CGGCCTAGAAAACGACAATGACTAA

Upstream 100 bases:

>100_bases
ACAAAGAGCTGTAATAGGCAATATTGATTCAAATGCATTGAAAAATGGTTGTTTTTCTGTAAATTATCAAACGCTACAAG
TCTGACGAACAACAGCCAAC

Downstream 100 bases:

>100_bases
ATTGGCATAAAAGTCGCTACATGGGTTTGACCCTGAATTAAGCCAGGGATTGCAATTCAACATAAGGAGCTCCACTGTGA
CACAATTAACGGCAATACAA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTCLLLLLINLNIGLGMNIINEKVLVSWIGGNDLESKASKMTGPIWATVSDVTFDRLELIYNYPSDLVTPYLELLTGNLS
CPVNARKADLSSPINFGEIYLAAERLLDVISCETIALSILLSPGTPAMQAVWILLGKTRFACNFYQASKEQGVNIVEIPF
KLTAEYIPSMTNLAPTEFGQLGLLDSDADPAFDDIITLDPEMLLLKAKAQVLASHEVSVLICGESGTGKEMFARAIHNAS
ARRDKPFVAVNCGAFPSELIDSILFGHKKGAFTGAVSDKVGVFELAHSGTLFLDEFGELDSSAQVRLLRVLQDGKFTRLG
DSKERSSNFRLITATNRDLMADVSKGRFREDLFYRVAIGVLSLPPLRSRQSDLDHLADQFTAMLTQEYPSLGGKKISTAA
KKIISNHRWPGNIRELKATILRAALWSETAVLEDVDILRAILSTLQNSESILERDISKGVDIKSIIDLVERHYLERGLAF
TSGNKRKTALLLGYNNHQTLNNRLKKLGLENDND

Sequences:

>Translated_514_residues
MTCLLLLLINLNIGLGMNIINEKVLVSWIGGNDLESKASKMTGPIWATVSDVTFDRLELIYNYPSDLVTPYLELLTGNLS
CPVNARKADLSSPINFGEIYLAAERLLDVISCETIALSILLSPGTPAMQAVWILLGKTRFACNFYQASKEQGVNIVEIPF
KLTAEYIPSMTNLAPTEFGQLGLLDSDADPAFDDIITLDPEMLLLKAKAQVLASHEVSVLICGESGTGKEMFARAIHNAS
ARRDKPFVAVNCGAFPSELIDSILFGHKKGAFTGAVSDKVGVFELAHSGTLFLDEFGELDSSAQVRLLRVLQDGKFTRLG
DSKERSSNFRLITATNRDLMADVSKGRFREDLFYRVAIGVLSLPPLRSRQSDLDHLADQFTAMLTQEYPSLGGKKISTAA
KKIISNHRWPGNIRELKATILRAALWSETAVLEDVDILRAILSTLQNSESILERDISKGVDIKSIIDLVERHYLERGLAF
TSGNKRKTALLLGYNNHQTLNNRLKKLGLENDND
>Mature_513_residues
TCLLLLLINLNIGLGMNIINEKVLVSWIGGNDLESKASKMTGPIWATVSDVTFDRLELIYNYPSDLVTPYLELLTGNLSC
PVNARKADLSSPINFGEIYLAAERLLDVISCETIALSILLSPGTPAMQAVWILLGKTRFACNFYQASKEQGVNIVEIPFK
LTAEYIPSMTNLAPTEFGQLGLLDSDADPAFDDIITLDPEMLLLKAKAQVLASHEVSVLICGESGTGKEMFARAIHNASA
RRDKPFVAVNCGAFPSELIDSILFGHKKGAFTGAVSDKVGVFELAHSGTLFLDEFGELDSSAQVRLLRVLQDGKFTRLGD
SKERSSNFRLITATNRDLMADVSKGRFREDLFYRVAIGVLSLPPLRSRQSDLDHLADQFTAMLTQEYPSLGGKKISTAAK
KIISNHRWPGNIRELKATILRAALWSETAVLEDVDILRAILSTLQNSESILERDISKGVDIKSIIDLVERHYLERGLAFT
SGNKRKTALLLGYNNHQTLNNRLKKLGLENDND

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=311, Percent_Identity=40.5144694533762, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788550, Length=319, Percent_Identity=40.1253918495298, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=41.4330218068536, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI87082152, Length=263, Percent_Identity=44.106463878327, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1789087, Length=264, Percent_Identity=44.6969696969697, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1790437, Length=294, Percent_Identity=40.1360544217687, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1790299, Length=334, Percent_Identity=37.7245508982036, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789233, Length=233, Percent_Identity=42.4892703862661, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787583, Length=319, Percent_Identity=36.3636363636364, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI87081872, Length=293, Percent_Identity=38.5665529010239, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786524, Length=246, Percent_Identity=41.4634146341463, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789828, Length=416, Percent_Identity=30.0480769230769, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=28.8461538461538, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56634; Mature: 56503

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCLLLLLINLNIGLGMNIINEKVLVSWIGGNDLESKASKMTGPIWATVSDVTFDRLELI
CHHHEEEHHHCCCCCCCHHHCCEEEEEEECCCCHHHHHHHCCCCEEEEECHHHHHHHHHH
YNYPSDLVTPYLELLTGNLSCPVNARKADLSSPINFGEIYLAAERLLDVISCETIALSIL
HCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEE
LSPGTPAMQAVWILLGKTRFACNFYQASKEQGVNIVEIPFKLTAEYIPSMTNLAPTEFGQ
ECCCCHHHHHHHHHHCCHHHEEEHHHHHHHCCCEEEEECHHHHHHHCCCHHCCCCCCCCC
LGLLDSDADPAFDDIITLDPEMLLLKAKAQVLASHEVSVLICGESGTGKEMFARAIHNAS
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCC
ARRDKPFVAVNCGAFPSELIDSILFGHKKGAFTGAVSDKVGVFELAHSGTLFLDEFGELD
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCEEEHHHCCCC
SSAQVRLLRVLQDGKFTRLGDSKERSSNFRLITATNRDLMADVSKGRFREDLFYRVAIGV
CHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHH
LSLPPLRSRQSDLDHLADQFTAMLTQEYPSLGGKKISTAAKKIISNHRWPGNIRELKATI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
LRAALWSETAVLEDVDILRAILSTLQNSESILERDISKGVDIKSIIDLVERHYLERGLAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEE
TSGNKRKTALLLGYNNHQTLNNRLKKLGLENDND
ECCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TCLLLLLINLNIGLGMNIINEKVLVSWIGGNDLESKASKMTGPIWATVSDVTFDRLELI
HHHEEEHHHCCCCCCCHHHCCEEEEEEECCCCHHHHHHHCCCCEEEEECHHHHHHHHHH
YNYPSDLVTPYLELLTGNLSCPVNARKADLSSPINFGEIYLAAERLLDVISCETIALSIL
HCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEE
LSPGTPAMQAVWILLGKTRFACNFYQASKEQGVNIVEIPFKLTAEYIPSMTNLAPTEFGQ
ECCCCHHHHHHHHHHCCHHHEEEHHHHHHHCCCEEEEECHHHHHHHCCCHHCCCCCCCCC
LGLLDSDADPAFDDIITLDPEMLLLKAKAQVLASHEVSVLICGESGTGKEMFARAIHNAS
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCC
ARRDKPFVAVNCGAFPSELIDSILFGHKKGAFTGAVSDKVGVFELAHSGTLFLDEFGELD
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCEEEHHHCCCC
SSAQVRLLRVLQDGKFTRLGDSKERSSNFRLITATNRDLMADVSKGRFREDLFYRVAIGV
CHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHH
LSLPPLRSRQSDLDHLADQFTAMLTQEYPSLGGKKISTAAKKIISNHRWPGNIRELKATI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
LRAALWSETAVLEDVDILRAILSTLQNSESILERDISKGVDIKSIIDLVERHYLERGLAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEE
TSGNKRKTALLLGYNNHQTLNNRLKKLGLENDND
ECCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]